ViewGene
Locus Name:
GeneID:
Location:

GeneID:1437
TrivialName: PA14_61710
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_61710
PAO1 Ortholog Locus:PA4666
Sequence Length:1269
Protein Length:422
Start: 5506796
Stop: 5505528
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG08053.1|AE004880_9 (AE004880) glutamyl-tRNA reductase [Pseudomonas aeruginosa PAO1]
Identities = 422/422 (100%), Positives = 422/422 (100%)
Sequence:
ATGGCCTTCATTGCCCTCGGCATCAATCACAAGACCGCCTCGGTGGCTGTCCGCGAGCGCGTGGCTTTCACT
CCGGAACAGATGGTCGAGGCGCTGCAGCAGCTCTGCCGCCTGACCACCAGCCGCGAGGCGGCCATCCTGTCG
ACCTGCAACCGGAGCGAACTCTACCTGGAGATCGACCATCCGACCGCGGACGACGTGCTCGCCTGGCTGGCG
GACTACCATCGTCTGACCCTCGACGAGTTGCGCGCCTGCGCCTATGTGCACCAGGACGAAGACGCGGTGCGC
CACATGATGCGCGTGGCCTCCGGCCTCGACTCGATGGTCCTCGGCGAGCCGCAGATCCTCGGCCAGATGAAG
TCGGCCTACGCCGTCGCGCGCGAGGCCGGCACCGTCGGTCCGCTGCTAGGCCGCCTGTTCCAGGCCACCTTC
AGTACGGCGAAGACCGTCCGCACCGATACCGCCATCGGCGAGAACCCGGTGTCCGTGGCGTTCGCCGCGGTG
AGCCTGGCCAAGCAGATCTTCAGCGACCTGCACCGCAGCCAGGCGCTGCTGATCGGCGCCGGCGAGACCATC
ACCCTGGTCGCGCGCCACCTGTTCGAGCAAGGCGTGAAGCGCATCGTGGTGGCCAACCGCACCCTGGAGCGC
GCCAGCCTGCTCGCCGAGCAGTTCGGCGCCCATGCGGTGCTGCTTTCGGAGATACCCGAGGAGTTGGCCAAC
AGCGATATCGTCATCAGCTCCACTGCTAGCCAGTTGCCGATCCTCGGCAAGGGCGCCGTGGAGCGCGCGCTG
AAGCAGCGCAAGCACAAGCCGATGTTCATGGTCGACATCGCCGTGCCGCGCGACATCGAGCCGGAAGTCGGC
GAACTGGACGACGTCTACCTCTATAGCGTCGACGACCTCCACGAAGTAGTGGCGGAGAATCTCAAGAGCCGC
CAGGGCGCTGCCCAGGCCGCCGAGGAACTGGTCGGCAGCGGCGTCGCCGAGTTCATGCAGCGCCTGCGCGAA
CTGGCTGCGGTGGACGTGCTGCGCGCCTATCGGCAGCAGGCCGAGCGCCTGCGCGATGAAGAACTGGGCAAG
GCCCAGCGGCAACTGGCCAACGGTGCCGACCCGGCCGAGGTGATGGCCCAGTTGGCCCGCGGCCTGACCAAC
AAACTGCTGCACGCGCCCAGCGTGCAGATGAAGAAAATGTCCGCCGAGGGCCGCATCGACGCGCTGGCCCTG
GCCCAGGAGCTGTTCGCCCTCGACGAGGGCGCGCCGCGCCACTGA
Translation:
MAFIALGINHKTASVAVRERVAFTPEQMVEALQQLCRLTTSREAAILSTCNRSELYLEIDHPTADDVLAWLA
DYHRLTLDELRACAYVHQDEDAVRHMMRVASGLDSMVLGEPQILGQMKSAYAVAREAGTVGPLLGRLFQATF
STAKTVRTDTAIGENPVSVAFAAVSLAKQIFSDLHRSQALLIGAGETITLVARHLFEQGVKRIVVANRTLER
ASLLAEQFGAHAVLLSEIPEELANSDIVISSTASQLPILGKGAVERALKQRKHKPMFMVDIAVPRDIEPEVG
ELDDVYLYSVDDLHEVVAENLKSRQGAAQAAEELVGSGVAEFMQRLRELAAVDVLRAYRQQAERLRDEELGK
AQRQLANGADPAEVMAQLARGLTNKLLHAPSVQMKKMSAEGRIDALALAQELFALDEGAPRH*
AnnotationID:1446GeneID:1437
AnnotatorUID: diggins
Modification Date/Time: 2005-04-26 20:08:05
Gene Name:hemA
Confidence Code:1
GeneProduct:glutamyl-tRNA reductase
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(4) Biosynthesis of cofactors, prosthetic groups and carriers
Alternate Gene Product Name:delta-aminolevulinic synthase
Functional Category Confidence Code:5
COGs:COG0373,pfam05201,pfam05200
Secondary Functional Category(ies):
EC Number:1.2.1.70
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAN66357.1|AE016264_1 (AE016776) glutamyl-tRNA reductase [Pseudomonas putida KT2440]
          Length = 425

 Score =  699 bits (1805), Expect = 0.0
 Identities = 363/419 (86%), Positives = 396/419 (94%)

>gb|AAO54637.1| (AE016859) glutamyl-tRNA reductase [Pseudomonas syringae pv. tomato
           str. DC3000]
          Length = 425

 Score =  686 bits (1771), Expect = 0.0
 Identities = 358/419 (85%), Positives = 387/419 (92%)

>gb|AAN56811.1|AE015816_8 (AE015816) glutamyl-tRNA reductase [Shewanella oneidensis MR-1]
          Length = 416

 Score =  429 bits (1103), Expect = e-119
 Identities = 222/417 (53%), Positives = 301/417 (72%), Gaps = 2/417 (0%)






 
Structural Features:
COG0373, HemA, Glutamyl-tRNA reductase [Coenzyme metabolism]
CD-Length = 414 residues, 100.0% aligned
Score =  483 bits (1246), Expect = 1e-137
Identities = 218/418 (52%), Positives = 301/418 (72%), Gaps = 4/418 (0%)

pfam05201, GlutR_N, Glutamyl-tRNAGlu reductase, N-terminal domain.
CD-Length = 150 residues, 100.0% aligned
Score =  207 bits (528), Expect = 2e-54
Identities = 86/150 (57%), Positives = 110/150 (73%)

pfam05200, GlutR_NAD_bind, Glutamyl-tRNAGlu reductase, NAD(P) binding domain. 
CD-Length = 155 residues, 100.0% aligned
Score =  201 bits (513), Expect = 1e-52
Identities = 91/155 (58%), Positives = 118/155 (76%)






   
Genomic Context:






   
Comment:


















    ReferenceID:2877




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:20021




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:36552




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:1437_cdd.html


    		




 
URL:

















    ReferenceID:37765




  
Author/Investigator(s):
        Hungerer C, Troup B, Romling U, Jahn D.





  
Title:
        Regulation of the hemA gene during 5-aminolevulinic acid formation in Pseudomonas aeruginosa.




  
PubMed:
        7883699


MedLine:
        




    
Source:
J Bacteriol. 1995 Mar;177(6):1435-43.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=7883699

















    ReferenceID:37766




  
Author/Investigator(s):
        Verkamp E, Chelm BK.





  
Title:
        Isolation, nucleotide sequence, and preliminary characterization of the Escherichia coli K-12 hemA gene.




  
PubMed:
        2548996


MedLine:
        




    
Source:
J Bacteriol. 1989 Sep;171(9):4728-35.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=2548996

















    ReferenceID:37767




  
Author/Investigator(s):
        Elliott T.





  
Title:
        Cloning, genetic characterization, and nucleotide sequence of the hemA-prfA operon of Salmonella typhimurium.




  
PubMed:
        2544564


MedLine:
        




    
Source:
J Bacteriol. 1989 Jul;171(7):3948-60.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=2544564









           
Homologs By Global Alignment 

           

       



     
Gene ID:1437
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
2598
                           PA4666_tr
                           translation of PA4666
                           422
                           99.99
                           99.99
                           0
                           2079.0
     

     
     
81571
                           gb|AAG08053.1|AE004880_9
                           (AE004880) glutamyl-tRNA reductase [Pseudomonas aeruginosa PAO1]
                           422
                           99.99
                           99.99
                           0
                           2079.0
     

     
     
81572
                           emb|CAA57574.1|
                           (X82071) glutamyl-tRNA reductase [Pseudomonas aeruginosa]
                           422
                           90.28
                           91.23
                           34
                           1884.0
     

     
     
81573
                           gb|AAN66357.1|AE016264_1
                           (AE016776) glutamyl-tRNA reductase [Pseudomonas putida KT2440]
                           426
                           85.44
                           93.42
                           5
                           1801.0
     

     
     
81574
                           gb|AAO54637.1|
                           (AE016859) glutamyl-tRNA reductase [Pseudomonas syringae pv. tomato str. DC3000]
                           426
                           84.27
                           91.31
                           5
                           1767.0
     

     






      

          
GC ORFID: 44439How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA4666Glimmer Score: 

          
Start: 4770476Stop: 4769208

          
Length: 1269


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA4666 translation of PA4666Homolog Bit Score 805.0

          
Other Homologs:  psyr_15may02_Scaffold3_revised_gene4295 (ORF: BLASTX 688.0), t_PA4666 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGGCCTTCATTGCCCTCGGCATCAATCACAAGACCGCCTCGGTGGCTGTCCGCGAGCGCGTGGCTTTCACT 
CCGGAACAGATGGTCGAGGCGCTGCAGCAGCTCTGCCGCCTGACCACCAGCCGCGAGGCGGCCATCCTGTCG 
ACCTGCAACCGGAGCGAACTCTACCTGGAGATCGACCATCCGACCGCGGACGACGTGCTCGCCTGGCTGGCG 
GACTACCATCGTCTGACCCTCGACGAGTTGCGCGCCTGCGCCTATGTGCACCAGGACGAAGACGCGGTGCGC 
CACATGATGCGCGTGGCCTCCGGCCTCGACTCGATGGTCCTCGGCGAGCCGCAGATCCTCGGCCAGATGAAG 
TCGGCCTACGCCGTCGCGCGCGAGGCCGGCACCGTCGGTCCGCTGCTAGGCCGCCTGTTCCAGGCCACCTTC 
AGTACGGCGAAGACCGTCCGCACCGATACCGCCATCGGCGAGAACCCGGTGTCCGTGGCGTTCGCCGCGGTG 
AGCCTGGCCAAGCAGATCTTCAGCGACCTGCACCGCAGCCAGGCGCTGCTGATCGGCGCCGGCGAGACCATC 
ACCCTGGTCGCGCGCCACCTGTTCGAGCAAGGCGTGAAGCGCATCGTGGTGGCCAACCGCACCCTGGAGCGC 
GCCAGCCTGCTCGCCGAGCAGTTCGGCGCCCATGCGGTGCTGCTTTCGGAGATACCCGAGGAGTTGGCCAAC 
AGCGATATCGTCATCAGCTCCACTGCTAGCCAGTTGCCGATCCTCGGCAAGGGCGCCGTGGAGCGCGCGCTG 
AAGCAGCGCAAGCACAAGCCGATGTTCATGGTCGACATCGCCGTGCCGCGCGACATCGAGCCGGAAGTCGGC 
GAACTGGACGACGTCTACCTCTATAGCGTCGACGACCTCCACGAAGTAGTGGCGGAGAATCTCAAGAGCCGC 
CAGGGCGCTGCCCAGGCCGCCGAGGAACTGGTCGGCAGCGGCGTCGCCGAGTTCATGCAGCGCCTGCGCGAA 
CTGGCTGCGGTGGACGTGCTGCGCGCCTATCGGCAGCAGGCCGAGCGCCTGCGCGATGAAGAACTGGGCAAG 
GCCCAGCGGCAACTGGCCAACGGTGCCGACCCGGCCGAGGTGATGGCCCAGTTGGCCCGCGGCCTGACCAAC 
AAACTGCTGCACGCGCCCAGCGTGCAGATGAAGAAAATGTCCGCCGAGGGCCGCATCGACGCGCTGGCCCTG 
GCCCAGGAGCTGTTCGCCCTCGACGAGGGCGCGCCGCGCCACTGA