ViewGene
Locus Name:
GeneID:
Location:

GeneID:1478
TrivialName: PA14_65280
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_65280
PAO1 Ortholog Locus:PA4942
Sequence Length:1203
Protein Length:400
Start: 5818378
Stop: 5817176
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG08327.1|AE004907_5 (AE004907) protease subunit HflK [Pseudomonas aeruginosa PAO1]
Identities = 400/400 (100%), Positives = 400/400 (100%)
Sequence:
ATGGCTTGGAACGAGCCGGGTGACAACTCGAACAACAACGATCGAGACCCGTGGGGAGGCCGCCGCGGTGGT
GGTGGCGGCGGTGGTGACCGCAAGGGGCCGCCCGACCTGGATGAGGCTTTCCGCAAGCTTCAGGACAGCCTG
AACGGGCTGTTCGGTGGAAAAAAACGCAGTGGCAACGGTTCCGGCTCCGGTTCCGGCGGCAAGGGCGGTGGC
CTGGGCCTGTTCGGCATCGGCCTGGCGATCCTCGCCGTGCTCTGGCTGTACAACGCCATCTATGTGGTGGAC
GAGCAGGAGCAGGCGGTCATCCTGCGCTTCGGCAAGTATTACGAGACGGTCGGTCCCGGCCTGAACTTCTAC
TTCCCGCCGATCGACAAGCGCTTCCAGGAGAACGTCACCCGCGAGCGTGCGTACAGCAAGCAGGGGCAGATG
CTCACCGAGGACGAGAACATCGTCGAAGTGCCGCTGACCGTGCAGTACAAGATCAGCAACCTGCAGGACTTC
GTGCTCAACGTCGACCAGCCCGAGGTCAGCCTGCAGCAGGCGACCGAGAGCGCGCTGCGGCATGTCGCCGGC
TCCACCACCATGGACCGGATCCTCACCGAAGGCCGCGAGCAGATGGCGACCGAGGTGCGCGAGCGCCTGCAA
CGCTTCCTGGATACCTACAGGACCGGTATCACCGTGACCCAGGTGAACATCCAGAGCGCCCAGGCCCCGCGT
GAGGTGCAGGAAGCGTTCGACGACGTGATCCGGGCCCGCGAGGACGAGCAGCGCGAGAAGAACCAGGCCGAA
GCCTATGCCAACGGCGTGGTGCCGGAGGCGCGTGGCCAGGCCCAGCGCATCATCGAAGAAGCCAACGGTTAC
CGCGACGAGGTGATTTCGCGCGCCCAGGGTGAGGCGGATCGCTTCTCCAAGCTGCTGGTGGAATATCGCAAG
GCTCCGGAAGTGACGCGCGAGCGTCTGTACCTGGACACCATGCAGGAAGTCTTCAGCCAGACCAGCAAGGTG
CTGGTGACCGGTCAGCAGGGGCAGAACAACCTGCTCTACCTGCCGCTGGACAAGATGATCGATGGTCGTAAC
GCCGCACCCGCTACGGGTGCCGGTGCTTCCGCCGGTGGTGGCAGCGCCAGCGCTTCCGACCTGGGGTCGCGG
ATCGTCAACGATCTGCGCCAGCAAGACGCGCGTACGAGGGAGAGCCGCTGA
Translation:
MAWNEPGDNSNNNDRDPWGGRRGGGGGGGDRKGPPDLDEAFRKLQDSLNGLFGGKKRSGNGSGSGSGGKGGG
LGLFGIGLAILAVLWLYNAIYVVDEQEQAVILRFGKYYETVGPGLNFYFPPIDKRFQENVTRERAYSKQGQM
LTEDENIVEVPLTVQYKISNLQDFVLNVDQPEVSLQQATESALRHVAGSTTMDRILTEGREQMATEVRERLQ
RFLDTYRTGITVTQVNIQSAQAPREVQEAFDDVIRAREDEQREKNQAEAYANGVVPEARGQAQRIIEEANGY
RDEVISRAQGEADRFSKLLVEYRKAPEVTRERLYLDTMQEVFSQTSKVLVTGQQGQNNLLYLPLDKMIDGRN
AAPATGAGASAGGGSASASDLGSRIVNDLRQQDARTRESR*
AnnotationID:1487GeneID:1478
AnnotatorUID: diggins
Modification Date/Time: 2005-03-13 14:02:28
Gene Name:hflK
Confidence Code:2
GeneProduct:protease subunit HflK
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(26) Translation, post-translational modification, degradation
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:COG0330,pfam01145,smart00244
Secondary Functional Category(ies):related to phage, transposon, or plasmid
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAN70459.1|AE016687_6 (AE016792) HflK protein [Pseudomonas putida KT2440]
          Length = 405

 Score =  626 bits (1614), Expect = e-179
 Identities = 313/404 (77%), Positives = 355/404 (87%), Gaps = 15/404 (3%)

>gb|AAO58368.1| (AE016873) hflK protein [Pseudomonas syringae pv. tomato str.
           DC3000]
          Length = 395

 Score =  605 bits (1559), Expect = e-172
 Identities = 302/403 (74%), Positives = 347/403 (86%), Gaps = 11/403 (2%)








 
Structural Features:
COG0330, HflC, Membrane protease subunits, 
stomatin/prohibitin homologs 
[Posttranslational modification, protein turnover, chaperones]
CD-Length = 291 residues,  96.9% aligned
Score =  191 bits (485), Expect = 2e-49
Identities = 91/285 (31%), Positives = 147/285 (51%), Gaps = 12/285 (4%)

pfam01145, Band_7, SPFH domain / Band 7 family.
CD-Length = 174 residues, 100.0% aligned
Score =  115 bits (289), Expect = 9e-27
Identities = 57/178 (32%), Positives = 96/178 (53%), Gaps = 5/178 (2%)

smart00244, PHB, prohibitin homologues
CD-Length = 160 residues, 100.0% aligned
Score =  105 bits (264), Expect = 8e-24
Identities = 49/162 (30%), Positives = 85/162 (52%), Gaps = 3/162 (1%)






   
Genomic Context:






   
Comment:


















    ReferenceID:2959




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:20104




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:35132




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:1478_cdd.html


    		




 
URL:

















    ReferenceID:35904




  
Author/Investigator(s):
        Noble JA, Innis MA, Koonin EV, Rudd KE, Banuett F, Herskowitz I.





  
Title:
        The Escherichia coli hflA locus encodes a putative GTP-binding protein and two membrane proteins, one of which contains a protease-like domain.




  
PubMed:
        8248183


MedLine:
        




    
Source:
Proc Natl Acad Sci U S A. 1993 Nov 15;90(22):10866-70.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=8248183

















    ReferenceID:35906




  
Author/Investigator(s):
        Banuett F, Herskowitz I.





  
Title:
        Identification of polypeptides encoded by an Escherichia coli locus (hflA) that governs the lysis-lysogeny decision of bacteriophage lambda.




  
PubMed:
        3040675


MedLine:
        




    
Source:
J Bacteriol. 1987 Sep;169(9):4076-85.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=3040675









           
Homologs By Global Alignment 

           

       



     
Gene ID:1478
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
2656
                           PA4942_tr
                           translation of PA4942
                           400
                           99.99
                           99.99
                           0
                           2049.0
     

     
     
83887
                           gb|AAG08327.1|AE004907_5
                           (AE004907) protease subunit HflK [Pseudomonas aeruginosa PAO1]
                           400
                           99.99
                           99.99
                           0
                           2049.0
     

     
     
83888
                           gb|AAN70459.1|AE016687_6
                           (AE016792) HflK protein [Pseudomonas putida KT2440]
                           416
                           75.24
                           85.33
                           27
                           1629.0
     

     
     
83889
                           gb|AAO58368.1|
                           (AE016873) hflK protein [Pseudomonas syringae pv. tomato str. DC3000]
                           403
                           74.93
                           86.10
                           11
                           1570.0
     

     
     
83890
                           gb|AAL23183.1|
                           (AE008905) component of modulator for protease specific for FtsH phage lambda cII repressor [Salmonella typhimurium LT2]
                           427
                           44.49
                           60.65
                           35
                           880.0
     

     






      

          
GC ORFID: 44480How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA4942Glimmer Score: 

          
Start: 5082059Stop: 5080857

          
Length: 1203


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA4942 translation of PA4942Homolog Bit Score 793.0

          
Other Homologs:  psyr_15may02_Scaffold2_revised_gene3432 (ORF: BLASTX 601.0), t_PA4942 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGGCTTGGAACGAGCCGGGTGACAACTCGAACAACAACGATCGAGACCCGTGGGGAGGCCGCCGCGGTGGT 
GGTGGCGGCGGTGGTGACCGCAAGGGGCCGCCCGACCTGGATGAGGCTTTCCGCAAGCTTCAGGACAGCCTG 
AACGGGCTGTTCGGTGGAAAAAAACGCAGTGGCAACGGTTCCGGCTCCGGTTCCGGCGGCAAGGGCGGTGGC 
CTGGGCCTGTTCGGCATCGGCCTGGCGATCCTCGCCGTGCTCTGGCTGTACAACGCCATCTATGTGGTGGAC 
GAGCAGGAGCAGGCGGTCATCCTGCGCTTCGGCAAGTATTACGAGACGGTCGGTCCCGGCCTGAACTTCTAC 
TTCCCGCCGATCGACAAGCGCTTCCAGGAGAACGTCACCCGCGAGCGTGCGTACAGCAAGCAGGGGCAGATG 
CTCACCGAGGACGAGAACATCGTCGAAGTGCCGCTGACCGTGCAGTACAAGATCAGCAACCTGCAGGACTTC 
GTGCTCAACGTCGACCAGCCCGAGGTCAGCCTGCAGCAGGCGACCGAGAGCGCGCTGCGGCATGTCGCCGGC 
TCCACCACCATGGACCGGATCCTCACCGAAGGCCGCGAGCAGATGGCGACCGAGGTGCGCGAGCGCCTGCAA 
CGCTTCCTGGATACCTACAGGACCGGTATCACCGTGACCCAGGTGAACATCCAGAGCGCCCAGGCCCCGCGT 
GAGGTGCAGGAAGCGTTCGACGACGTGATCCGGGCCCGCGAGGACGAGCAGCGCGAGAAGAACCAGGCCGAA 
GCCTATGCCAACGGCGTGGTGCCGGAGGCGCGTGGCCAGGCCCAGCGCATCATCGAAGAAGCCAACGGTTAC 
CGCGACGAGGTGATTTCGCGCGCCCAGGGTGAGGCGGATCGCTTCTCCAAGCTGCTGGTGGAATATCGCAAG 
GCTCCGGAAGTGACGCGCGAGCGTCTGTACCTGGACACCATGCAGGAAGTCTTCAGCCAGACCAGCAAGGTG 
CTGGTGACCGGTCAGCAGGGGCAGAACAACCTGCTCTACCTGCCGCTGGACAAGATGATCGATGGTCGTAAC 
GCCGCACCCGCTACGGGTGCCGGTGCTTCCGCCGGTGGTGGCAGCGCCAGCGCTTCCGACCTGGGGTCGCGG 
ATCGTCAACGATCTGCGCCAGCAAGACGCGCGTACGAGGGAGAGCCGCTGA