ViewGene
Locus Name:
GeneID:
Location:

GeneID:1493
TrivialName: PA14_24910
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_24910
PAO1 Ortholog Locus:PA3028
Sequence Length:1218
Protein Length:405
Start: 2175871
Stop: 2177088
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG06416.1|AE004728_2 (AE004728) molybdenum cofactor biosynthesis protein A2 [Pseudomonas aeruginosa PAO1]
Identities = 400/405 (98%), Positives = 401/405 (99%)
Sequence:
GTGAGCGGCTGCTGCGAACAGCCGGGGCTGATGCCGGTCGAGACGGCGCTGGAACGGCTCCTCGAACTGGCC
GCGCAAACGCCTATCGATACGTGTGAGCAGGTGCCCCTGGAGCAGGCTGGCGGCCGCGTGCTGGCGGTCGAC
CTGCTGGCCGGCCTGGACCTGCCGCCGTGGCCGAACAGCGCCATGGACGGCTACGCCTTGCGACTCGCCGAC
TGGAACGGCGAGCCACTGCCGGTCAGCCAGAAGATCTTTGCCGGACAGGCGCCGGAGCCGCTGCAAGCGGGT
ACCTGTGCGCGGATCTTCACCGGTGCGCCGCTGCCGGAAGGCGCCGATCTGGTAGAGATGCAGGAAAACGCC
GAAGCGCTCGACGATGGCCGCGTGCGCTTCACCCAGCCGTTGCGCGCGGGCCAGCATGTGCGCCCGCGTGGC
CAGGAAATCCGCCAGGGCGACCTGGTCCTGCCGGCCGGCACGCGCCTGCGCTCCATCGAACTGGGGCTGGCG
GCTTCGCTCGGCTGCGCGACCCTGGAGGTGCGACGCAAGCCGCGGGTGGCGCTGCTGTCCACCGGCGACGAG
CTGGTCGAGGCGGGCCAGCCGCTGCAGGCCGGGCAGATCTACAACAGCAACCGCCCGTTGCTCAAGGATTGG
CTGCAACGCCTGGGCTGCGACGTGCTCGACGCCGGTATCCTTCCCGACGACCTGGCACGTACCCGCGAGCGC
CTGGCCGGGTTGGCCGGGGTGGACCTTATCCTTTCCACTGGCGGCGTATCGGTGGGCGAGGCCGACTTCCTC
GGCATGGCGCTGCGCGAAGCCGGCGAGCTGACCCTCTGGAAGCTGGCGATAAAACCCGGCAAGCCGCTGATC
GTCGGCCAGTTCCAGGGCATCCCGGTGATCGGCCTGCCGGGCAATCCGGCTTCAACCCTGGTCACCTTCGCC
CTGCTGGCGCGTCCCTACCTGCTGCGCCGGCTCGGCGTGGCGCAGGTCGCGCCGCTGCGGGTGGAGGTACCG
GCCGGCTTCGCCTGGACCAAGGCGGGGCAGCGTCGCGAGTACCTGCGTGGTCGCCTGGAGCAGGGCCGCGCG
GTGCTCTATCCGAACCAGAGTTCCGGGGTACTGCGCAGCGCTTCCTGGGCCGAGGGGCTGGTGGAGGTCCGC
GAGGGACGTACCCTCGTGGAAGGCGAGGGCGTGGCGTTCATTCCGCTGAGCGAACTGCTCGACTGA
Translation:
MSGCCEQPGLMPVETALERLLELAAQTPIDTCEQVPLEQAGGRVLAVDLLAGLDLPPWPNSAMDGYALRLAD
WNGEPLPVSQKIFAGQAPEPLQAGTCARIFTGAPLPEGADLVEMQENAEALDDGRVRFTQPLRAGQHVRPRG
QEIRQGDLVLPAGTRLRSIELGLAASLGCATLEVRRKPRVALLSTGDELVEAGQPLQAGQIYNSNRPLLKDW
LQRLGCDVLDAGILPDDLARTRERLAGLAGVDLILSTGGVSVGEADFLGMALREAGELTLWKLAIKPGKPLI
VGQFQGIPVIGLPGNPASTLVTFALLARPYLLRRLGVAQVAPLRVEVPAGFAWTKAGQRREYLRGRLEQGRA
VLYPNQSSGVLRSASWAEGLVEVREGRTLVEGEGVAFIPLSELLD*
AnnotationID:1502GeneID:1493
AnnotatorUID: diggins
Modification Date/Time: 2005-04-12 06:06:36
Gene Name:moeA2
Confidence Code:2
GeneProduct:molybdenum cofactor biosynthesis protein A2
Cell Localization:()
Synonyms:moeA
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(4) Biosynthesis of cofactors, prosthetic groups and carriers
Alternate Gene Product Name:molybdopterin biosynthesis protein MoeA
Functional Category Confidence Code:5
COGs:COG0303
Secondary Functional Category(ies):
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAN67736.1|AE016404_11 (AE016782) molybdopterin biosynthesis MoeA protein [Pseudomonas
           putida KT2440]
          Length = 408

 Score =  578 bits (1489), Expect = e-164
 Identities = 294/395 (74%), Positives = 325/395 (82%)

>gb|AAO55864.1| (AE016864) molybdopterin biosynthesis protein MoeA [Pseudomonas
           syringae pv. tomato str. DC3000]
          Length = 403

 Score =  575 bits (1482), Expect = e-163
 Identities = 294/399 (73%), Positives = 329/399 (82%)

>emb|CAD15032.1| (AL646064) PUTATIVE MOLYBDOPTERIN BIOSYNTHESIS MOEA PROTEIN
           [Ralstonia solanacearum]
          Length = 419

 Score =  338 bits (866), Expect = 5e-92
 Identities = 198/412 (48%), Positives = 260/412 (63%), Gaps = 16/412 (3%)






 
Structural Features:
COG0303, MoeA, Molybdopterin biosynthesis enzyme [Coenzyme metabolism]
CD-Length = 404 residues, 100.0% aligned
Score =  396 bits (1018), Expect = 3e-111
Identities = 191/407 (46%), Positives = 254/407 (62%), Gaps = 11/407 (2%)






   
Genomic Context:






   
Comment:


















    ReferenceID:14373




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:20134




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:36020




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:1493_cdd.html


    		




 
URL:

















    ReferenceID:36485




  
Author/Investigator(s):
        Wang W, Zhang W, Lu J, Yang Y, Chiao J, Zhao G, Jiang W.





  
Title:
        MoeA, an enzyme in the molybdopterin synthesis pathway, is required for rifamycin SV production in Amycolatopsis mediterranei U32.




  
PubMed:
        12382055


MedLine:
        




    
Source:
Appl Microbiol Biotechnol. 2002 Oct;60(1-2):139-46. Epub 2002 Aug 22.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=12382055

















    ReferenceID:36486




  
Author/Investigator(s):
        Menendez C, Otto A, Igloi G, Nick P, Brandsch R, Schubach B, Bottcher B, Brandsch R.





  
Title:
        Molybdate-uptake genes and molybdopterin-biosynthesis genes on a bacterial plasmid--characterization of MoeA as a filament-forming protein with adenosinetriphosphatase activity.




  
PubMed:
        9428706


MedLine:
        




    
Source:
Eur J Biochem. 1997 Dec 1;250(2):524-31.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=9428706









           
Homologs By Global Alignment 

           

       



     
Gene ID:1493
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
2672
                           PA3028_tr
                           translation of PA3028
                           405
                           98.76
                           99.01
                           0
                           2040.0
     

     
     
84294
                           gb|AAG06416.1|AE004728_2
                           (AE004728) molybdenum cofactor biosynthesis protein A2 [Pseudomonas aeruginosa PAO1]
                           405
                           98.51
                           99.01
                           0
                           2037.0
     

     
     
84296
                           gb|AAO55864.1|
                           (AE016864) molybdopterin biosynthesis protein MoeA [Pseudomonas syringae pv. tomato str. DC3000]
                           406
                           72.41
                           81.28
                           4
                           1473.0
     

     
     
84295
                           gb|AAN67736.1|AE016404_11
                           (AE016782) molybdopterin biosynthesis MoeA protein [Pseudomonas putida KT2440]
                           408
                           72.30
                           80.39
                           3
                           1486.0
     

     
     
2673
                           PA3914_tr
                           translation of PA3914
                           411
                           50.60
                           64.47
                           10
                           912.0
     

     






      

          
GC ORFID: 44495How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA3028Glimmer Score: 

          
Start: 2778057Stop: 2776840

          
Length: 1218


          
Start Codon: GTGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA3028 translation of PA3028Homolog Bit Score 790.0

          
Other Homologs:  psyr_15may02_Scaffold1_revised_gene1105 (ORF: BLASTX 582.0), t_PA3914 (ORF: BLASTX 350.0), t_PA3028 (ORF: GLIMMER)

          
GC ORF Sequence

           
GTGAGCGGCTGCTGCGAACAGCCGGGGCTGATGCCGGTCGAGACGGCGCTGGAACGGCTCCTCGAACTGGCC 
GCGCAAACGCCTATCGATACGTGTGAGCAGGTGCCCCTGGAGCAGGCTGGCGGCCGCGTGCTGGCGGTCGAC 
CTGCTGGCCGGCCTGGACCTGCCGCCGTGGCCGAACAGCGCCATGGACGGCTACGCCTTGCGACTCGCCGAC 
TGGAACGGCGAGCCACTGCCGGTCAGCCAGAAGATCTTTGCCGGACAGGCGCCGGAGCCGCTGCAAGCGGGT 
ACCTGTGCGCGGATCTTCACCGGTGCGCCGCTGCCGGAAGGCGCCGATCTGGTAGAGATGCAGGAAAACGCC 
GAAGCGCTCGACGATGGCCGCGTGCGCTTCACCCAGCCGTTGCGCGCGGGCCAGCATGTGCGCCCGCGTGGC 
CAGGAAATCCGCCAGGGCGACCTGGTCCTGCCGGCCGGCACGCGCCTGCGCTCCATCGAACTGGGGCTGGCG 
GCTTCGCTCGGCTGCGCGACCCTGGAGGTGCGACGCAAGCCGCGGGTGGCGCTGCTGTCCACCGGCGACGAG 
CTGGTCGAGGCGGGCCAGCCGCTGCAGGCCGGGCAGATCTACAACAGCAACCGCCCGTTGCTCAAGGATTGG 
CTGCAACGCCTGGGCTGCGACGTGCTCGACGCCGGTATCCTTCCCGACGACCTGGCACGTACCCGCGAGCGC 
CTGGCCGGGTTGGCCGGGGTGGACCTTATCCTTTCCACTGGCGGCGTATCGGTGGGCGAGGCCGACTTCCTC 
GGCATGGCGCTGCGCGAAGCCGGCGAGCTGACCCTCTGGAAGCTGGCGATAAAACCCGGCAAGCCGCTGATC 
GTCGGCCAGTTCCAGGGCATCCCGGTGATCGGCCTGCCGGGCAATCCGGCTTCAACCCTGGTCACCTTCGCC 
CTGCTGGCGCGTCCCTACCTGCTGCGCCGGCTCGGCGTGGCGCAGGTCGCGCCGCTGCGGGTGGAGGTACCG 
GCCGGCTTCGCCTGGACCAAGGCGGGGCAGCGTCGCGAGTACCTGCGTGGTCGCCTGGAGCAGGGCCGCGCG 
GTGCTCTATCCGAACCAGAGTTCCGGGGTACTGCGCAGCGCTTCCTGGGCCGAGGGGCTGGTGGAGGTCCGC 
GAGGGACGTACCCTCGTGGAAGGCGAGGGCGTGGCGTTCATTCCGCTGAGCGAACTGCTCGACTGA