ViewGene
Locus Name:
GeneID:
Location:

GeneID:1471
TrivialName: PA14_08830
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_08830
PAO1 Ortholog Locus:PA4265
Sequence Length:1194
Protein Length:397
Start: 757816
Stop: 759009
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG07665.1|AE004843_7 (AE004843) elongation factor Tu [Pseudomonas aeruginosa PAO1]
Identities = 397/397 (100%), Positives = 397/397 (100%)
Sequence:
GTGGCTAAGGAAAAATTCGAACGTAACAAACCGCACGTCAACGTCGGCACCATCGGTCACGTTGACCATGGC
AAGACCACTCTGACCGCTGCACTGACCAAGGTCTGCTCCGACACCTGGGGTGGTTCCGCTCGTGCTTTCGAT
CAGATCGACAACGCGCCGGAAGAAAAGGCCCGCGGTATCACCATCAACACCTCGCACGTTGAATACGATTCC
GCTGTTCGTCACTACGCCCACGTTGACTGCCCCGGTCACGCCGACTACGTGAAGAACATGATCACCGGTGCT
GCCCAGATGGACGGCGCGATCCTGGTTTGCTCGGCTGCCGACGGCCCCATGCCGCAGACCCGCGAGCACATC
CTGCTGTCCCGCCAGGTAGGCGTTCCCTACATCGTCGTGTTCCTGAACAAGGCTGACATGGTCGACGACGCC
GAGCTGCTGGAACTGGTCGAGATGGAAGTTCGCGATCTGCTGAACACCTACGACTTCCCGGGCGACGACACT
CCGATCATCATCGGTTCCGCGCTGATGGCGCTGGAAGGTAAAGATGACAACGGCATCGGCGTAAGCGCCGTG
CAGAAGCTGGTAGAGACCCTGGACTCCTACATTCCGGAGCCGGTTCGTGCCATCGACCAGCCGTTCCTGATG
CCGATCGAAGACGTGTTCTCGATCTCCGGCCGCGGTACCGTGGTAACCGGTCGTGTAGAGCGCGGCATCATC
AAGGTCCAGGAAGAGGTGGAAATCGTCGGCATCAAGGCGACCACCAAGACCACCTGCACCGGCGTTGAAATG
TTCCGCAAGCTGCTCGACGAAGGTCGTGCTGGTGAGAACGTTGGTATCCTGCTGCGCGGCACCAAGCGTGAA
GACGTAGAGCGTGGCCAGGTACTGGCCAAGCCGGGCACCATCAAGCCGCACACCAAGTTCGAGTGCGAAGTG
TACGTGCTGTCCAAGGAAGAAGGTGGTCGTCACACTCCGTTCTTCAAGGGCTACCGTCCGCAGTTCTACTTC
CGTACCACCGACGTGACCGGTAACTGCGAGCTGCCGGAAGGCGTAGAGATGGTAATGCCGGGCGACAACATC
AAGATGGTTGTCACCCTGATCGCTCCGATCGCCATGGAAGATGGCCTGCGCTTCGCGATCCGCGAAGGCGGC
CGTACCGTTGGCGCCGGCGTGGTTGCCAAGATCATCGAGTAA
Translation:
MAKEKFERNKPHVNVGTIGHVDHGKTTLTAALTKVCSDTWGGSARAFDQIDNAPEEKARGITINTSHVEYDS
AVRHYAHVDCPGHADYVKNMITGAAQMDGAILVCSAADGPMPQTREHILLSRQVGVPYIVVFLNKADMVDDA
ELLELVEMEVRDLLNTYDFPGDDTPIIIGSALMALEGKDDNGIGVSAVQKLVETLDSYIPEPVRAIDQPFLM
PIEDVFSISGRGTVVTGRVERGIIKVQEEVEIVGIKATTKTTCTGVEMFRKLLDEGRAGENVGILLRGTKRE
DVERGQVLAKPGTIKPHTKFECEVYVLSKEEGGRHTPFFKGYRPQFYFRTTDVTGNCELPEGVEMVMPGDNI
KMVVTLIAPIAMEDGLRFAIREGGRTVGAGVVAKIIE*
AnnotationID:1480GeneID:1471
AnnotatorUID: danlee
Modification Date/Time: 2006-03-16 13:01:19
Gene Name:tufA
Confidence Code:2
GeneProduct:elongation factor Tu
Cell Localization:()
Synonyms:PA4265
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(26) Translation, post-translational modification, degradation
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:COG0050,COG5256,pfam00009
Secondary Functional Category(ies):translation, ribosomal structure and biogenesis
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAN66070.1|AE016236_4 (AE016775) translation elongation factor Tu [Pseudomonas putida
           KT2440]
          Length = 397

 Score =  715 bits (1845), Expect = 0.0
 Identities = 349/397 (87%), Positives = 377/397 (94%)

>gb|AAO54166.1| (AE016858) translation elongation factor Tu [Pseudomonas syringae
           pv. tomato str. DC3000]
          Length = 397

 Score =  709 bits (1829), Expect = 0.0
 Identities = 345/396 (87%), Positives = 376/396 (94%)

>gb|AAM84066.1|AE013648_8 (AE013648) protein chain elongation factor EF-Tu [Yersinia pestis
           KIM]
          Length = 394

 Score =  676 bits (1745), Expect = 0.0
 Identities = 335/397 (84%), Positives = 362/397 (91%), Gaps = 3/397 (0%)






 
Structural Features:
COG0050, TufB, GTPases - translation elongation factors 
[Translation, ribosomal structure and biogenesis]
CD-Length = 394 residues, 100.0% aligned
Score =  710 bits (1833), Expect = 0.0
Identities = 327/397 (82%), Positives = 354/397 (89%), Gaps = 3/397 (0%)

COG5256, TEF1, Translation elongation factor EF-1alpha (GTPase) 
[Translation, ribosomal structure and biogenesis]
CD-Length = 428 residues,  99.1% aligned
Score =  234 bits (599), Expect = 1e-62
Identities = 136/437 (31%), Positives = 205/437 (46%), Gaps = 62/437 (14%)

pfam00009, GTP_EFTU, Elongation factor Tu GTP binding domain. 
CD-Length = 211 residues, 100.0% aligned
Score =  220 bits (563), Expect = 2e-58
Identities = 88/211 (41%), Positives = 119/211 (56%), Gaps = 13/211 (6%)






   
Genomic Context:






   
Comment:


















    ReferenceID:14359




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:20090




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:36635




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:1471_cdd.html


    		




 
URL:

















    ReferenceID:36758




  
Author/Investigator(s):
        An G, Friesen JD.





  
Title:
        The nucleotide sequence of tufB and four nearby tRNA structural genes of Escherichia coli.




  
PubMed:
        7011904


MedLine:
        




    
Source:
Gene. 1980 Dec;12(1-2):33-9.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=7011904

















    ReferenceID:36761




  
Author/Investigator(s):
        Yokota T, Sugisaki H, Takanami M, Kaziro Y.





  
Title:
        The nucleotide sequence of the cloned tufA gene of Escherichia coli.




  
PubMed:
        7011903


MedLine:
        




    
Source:
Gene. 1980 Dec;12(1-2):25-31.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=7011903









           
Homologs By Global Alignment 

           

       



     
Gene ID:1471
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
2643
                           PA4265_tr
                           translation of PA4265
                           397
                           99.99
                           99.99
                           0
                           2054.0
     

     
     
2644
                           PA4277_tr
                           translation of PA4277
                           397
                           99.74
                           99.99
                           0
                           2051.0
     

     
     
83088
                           gb|AAG07665.1|AE004843_7
                           (AE004843) elongation factor Tu [Pseudomonas aeruginosa PAO1]
                           397
                           99.74
                           99.99
                           0
                           2051.0
     

     
     
83089
                           gb|AAG07653.1|AE004842_2
                           (AE004842) elongation factor Tu [Pseudomonas aeruginosa PAO1]
                           397
                           99.74
                           99.99
                           0
                           2051.0
     

     
     
83090
                           gb|AAN66070.1|AE016236_4
                           (AE016775) translation elongation factor Tu [Pseudomonas putida KT2440]
                           397
                           87.65
                           94.96
                           0
                           1841.0
     

     






      

          
GC ORFID: 44473How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA4265Glimmer Score: 

          
Start: 4196123Stop: 4194930

          
Length: 1194


          
Start Codon: GTGTruncated Start:

          
Stop Codon: TAATruncated Stop: 

          
Homolog: t_PA4265 translation of PA4265Homolog Bit Score 795.0

          
Other Homologs:  t_PA4277 (ORF: BLASTX 794.0), psyr_15may02_Scaffold4_revised_gene5182 (ORF: BLASTX 709.0), t_PA4265 (ORF: GLIMMER)

          
GC ORF Sequence

           
GTGGCTAAGGAAAAATTCGAACGTAACAAACCGCACGTCAACGTCGGCACCATCGGTCACGTTGACCATGGC 
AAGACCACTCTGACCGCTGCACTGACCAAGGTCTGCTCCGACACCTGGGGTGGTTCCGCTCGTGCTTTCGAT 
CAGATCGACAACGCGCCGGAAGAAAAGGCCCGCGGTATCACCATCAACACCTCGCACGTTGAATACGATTCC 
GCTGTTCGTCACTACGCCCACGTTGACTGCCCCGGTCACGCCGACTACGTGAAGAACATGATCACCGGTGCT 
GCCCAGATGGACGGCGCGATCCTGGTTTGCTCGGCTGCCGACGGCCCCATGCCGCAGACCCGCGAGCACATC 
CTGCTGTCCCGCCAGGTAGGCGTTCCCTACATCGTCGTGTTCCTGAACAAGGCTGACATGGTCGACGACGCC 
GAGCTGCTGGAACTGGTCGAGATGGAAGTTCGCGATCTGCTGAACACCTACGACTTCCCGGGCGACGACACT 
CCGATCATCATCGGTTCCGCGCTGATGGCGCTGGAAGGTAAAGATGACAACGGCATCGGCGTAAGCGCCGTG 
CAGAAGCTGGTAGAGACCCTGGACTCCTACATTCCGGAGCCGGTTCGTGCCATCGACCAGCCGTTCCTGATG 
CCGATCGAAGACGTGTTCTCGATCTCCGGCCGCGGTACCGTGGTAACCGGTCGTGTAGAGCGCGGCATCATC 
AAGGTCCAGGAAGAGGTGGAAATCGTCGGCATCAAGGCGACCACCAAGACCACCTGCACCGGCGTTGAAATG 
TTCCGCAAGCTGCTCGACGAAGGTCGTGCTGGTGAGAACGTTGGTATCCTGCTGCGCGGCACCAAGCGTGAA 
GACGTAGAGCGTGGCCAGGTACTGGCCAAGCCGGGCACCATCAAGCCGCACACCAAGTTCGAGTGCGAAGTG 
TACGTGCTGTCCAAGGAAGAAGGTGGTCGTCACACTCCGTTCTTCAAGGGCTACCGTCCGCAGTTCTACTTC 
CGTACCACCGACGTGACCGGTAACTGCGAGCTGCCGGAAGGCGTAGAGATGGTAATGCCGGGCGACAACATC 
AAGATGGTTGTCACCCTGATCGCTCCGATCGCCATGGAAGATGGCCTGCGCTTCGCGATCCGCGAAGGCGGC 
CGTACCGTTGGCGCCGGCGTGGTTGCCAAGATCATCGAGTAA