ViewGene
Locus Name:
GeneID:
Location:

GeneID:1494
TrivialName: PA14_23160
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_23160
PAO1 Ortholog Locus:PA3176
Sequence Length:1215
Protein Length:404
Start: 2007789
Stop: 2006575
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG06564.1|AE004741_9 (AE004741) sodium/glutamate symporter GltS [Pseudomonas aeruginosa PAO1]
Identities = 401/404 (99%), Positives = 403/404 (99%)
Sequence:
ATGCCGACATTGCACCTGGACGCCCTGTCCACCACCGCCCTCGCCCTGCTCCTGCTCGCCCTCGGCAGCCAA
TTGAAGAAGCGCAGCCGCTGGCTGACACGTCTGTGCGTACCGTCGCCGGTGATCGCCGGCTTCGGCTTCGCC
TTCCTGGTCTGGCTGCTCAGGGATCGCGGCTGGCTCGACATCGGCCTCGACACCAGCATGCAGACGCCCCTG
ATGGTGGCGTTCTTCACCACCGTCGGCCTCGGTGGCAGCCTCGGCCTGCTGCGCAAGGGCGGCAAGACCCTG
CTGGTCTACCTGTCGGCCTGCTGGGCGCTGGCGATACTGCAGAACCTGATCGGCGTCGGCAGCGCCGGACTG
CTCGGCCTCGACCCGTTGCTCGGGATCATGGCCGGCGCGGTGTCGCTGGAAGGCGGCTTCGGCGCGGCGGCG
GCCTTCGGCCCGGTTGCGGAAGGGCTCGGTGCCCAGGGCGCGACCACCGTGGCGCTGGCCTCGGCCACCTTC
GGCATGGTCGCTGGCGGGCTGCTCGGCAGCCCGGTGGCGCGCTGGCTGATCGAACGCAACCGCCTGCCGGTG
CAGGCGGAAAGCGACAACCGGCTGGAAGCCCTCGGCCAGCAGGAGCGACGCCAGCATGCGGCGGCCACGCTG
GACGGCAATCTCCTGCTGCGCCTGCTGACCTGCGTGCTGCTGGTGATGGTGCTCGGCTTCTGGCTCGGCGAC
GCGCTCCAGGAACGCCTGGGCCTGGTGCTGCCCAGTTATGTCGGGGCGATGTTCATCGCCATCGTCCTGCGC
AACCTCGACGACCGGCTCGGCTGGCTGCGCATTCCGGACCACGCCGTCGGTACCCTGGGCGACGTCTGCCTG
GGCATCTTCCTGACCATGGCGATGATGAGCCTGAAGTTCTGGGAGCTGGAGAACCTCGGCCTGCCGCTGCTC
GGCGTACTGTTCATCCAGGTCGCGGTGCTGTTGCTGCTGACGATCTTCGCGCTGTTCCGCCTGCTCGGCCGC
AACTACGACGCGGCGGTGCTCTGCGCCGGCTTCCTCGGCCACGGCCTGGGCGCCACGCCCAACGCGGTGGCC
AACATGGGCGCGGTCTGCGAGCACTATCGGGTGTTCTCGCACAAGGCCTTCATCATCGTGCCCCTGTGCGGC
GCGGTACTGATCGATCTCGTCGCCATCCCGGCGATCACCTGGTTCATCAACGCCTTCAGTTGA
Translation:
MPTLHLDALSTTALALLLLALGSQLKKRSRWLTRLCVPSPVIAGFGFAFLVWLLRDRGWLDIGLDTSMQTPL
MVAFFTTVGLGGSLGLLRKGGKTLLVYLSACWALAILQNLIGVGSAGLLGLDPLLGIMAGAVSLEGGFGAAA
AFGPVAEGLGAQGATTVALASATFGMVAGGLLGSPVARWLIERNRLPVQAESDNRLEALGQQERRQHAAATL
DGNLLLRLLTCVLLVMVLGFWLGDALQERLGLVLPSYVGAMFIAIVLRNLDDRLGWLRIPDHAVGTLGDVCL
GIFLTMAMMSLKFWELENLGLPLLGVLFIQVAVLLLLTIFALFRLLGRNYDAAVLCAGFLGHGLGATPNAVA
NMGAVCEHYRVFSHKAFIIVPLCGAVLIDLVAIPAITWFINAFS*
AnnotationID:1503GeneID:1494
AnnotatorUID: diggins
Modification Date/Time: 2005-04-13 14:02:27
Gene Name:gltS
Confidence Code:2
GeneProduct: sodium/glutamate symporter
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(27) Transport of small molecules
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:pfam03616,COG0786
Secondary Functional Category(ies):
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAO36784.1| (AE015944) sodium/glutamate symport carrier protein [Clostridium
           tetani E88]
          Length = 427

 Score =  372 bits (954), Expect = e-102
 Identities = 198/409 (48%), Positives = 267/409 (65%), Gaps = 19/409 (4%)

>gb|AAO05565.1|AE016750_170 (AE016750) sodium/glutamate symporter [Staphylococcus epidermidis
           ATCC 12228]
          Length = 402

 Score =  306 bits (785), Expect = 1e-82
 Identities = 162/403 (40%), Positives = 254/403 (63%), Gaps = 4/403 (0%)








 
Structural Features:
pfam03616, Glt_symporter, Sodium/glutamate symporter.
CD-Length = 368 residues, 100.0% aligned
Score =  430 bits (1108), Expect = 1e-121
Identities = 177/369 (47%), Positives = 241/369 (65%), Gaps = 2/369 (0%)

COG0786, GltS, Na+/glutamate symporter 
[Amino acid transport and metabolism]
CD-Length = 404 residues,  99.8% aligned
Score =  413 bits (1063), Expect = 2e-116
Identities = 193/405 (47%), Positives = 262/405 (64%), Gaps = 4/405 (0%)






   
Genomic Context:






   
Comment:


















    ReferenceID:2991




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:20136




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:32368




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:1494_cdd.html


    		




 
URL:

















    ReferenceID:33955




  
Author/Investigator(s):
        Trotschel C, Kandirali S, Diaz-Achirica P, Meinhardt A, Morbach S, Kramer R, Burkovski A.





  
Title:
        GltS, the sodium-coupled L-glutamate uptake system of Corynebacterium glutamicum: identification of the corresponding gene and impact on L-glutamate production.




  
PubMed:
        12664155


MedLine:
        




    
Source:
Appl Microbiol Biotechnol. 2003 Feb;60(6):738-42. Epub 2002 Dec 18.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=12664155

















    ReferenceID:33956




  
Author/Investigator(s):
        Gal J, Szekeres S, Schnell R, Pongor S, Simoncsits A, Kalman M.





  
Title:
        A positive selection cloning system based on the gltS gene of Escherichia coli.




  
PubMed:
        9888982


MedLine:
        




    
Source:
Anal Biochem. 1999 Jan 15;266(2):235-8.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=9888982

















    ReferenceID:33957




  
Author/Investigator(s):
        Kalman M, Gentry DR, Cashel M.





  
Title:
        Characterization of the Escherichia coli K12 gltS glutamate permease gene.




  
PubMed:
        2017136


MedLine:
        




    
Source:
Mol Gen Genet. 1991 Mar;225(3):379-86.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=2017136

















    ReferenceID:33958




  
Author/Investigator(s):
        Deguchi Y, Yamato I, Anraku Y.





  
Title:
        Nucleotide sequence of gltS, the Na+/glutamate symport carrier gene of Escherichia coli B.




  
PubMed:
        2254324


MedLine:
        




    
Source:
J Biol Chem. 1990 Dec 15;265(35):21704-8.

Erratum in:
    * J Biol Chem 1991 Jun 15;266(17):11404.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=2254324

















    ReferenceID:33959




  
Author/Investigator(s):
        Deguchi Y, Yamato I, Anraku Y.





  
Title:
        Molecular cloning of gltS and gltP, which encode glutamate carriers of Escherichia coli B.




  
PubMed:
        2537813


MedLine:
        




    
Source:
J Bacteriol. 1989 Mar;171(3):1314-9.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=2537813









           
Homologs By Global Alignment 

           

       



     
Gene ID:1494
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
2674
                           PA3176_tr
                           translation of PA3176
                           404
                           99.25
                           99.75
                           0
                           2040.0
     

     
     
84337
                           gb|AAG06564.1|AE004741_9
                           (AE004741) sodium/glutamate symporter GltS [Pseudomonas aeruginosa PAO1]
                           404
                           99.25
                           99.75
                           0
                           2040.0
     

     
     
84338
                           gb|AAO36784.1|
                           (AE015944) sodium/glutamate symport carrier protein [Clostridium tetani E88]
                           441
                           45.12
                           61.22
                           51
                           969.0
     

     
     
84339
                           dbj|BAB43437.1|
                           (AP003137) SA2135 [Staphylococcus aureus subsp. aureus N315]
                           406
                           40.88
                           62.80
                           6
                           810.0
     

     
     
84340
                           dbj|BAB58507.1|
                           (AP003365) hypothetical protein [Staphylococcus aureus subsp. aureus Mu50]
                           406
                           40.88
                           62.80
                           6
                           810.0
     

     






      

          
GC ORFID: 44496How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA3176Glimmer Score: 

          
Start: 2946142Stop: 2947356

          
Length: 1215


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA3176 translation of PA3176Homolog Bit Score 790.0

          
Other Homologs:  t_PA3176 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGCCGACATTGCACCTGGACGCCCTGTCCACCACCGCCCTCGCCCTGCTCCTGCTCGCCCTCGGCAGCCAA 
TTGAAGAAGCGCAGCCGCTGGCTGACACGTCTGTGCGTACCGTCGCCGGTGATCGCCGGCTTCGGCTTCGCC 
TTCCTGGTCTGGCTGCTCAGGGATCGCGGCTGGCTCGACATCGGCCTCGACACCAGCATGCAGACGCCCCTG 
ATGGTGGCGTTCTTCACCACCGTCGGCCTCGGTGGCAGCCTCGGCCTGCTGCGCAAGGGCGGCAAGACCCTG 
CTGGTCTACCTGTCGGCCTGCTGGGCGCTGGCGATACTGCAGAACCTGATCGGCGTCGGCAGCGCCGGACTG 
CTCGGCCTCGACCCGTTGCTCGGGATCATGGCCGGCGCGGTGTCGCTGGAAGGCGGCTTCGGCGCGGCGGCG 
GCCTTCGGCCCGGTTGCGGAAGGGCTCGGTGCCCAGGGCGCGACCACCGTGGCGCTGGCCTCGGCCACCTTC 
GGCATGGTCGCTGGCGGGCTGCTCGGCAGCCCGGTGGCGCGCTGGCTGATCGAACGCAACCGCCTGCCGGTG 
CAGGCGGAAAGCGACAACCGGCTGGAAGCCCTCGGCCAGCAGGAGCGACGCCAGCATGCGGCGGCCACGCTG 
GACGGCAATCTCCTGCTGCGCCTGCTGACCTGCGTGCTGCTGGTGATGGTGCTCGGCTTCTGGCTCGGCGAC 
GCGCTCCAGGAACGCCTGGGCCTGGTGCTGCCCAGTTATGTCGGGGCGATGTTCATCGCCATCGTCCTGCGC 
AACCTCGACGACCGGCTCGGCTGGCTGCGCATTCCGGACCACGCCGTCGGTACCCTGGGCGACGTCTGCCTG 
GGCATCTTCCTGACCATGGCGATGATGAGCCTGAAGTTCTGGGAGCTGGAGAACCTCGGCCTGCCGCTGCTC 
GGCGTACTGTTCATCCAGGTCGCGGTGCTGTTGCTGCTGACGATCTTCGCGCTGTTCCGCCTGCTCGGCCGC 
AACTACGACGCGGCGGTGCTCTGCGCCGGCTTCCTCGGCCACGGCCTGGGCGCCACGCCCAACGCGGTGGCC 
AACATGGGCGCGGTCTGCGAGCACTATCGGGTGTTCTCGCACAAGGCCTTCATCATCGTGCCCCTGTGCGGC 
GCGGTACTGATCGATCTCGTCGCCATCCCGGCGATCACCTGGTTCATCAACGCCTTCAGTTGA