ViewGene

Locus Name:
GeneID:
Location:

GeneID: 1468

TrivialName: PA14_53010

AnnotatorUID:

Modification Date/Time: 2006-04-06 13:01:23

LocusName: PA14_53010

PAO1 Ortholog Locus: PA0870

Sequence Length: 1200

Protein Length: 399

Start: 4699443

Stop: 4700642

Strand: +

Type:

ChromosomeID: 2

Status: ACTIVE

Frame Discrepancy: FALSE

Missense Discrepancy: FALSE

Comments:
Homology: gb|AAG04259.1|AE004522_1 (AE004522) aromatic amino acid aminotransferase [Pseudomonas aeruginosa PAO1] Identities = 398/399 (99%), Positives = 399/399 (100%)

Sequence:

ATGAGTCATTTCGCCAAGGTCGCCCGCGTACCGGGCGACCCGATCCTGGGCCTGCTCGACGCCTACCGCAAC
GATCCGCGCGCGGACAAGCTGGACCTCGGCGTCGGTGTCTACAAGGATGCCCAGGGCCTGACCCCGATCCTG
CGCTCGGTGAAACTCGCCGAGCAGCGCCTGGTCGAGCAGGAAACCACCAAGAGCTACGTCGGCGGCCACGGC
GATGCGCTGTTCGCCGCGCGCCTGGCGGAACTGGCGCTCGGCGCCGCCTCGCCGCTGTTGCTGGAGCAACGC
GCCGACGCCACCCAGACGCCCGGCGGCACCGGCGCCTTGCGCCTGGCCGGCGACTTCATCGCCCATTGCCTG
CCCGGCCGCGGCATCTGGCTGAGCGACCCGACCTGGCCGATCCACGAGACGCTGTTCGCCGCCGCCGGCCTG
AAGGTTTCCCACTACCCCTACGTCAGCGCCGACAACCGCCTGGATGTCGAGGCGATGCTTGCTGGCCTGGAG
CGCATTCCCCAGGGAGACGTGGTGCTGCTGCATGCCTGCTGCCACAACCCGACCGGTTTCGACCTGAGCCAC
GACGACTGGCGCAGGGTGCTCGACGTGGTGCGTCGTCGCGAGCTGCTGCCGCTGATCGACTTCGCCTACCAG
GGCTTCGGCGACGGTCTCGAGGAAGACGCCTGGGCGGTACGCCTGTTCGCCGGCGAACTGCCGGAGGTGCTG
GTCACCAGTTCCTGCTCGAAGAACTTCGGCCTGTACCGCGACCGCGTCGGCGCGCTGATCGTCTGCGCGCAG
AACGCCGAGAAGCTCACCGACGTGCGTAGCCAACTGGCCTTCCTCGCCCGCAACCTCTGGTCGACCCCGCCG
GCGCATGGCGCCGAGGTGGTCGCGGCGATCCTCGGCGACAGCGAGTTGAAGGGACTCTGGCAGGAAGAGGTC
GAAGGCATGCGCTCGCGCATCGCCAGCCTGCGCATCGGCCTGGTCGAAGCCCTGGCGCCGCACGGCCTGGCC
GAGCGCTTCGCCCATGTCGGCGCGCAACGCGGGATGTTTTCCTATACCGGACTGAGCCCGCAGCAGGTCGCT
CGGCTGCGCGACGAGCACGCCGTTTACCTGGTCTCCAGCGGCCGGGCCAACGTCGCCGGTCTCGACGCGCGC
CGCCTCGACCGCCTGGCGCAAGCCATCGCCCAGGTCTGCGCGGACTGA

Translation:

MSHFAKVARVPGDPILGLLDAYRNDPRADKLDLGVGVYKDAQGLTPILRSVKLAEQRLVEQETTKSYVGGHG
DALFAARLAELALGAASPLLLEQRADATQTPGGTGALRLAGDFIAHCLPGRGIWLSDPTWPIHETLFAAAGL
KVSHYPYVSADNRLDVEAMLAGLERIPQGDVVLLHACCHNPTGFDLSHDDWRRVLDVVRRRELLPLIDFAYQ
GFGDGLEEDAWAVRLFAGELPEVLVTSSCSKNFGLYRDRVGALIVCAQNAEKLTDVRSQLAFLARNLWSTPP
AHGAEVVAAILGDSELKGLWQEEVEGMRSRIASLRIGLVEALAPHGLAERFAHVGAQRGMFSYTGLSPQQVA
RLRDEHAVYLVSSGRANVAGLDARRLDRLAQAIAQVCAD*

AnnotationID:1477 GeneID:1468

AnnotatorUID: danlee

Modification Date/Time: 2004-05-03 12:12:39

Gene Name: phhC

Confidence Code: 1

GeneProduct: aromatic amino acid aminotransferase

Cell Localization: (1) Cytoplasmic

Synonyms: PA0870, tyrB, aspC�

Cell Localization Confidence Code: 1

MolecularFunction:

Functional Category: (2) Amino acid biosynthesis and metabolism

Alternate Gene Product Name:

Functional Category Confidence Code: 5

COGs: COG1448, KOG1411, KOG1412

Secondary Functional Category(ies):

EC Number:

Status: ACTIVE

Pathway:

Phenylalanine metabolism

Homology:

Structural Features:

COG1448, TyrB, Aspartate/tyrosine/aromatic aminotransferase.  Score: 570, 2e-163
KOG1411, Aspartate aminotransferase/Glutamic oxaloacetic transaminase AAT1/GOT2. Score: 352, 7e-98
KOG1412, Aspartate aminotransferase/Glutamic oxaloacetic transaminase AAT

Genomic Context:

The fourth gene of a 4-gene cluster encoding phenylalanine hydroxylase (PhhA),
carbinolamine dehydratase (PhhB), and aromatic aminotransferase (PhhC);
and in the upstream region, a divergently transcribed gene (phhR) encoding an activator protein.

Comment:

ReferenceID:2939

Author/Investigator(s): GenNotator

Title:

PubMed:

MedLine:

Source:

Reference Type: BLASTP

Data:

URL:

ReferenceID:20084

Author/Investigator(s): GenNotator

Title: CDD Search

PubMed:

MedLine:

Source:

Reference Type: RPSBLAST

Data:

URL:

ReferenceID:31994

Author/Investigator(s): Zhao G, Xia T, Song J, Jensen RA.

Title: Pseudomonas aeruginosa possesses homologues of mammalian phenylalanine hydroxylase and 4 alpha-carbinolamine dehydratase/DCoH as part of a three-component gene cluster.

PubMed: 94151331

MedLine:

Source:

Proc Natl Acad Sci U S A. 1994 Feb 15;91(4):1366-70.

Reference Type: Journal

Data:

URL:

ReferenceID:31995

Author/Investigator(s): Gu W, Song J, Bonner CA, Xie G, Jensen RA.

Title: PhhC is an essential aminotransferase for aromatic amino acid catabolism in Pseudomonas aeruginosa.

PubMed: 99061206

MedLine:

Source:

Microbiology. 1998 Nov;144 ( Pt 11):3127-34.

Reference Type: Journal

Data:

URL:

Homologs By Global Alignment

Gene ID:1468

Identity:

10%

20%

30%

40%

50%

60%

70%

80%

90%

100%

HomologID Accession Description Length PctIdentity PctSimilarity Gaps Score

2638 PA0870_tr translation of PA0870 399 99.74 99.99 0 2053.0

82922 gb|AAG04259.1|AE004522_1 (AE004522) aromatic amino acid aminotransferase [Pseudomonas aeruginosa PAO1] 399 99.74 99.99 0 2053.0

82923 gb|AAA25938.1| (M88627) phenylalanine hydroxylase [Pseudomonas aeruginosa] 399 99.24 99.49 0 2039.0

82930 gb|AAO10627.1|AE016804_137 (AE016804) Aspartate aminotransferase [Vibrio vulnificus CMCP6] 401 47.88 63.09 4 932.0

82931 dbj|BAC94860.1| (AP005338) aspartate aminotransferase [Vibrio vulnificus YJ016] 401 47.88 63.09 4 932.0

GC ORFID: `44470`	How Found: `BLASTX`
GC_TrimmedSeqID: `69`	Blast Result ID
Subject Sequence Name: `t_PA0870`	Glimmer Score:
Start: `254464`	Stop: `253265`
Length: `1200`
Start Codon: `ATG`	Truncated Start:
Stop Codon: `TGA`	Truncated Stop:
Homolog: `t_PA0870 translation of PA0870`	Homolog Bit Score `795.0`
Other Homologs: `t_PA3139 (ORF: BLASTX 345.0), psyr_15may02_Scaffold1_revised_gene459 (ORF: BLASTX 331.0), psyr_15may02_Scaffold6_revised_gene6482 (ORF: BLASTX 322.0), t_PA0870 (ORF: GLIMMER)`
GC ORF Sequence
ATGAGTCATTTCGCCAAGGTCGCCCGCGTACCGGGCGACCCGATCCTGGGCCTGCTCGACGCCTACCGCAAC GATCCGCGCGCGGACAAGCTGGACCTCGGCGTCGGTGTCTACAAGGATGCCCAGGGCCTGACCCCGATCCTG CGCTCGGTGAAACTCGCCGAGCAGCGCCTGGTCGAGCAGGAAACCACCAAGAGCTACGTCGGCGGCCACGGC GATGCGCTGTTCGCCGCGCGCCTGGCGGAACTGGCGCTCGGCGCCGCCTCGCCGCTGTTGCTGGAGCAACGC GCCGACGCCACCCAGACGCCCGGCGGCACCGGCGCCTTGCGCCTGGCCGGCGACTTCATCGCCCATTGCCTG CCCGGCCGCGGCATCTGGCTGAGCGACCCGACCTGGCCGATCCACGAGACGCTGTTCGCCGCCGCCGGCCTG AAGGTTTCCCACTACCCCTACGTCAGCGCCGACAACCGCCTGGATGTCGAGGCGATGCTTGCTGGCCTGGAG CGCATTCCCCAGGGAGACGTGGTGCTGCTGCATGCCTGCTGCCACAACCCGACCGGTTTCGACCTGAGCCAC GACGACTGGCGCAGGGTGCTCGACGTGGTGCGTCGTCGCGAGCTGCTGCCGCTGATCGACTTCGCCTACCAG GGCTTCGGCGACGGTCTCGAGGAAGACGCCTGGGCGGTACGCCTGTTCGCCGGCGAACTGCCGGAGGTGCTG GTCACCAGTTCCTGCTCGAAGAACTTCGGCCTGTACCGCGACCGCGTCGGCGCGCTGATCGTCTGCGCGCAG AACGCCGAGAAGCTCACCGACGTGCGTAGCCAACTGGCCTTCCTCGCCCGCAACCTCTGGTCGACCCCGCCG GCGCATGGCGCCGAGGTGGTCGCGGCGATCCTCGGCGACAGCGAGTTGAAGGGACTCTGGCAGGAAGAGGTC GAAGGCATGCGCTCGCGCATCGCCAGCCTGCGCATCGGCCTGGTCGAAGCCCTGGCGCCGCACGGCCTGGCC GAGCGCTTCGCCCATGTCGGCGCGCAACGCGGGATGTTTTCCTATACCGGACTGAGCCCGCAGCAGGTCGCT CGGCTGCGCGACGAGCACGCCGTTTACCTGGTCTCCAGCGGCCGGGCCAACGTCGCCGGTCTCGACGCGCGC CGCCTCGACCGCCTGGCGCAAGCCATCGCCCAGGTCTGCGCGGACTGA