ViewGene
Locus Name:
GeneID:
Location:

GeneID:147
TrivialName: PA14_17500
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_17500
PAO1 Ortholog Locus:PA3620
Sequence Length:2568
Protein Length:855
Start: 1503484
Stop: 1500917
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG07008.1|AE004782_6 (AE004782) DNA mismatch repair protein MutS [Pseudomonas aeruginosa PAO1]
Identities = 854/855 (99%), Positives = 854/855 (99%)
Sequence:
ATGACCGACCTCTCTCAGCACACGCCAATGATGCAGCAGTACTTCAAACTGAAGCATCAGCACCCCGACCAA
TTGATGTTCTATCGCATGGGCGACTTCTACGAGCTGTTCTACGAGGACGCGAAGAAGGCCGCCAAGCTGCTC
GACATCACCCTGACCGCGCGCGGCCAGTCCGGCGGCAAGGCGATCCCGATGGCAGGCATTCCCTTCCATTCG
GCGGAGGGCTACCTGGCCAAGCTGGTCAAGCTCGGCGAGTCGGTGGCGATCTGCGAGCAGATCGGCGACCCG
GCCACCAGCAAGGGGCCGGTGGAGCGCCAGGTGGTGCGGATCATCACCCCCGGCACGGTGAGCGACGAGGCG
CTGCTCGACGAACGCCGCGACAACCTGCTGGCGGCAATCCTCGGCGACGAGCGCCTGTTCGGCCTCGCCGTG
CTGGACATCACCAGCGGCCGCTTCAGCGTCCAGGAGATAAAAGGCTGGGAAACCCTGCTGGCCGAACTGGAG
CGCCTCAACCCGGCCGAGCTGCTGATTCCCGACGACTGGCCACAGGGCCTGCCGGCGGAGAAGCGCCGCGGC
GTACGTCGCCGCGCGCCGTGGGACTTCGATCGCGACTCGGCGCACAAGAGCCTCTGCCAGCAATTCGGCACC
CAGGACCTGAAAGGCTTCGGCTGCCAGAACCTGACCCTGGCCATCGGCGCCGCCGGCTGCCTGCTCGCCTAC
GCCAAGGAAACCCAGCGTACCGCCCTGCCGCACCTGCGCAGCCTGCGCCACGACCGCCTCGATGACACGGTG
ATCCTCGACGGCGCCAGCCGCCGCAACCTGGAGCTGGATATCAACCTCAGCGGTGGCCGCGAGAACACCCTG
CAATCGGTGGTCGACCGCTGCCAGACCGCCATGGCCAGCCGCCTGATGAGCCGCTGGCTGAACCGTCCGCTG
CGTGACCGCGCGGTGCTGGAAGCCCGCCAGGAGTCCATCGCCTGCCTGCTGGAACGCTATCGCTTCGAGAAC
CTGCAACCGCAGCTCAAGGAAATCGGCGACCTCGAACGTATCCTCGCCCGCATCGGCCTGCGCAACGCCCGC
CCTCGCGACCTGGCGCGCCTGCGCGACGCGCTGGCGGCGTTGCCGGACCTGCAGAACGCCATGACCGAACTG
GAAGCGCCGCACCTGCAGGCGCTGGCCACCACCATCGGCACCTATCCCGAACTCGCCGAACTGCTGGCCAAG
GCGATCATCGACAACCCGCCGGCGGTGATCCGCGACGGTGGCGTGATCAAGACCGGCTATGACGCCGAGCTG
GACGAGCTGCAGGCGCTGAGCGAAAACGCCGGGCAATTCCTGATGGACCTGGAAGCGCGCGAGAAGGCCCGC
ACCGGCCTGCCCAACCTGAAGGTCGGCTACAACCGCATCCATGGCTACTTCATCGAGCTGCCACGGGTGCAG
GCCGAACAGGCGCCGGCCGACTACATCCGCCGGCAGACCCTGAAAGGCGCCGAGCGCTTCATCACGCCGGAA
CTGAAGGCCTTCGAGGACAAGGCGCTGTCGGCCCAGAGCCGCGCCCTGGCCCGCGAGAAGGCACTCTACGAA
GAGCTGCTGGAACGTCTGATCGGCCACCTCGCTCCGCTCCAGGACAGCGCCTCGGCGCTGGCGGAGCTGGAC
GTGCTGGCGAACCTCGCCGAACGCGCGCTGAACCTCGACCTGAATCGCCCGCGGTTCGTCGAACACACCTGC
CTGCACATCGAGCAGGGCCGCCATCCGGTGGTCGAGCAGGTGCTGGAGACGCCGTTCGTGGCCAACGACCTG
GCGCTGGATGCCGACACCCGGATGCTGGTGATCACCGGTCCGAACATGGGCGGTAAATCCACCTACATGCGG
CAAACCGCGCTGATCGTGCTGCTTGCGCATATCGGCAGCTTCGTTCCGGCTGCGCGCTGCGAGCTGTCCCTG
GTGGACCGCATCTTCACCCGCATCGGCTCGTCCGACGACCTTGCCGGCGGCCGCTCGACCTTCATGGTGGAG
ATGAGCGAAACCGCCAACATCCTGCACAACGCCACTGACAAGAGCCTGGTGCTGATGGATGAGGTCGGCCGC
GGCACCAGCACCTTCGACGGCCTGTCGCTGGCCTGGGCAGCGGCCGAGGACCTGGCCCGGACTCGCGCCTTC
ACCCTGTTCGCCACCCACTACTTCGAGCTGACCGTACTGCCGGAAAGCCAGCCCGCGGTAGCCAACGTGCAC
CTGAACGCGACCGAGCACAATGAACGCATCGTGTTCCTGCACCATGTACTGCCGGGACCGGCGAGCCAGAGC
TACGGCCTCGCGGTGGCCCAGTTGGCCGGCGTGCCGACTCCGGTAATCCAGCGCGCCCGCGAACACCTCAAG
CGCCTGGAAACCACCAGCCTGCCGCACGAGATGCCGAGCCAGCAGAGCGGCAAGCCCGCCTCGCCGATGCAG
AGCGACCTGTTCGCCAGCCTGCCGCACCCGGTGATCGATGAATTGTCGAGGATCAATCCCGACGATATCAGC
CCGCGGCAAGCTCTCGATCTGTTATATGCATGGAAGATGCGGGTCTGA
Translation:
MTDLSQHTPMMQQYFKLKHQHPDQLMFYRMGDFYELFYEDAKKAAKLLDITLTARGQSGGKAIPMAGIPFHS
AEGYLAKLVKLGESVAICEQIGDPATSKGPVERQVVRIITPGTVSDEALLDERRDNLLAAILGDERLFGLAV
LDITSGRFSVQEIKGWETLLAELERLNPAELLIPDDWPQGLPAEKRRGVRRRAPWDFDRDSAHKSLCQQFGT
QDLKGFGCQNLTLAIGAAGCLLAYAKETQRTALPHLRSLRHDRLDDTVILDGASRRNLELDINLSGGRENTL
QSVVDRCQTAMASRLMSRWLNRPLRDRAVLEARQESIACLLERYRFENLQPQLKEIGDLERILARIGLRNAR
PRDLARLRDALAALPDLQNAMTELEAPHLQALATTIGTYPELAELLAKAIIDNPPAVIRDGGVIKTGYDAEL
DELQALSENAGQFLMDLEAREKARTGLPNLKVGYNRIHGYFIELPRVQAEQAPADYIRRQTLKGAERFITPE
LKAFEDKALSAQSRALAREKALYEELLERLIGHLAPLQDSASALAELDVLANLAERALNLDLNRPRFVEHTC
LHIEQGRHPVVEQVLETPFVANDLALDADTRMLVITGPNMGGKSTYMRQTALIVLLAHIGSFVPAARCELSL
VDRIFTRIGSSDDLAGGRSTFMVEMSETANILHNATDKSLVLMDEVGRGTSTFDGLSLAWAAAEDLARTRAF
TLFATHYFELTVLPESQPAVANVHLNATEHNERIVFLHHVLPGPASQSYGLAVAQLAGVPTPVIQRAREHLK
RLETTSLPHEMPSQQSGKPASPMQSDLFASLPHPVIDELSRINPDDISPRQALDLLYAWKMRV*
AnnotationID:157GeneID:147
AnnotatorUID: diggins
Modification Date/Time: 2005-04-20 18:06:34
Gene Name:mutS
Confidence Code:2
GeneProduct:DNA mismatch repair protein MutS
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(11) DNA replication, recombination, modification and repair
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:COG0249,pfam00488,KOG0218
Secondary Functional Category(ies):
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAN67247.1|AE016351_9 (AE016780) DNA mismatch repair protein MutS [Pseudomonas putida
           KT2440]
          Length = 857

 Score = 1462 bits (3784), Expect = 0.0
 Identities = 738/854 (86%), Positives = 784/854 (91%), Gaps = 2/854 (0%)

>gb|AAN81743.1|AE016765_145 (AE016765) DNA mismatch repair protein mutS [Escherichia coli
           CFT073]
          Length = 853

 Score =  982 bits (2539), Expect = 0.0
 Identities = 508/850 (59%), Positives = 639/850 (75%), Gaps = 7/850 (0%)

>gb|AAP18070.1| (AE016987) methyl-directed mismatch repair protein [Shigella
           flexneri 2a str. 2457T]
          Length = 853

 Score =  981 bits (2536), Expect = 0.0
 Identities = 508/850 (59%), Positives = 639/850 (75%), Gaps = 7/850 (0%)



 






 
Structural Features:
COG0249, MutS, Mismatch repair ATPase (MutS family) 
[DNA replication, recombination, and repair]
CD-Length = 843 residues,  99.9% aligned
Score =  990 bits (2560), Expect = 0.0
Identities = 434/861 (50%), Positives = 586/861 (68%), Gaps = 29/861 (3%)

pfam00488, MutS_V, MutS domain V.
CD-Length = 234 residues,  99.1% aligned
Score =  383 bits (986), Expect = 8e-107
Identities = 151/232 (65%), Positives = 183/232 (78%), Gaps = 2/232 (0%)

KOG0218, Mismatch repair MSH3 [Replication, recombination and repair]
CD-Length = 1070 residues,  81.5% aligned
Score =  341 bits (876), Expect = 4e-94
Identities = 241/881 (27%), Positives = 397/881 (45%), Gaps = 96/881 (10%)






   
Genomic Context:






   
Comment:


















    ReferenceID:297




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:17439




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:31922




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:147_cdd.html


    		




 
URL:

















    ReferenceID:34168




  
Author/Investigator(s):
        Pezza RJ, Smania AM, Barra JL, Argarana CE.





  
Title:
        Nucleotides and heteroduplex DNA preserve the active conformation of Pseudomonas aeruginosa MutS by preventing protein oligomerization.




  
PubMed:
        11742532


MedLine:
        




    
Source:
Biochem J. 2002 Jan 1;361(Pt 1):87-95.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=11742532

















    ReferenceID:34169




  
Author/Investigator(s):
        Oliver A, Baquero F, Blazquez J.





  
Title:
        The mismatch repair system (mutS, mutL and uvrD genes) in Pseudomonas aeruginosa: molecular characterization of naturally occurring mutants.




  
PubMed:
        11952911


MedLine:
        




    
Source:
Mol Microbiol. 2002 Mar;43(6):1641-50.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=11952911









           
Homologs By Global Alignment 

           

       



     
Gene ID:147
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
268
                           PA3620_tr
                           translation of PA3620
                           855
                           99.88
                           99.88
                           0
                           4346.0
     

     
     
17791
                           gb|AAG07008.1|AE004782_6
                           (AE004782) DNA mismatch repair protein MutS [Pseudomonas aeruginosa PAO1]
                           855
                           99.88
                           99.88
                           0
                           4346.0
     

     
     
17792
                           gb|AAF36440.1|AF126491_1
                           (AF126491) mismatch repair system component MutS [Pseudomonas aeruginosa]
                           855
                           99.76
                           99.76
                           0
                           4338.0
     

     
     
17793
                           gb|AAF42850.1|
                           (AF220055) mismatch repair protein MutS [Pseudomonas aeruginosa]
                           856
                           99.76
                           99.76
                           1
                           4336.0
     

     
     
17794
                           gb|AAA16868.1|
                           (M63007) mutS [Azotobacter vinelandii]
                           855
                           87.13
                           93.33
                           0
                           3844.0
     

     






      

          
GC ORFID: 43149How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA3620Glimmer Score: 

          
Start: 3450453Stop: 3453020

          
Length: 2568


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA3620 translation of PA3620Homolog Bit Score 1678.0

          
Other Homologs:  psyr_15may02_Scaffold3_revised_gene4779 (ORF: BLASTX 1475.0), t_PA3620 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGACCGACCTCTCTCAGCACACGCCAATGATGCAGCAGTACTTCAAACTGAAGCATCAGCACCCCGACCAA 
TTGATGTTCTATCGCATGGGCGACTTCTACGAGCTGTTCTACGAGGACGCGAAGAAGGCCGCCAAGCTGCTC 
GACATCACCCTGACCGCGCGCGGCCAGTCCGGCGGCAAGGCGATCCCGATGGCAGGCATTCCCTTCCATTCG 
GCGGAGGGCTACCTGGCCAAGCTGGTCAAGCTCGGCGAGTCGGTGGCGATCTGCGAGCAGATCGGCGACCCG 
GCCACCAGCAAGGGGCCGGTGGAGCGCCAGGTGGTGCGGATCATCACCCCCGGCACGGTGAGCGACGAGGCG 
CTGCTCGACGAACGCCGCGACAACCTGCTGGCGGCAATCCTCGGCGACGAGCGCCTGTTCGGCCTCGCCGTG 
CTGGACATCACCAGCGGCCGCTTCAGCGTCCAGGAGATAAAAGGCTGGGAAACCCTGCTGGCCGAACTGGAG 
CGCCTCAACCCGGCCGAGCTGCTGATTCCCGACGACTGGCCACAGGGCCTGCCGGCGGAGAAGCGCCGCGGC 
GTACGTCGCCGCGCGCCGTGGGACTTCGATCGCGACTCGGCGCACAAGAGCCTCTGCCAGCAATTCGGCACC 
CAGGACCTGAAAGGCTTCGGCTGCCAGAACCTGACCCTGGCCATCGGCGCCGCCGGCTGCCTGCTCGCCTAC 
GCCAAGGAAACCCAGCGTACCGCCCTGCCGCACCTGCGCAGCCTGCGCCACGACCGCCTCGATGACACGGTG 
ATCCTCGACGGCGCCAGCCGCCGCAACCTGGAGCTGGATATCAACCTCAGCGGTGGCCGCGAGAACACCCTG 
CAATCGGTGGTCGACCGCTGCCAGACCGCCATGGCCAGCCGCCTGATGAGCCGCTGGCTGAACCGTCCGCTG 
CGTGACCGCGCGGTGCTGGAAGCCCGCCAGGAGTCCATCGCCTGCCTGCTGGAACGCTATCGCTTCGAGAAC 
CTGCAACCGCAGCTCAAGGAAATCGGCGACCTCGAACGTATCCTCGCCCGCATCGGCCTGCGCAACGCCCGC 
CCTCGCGACCTGGCGCGCCTGCGCGACGCGCTGGCGGCGTTGCCGGACCTGCAGAACGCCATGACCGAACTG 
GAAGCGCCGCACCTGCAGGCGCTGGCCACCACCATCGGCACCTATCCCGAACTCGCCGAACTGCTGGCCAAG 
GCGATCATCGACAACCCGCCGGCGGTGATCCGCGACGGTGGCGTGATCAAGACCGGCTATGACGCCGAGCTG 
GACGAGCTGCAGGCGCTGAGCGAAAACGCCGGGCAATTCCTGATGGACCTGGAAGCGCGCGAGAAGGCCCGC 
ACCGGCCTGCCCAACCTGAAGGTCGGCTACAACCGCATCCATGGCTACTTCATCGAGCTGCCACGGGTGCAG 
GCCGAACAGGCGCCGGCCGACTACATCCGCCGGCAGACCCTGAAAGGCGCCGAGCGCTTCATCACGCCGGAA 
CTGAAGGCCTTCGAGGACAAGGCGCTGTCGGCCCAGAGCCGCGCCCTGGCCCGCGAGAAGGCACTCTACGAA 
GAGCTGCTGGAACGTCTGATCGGCCACCTCGCTCCGCTCCAGGACAGCGCCTCGGCGCTGGCGGAGCTGGAC 
GTGCTGGCGAACCTCGCCGAACGCGCGCTGAACCTCGACCTGAATCGCCCGCGGTTCGTCGAACACACCTGC 
CTGCACATCGAGCAGGGCCGCCATCCGGTGGTCGAGCAGGTGCTGGAGACGCCGTTCGTGGCCAACGACCTG 
GCGCTGGATGCCGACACCCGGATGCTGGTGATCACCGGTCCGAACATGGGCGGTAAATCCACCTACATGCGG 
CAAACCGCGCTGATCGTGCTGCTTGCGCATATCGGCAGCTTCGTTCCGGCTGCGCGCTGCGAGCTGTCCCTG 
GTGGACCGCATCTTCACCCGCATCGGCTCGTCCGACGACCTTGCCGGCGGCCGCTCGACCTTCATGGTGGAG 
ATGAGCGAAACCGCCAACATCCTGCACAACGCCACTGACAAGAGCCTGGTGCTGATGGATGAGGTCGGCCGC 
GGCACCAGCACCTTCGACGGCCTGTCGCTGGCCTGGGCAGCGGCCGAGGACCTGGCCCGGACTCGCGCCTTC 
ACCCTGTTCGCCACCCACTACTTCGAGCTGACCGTACTGCCGGAAAGCCAGCCCGCGGTAGCCAACGTGCAC 
CTGAACGCGACCGAGCACAATGAACGCATCGTGTTCCTGCACCATGTACTGCCGGGACCGGCGAGCCAGAGC 
TACGGCCTCGCGGTGGCCCAGTTGGCCGGCGTGCCGACTCCGGTAATCCAGCGCGCCCGCGAACACCTCAAG 
CGCCTGGAAACCACCAGCCTGCCGCACGAGATGCCGAGCCAGCAGAGCGGCAAGCCCGCCTCGCCGATGCAG 
AGCGACCTGTTCGCCAGCCTGCCGCACCCGGTGATCGATGAATTGTCGAGGATCAATCCCGACGATATCAGC 
CCGCGGCAAGCTCTCGATCTGTTATATGCATGGAAGATGCGGGTCTGA