ViewGene
Locus Name:
GeneID:
Location:

GeneID:1509
TrivialName: PA14_57210
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_57210
PAO1 Ortholog Locus:PA4402
Sequence Length:1218
Protein Length:405
Start: 5097277
Stop: 5096060
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG07790.1|AE004856_1 (AE004856) glutamate N-acetyltransferase [Pseudomonas aeruginosa PAO1]
Identities = 405/405 (100%), Positives = 405/405 (100%)
Sequence:
ATGGCTGTCGGTCTTGGCCCCCTGTCCACCCTGCACCCGGTCCCCGGTTTCGAACTCGGCATCGCTTCCGCC
GGCATCAAGCGACCGGGGCGCAAAGACGTGGTGGTCATGCGCTGCGCCGAAGGCTCCAGCGTGGCCGGGGTG
TTCACCCTGAACGCGTTCTGTGCCGCGCCGGTGACCCTGGCCAAGCAGCGTTTCCTCGGTGAGGTGCGCTAC
CTGTTGACCAATACCGGCAACGCCAACGCCGGTACCGGCGAGGCCGGCCTCGCCGCCGCCGCGCAAACCTGC
GCCAAGCTCGCCGAACTGGCCGGGGTCGCCGAGACTTCGGTGCTGCCATATTCCACCGGGGTGATCGGCGAA
CCCCTGCCGGTGGCCAAGATCGAGGCGGCGTTGCCTGCGGCCCTGGCCGACCTGGCCGAGGACCGCTGGGCC
GAAGCTGCCGCGGGAATCATGACCACCGATACCCTGCCCAAGGGCGCCAGCCGCCAGTTCGTGCACGATGGC
GTGACCGTCACCGTCACCGGGATCAGCAAGGGCGCCGGGATGATCAAGCCGAACATGGCGACCATGCTCGGT
TATATCGCCACCGACGCCAAGGTCGCCCAGGGCGTGCTGCAGGATCTGTTGCGCGACGCCGCCAACAAGTCC
TTCAACCGCATCACCATCGACGGCGATACCTCCACCAACGACTGCTGCATGCTGATCGCCACCGGTCGCGCG
GCGCTGCCGGAAGTCACCCAGGCCAGCGGCGCGCTGTTCGCCGCGTTGAAGCAAGCGGTACTGGAGGTTTCC
ATGGAGCTGGCCCAGGCCATCGTTCGCGACGGCGAGGGCGCGACCAAGTTCGTCACTGTCCAGGTCAACGGC
GGCGCCACTCACCAGGAGTGCCTGGATGTCGGCTACGCCGTGGCCCACTCGCCGCTGATCAAGACCGCGCTG
TTCGCCTCCGACCCGAACTGGGGACGCATCCTCGCCGCGGTCGGACGTGCCGGCGTGGCCAATCTGGACGTG
AGCAAGATCGACGTGTTCCTTGGCGACGTCTGTATCGCCAGCCGTGGCGGCCGGGCGGCCAGTTACACCGAG
GAGCAGGGCGCGGCGGTGATGGCCcaGGCCGAGATCGGCATCCGCATCGAACTGGGACGCGGCACCTGCAGC
GAGACCATCTGGACCACCGACCTGTCCCACGAATACGTGAAGATCAACGCAGAGTACCGCACCTGA
Translation:
MAVGLGPLSTLHPVPGFELGIASAGIKRPGRKDVVVMRCAEGSSVAGVFTLNAFCAAPVTLAKQRFLGEVRY
LLTNTGNANAGTGEAGLAAAAQTCAKLAELAGVAETSVLPYSTGVIGEPLPVAKIEAALPAALADLAEDRWA
EAAAGIMTTDTLPKGASRQFVHDGVTVTVTGISKGAGMIKPNMATMLGYIATDAKVAQGVLQDLLRDAANKS
FNRITIDGDTSTNDCCMLIATGRAALPEVTQASGALFAALKQAVLEVSMELAQAIVRDGEGATKFVTVQVNG
GATHQECLDVGYAVAHSPLIKTALFASDPNWGRILAAVGRAGVANLDVSKIDVFLGDVCIASRGGRAASYTE
EQGAAVMAQAEIGIRIELGRGTCSETIWTTDLSHEYVKINAEYRT*
AnnotationID:1518GeneID:1509
AnnotatorUID: diggins
Modification Date/Time: 2005-05-02 14:02:42
Gene Name:argJ
Confidence Code:2
GeneProduct:N-acetylglutamate synthase
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(2) Amino acid biosynthesis and metabolism
Alternate Gene Product Name:putative glutamate/ornithine acetyltransferase
Functional Category Confidence Code:5
COGs:COG1364,pfam01960,KOG2786
Secondary Functional Category(ies):
EC Number:2.3.1.35
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAO57848.1| (AE016871) glutamate N-acetyltransferase/amino-acid
           acetyltransferase [Pseudomonas syringae pv. tomato str.
           DC3000]
          Length = 405

 Score =  680 bits (1754), Expect = 0.0
 Identities = 343/405 (84%), Positives = 366/405 (90%)

>emb|CAD86125.1| (BX321863) ArgJ family [Nitrosomonas europaea ATCC 19718]
          Length = 409

 Score =  466 bits (1200), Expect = e-131
 Identities = 240/409 (58%), Positives = 298/409 (72%), Gaps = 4/409 (0%)

>emb|CAE34789.1| (BX640450) arginine biosynthesis bifunctional protein [Bordetella
           bronchiseptica]
          Length = 408

 Score =  429 bits (1104), Expect = e-119
 Identities = 229/399 (57%), Positives = 287/399 (71%), Gaps = 3/399 (0%)








 
Structural Features:
COG1364, ArgJ, N-acetylglutamate synthase (N-acetylornithine aminotransferase) 
[Amino acid transport and metabolism]
CD-Length = 404 residues,  98.3% aligned
Score =  467 bits (1203), Expect = 2e-132
Identities = 190/401 (47%), Positives = 258/401 (64%), Gaps = 6/401 (1%)

pfam01960, ArgJ, ArgJ family. 
CD-Length = 384 residues, 100.0% aligned
Score =  381 bits (979), Expect = 2e-106
Identities = 170/391 (43%), Positives = 240/391 (61%), Gaps = 9/391 (2%)

KOG2786, Putative glutamate/ornithine acetyltransferase 
[Amino acid transport and metabolism]
CD-Length = 431 residues,  93.0% aligned
Score =  298 bits (765), Expect = 1e-81
Identities = 160/402 (39%), Positives = 227/402 (56%), Gaps = 13/402 (3%)






   
Genomic Context:






   
Comment:


















    ReferenceID:3021




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:20167




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:27895




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:1509_cdd.html


    		




 
URL:

















    ReferenceID:32708




  
Author/Investigator(s):
        O'Reilly M, Devine KM.





  
Title:
        Sequence and analysis of the citrulline biosynthetic operon argC-F from Bacillus subtilis.




  
PubMed:
        8025667


MedLine:
        




    
Source:
Microbiology. 1994 May;140 ( Pt 5):1023-5.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=8025667

















    ReferenceID:32709




  
Author/Investigator(s):
        Martin PR, Mulks MH.





  
Title:
        Sequence analysis and complementation studies of the argJ gene encoding ornithine acetyltransferase from Neisseria gonorrhoeae.




  
PubMed:
        1339419


MedLine:
        




    
Source:
J Bacteriol. 1992 Apr;174(8):2694-701.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=1339419









           
Homologs By Global Alignment 

           

       



     
Gene ID:1509
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
2689
                           PA4402_tr
                           translation of PA4402
                           405
                           99.99
                           99.99
                           0
                           2036.0
     

     
     
84876
                           gb|AAG07790.1|AE004856_1
                           (AE004856) glutamate N-acetyltransferase [Pseudomonas aeruginosa PAO1]
                           405
                           99.99
                           99.99
                           0
                           2036.0
     

     
     
84877
                           gb|AAN66969.1|AE016325_5
                           (AE016779) glutamate N-acetyltransferase/amino-acid acetyltransferase [Pseudomonas putida KT2440]
                           405
                           86.41
                           90.61
                           0
                           1762.0
     

     
     
84878
                           gb|AAO57848.1|
                           (AE016871) glutamate N-acetyltransferase/amino-acid acetyltransferase [Pseudomonas syringae pv. tomato str. DC3000]
                           405
                           84.69
                           90.37
                           0
                           1754.0
     

     
     
84879
                           emb|CAD86125.1|
                           (BX321863) ArgJ family [Nitrosomonas europaea ATCC 19718]
                           409
                           58.67
                           72.86
                           4
                           1205.0
     

     






      

          
GC ORFID: 44511How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA4402Glimmer Score: 

          
Start: 4360953Stop: 4359736

          
Length: 1218


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA4402 translation of PA4402Homolog Bit Score 788.0

          
Other Homologs:  psyr_15may02_Scaffold1_revised_gene1137 (ORF: BLASTX 681.0), t_PA4402 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGGCTGTCGGTCTTGGCCCCCTGTCCACCCTGCACCCGGTCCCCGGTTTCGAACTCGGCATCGCTTCCGCC 
GGCATCAAGCGACCGGGGCGCAAAGACGTGGTGGTCATGCGCTGCGCCGAAGGCTCCAGCGTGGCCGGGGTG 
TTCACCCTGAACGCGTTCTGTGCCGCGCCGGTGACCCTGGCCAAGCAGCGTTTCCTCGGTGAGGTGCGCTAC 
CTGTTGACCAATACCGGCAACGCCAACGCCGGTACCGGCGAGGCCGGCCTCGCCGCCGCCGCGCAAACCTGC 
GCCAAGCTCGCCGAACTGGCCGGGGTCGCCGAGACTTCGGTGCTGCCATATTCCACCGGGGTGATCGGCGAA 
CCCCTGCCGGTGGCCAAGATCGAGGCGGCGTTGCCTGCGGCCCTGGCCGACCTGGCCGAGGACCGCTGGGCC 
GAAGCTGCCGCGGGAATCATGACCACCGATACCCTGCCCAAGGGCGCCAGCCGCCAGTTCGTGCACGATGGC 
GTGACCGTCACCGTCACCGGGATCAGCAAGGGCGCCGGGATGATCAAGCCGAACATGGCGACCATGCTCGGT 
TATATCGCCACCGACGCCAAGGTCGCCCAGGGCGTGCTGCAGGATCTGTTGCGCGACGCCGCCAACAAGTCC 
TTCAACCGCATCACCATCGACGGCGATACCTCCACCAACGACTGCTGCATGCTGATCGCCACCGGTCGCGCG 
GCGCTGCCGGAAGTCACCCAGGCCAGCGGCGCGCTGTTCGCCGCGTTGAAGCAAGCGGTACTGGAGGTTTCC 
ATGGAGCTGGCCCAGGCCATCGTTCGCGACGGCGAGGGCGCGACCAAGTTCGTCACTGTCCAGGTCAACGGC 
GGCGCCACTCACCAGGAGTGCCTGGATGTCGGCTACGCCGTGGCCCACTCGCCGCTGATCAAGACCGCGCTG 
TTCGCCTCCGACCCGAACTGGGGACGCATCCTCGCCGCGGTCGGACGTGCCGGCGTGGCCAATCTGGACGTG 
AGCAAGATCGACGTGTTCCTTGGCGACGTCTGTATCGCCAGCCGTGGCGGCCGGGCGGCCAGTTACACCGAG 
GAGCAGGGCGCGGCGGTGATGGCCcaGGCCGAGATCGGCATCCGCATCGAACTGGGACGCGGCACCTGCAGC 
GAGACCATCTGGACCACCGACCTGTCCCACGAATACGTGAAGATCAACGCAGAGTACCGCACCTGA