ViewGene
Locus Name:
GeneID:
Location:

GeneID:1451
TrivialName: PA14_32400
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_32400
PAO1 Ortholog Locus:PA2493
Sequence Length:1245
Protein Length:414
Start: 2818608
Stop: 2817364
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG05881.1|AE004676_13 (AE004676) RND multidrug efflux membrane fusion protein MexE precursor [Pseudomonas aeruginosa PAO1]
Identities = 414/414 (100%), Positives = 414/414 (100%)
Sequence:
ATGGAACAGTCATCCCACTTCTCCTGGCGCTACCCCCTCGCACTCGCGGCCGTACTGGTCCTGAGCGCCTGC
GGCAAGGCCCCGGAAACCACCCAAGGCATGGCGGCGCCCAAGGTCAGCGTCGCCGAAGTCATCGAACAACCG
CTGAACGAGTGGGACGAATTCACCGGCCGCCTGGAGGCCCCGGAGTCGGTGGAGCTGCGCCCGCGGGTATCG
GGCTACATCGACCGCGTGGCCTTCCATGAAGGCGCCCTGGTGAAGAAAGGCGACCTGCTGTTCCAGATCGAC
CCGCGCCCGTTCGAGGCCGAGGTCAAGCGCCTCGAAGCCCAGCTGCAACAGGCCCGCGCGGCCCAGGCGCGG
AGCGTCAACGAAGCCCAGCGCGGCGAACGCCTGCGCGCCAGCAACGCGATCTCCGCGGAACTCGCCGACGCC
CGCACCACCGCCGCCCAGGAAGCCAAGGCGGCGGTCGCCGCGACCCAGGCGCAACTGGACGCGGCACGCCTG
AACCTGAGCTTCACCCGGATCACCGCGCCGATCGACGGTCGCGTCAGCCGCGCCGAGGTCACCGCCGGCAAC
CTGGTCAACTCCGGGGAGACCCTGCTCACCACCCTGGTCAGCACCGACAAGGTCTACGCCTACTTCGACGCC
GACGAGCGCGTGTTCCTCAAGTACGTCGAGCTGGCCCGCCAGGCCGGTCGCGACACGCGCAGCGAGAGCCCG
GTGTACCTCGGCCTGAGCAGCGAGGACGGCAACCCGCACCTGGGCCGGCTGGACTTCCTCGACAACCAGGTC
AACCCGCGTACCGGCACCATCCGCGGCCGCGCCGTGTTCGACAACGCCAAGGGCGAGTTCACCCCGGGCCTC
TACGTGCGCCTGAAGCTGGTCGGCAGCAAGACCTACGCCGCCACCCTGATCAAGGACGAAGCGGTCGGCACC
GACCTGGGCAAGAAGTTCGTGCTGGTCCTGGATGGCGACAACAAGACCGTCTACCGCACTGTCGAGATGGGA
CCGAAGCTGGAGGGCCTGCGCATCGTCCGCAGCGGCCTGAGCAAGGGCGACCGGATCGTCGTGAATGGCCTG
CAGCGGGTCCGCCCGGGCATGCAGGTGGATCCGCAGAAGGTCGAGATGGCCAGCGCCGACACCCTGGCCACC
CTCGCGCGCCTGCGGCAGTCGGTCGGCGACAGCGAACCACCGAAGGTGGCGGCGTCCAAGGACAACGCCACT
CGCAACGAGCCGCGCGGCTGA
Translation:
MEQSSHFSWRYPLALAAVLVLSACGKAPETTQGMAAPKVSVAEVIEQPLNEWDEFTGRLEAPESVELRPRVS
GYIDRVAFHEGALVKKGDLLFQIDPRPFEAEVKRLEAQLQQARAAQARSVNEAQRGERLRASNAISAELADA
RTTAAQEAKAAVAATQAQLDAARLNLSFTRITAPIDGRVSRAEVTAGNLVNSGETLLTTLVSTDKVYAYFDA
DERVFLKYVELARQAGRDTRSESPVYLGLSSEDGNPHLGRLDFLDNQVNPRTGTIRGRAVFDNAKGEFTPGL
YVRLKLVGSKTYAATLIKDEAVGTDLGKKFVLVLDGDNKTVYRTVEMGPKLEGLRIVRSGLSKGDRIVVNGL
QRVRPGMQVDPQKVEMASADTLATLARLRQSVGDSEPPKVAASKDNATRNEPRG*
AnnotationID:1460GeneID:1451
AnnotatorUID: diggins
Modification Date/Time: 2005-03-06 15:03:17
Gene Name:mexE
Confidence Code:1
GeneProduct:RND multidrug efflux membrane fusion protein MexE precursor
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(3) Antibiotic resistance and susceptibility
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:COG0845,pfam00529
Secondary Functional Category(ies):transport of small molecules
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAN69027.1|AE016535_1 (AE016786) multidrug efflux RND membrane fusion protein MexE
           [Pseudomonas putida KT2440]
          Length = 413

 Score =  560 bits (1443), Expect = e-159
 Identities = 295/416 (70%), Positives = 341/416 (81%), Gaps = 5/416 (1%)

>gb|AAO56586.1| (AE016867) multidrug efflux RND membrane fusion protein MexE
           [Pseudomonas syringae pv. tomato str. DC3000]
          Length = 414

 Score =  551 bits (1419), Expect = e-156
 Identities = 287/407 (70%), Positives = 338/407 (83%), Gaps = 5/407 (1%)

>gb|AAN56486.1|AE015785_9 (AE015785) RND multidrug efflux membrane fusion protein MexE
           [Shewanella oneidensis MR-1]
          Length = 476

 Score =  367 bits (942), Expect = e-101
 Identities = 198/377 (52%), Positives = 261/377 (69%), Gaps = 5/377 (1%)








 
Structural Features:
COG0845, AcrA, Membrane-fusion protein [Cell envelope biogenesis, outer membrane]
CD-Length = 372 residues,  95.2% aligned
Score =  139 bits (351), Expect = 4e-34
Identities = 123/364 (33%), Positives = 176/364 (48%), Gaps = 16/364 (4%)

pfam00529, HlyD, HlyD family secretion protein.
CD-Length = 309 residues, 100.0% aligned
Score =  136 bits (343), Expect = 5e-33
Identities = 87/310 (28%), Positives = 133/310 (42%), Gaps = 16/310 (5%)






   
Genomic Context:






   
Comment:


















    ReferenceID:2905




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:20049




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:33035




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:1451_cdd.html


    		




 
URL:

















    ReferenceID:34497




  
Author/Investigator(s):
        Poole K, Srikumar R.





  
Title:
        Multidrug efflux in Pseudomonas aeruginosa: components, mechanisms and clinical significance.




  
PubMed:
        11895293


MedLine:
        




    
Source:
Curr Top Med Chem. 2001 May;1(1):59-71.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=11895293

















    ReferenceID:34498




  
Author/Investigator(s):
        Aeschlimann JR.





  
Title:
        The role of multidrug efflux pumps in the antibiotic resistance of Pseudomonas aeruginosa and other gram-negative bacteria. Insights from the Society of Infectious Diseases Pharmacists.




  
PubMed:
        12885104


MedLine:
        




    
Source:
Pharmacotherapy. 2003 Jul;23(7):916-24.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=12885104









           
Homologs By Global Alignment 

           

       



     
Gene ID:1451
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
2615
                           PA2493_tr
                           translation of PA2493
                           414
                           99.99
                           99.99
                           0
                           2065.0
     

     
     
81905
                           gb|AAG05881.1|AE004676_13
                           (AE004676) RND multidrug efflux membrane fusion protein MexE precursor [Pseudomonas aeruginosa PAO1]
                           414
                           99.99
                           99.99
                           0
                           2065.0
     

     
     
81906
                           emb|CAA67866.1|
                           (X99514) hypothetical protein [Pseudomonas aeruginosa]
                           414
                           99.03
                           99.03
                           0
                           2042.0
     

     
     
81907
                           gb|AAN69027.1|AE016535_1
                           (AE016786) multidrug efflux RND membrane fusion protein MexE [Pseudomonas putida KT2440]
                           416
                           70.91
                           81.97
                           5
                           1451.0
     

     
     
81908
                           gb|AAO56586.1|
                           (AE016867) multidrug efflux RND membrane fusion protein MexE [Pseudomonas syringae pv. tomato str. DC3000]
                           420
                           69.76
                           81.90
                           12
                           1427.0
     

     






      

          
GC ORFID: 44453How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA2493Glimmer Score: 

          
Start: 2135316Stop: 2136560

          
Length: 1245


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA2493 translation of PA2493Homolog Bit Score 800.0

          
Other Homologs:  t_PA2493 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGGAACAGTCATCCCACTTCTCCTGGCGCTACCCCCTCGCACTCGCGGCCGTACTGGTCCTGAGCGCCTGC 
GGCAAGGCCCCGGAAACCACCCAAGGCATGGCGGCGCCCAAGGTCAGCGTCGCCGAAGTCATCGAACAACCG 
CTGAACGAGTGGGACGAATTCACCGGCCGCCTGGAGGCCCCGGAGTCGGTGGAGCTGCGCCCGCGGGTATCG 
GGCTACATCGACCGCGTGGCCTTCCATGAAGGCGCCCTGGTGAAGAAAGGCGACCTGCTGTTCCAGATCGAC 
CCGCGCCCGTTCGAGGCCGAGGTCAAGCGCCTCGAAGCCCAGCTGCAACAGGCCCGCGCGGCCCAGGCGCGG 
AGCGTCAACGAAGCCCAGCGCGGCGAACGCCTGCGCGCCAGCAACGCGATCTCCGCGGAACTCGCCGACGCC 
CGCACCACCGCCGCCCAGGAAGCCAAGGCGGCGGTCGCCGCGACCCAGGCGCAACTGGACGCGGCACGCCTG 
AACCTGAGCTTCACCCGGATCACCGCGCCGATCGACGGTCGCGTCAGCCGCGCCGAGGTCACCGCCGGCAAC 
CTGGTCAACTCCGGGGAGACCCTGCTCACCACCCTGGTCAGCACCGACAAGGTCTACGCCTACTTCGACGCC 
GACGAGCGCGTGTTCCTCAAGTACGTCGAGCTGGCCCGCCAGGCCGGTCGCGACACGCGCAGCGAGAGCCCG 
GTGTACCTCGGCCTGAGCAGCGAGGACGGCAACCCGCACCTGGGCCGGCTGGACTTCCTCGACAACCAGGTC 
AACCCGCGTACCGGCACCATCCGCGGCCGCGCCGTGTTCGACAACGCCAAGGGCGAGTTCACCCCGGGCCTC 
TACGTGCGCCTGAAGCTGGTCGGCAGCAAGACCTACGCCGCCACCCTGATCAAGGACGAAGCGGTCGGCACC 
GACCTGGGCAAGAAGTTCGTGCTGGTCCTGGATGGCGACAACAAGACCGTCTACCGCACTGTCGAGATGGGA 
CCGAAGCTGGAGGGCCTGCGCATCGTCCGCAGCGGCCTGAGCAAGGGCGACCGGATCGTCGTGAATGGCCTG 
CAGCGGGTCCGCCCGGGCATGCAGGTGGATCCGCAGAAGGTCGAGATGGCCAGCGCCGACACCCTGGCCACC 
CTCGCGCGCCTGCGGCAGTCGGTCGGCGACAGCGAACCACCGAAGGTGGCGGCGTCCAAGGACAACGCCACT 
CGCAACGAGCCGCGCGGCTGA