ViewGene
Locus Name:
GeneID:
Location:

GeneID:1457
TrivialName: PA14_14730
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_14730
PAO1 Ortholog Locus:PA3814
Sequence Length:1215
Protein Length:404
Start: 1254357
Stop: 1255571
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG07201.1|AE004799_7 (AE004799) L-cysteine desulfurase (pyridoxal phosphate-dependent) [Pseudomonas aeruginosa PAO1]
Identities = 404/404 (100%), Positives = 404/404 (100%)
Sequence:
ATGAAATTGCCGATCTACCTCGATTACTCCGCGACCACTCCCGTCGATCCCCGCGTCGCCCAGAAAATGAGC
GAATGCCTGCTGATGGACGGCAACTTCGGCAACCCGGCTTCGCGTTCCCACGTGTTCGGCTGGAAAGCCGAG
GAGGCCGTGGAGAACGCCCGTCGCCAGGTGGCCGAACTGGTCAACGCCGATCCCCGCGAGATCGTCTGGACC
TCCGGCGCCACCGAGTCCGACAACCTGGCGATCAAGGGCGTCGCGCACTTCTACTCGGGCAAGGGCAAGCAC
ATCATCACCTCGAAGATCGAGCACAAGGCGGTCCTCGACACCTGCCGCCAGCTGGAGCGCGAAGGTTTCGAA
GTGACCTACCTGGAGCCGGGCGAAGATGGCCTGATCACTCCGGCACTGGTCGAGGCGGCGCTGCGTGACGAC
ACCATCCTGGTCTCGGTCATGCACGTGAACAACGAGATCGGCACCGTCAACGACATCGCCGCCATCGGCGAG
CTGACCCGCTCCCGCGGCGTGCTGTTCCACGTCGACGCCGCACAGTCGACCGGCAAGGTCGAGATCGACCTG
GACAAGCTCAAGGTCGACCTGATGTCCTTCTCCGCGCACAAGACCTACGGCCCGAAAGGCATTGGCGCGCTC
TACGTCCGGCGCAAGCCGCGCGTCCGCATCGAGGGCCAGATGCACGGCGGCGGCCATGAGCGCGGCATGCGT
TCCGGCACCCTGGCCACCCACCAGATCGTCGGCATGGGCGAGGCGTTCCGCATCGCCAAGGAAGAAATGGCC
CAGGAGAACGCCCGCGTGCTGGCGCTCCGCGACCGCTTCTTCGCGCAGATCGACGGCCTGGAAGAGCTCTAC
ATCAACGGCAGCATGACCTCCCGCGTCCCGCACAACCTCAACGTCAGCTTCAACTACGTCGAGGGCGAGTCG
CTGATCATGGCGCTCAAGGACCTCGCGGTCTCCTCCGGTTCGGCCTGTACCTCGGCGTCCCTGGAACCGTCC
TACGTATTGCGCGCCCTGGGCCGCAACGACGAACTGGCGCATAGCTCCATCCGCTTCACCTTCGGTCGCTTC
ACCACCGAGGAAGAGATCGACTATGCCGCGAAGAAGGTGGTCGAGGCCGTTTCCAAGCTGCGCGAACTGTCG
CCGCTGTGGGATATGTACAAAGAGGGCGTCGACCTGTCCCAGGTCGAATGGCAGGCGCACTGA
Translation:
MKLPIYLDYSATTPVDPRVAQKMSECLLMDGNFGNPASRSHVFGWKAEEAVENARRQVAELVNADPREIVWT
SGATESDNLAIKGVAHFYSGKGKHIITSKIEHKAVLDTCRQLEREGFEVTYLEPGEDGLITPALVEAALRDD
TILVSVMHVNNEIGTVNDIAAIGELTRSRGVLFHVDAAQSTGKVEIDLDKLKVDLMSFSAHKTYGPKGIGAL
YVRRKPRVRIEGQMHGGGHERGMRSGTLATHQIVGMGEAFRIAKEEMAQENARVLALRDRFFAQIDGLEELY
INGSMTSRVPHNLNVSFNYVEGESLIMALKDLAVSSGSACTSASLEPSYVLRALGRNDELAHSSIRFTFGRF
TTEEEIDYAAKKVVEAVSKLRELSPLWDMYKEGVDLSQVEWQAH*
AnnotationID:1466GeneID:1457
AnnotatorUID: diggins
Modification Date/Time: 2005-04-24 12:12:59
Gene Name:iscS
Confidence Code:2
GeneProduct:cysteine desulfurase
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(2) Amino acid biosynthesis and metabolism
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:COG1104,KOG1549
Secondary Functional Category(ies):
EC Number:2.8.1.7
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAO54944.1| (AE016860) cysteine desulfurase [Pseudomonas syringae pv. tomato
           str. DC3000]
          Length = 404

 Score =  721 bits (1861), Expect = 0.0
 Identities = 352/404 (87%), Positives = 385/404 (95%)

>gb|AAN81506.1|AE016764_188 (AE016764) Cysteine desulfurase [Escherichia coli CFT073]
          Length = 412

 Score =  617 bits (1591), Expect = e-176
 Identities = 306/404 (75%), Positives = 347/404 (85%)








 
Structural Features:
COG1104, NifS, Cysteine sulfinate desulfinase/cysteine desulfurase 
and related enzymes 
[Amino acid transport and metabolism]
CD-Length = 386 residues, 100.0% aligned
Score =  556 bits (1435), Expect = 3e-159
Identities = 232/391 (59%), Positives = 289/391 (73%), Gaps = 10/391 (2%)

KOG1549, Cysteine desulfurase NFS1 
[Amino acid transport and metabolism]
CD-Length = 428 residues,  90.0% aligned
Score =  416 bits (1070), Expect = 6e-117
Identities = 215/389 (55%), Positives = 276/389 (70%), Gaps = 7/389 (1%)







   
Genomic Context:






   
Comment:


















    ReferenceID:2917




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:20062




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:31742




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:1457_cdd.html


    		




 
URL:

















    ReferenceID:34301




  
Author/Investigator(s):
        Takahashi Y, Nakamura M.





  
Title:
        Functional assignment of the ORF2-iscS-iscU-iscA-hscB-hscA-fdx-ORF3 gene cluster involved in the assembly of Fe-S clusters in Escherichia coli.




  
PubMed:
        10544286


MedLine:
        




    
Source:
J Biochem (Tokyo). 1999 Nov;126(5):917-26.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=10544286









           
Homologs By Global Alignment 

           

       



     
Gene ID:1457
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
2625
                           PA3814_tr
                           translation of PA3814
                           404
                           99.99
                           99.99
                           0
                           2064.0
     

     
     
82038
                           gb|AAG07201.1|AE004799_7
                           (AE004799) L-cysteine desulfurase (pyridoxal phosphate-dependent) [Pseudomonas aeruginosa PAO1]
                           404
                           99.99
                           99.99
                           0
                           2064.0
     

     
     
82039
                           gb|AAC24472.1|
                           (AF010139) IscS [Azotobacter vinelandii]
                           404
                           88.86
                           94.80
                           0
                           1871.0
     

     
     
82040
                           gb|AAO54944.1|
                           (AE016860) cysteine desulfurase [Pseudomonas syringae pv. tomato str. DC3000]
                           404
                           87.12
                           95.29
                           0
                           1861.0
     

     
     
82041
                           gb|AAN66467.1|AE016276_2
                           (AE016777) cysteine desulfurase [Pseudomonas putida KT2440]
                           404
                           86.63
                           94.30
                           0
                           1859.0
     

     






      

          
GC ORFID: 44459How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA3814Glimmer Score: 

          
Start: 3699581Stop: 3698367

          
Length: 1215


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA3814 translation of PA3814Homolog Bit Score 799.0

          
Other Homologs:  psyr_15may02_Scaffold3_revised_gene4517 (ORF: BLASTX 726.0), t_PA2062 (ORF: BLASTX 397.0), psyr_15may02_Scaffold9_revised_gene8088 (ORF: BLASTX 377.0), t_PA3814 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGAAATTGCCGATCTACCTCGATTACTCCGCGACCACTCCCGTCGATCCCCGCGTCGCCCAGAAAATGAGC 
GAATGCCTGCTGATGGACGGCAACTTCGGCAACCCGGCTTCGCGTTCCCACGTGTTCGGCTGGAAAGCCGAG 
GAGGCCGTGGAGAACGCCCGTCGCCAGGTGGCCGAACTGGTCAACGCCGATCCCCGCGAGATCGTCTGGACC 
TCCGGCGCCACCGAGTCCGACAACCTGGCGATCAAGGGCGTCGCGCACTTCTACTCGGGCAAGGGCAAGCAC 
ATCATCACCTCGAAGATCGAGCACAAGGCGGTCCTCGACACCTGCCGCCAGCTGGAGCGCGAAGGTTTCGAA 
GTGACCTACCTGGAGCCGGGCGAAGATGGCCTGATCACTCCGGCACTGGTCGAGGCGGCGCTGCGTGACGAC 
ACCATCCTGGTCTCGGTCATGCACGTGAACAACGAGATCGGCACCGTCAACGACATCGCCGCCATCGGCGAG 
CTGACCCGCTCCCGCGGCGTGCTGTTCCACGTCGACGCCGCACAGTCGACCGGCAAGGTCGAGATCGACCTG 
GACAAGCTCAAGGTCGACCTGATGTCCTTCTCCGCGCACAAGACCTACGGCCCGAAAGGCATTGGCGCGCTC 
TACGTCCGGCGCAAGCCGCGCGTCCGCATCGAGGGCCAGATGCACGGCGGCGGCCATGAGCGCGGCATGCGT 
TCCGGCACCCTGGCCACCCACCAGATCGTCGGCATGGGCGAGGCGTTCCGCATCGCCAAGGAAGAAATGGCC 
CAGGAGAACGCCCGCGTGCTGGCGCTCCGCGACCGCTTCTTCGCGCAGATCGACGGCCTGGAAGAGCTCTAC 
ATCAACGGCAGCATGACCTCCCGCGTCCCGCACAACCTCAACGTCAGCTTCAACTACGTCGAGGGCGAGTCG 
CTGATCATGGCGCTCAAGGACCTCGCGGTCTCCTCCGGTTCGGCCTGTACCTCGGCGTCCCTGGAACCGTCC 
TACGTATTGCGCGCCCTGGGCCGCAACGACGAACTGGCGCATAGCTCCATCCGCTTCACCTTCGGTCGCTTC 
ACCACCGAGGAAGAGATCGACTATGCCGCGAAGAAGGTGGTCGAGGCCGTTTCCAAGCTGCGCGAACTGTCG 
CCGCTGTGGGATATGTACAAAGAGGGCGTCGACCTGTCCCAGGTCGAATGGCAGGCGCACTGA