ViewGene
Locus Name:
GeneID:
Location:

GeneID:1439
TrivialName: PA14_25430
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_25430
PAO1 Ortholog Locus:PA2988
Sequence Length:1251
Protein Length:416
Start: 2222289
Stop: 2223539
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG06376.1|AE004724_5 (AE004724) conserved hypothetical protein [Pseudomonas aeruginosa PAO1]
Identities = 416/416 (100%), Positives = 416/416 (100%)
Sequence:
ATGTTCAGACCCCTGTCCGTTTTCATCGGCACGCGCTATACGCGGGCCAAACGTCGCAGCCATTTCGTCTCC
TTCATTTCGCTGACCTCGATGATCGGCCTGGCACTGGGCGTGCTGGTGATGATCGTGGTGCTCTCGGTGATG
AACGGCTTCGATCGCGAAATGCGTACCCGCATCCTCGGCATGGTGCCGCAGGCCACCGTCGAGTCGTACCAG
CCCATCGATGACTGGCGCGCCCTGGCCGAGAAGGTCAAGGCCCACGAGCATGTCACCGCGGTAGCGCCGTTC
ACCCAGATGCAGGGCATGCTCAGCGCCAATGGCGCGGTGCAGACGGTGATGGTCGATGCGGTCGATCCGCTG
GAAGAGGCCAAGGTCTCGATCATTCCCGATTTCTTCAAGCAGGGCTCGCTGGCGGAACTCAAGGCCGGCGGC
TTCGGCATCGTCATCGGCCAACTGGCGGCGCAGAAGCTTGGCGTCGGCATTGGCGACAAGGTCACCCTGATC
CTCCCCGAGGTGGCGGTCACCCCGGCCGGGGTATTCCCGCGGATGAAGCGTTTCACCGTGGTCGGCACCTTC
CGCGTCGGCGCCGGCGAACTGGATGGCGGCCTGTCGCTGATCCACCTGGAAGACGCCGCGCGCCTGCAGCGC
TGGAAGACCAACCAGGTACAGGGGCTGCGCCTGAAGCTCGACGACCTGTTCCAGGCGCCGCGGGTGGCCTGG
GAGATCGCCCGGACCCTGACCGACAATGACTTCTACGCCCGCGACTGGACGCGCAGCCACGGCAACCTGTAC
CAGGCCATCCGCATGGAAAAGACCATGATCGGCCTGTTGCTGCTGCTGATCGTCGCGGTCGCCGCGTTCAAC
ATCATTTCCACCCTGGTGATGGTGGTCACCGACAAGAAGTCGGACATCGCCATCCTTCGTACCCTCGGCGCC
ACGCCGGGGCAGATCATGGCTACCTTCATGGTCCAGGGTACGGTGATCGGGGTGATCGGCACGCTGGTCGGC
GGGGTCCTCGGGGTGGTCGCGGCACTCAACGTCAGCGCCTGGATTTCCGCCCTGGAAAAGCTGCTGGGCCAC
CAGTTCCTCGCGTCCGACGTGTACTTCATCGATTACCTGCCGTCGCAACTGATGCTCGACGATGTCGTGCTG
GTCTGCGGCGCGGCCCTGGTCCTCAGCTTCTTCGCCACTTTGTATCCAGCCTGGCGTGCGGCTCGCACCCAG
CCTGCGGAGGCTTTGCGTTATGAATGA
Translation:
MFRPLSVFIGTRYTRAKRRSHFVSFISLTSMIGLALGVLVMIVVLSVMNGFDREMRTRILGMVPQATVESYQ
PIDDWRALAEKVKAHEHVTAVAPFTQMQGMLSANGAVQTVMVDAVDPLEEAKVSIIPDFFKQGSLAELKAGG
FGIVIGQLAAQKLGVGIGDKVTLILPEVAVTPAGVFPRMKRFTVVGTFRVGAGELDGGLSLIHLEDAARLQR
WKTNQVQGLRLKLDDLFQAPRVAWEIARTLTDNDFYARDWTRSHGNLYQAIRMEKTMIGLLLLLIVAVAAFN
IISTLVMVVTDKKSDIAILRTLGATPGQIMATFMVQGTVIGVIGTLVGGVLGVVAALNVSAWISALEKLLGH
QFLASDVYFIDYLPSQLMLDDVVLVCGAALVLSFFATLYPAWRAARTQPAEALRYE*
AnnotationID:1448GeneID:1439
AnnotatorUID: diggins
Modification Date/Time: 2005-05-10 07:07:30
Gene Name:
Confidence Code:3
GeneProduct:putative lipoprotein releasing system, permease protein
Cell Localization:(4) Inner membrane protein
Synonyms:lolC
Cell Localization Confidence Code:3
MolecularFunction:
Functional Category:(27) Transport of small molecules
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:COG4591
Secondary Functional Category(ies):membrane protein
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAN67767.1|AE016408_5 (AE016782) lipoprotein releasing system, permease protein
           [Pseudomonas putida KT2440]
          Length = 416

 Score =  619 bits (1597), Expect = e-177
 Identities = 312/416 (75%), Positives = 358/416 (86%)

>gb|AAO55626.1| (AE016863) lipoprotein releasing system transmembrane protein LolC
           [Pseudomonas syringae pv. tomato str. DC3000]
          Length = 416

 Score =  617 bits (1592), Expect = e-176
 Identities = 315/416 (75%), Positives = 352/416 (84%)

>gb|AAM41411.1| (AE012319) lipoprotein releasing system transmembrane protein
           [Xanthomonas campestris pv. campestris str. ATCC 33913]
          Length = 416

 Score =  376 bits (966), Expect = e-103
 Identities = 198/417 (47%), Positives = 282/417 (67%), Gaps = 5/417 (1%)






 
Structural Features:
COG4591, LolE, ABC-type transport system, involved in lipoprotein release, 
permease component 
[Cell envelope biogenesis, outer membrane]
CD-Length = 408 residues, 100.0% aligned
Score =  426 bits (1098), Expect = 2e-120
Identities = 204/417 (48%), Positives = 269/417 (64%), Gaps = 10/417 (2%)







   
Genomic Context:






   
Comment:


















    ReferenceID:2881




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:20025




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:36066




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:1439_cdd.html


    		




 
URL:

















    ReferenceID:38125




  
Author/Investigator(s):
        Narita S, Tanaka K, Matsuyama S, Tokuda H.





  
Title:
        Disruption of lolCDE, encoding an ATP-binding cassette transporter, is lethal for Escherichia coli and prevents release of lipoproteins from the inner membrane.




  
PubMed:
        11844772


MedLine:
        




    
Source:
J Bacteriol. 2002 Mar;184(5):1417-22.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=11844772

















    ReferenceID:38128




  
Author/Investigator(s):
        Yakushi T, Masuda K, Narita S, Matsuyama S, Tokuda H.





  
Title:
        A new ABC transporter mediating the detachment of lipid-modified proteins from membranes.




  
PubMed:
        10783239


MedLine:
        




    
Source:
Nat Cell Biol. 2000 Apr;2(4):212-8.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=10783239









           
Homologs By Global Alignment 

           

       



     
Gene ID:1439
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
2601
                           PA2988_tr
                           translation of PA2988
                           416
                           99.99
                           99.99
                           0
                           2077.0
     

     
     
81658
                           gb|AAG06376.1|AE004724_5
                           (AE004724) conserved hypothetical protein [Pseudomonas aeruginosa PAO1]
                           416
                           99.99
                           99.99
                           0
                           2077.0
     

     
     
81660
                           gb|AAO55626.1|
                           (AE016863) lipoprotein releasing system transmembrane protein LolC [Pseudomonas syringae pv. tomato str. DC3000]
                           416
                           75.72
                           84.61
                           0
                           1592.0
     

     
     
81659
                           gb|AAN67767.1|AE016408_5
                           (AE016782) lipoprotein releasing system, permease protein [Pseudomonas putida KT2440]
                           416
                           75.00
                           86.05
                           0
                           1597.0
     

     
     
81664
                           gb|AAG34708.1|AF320001_1
                           (AF320001) putative integral membrane protein [Acinetobacter sp. BD413]
                           417
                           60.19
                           74.10
                           7
                           1216.0
     

     






      

          
GC ORFID: 44441How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA2988Glimmer Score: 

          
Start: 2731636Stop: 2730386

          
Length: 1251


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA2988 translation of PA2988Homolog Bit Score 804.0

          
Other Homologs:  psyr_15may02_Scaffold1_revised_gene898 (ORF: BLASTX 626.0), t_PA2986 (ORF: BLASTX 521.0), psyr_15may02_Scaffold1_revised_gene900 (ORF: BLASTX 503.0), t_PA2988 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGTTCAGACCCCTGTCCGTTTTCATCGGCACGCGCTATACGCGGGCCAAACGTCGCAGCCATTTCGTCTCC 
TTCATTTCGCTGACCTCGATGATCGGCCTGGCACTGGGCGTGCTGGTGATGATCGTGGTGCTCTCGGTGATG 
AACGGCTTCGATCGCGAAATGCGTACCCGCATCCTCGGCATGGTGCCGCAGGCCACCGTCGAGTCGTACCAG 
CCCATCGATGACTGGCGCGCCCTGGCCGAGAAGGTCAAGGCCCACGAGCATGTCACCGCGGTAGCGCCGTTC 
ACCCAGATGCAGGGCATGCTCAGCGCCAATGGCGCGGTGCAGACGGTGATGGTCGATGCGGTCGATCCGCTG 
GAAGAGGCCAAGGTCTCGATCATTCCCGATTTCTTCAAGCAGGGCTCGCTGGCGGAACTCAAGGCCGGCGGC 
TTCGGCATCGTCATCGGCCAACTGGCGGCGCAGAAGCTTGGCGTCGGCATTGGCGACAAGGTCACCCTGATC 
CTCCCCGAGGTGGCGGTCACCCCGGCCGGGGTATTCCCGCGGATGAAGCGTTTCACCGTGGTCGGCACCTTC 
CGCGTCGGCGCCGGCGAACTGGATGGCGGCCTGTCGCTGATCCACCTGGAAGACGCCGCGCGCCTGCAGCGC 
TGGAAGACCAACCAGGTACAGGGGCTGCGCCTGAAGCTCGACGACCTGTTCCAGGCGCCGCGGGTGGCCTGG 
GAGATCGCCCGGACCCTGACCGACAATGACTTCTACGCCCGCGACTGGACGCGCAGCCACGGCAACCTGTAC 
CAGGCCATCCGCATGGAAAAGACCATGATCGGCCTGTTGCTGCTGCTGATCGTCGCGGTCGCCGCGTTCAAC 
ATCATTTCCACCCTGGTGATGGTGGTCACCGACAAGAAGTCGGACATCGCCATCCTTCGTACCCTCGGCGCC 
ACGCCGGGGCAGATCATGGCTACCTTCATGGTCCAGGGTACGGTGATCGGGGTGATCGGCACGCTGGTCGGC 
GGGGTCCTCGGGGTGGTCGCGGCACTCAACGTCAGCGCCTGGATTTCCGCCCTGGAAAAGCTGCTGGGCCAC 
CAGTTCCTCGCGTCCGACGTGTACTTCATCGATTACCTGCCGTCGCAACTGATGCTCGACGATGTCGTGCTG 
GTCTGCGGCGCGGCCCTGGTCCTCAGCTTCTTCGCCACTTTGTATCCAGCCTGGCGTGCGGCTCGCACCCAG 
CCTGCGGAGGCTTTGCGTTATGAATGA