ViewGene
Locus Name:
GeneID:
Location:

GeneID:1464
TrivialName: PA14_04110
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_04110
PAO1 Ortholog Locus:PA0316
Sequence Length:1230
Protein Length:409
Start: 370625
Stop: 369396
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG03705.1|AE004469_10 (AE004469) D-3-phosphoglycerate dehydrogenase [Pseudomonas aeruginosa PAO1]
Identities = 408/409 (99%), Positives = 409/409 (100%)
Sequence:
ATGAGCAAGACCTCTCTCGACAAGAGCAAGATCAAGTTCCTTCTTCTCGAAGGTGTCCACCAGAACGCCGTG
GACACGCTGAAGGCCGCCGGCTACACCAACATCGAGTACCTCAAGACCGCGCTTTCCGGCGACGAGCTGAAG
GAAAGGATCGCCGATGCGCACTTCATCGGCATTCGCTCCCGCACCCAGCTGACCGAAGAGGTCTTCGACTGC
GCGAAGAAACTGATCGCGGTCGGCTGCTTCTGCATCGGCACCAACCAGGTCGACCTCAACGCCGCTCGCGAA
CGCGGTATCGCCGTGTTCAACGCGCCCTACTCGAACACCCGCTCTGTGGCTGAGCTGGTACTGGCCGAGGCG
ATCCTGCTGCTGCGCGGCATCCCGGAAAAGAACGCTTCCTGCCATCGCGGCGGCTGGATCAAGAGCGCGGCG
AACTCCTTCGAGATCCGTGGCAAGAAGCTCGGCATCGTCGGCTACGGTTCGATCGGCACCCAGCTCTCGGTA
CTCGCCGAGGCCCTGGGCATGCAGGTGTTCTTCTACGACACCGTGACCAAGCTGCCGCTGGGCAACGCGGTG
CAGATCGGCAGCCTGCACGAGCTGCTGGGCATGTCCGACATCGTCTCGCTGCACGTTCCCGAACTGCCCTCC
ACCCAGTGGATGATCGGCGAGAAGGAAATCCGCGCGATGAAGAAGGGCGGCATCCTGATCAACGCCGCGCGC
GGCACCGTGGTCGAGCTGGACCACCTGGCCGCCGCGATCAAGGATGAGCACCTGATCGGCGCCGCCATCGAC
GTGTTCCCGGTCGAGCCGAAATCCAACGACGAGGAATTCGCCAGCCCGCTGCGTGGCCTGGACCGGGTGATC
CTGACCCCGCACATCGGCGGCTCCACCGCCGAGGCACAGGCCAACATCGGCCTGGAAGTGGCGGAGAAGCTG
GTCAAGTACAGCGACAACGGCACCTCGGTGTCCTCGGTCAACTTCCCCGAAGTGGCCCTGCCGTCGCACCCG
GGCAAGCACCGCCTGCTGCACATCCACGCCAACATCCCCGGCGTGATGAGCGAGATCAACAAGGTCTTCGCC
GACAACGGCATCAACGTTTCCGGCCAGTACCTGCAAACCAACGATAAGGTCGGCTACGTGGTGATCGACGTC
GACGCCGAGTACTCCGACCTGGCCCTGGAGAAACTCCAGCAGGTCAACGGCACCATCCGTAGCCGCGTGCTG
TTCTAA
Translation:
MSKTSLDKSKIKFLLLEGVHQNAVDTLKAAGYTNIEYLKTALSGDELKERIADAHFIGIRSRTQLTEEVFDC
AKKLIAVGCFCIGTNQVDLNAARERGIAVFNAPYSNTRSVAELVLAEAILLLRGIPEKNASCHRGGWIKSAA
NSFEIRGKKLGIVGYGSIGTQLSVLAEALGMQVFFYDTVTKLPLGNAVQIGSLHELLGMSDIVSLHVPELPS
TQWMIGEKEIRAMKKGGILINAARGTVVELDHLAAAIKDEHLIGAAIDVFPVEPKSNDEEFASPLRGLDRVI
LTPHIGGSTAEAQANIGLEVAEKLVKYSDNGTSVSSVNFPEVALPSHPGKHRLLHIHANIPGVMSEINKVFA
DNGINVSGQYLQTNDKVGYVVIDVDAEYSDLALEKLQQVNGTIRSRVLF*
AnnotationID:1473GeneID:1464
AnnotatorUID: diggins
Modification Date/Time: 2005-03-24 12:12:57
Gene Name:serA
Confidence Code:2
GeneProduct:D-3-phosphoglycerate dehydrogenase
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(2) Amino acid biosynthesis and metabolism
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:COG0111,COG1052
Secondary Functional Category(ies):energy metabolism
EC Number:1.1.1.95
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAN70720.1|AE016715_9 (AE016793) D-3-phosphoglycerate dehydrogenase [Pseudomonas putida
           KT2440]
          Length = 409

 Score =  735 bits (1898), Expect = 0.0
 Identities = 371/409 (90%), Positives = 393/409 (96%)

>gb|AAO58720.1| (AE016875) D-3-phosphoglycerate dehydrogenase [Pseudomonas syringae
           pv. tomato str. DC3000]
          Length = 409

 Score =  714 bits (1842), Expect = 0.0
 Identities = 363/409 (88%), Positives = 384/409 (93%)

>gb|AAN53938.1|AE015531_4 (AE015531) D-3-phosphoglycerate dehydrogenase [Shewanella
           oneidensis MR-1]
          Length = 409

 Score =  548 bits (1411), Expect = e-155
 Identities = 269/409 (65%), Positives = 329/409 (80%)






 
Structural Features:
COG0111, SerA, Phosphoglycerate dehydrogenase and related dehydrogenases 
[Amino acid transport and metabolism]
CD-Length = 324 residues, 100.0% aligned
Score =  314 bits (807), Expect = 9e-87
Identities = 142/332 (42%), Positives = 187/332 (56%), Gaps = 11/332 (3%)

COG1052, LdhA, Lactate dehydrogenase and related dehydrogenases 
[Energy production and conversion / Coenzyme metabolism]
CD-Length = 324 residues,  99.1% aligned
Score =  217 bits (553), Expect = 2e-57
Identities = 110/324 (33%), Positives = 155/324 (47%), Gaps = 10/324 (3%)






   
Genomic Context:






   
Comment:


















    ReferenceID:2931




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:20076




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:33661




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:1464_cdd.html


    		




 
URL:

















    ReferenceID:35425




  
Author/Investigator(s):
        Tobey KL, Grant GA.





  
Title:
        The nucleotide sequence of the serA gene of Escherichia coli and the amino acid sequence of the encoded protein, D-3-phosphoglycerate dehydrogenase.




  
PubMed:
        3017965


MedLine:
        




    
Source:
J Biol Chem. 1986 Sep 15;261(26):12179-83.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=3017965

















    ReferenceID:35426




  
Author/Investigator(s):
        Kawabata S, Terao Y, Hamada S.





  
Title:
        Molecular cloning, sequence and characterization of a novel streptococcal phosphoglycerate dehydrogenase gene.




  
PubMed:
        11155166


MedLine:
        




    
Source:
Oral Microbiol Immunol. 2000 Feb;15(1):58-62.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=11155166









           
Homologs By Global Alignment 

           

       



     
Gene ID:1464
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
2632
                           PA0316_tr
                           translation of PA0316
                           409
                           99.75
                           99.99
                           0
                           2059.0
     

     
     
82278
                           gb|AAG03705.1|AE004469_10
                           (AE004469) D-3-phosphoglycerate dehydrogenase [Pseudomonas aeruginosa PAO1]
                           409
                           99.75
                           99.99
                           0
                           2059.0
     

     
     
82279
                           gb|AAN70720.1|AE016715_9
                           (AE016793) D-3-phosphoglycerate dehydrogenase [Pseudomonas putida KT2440]
                           409
                           90.70
                           96.08
                           0
                           1898.0
     

     
     
82280
                           gb|AAO58720.1|
                           (AE016875) D-3-phosphoglycerate dehydrogenase [Pseudomonas syringae pv. tomato str. DC3000]
                           409
                           88.75
                           93.88
                           0
                           1842.0
     

     
     
82282
                           gb|AAC22124.1|
                           (U32729) D-3-phosphoglycerate dehydrogenase (serA) [Haemophilus influenzae Rd] [Haemophilus influenzae Rd KW20]
                           410
                           66.09
                           80.24
                           1
                           1410.0
     

     






      

          
GC ORFID: 44466How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA0316Glimmer Score: 

          
Start: 6171967Stop: 6170738

          
Length: 1230


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TAATruncated Stop: 

          
Homolog: t_PA0316 translation of PA0316Homolog Bit Score 797.0

          
Other Homologs:  psyr_15may02_Scaffold6_revised_gene6914 (ORF: BLASTX 718.0), t_PA0316 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGAGCAAGACCTCTCTCGACAAGAGCAAGATCAAGTTCCTTCTTCTCGAAGGTGTCCACCAGAACGCCGTG 
GACACGCTGAAGGCCGCCGGCTACACCAACATCGAGTACCTCAAGACCGCGCTTTCCGGCGACGAGCTGAAG 
GAAAGGATCGCCGATGCGCACTTCATCGGCATTCGCTCCCGCACCCAGCTGACCGAAGAGGTCTTCGACTGC 
GCGAAGAAACTGATCGCGGTCGGCTGCTTCTGCATCGGCACCAACCAGGTCGACCTCAACGCCGCTCGCGAA 
CGCGGTATCGCCGTGTTCAACGCGCCCTACTCGAACACCCGCTCTGTGGCTGAGCTGGTACTGGCCGAGGCG 
ATCCTGCTGCTGCGCGGCATCCCGGAAAAGAACGCTTCCTGCCATCGCGGCGGCTGGATCAAGAGCGCGGCG 
AACTCCTTCGAGATCCGTGGCAAGAAGCTCGGCATCGTCGGCTACGGTTCGATCGGCACCCAGCTCTCGGTA 
CTCGCCGAGGCCCTGGGCATGCAGGTGTTCTTCTACGACACCGTGACCAAGCTGCCGCTGGGCAACGCGGTG 
CAGATCGGCAGCCTGCACGAGCTGCTGGGCATGTCCGACATCGTCTCGCTGCACGTTCCCGAACTGCCCTCC 
ACCCAGTGGATGATCGGCGAGAAGGAAATCCGCGCGATGAAGAAGGGCGGCATCCTGATCAACGCCGCGCGC 
GGCACCGTGGTCGAGCTGGACCACCTGGCCGCCGCGATCAAGGATGAGCACCTGATCGGCGCCGCCATCGAC 
GTGTTCCCGGTCGAGCCGAAATCCAACGACGAGGAATTCGCCAGCCCGCTGCGTGGCCTGGACCGGGTGATC 
CTGACCCCGCACATCGGCGGCTCCACCGCCGAGGCACAGGCCAACATCGGCCTGGAAGTGGCGGAGAAGCTG 
GTCAAGTACAGCGACAACGGCACCTCGGTGTCCTCGGTCAACTTCCCCGAAGTGGCCCTGCCGTCGCACCCG 
GGCAAGCACCGCCTGCTGCACATCCACGCCAACATCCCCGGCGTGATGAGCGAGATCAACAAGGTCTTCGCC 
GACAACGGCATCAACGTTTCCGGCCAGTACCTGCAAACCAACGATAAGGTCGGCTACGTGGTGATCGACGTC 
GACGCCGAGTACTCCGACCTGGCCCTGGAGAAACTCCAGCAGGTCAACGGCACCATCCGTAGCCGCGTGCTG 
TTCTAA