ViewGene
Locus Name:
GeneID:
Location:

GeneID:1496
TrivialName: PA14_10790
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_10790
PAO1 Ortholog Locus:PA4110
Sequence Length:1194
Protein Length:397
Start: 933824
Stop: 932631
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAM08410.1|AF490770_1 (AF490770) cephalosporinase [Pseudomonas aeruginosa]
Identities = 394/397 (99%), Positives = 395/397 (99%)
Sequence:
ATGCGCGATACCAGATTCCCCTGCCTGTGCGGCATCGCCGCTTCCACACTGCTGTTCGCCACCACCCCGGCC
ATTGCCGATGAGGCCCCGGCGGATCGCCTGAAGGCACTGGTCGACGCCGCCGTACAACCGGTGATGAAGGCC
AATGACATTCCGGGCCTGACCGTAGCCATCAGCCTGAAAGGAGAACCGCATTACTTCAGCTATGGGCTGGCC
TCGAAAGAGGACGGCCGCCGGGTGACGCCGGAGACCCTGTTCGAGATCGGCTCGGTGAGCAAGACCTTCACC
GCCACCCTCGCCGGCTATGCCCTGGCCCAGGACAAGATGCGCCTCGACGACCGCGCCAGCCAGCACTGGCCG
GCGCTGCAGGGCAGCCGCTTCGACGGCATCAGCCTGCTCGACCTCGCGACCTATACCGCCGGCGGCTTGCCG
CTGCAGTTCCCCGACTCGGTGCAGAAGGACCGGGCACAGATCCGCGACTACTACCGCCAGTGGCAGCCGACC
TACGCGCCGGGCAGCCAGCGCCTCTATTCCAACCCGAGCATCGGCCTGTTCGGCTATCTCGCCGCGCGCAGC
CTGGGCCAGCCGTTCGAACGGCTCATGGAGCAGCAATTGTTCCCGGCACTGGGCCTCGAACAGACCCACCTC
GACGTGCCCGAGGCGGCGCTGGCGCAGTACGCCCAGGGCTACGGCAAGGACGACCGCCCGCTACGGGTCGGT
CCCGGCCCGCTGGATGCCGAAGGCTACGGGGTGAAGACCAGCGCGGCCGACCTGCTGCGCTTCGTCGATGCC
AACCTGCATCCGGAGCGCCTGGACAGGCCCTGGGCGCAGGCGCTCGATGCCACCCATCGCGGTTACTACAAG
GTCGGCGACATGACCCAGGGCCTGGGCTGGGAAGCCTACGACTGGCCGATCTCCCTGAAGCGCCTGCAGGCC
GGCAACTCGACGCCGATGGCGCTGCAACCACACAGGATCGCCAGGCTGCCCGCGCCACAGGCGCTGGAGGGC
CAGCGCCTGCTGAACAAGACCGGCTCCACCAACGGCTTCGGCGCCTACGTGGCGTTCGTCCCGGGCCGCGAC
CTGGGCCTGGTGATCCTGGCCAACCGCAACTATCCCAATGCCGAGCGGGTGAAGATCGCCTACGCCATCCTC
AGCGGCCTGGAGCAGCAGGCCAAGGTGCCGCTGAAGCGCTGA
Translation:
MRDTRFPCLCGIAASTLLFATTPAIADEAPADRLKALVDAAVQPVMKANDIPGLTVAISLKGEPHYFSYGLA
SKEDGRRVTPETLFEIGSVSKTFTATLAGYALAQDKMRLDDRASQHWPALQGSRFDGISLLDLATYTAGGLP
LQFPDSVQKDRAQIRDYYRQWQPTYAPGSQRLYSNPSIGLFGYLAARSLGQPFERLMEQQLFPALGLEQTHL
DVPEAALAQYAQGYGKDDRPLRVGPGPLDAEGYGVKTSAADLLRFVDANLHPERLDRPWAQALDATHRGYYK
VGDMTQGLGWEAYDWPISLKRLQAGNSTPMALQPHRIARLPAPQALEGQRLLNKTGSTNGFGAYVAFVPGRD
LGLVILANRNYPNAERVKIAYAILSGLEQQAKVPLKR*
AnnotationID:1505GeneID:1496
AnnotatorUID: diggins
Modification Date/Time: 2005-03-30 11:11:17
Gene Name:ampC
Confidence Code:1
GeneProduct:cephalosporinase
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(1) Adaptation, protection
Alternate Gene Product Name:beta-lactamase precursor
Functional Category Confidence Code:5
COGs:pfam00144,COG1680
Secondary Functional Category(ies):
EC Number:3.5.2.6
Status:ACTIVE
Pathway:






 
Homology:
>emb|CAC49440.1| (AL603645) putative exported beta-lactamase protein [Sinorhizobium
           meliloti]
          Length = 396

 Score =  426 bits (1094), Expect = e-118
 Identities = 213/376 (56%), Positives = 271/376 (72%), Gaps = 1/376 (0%)

>emb|CAB76925.1| (AJ276030) Cephalosporinase [Aeromonas hydrophila]
          Length = 382

 Score =  419 bits (1076), Expect = e-116
 Identities = 212/376 (56%), Positives = 273/376 (72%), Gaps = 5/376 (1%)

>emb|CAA63264.1| (X92508) ES-beta-lactamase [Klebsiella pneumoniae]
          Length = 382

 Score =  414 bits (1063), Expect = e-115
 Identities = 208/375 (55%), Positives = 275/375 (73%), Gaps = 5/375 (1%)








 
Structural Features:
pfam00144, Beta-lactamase, Beta-lactamase.COG1680, AmpC, Beta-lactamase class C and 
other penicillin binding proteins [Defense mechanisms]
CD-Length = 324 residues, 100.0% aligned
Score =  207 bits (528), Expect = 2e-54
Identities = 116/357 (32%), Positives = 165/357 (46%), Gaps = 39/357 (10%)

COG1680, AmpC, Beta-lactamase class C and other penicillin binding proteins 
[Defense mechanisms]
CD-Length = 390 residues,  98.7% aligned
Score =  169 bits (429), Expect = 4e-43
Identities = 120/412 (29%), Positives = 177/412 (42%), Gaps = 45/412 (10%)






   
Genomic Context:






   
Comment:


















    ReferenceID:2995




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:20140




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:36951




  
Author/Investigator(s):
        Biochem J. 1990 Dec 15;272(3):627-31.





  
Title:
        Cloning, sequencing and analysis of the structural gene and regulatory region of the Pseudomonas aeruginosa chromosomal ampC beta-lactamase.




  
PubMed:
        2125210


MedLine:
        




    
Source:
Biochem J. 1990 Dec 15;272(3):627-31.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=2125210

















    ReferenceID:36952




  
Author/Investigator(s):
        Lodge J, Busby S, Piddock L.





  
Title:
        Investigation of the Pseudomonas aeruginosa ampR gene and its role at the chromosomal ampC beta-lactamase promoter.




  
PubMed:
        8405939


MedLine:
        




    
Source:
FEMS Microbiol Lett. 1993 Aug 1;111(2-3):315-20.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=94010221&dopt=Abstract

















    ReferenceID:38083




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:1496_cdd.html


    		




 
URL:









           
Homologs By Global Alignment 

           

       



     
Gene ID:1496
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
84355
                           gb|AAM08410.1|AF490770_1
                           (AF490770) cephalosporinase [Pseudomonas aeruginosa]
                           397
                           99.24
                           99.49
                           0
                           2052.0
     

     
     
84356
                           gb|AAM08942.1|
                           (AY083592) beta-lactamase precursor [Pseudomonas aeruginosa]
                           397
                           98.99
                           99.24
                           0
                           2048.0
     

     
     
2676
                           PA4110_tr
                           translation of PA4110
                           397
                           98.48
                           98.99
                           0
                           2041.0
     

     
     
84357
                           gb|AAG07497.1|AE004827_6
                           (AE004827) beta-lactamase precursor [Pseudomonas aeruginosa PAO1]
                           397
                           98.48
                           98.99
                           0
                           2041.0
     

     
     
84358
                           gb|AAM08945.1|
                           (AY083595) beta-lactamase precursor [Pseudomonas aeruginosa]
                           397
                           98.48
                           98.99
                           0
                           2041.0
     

     






      

          
GC ORFID: 44498How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA4110Glimmer Score: 

          
Start: 4020113Stop: 4021306

          
Length: 1194


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA4110 translation of PA4110Homolog Bit Score 790.0

          
Other Homologs:  t_PA4110 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGCGCGATACCAGATTCCCCTGCCTGTGCGGCATCGCCGCTTCCACACTGCTGTTCGCCACCACCCCGGCC 
ATTGCCGATGAGGCCCCGGCGGATCGCCTGAAGGCACTGGTCGACGCCGCCGTACAACCGGTGATGAAGGCC 
AATGACATTCCGGGCCTGACCGTAGCCATCAGCCTGAAAGGAGAACCGCATTACTTCAGCTATGGGCTGGCC 
TCGAAAGAGGACGGCCGCCGGGTGACGCCGGAGACCCTGTTCGAGATCGGCTCGGTGAGCAAGACCTTCACC 
GCCACCCTCGCCGGCTATGCCCTGGCCCAGGACAAGATGCGCCTCGACGACCGCGCCAGCCAGCACTGGCCG 
GCGCTGCAGGGCAGCCGCTTCGACGGCATCAGCCTGCTCGACCTCGCGACCTATACCGCCGGCGGCTTGCCG 
CTGCAGTTCCCCGACTCGGTGCAGAAGGACCGGGCACAGATCCGCGACTACTACCGCCAGTGGCAGCCGACC 
TACGCGCCGGGCAGCCAGCGCCTCTATTCCAACCCGAGCATCGGCCTGTTCGGCTATCTCGCCGCGCGCAGC 
CTGGGCCAGCCGTTCGAACGGCTCATGGAGCAGCAATTGTTCCCGGCACTGGGCCTCGAACAGACCCACCTC 
GACGTGCCCGAGGCGGCGCTGGCGCAGTACGCCCAGGGCTACGGCAAGGACGACCGCCCGCTACGGGTCGGT 
CCCGGCCCGCTGGATGCCGAAGGCTACGGGGTGAAGACCAGCGCGGCCGACCTGCTGCGCTTCGTCGATGCC 
AACCTGCATCCGGAGCGCCTGGACAGGCCCTGGGCGCAGGCGCTCGATGCCACCCATCGCGGTTACTACAAG 
GTCGGCGACATGACCCAGGGCCTGGGCTGGGAAGCCTACGACTGGCCGATCTCCCTGAAGCGCCTGCAGGCC 
GGCAACTCGACGCCGATGGCGCTGCAACCACACAGGATCGCCAGGCTGCCCGCGCCACAGGCGCTGGAGGGC 
CAGCGCCTGCTGAACAAGACCGGCTCCACCAACGGCTTCGGCGCCTACGTGGCGTTCGTCCCGGGCCGCGAC 
CTGGGCCTGGTGATCCTGGCCAACCGCAACTATCCCAATGCCGAGCGGGTGAAGATCGCCTACGCCATCCTC 
AGCGGCCTGGAGCAGCAGGCCAAGGTGCCGCTGAAGCGCTGA