ViewGene
Locus Name:
GeneID:
Location:

GeneID:1495
TrivialName: PA14_18430
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_18430
PAO1 Ortholog Locus:PA3549
Sequence Length:1176
Protein Length:391
Start: 1582197
Stop: 1581022
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG06937.1|AE004775_8 (AE004775) alginate o-acetyltransferase AlgJ [Pseudomonas aeruginosa PAO1]
Identities = 391/391 (100%), Positives = 391/391 (100%)
Sequence:
ATGACACAGAGCATTTCCCGCCCCCTGCAATACGCCTATATCGCCGCCTTCGGCGGGCTGCTGCTGGGGCTC
GCCGGATGGTCGCTGAAGAGCGTGCCGGGCTTCTCTGCCGCGGCCGATACGCCACTGCTCAACGGCAAGCTG
GCGCACGCCTTCGAAGCCCACTACGACAAGGAATTCCCGATCAAGCGCCTGGGCACCAACCTCTGGGCCGCA
CTCGACTACACGCTGTTCCACGAAGGCCGCCCCGGCGTGGTGATCGGCAAGGACGGCTGGCTGTTCACCGAC
GAAGAGTTCAAGCCGGCACCGAGCGGCCAGCAACTGGAAGACAACTGGGCACTGGTGCGCGGCGTGCAGCGC
GAGCTGAACCGGCGCGGCGTCAAGCTGGTGCTCGCGGTGATCCCGGCCAAGGCCCGCCTGTATCCCGAGCAC
ATCGGCCGCGAGCAGCCGGCCGCGCTGCACGACAGCCTCTACCAGGACTTCCTGGCCCGCGCCCGCGCCGCC
GGCATCGACAGTCCCGACCTGCTCGGCAGCCTGCGCCAGGCCAAGGACAACGGCGCGGTGTTCCTGCGCACC
GACACCCACTGGTCGCCGCTCGGCGCGGAAACCGTGGCCCAGCGCCTCGGCGCGGAGATCCGCGAGACGCAC
CTGCTGGATGTGCCGGCGCAGAACTTCGTCACCCGCGTCGGCGAAGAACGCACGCACAAGGGCGACCTGCTC
AGCTTCCTGCCGCTGGACCCGCTGTTCGACGAACTGCTGCCGCGTCCGGAGCAGTTGCAGCAACGCACCACC
GAAGCCGCGCCGGCACTGCCGGGCGGCCAGCAGTCCGGCGCGGGCGACGACCTCTTCGGCGATAGCCAGCAA
CCGCGCCTGGCGCTGGTCGGCACCAGCTACAGCGCCAACCCCAGGTGGAACTTCGAGGGCGCGCTGAAACAG
GCGCTGTCCGCCGACCTGATCAATTACGCGAAGGAAGGCAAGGGCCCCCTCGAACCGATGCTGGAACTGCTG
CAGGACGAAGGCTTCCGCAAGGACCCGCCGCAACTGCTGGTCTGGGAGTTTCCCGAGCGCTACCTGCCGATG
GCCAGCGACCTCAGCCAGTTCGACGCCGACTGGGTCGCCCAGCTGAAGGCCAGCGGCGGCCGCGATGAGCGC
CTGGCCGCCAGCCGCAACGATTGA
Translation:
MTQSISRPLQYAYIAAFGGLLLGLAGWSLKSVPGFSAAADTPLLNGKLAHAFEAHYDKEFPIKRLGTNLWAA
LDYTLFHEGRPGVVIGKDGWLFTDEEFKPAPSGQQLEDNWALVRGVQRELNRRGVKLVLAVIPAKARLYPEH
IGREQPAALHDSLYQDFLARARAAGIDSPDLLGSLRQAKDNGAVFLRTDTHWSPLGAETVAQRLGAEIRETH
LLDVPAQNFVTRVGEERTHKGDLLSFLPLDPLFDELLPRPEQLQQRTTEAAPALPGGQQSGAGDDLFGDSQQ
PRLALVGTSYSANPRWNFEGALKQALSADLINYAKEGKGPLEPMLELLQDEGFRKDPPQLLVWEFPERYLPM
ASDLSQFDADWVAQLKASGGRDERLAASRND*
AnnotationID:1504GeneID:1495
AnnotatorUID: danlee
Modification Date/Time: 2006-03-11 16:04:27
Gene Name:algJ
Confidence Code:1
GeneProduct:alginate o-acetyltransferase AlgJ
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(23) Secreted Factors (toxins, enzymes, alginate)
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:
Secondary Functional Category(ies):Adaptation, protection; Cell wall / LPS / capsule
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAO54759.1| (AE016860) alginate biosynthesis protein AlgJ [Pseudomonas syringae
           pv. tomato str. DC3000]
          Length = 391

 Score =  434 bits (1117), Expect = e-121
 Identities = 216/383 (56%), Positives = 277/383 (72%), Gaps = 7/383 (1%)








 
Structural Features:
CDD Search: No hits above 0.01 threshold.






   
Genomic Context:






   
Comment:


















    ReferenceID:2993




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:20138




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:34139




  
Author/Investigator(s):
        Franklin MJ, Ohman DE.





  
Title:
        Identification of algI and algJ in the Pseudomonas aeruginosa alginate biosynthetic gene cluster which are required for alginate O acetylation.




  
PubMed:
        8636017


MedLine:
        




    
Source:
J Bacteriol. 1996 Apr;178(8):2186-95.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=8636017

















    ReferenceID:34140




  
Author/Investigator(s):
        Franklin MJ, Douthit SA, McClure MA.





  
Title:
        Evidence that the algI/algJ gene cassette, required for O acetylation of Pseudomonas aeruginosa alginate, evolved by lateral gene transfer.




  
PubMed:
        15231808


MedLine:
        




    
Source:
J Bacteriol. 2004 Jul;186(14):4759-73.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=15231808









           
Homologs By Global Alignment 

           

       



     
Gene ID:1495
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
2675
                           PA3549_tr
                           translation of PA3549
                           391
                           99.99
                           99.99
                           0
                           2041.0
     

     
     
84349
                           gb|AAG06937.1|AE004775_8
                           (AE004775) alginate o-acetyltransferase AlgJ [Pseudomonas aeruginosa PAO1]
                           391
                           99.99
                           99.99
                           0
                           2041.0
     

     
     
84350
                           gb|AAB09782.1|
                           (U50202) AlgJ [Pseudomonas aeruginosa]
                           391
                           99.99
                           99.99
                           0
                           2041.0
     

     
     
84351
                           gb|AAC04569.1|
                           (AF027499) AlgV [Azotobacter vinelandii]
                           402
                           57.21
                           71.89
                           25
                           1179.0
     

     
     
84353
                           gb|AAN66903.1|AE016318_10
                           (AE016778) alginate O-acetylation protein AlgJ [Pseudomonas putida KT2440]
                           395
                           54.43
                           69.36
                           11
                           1113.0
     

     






      

          
GC ORFID: 44497How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA3549Glimmer Score: 

          
Start: 3371738Stop: 3372913

          
Length: 1176


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA3549 translation of PA3549Homolog Bit Score 790.0

          
Other Homologs:  psyr_15may02_Scaffold3_revised_gene5000 (ORF: BLASTX 429.0), t_PA3549 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGACACAGAGCATTTCCCGCCCCCTGCAATACGCCTATATCGCCGCCTTCGGCGGGCTGCTGCTGGGGCTC 
GCCGGATGGTCGCTGAAGAGCGTGCCGGGCTTCTCTGCCGCGGCCGATACGCCACTGCTCAACGGCAAGCTG 
GCGCACGCCTTCGAAGCCCACTACGACAAGGAATTCCCGATCAAGCGCCTGGGCACCAACCTCTGGGCCGCA 
CTCGACTACACGCTGTTCCACGAAGGCCGCCCCGGCGTGGTGATCGGCAAGGACGGCTGGCTGTTCACCGAC 
GAAGAGTTCAAGCCGGCACCGAGCGGCCAGCAACTGGAAGACAACTGGGCACTGGTGCGCGGCGTGCAGCGC 
GAGCTGAACCGGCGCGGCGTCAAGCTGGTGCTCGCGGTGATCCCGGCCAAGGCCCGCCTGTATCCCGAGCAC 
ATCGGCCGCGAGCAGCCGGCCGCGCTGCACGACAGCCTCTACCAGGACTTCCTGGCCCGCGCCCGCGCCGCC 
GGCATCGACAGTCCCGACCTGCTCGGCAGCCTGCGCCAGGCCAAGGACAACGGCGCGGTGTTCCTGCGCACC 
GACACCCACTGGTCGCCGCTCGGCGCGGAAACCGTGGCCCAGCGCCTCGGCGCGGAGATCCGCGAGACGCAC 
CTGCTGGATGTGCCGGCGCAGAACTTCGTCACCCGCGTCGGCGAAGAACGCACGCACAAGGGCGACCTGCTC 
AGCTTCCTGCCGCTGGACCCGCTGTTCGACGAACTGCTGCCGCGTCCGGAGCAGTTGCAGCAACGCACCACC 
GAAGCCGCGCCGGCACTGCCGGGCGGCCAGCAGTCCGGCGCGGGCGACGACCTCTTCGGCGATAGCCAGCAA 
CCGCGCCTGGCGCTGGTCGGCACCAGCTACAGCGCCAACCCCAGGTGGAACTTCGAGGGCGCGCTGAAACAG 
GCGCTGTCCGCCGACCTGATCAATTACGCGAAGGAAGGCAAGGGCCCCCTCGAACCGATGCTGGAACTGCTG 
CAGGACGAAGGCTTCCGCAAGGACCCGCCGCAACTGCTGGTCTGGGAGTTTCCCGAGCGCTACCTGCCGATG 
GCCAGCGACCTCAGCCAGTTCGACGCCGACTGGGTCGCCCAGCTGAAGGCCAGCGGCGGCCGCGATGAGCGC 
CTGGCCGCCAGCCGCAACGATTGA