ViewGene
Locus Name:
GeneID:
Location:

GeneID:1422
TrivialName: PA14_41510
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_41510
PAO1 Ortholog Locus:PA1783
Sequence Length:1212
Protein Length:403
Start: 3702898
Stop: 3704109
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG05172.1|AE004604_2 (AE004604) nitrate transporter [Pseudomonas aeruginosa PAO1]
Identities = 403/403 (100%), Positives = 403/403 (100%)
Sequence:
ATGAACAACAGCTTCTGGAAGGCCGGCCATGCGCCGACCCTGTTCGCGGCGTTTCTCTATTTCGACCTGAGC
TTCATGGTCTGGTACGTGCTCGGCCCGCTGGGGGTGCAGATTGCCGGCGACCTGCAACTGAGCACCCAACAG
CGCGCGATGATGGTCGCCACGCCGATCCTCGCCGGCGCCGTACTGCGCTTCCTGCTCGGCCTGCTGGCCGAT
CGCTGGTCGCCGAAGAGCGCCGGCATCCTCGCCCAGGTCATCGTCATCGCCGCCCTGGCGGCCGCCTGGCTG
CTGGGCGTGAACAGCTATGCGCAGGCACTGCTGCTGGGGTTGTTCCTCGGCTTCGCCGGGGCATCCTTCGCC
GTGGCCCTGCCGCTCGCATCGCAATGGTACCCGCCGCAACACCAGGGCAAGGCCATGGGCATCGCCGGCGCG
GGCAACTCCGGCACCGTGCTCGCCGCCCTCTTCGCCCCCGGCCTGGCCGCCGCGTTCGGCTGGAACAACGTG
TTCGGCTTCGCCCTGCTGCCGCTCGTCCTGACCCTCCTGGTGTTCGCCATGGTCGCGCGCAACGCCCCGCAG
CGGCCGGCGCCCAAGGCCATGGGCGATTACCTGAAAGCCCTCGGCGACCGCGACAGCTGGTGGTTCATGTTC
TTCTACAGCGTCACCTTCGGCGGCTTCCTCGGCCTGGCCAGCACCCTGCCCGGCTATTTCCACGACCAGTAC
GGCCTGAACCCGGTGACCGCCGGCTACTACACCGCCGCCTGCGTCTTCGCCGGCAGCCTGATGCGCCCGCTC
GGTGGCGCCCTCGCCGATCGCATCGGCGGCATCCGCAGCCTGCTGATGGTGTACACCCTGGCCGCCATCTGC
ATCGCCGCGGTCGGTTTCCACTTGCCCAGCGCGATGGCCGCCCTCGGCCTGTTCGTGGTCGCCATGCTCAGT
CTCGGTGCCGGCAATGGCGCGGTGTTCCAGTTGGTACCGCAGCGCTTCCACAAGGAAATCGGCGTGATGACC
GGGCTGATCGGCATGGCCGGCGGCATCGGCGGCTTCTGCCTGACTGCCGGCCTCGGCGCGATCAAGCAAAGC
ACCGGCGACTACCAGCTCGGCCTGTGGCTGTTCGCCAGTCTCGGCGTGCTGGCCTGGTTCGGCCTCCACGGG
GTGAAGCGGCGCTGGCGCACCACCTGGGGCTCGGCCGCGGTCACCGCCGCGCGGGTCTGA
Translation:
MNNSFWKAGHAPTLFAAFLYFDLSFMVWYVLGPLGVQIAGDLQLSTQQRAMMVATPILAGAVLRFLLGLLAD
RWSPKSAGILAQVIVIAALAAAWLLGVNSYAQALLLGLFLGFAGASFAVALPLASQWYPPQHQGKAMGIAGA
GNSGTVLAALFAPGLAAAFGWNNVFGFALLPLVLTLLVFAMVARNAPQRPAPKAMGDYLKALGDRDSWWFMF
FYSVTFGGFLGLASTLPGYFHDQYGLNPVTAGYYTAACVFAGSLMRPLGGALADRIGGIRSLLMVYTLAAIC
IAAVGFHLPSAMAALGLFVVAMLSLGAGNGAVFQLVPQRFHKEIGVMTGLIGMAGGIGGFCLTAGLGAIKQS
TGDYQLGLWLFASLGVLAWFGLHGVKRRWRTTWGSAAVTAARV*
AnnotationID:1431GeneID:1422
AnnotatorUID: diggins
Modification Date/Time: 2005-05-11 08:08:32
Gene Name:nasA
Confidence Code:2
GeneProduct:nitrate transporter
Cell Localization:(8) Periplasmic
Synonyms:nasT
Cell Localization Confidence Code:2
MolecularFunction:
Functional Category:(8) Central intermediary metabolism
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:COG2223
Secondary Functional Category(ies):transport of small molecules
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAN67706.1|AE016401_9 (AE016781) nitrate transporter [Pseudomonas putida KT2440]
          Length = 411

 Score =  697 bits (1798), Expect = 0.0
 Identities = 341/403 (84%), Positives = 368/403 (91%)

>gb|AAO55818.1| (AE016864) nitrate transporter [Pseudomonas syringae pv. tomato
           str. DC3000]
          Length = 403

 Score =  675 bits (1742), Expect = 0.0
 Identities = 331/403 (82%), Positives = 361/403 (89%)

>gb|AAM41295.1| (AE012305) nitrate transporter [Xanthomonas campestris pv.
           campestris str. ATCC 33913]
          Length = 411

 Score =  533 bits (1374), Expect = e-151
 Identities = 270/404 (66%), Positives = 313/404 (77%), Gaps = 3/404 (0%)






 
Structural Features:
COG2223, NarK, Nitrate/nitrite transporter 
[Inorganic ion transport and metabolism]
CD-Length = 417 residues,  99.5% aligned
Score =  313 bits (804), Expect = 2e-86
Identities = 151/415 (36%), Positives = 216/415 (52%), Gaps = 13/415 (3%)






   
Genomic Context:






   
Comment:


















    ReferenceID:2847




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:19992




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:37134




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:1422_cdd.html


    		




 
URL:

















    ReferenceID:37812




  
Author/Investigator(s):
        Gutierrez JC, Ramos F, Ortner L, Tortolero M.





  
Title:
        nasST, two genes involved in the induction of the assimilatory nitrite-nitrate reductase operon (nasAB) of Azotobacter vinelandii.




  
PubMed:
        8748040


MedLine:
        




    
Source:
Mol Microbiol. 1995 Nov;18(3):579-91.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=8748040

















    ReferenceID:37816




  
Author/Investigator(s):
        Lin JT, Goldman BS, Stewart V.





  
Title:
        Structures of genes nasA and nasB, encoding assimilatory nitrate and nitrite reductases in Klebsiella pneumoniae M5al.




  
PubMed:
        8468296


MedLine:
        




    
Source:
J Bacteriol. 1993 Apr;175(8):2370-8.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=8468296

















    ReferenceID:37817




  
Author/Investigator(s):
        Ogawa K, Akagawa E, Yamane K, Sun ZW, LaCelle M, Zuber P, Nakano MM.





  
Title:
        The nasB operon and nasA gene are required for nitrate/nitrite assimilation in Bacillus subtilis.




  
PubMed:
        7868621


MedLine:
        




    
Source:
J Bacteriol. 1995 Mar;177(5):1409-13.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=7868621









           
Homologs By Global Alignment 

           

       



     
Gene ID:1422
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
2581
                           PA1783_tr
                           translation of PA1783
                           403
                           99.99
                           99.99
                           0
                           2086.0
     

     
     
81295
                           gb|AAG05172.1|AE004604_2
                           (AE004604) nitrate transporter [Pseudomonas aeruginosa PAO1]
                           403
                           99.99
                           99.99
                           0
                           2086.0
     

     
     
81296
                           gb|AAD22549.1|AF112870_1
                           (AF112870) nitrate transporter [Pseudomonas aeruginosa]
                           403
                           98.75
                           99.00
                           0
                           2060.0
     

     
     
81297
                           gb|AAN67706.1|AE016401_9
                           (AE016781) nitrate transporter [Pseudomonas putida KT2440]
                           411
                           82.96
                           89.53
                           8
                           1798.0
     

     
     
81298
                           gb|AAO55818.1|
                           (AE016864) nitrate transporter [Pseudomonas syringae pv. tomato str. DC3000]
                           403
                           82.13
                           89.57
                           0
                           1742.0
     

     






      

          
GC ORFID: 44424How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA1783Glimmer Score: 

          
Start: 1251011Stop: 1249800

          
Length: 1212


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA1783 translation of PA1783Homolog Bit Score 808.0

          
Other Homologs:  psyr_15may02_Scaffold1_revised_gene365 (ORF: BLASTX 674.0), t_PA1783 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGAACAACAGCTTCTGGAAGGCCGGCCATGCGCCGACCCTGTTCGCGGCGTTTCTCTATTTCGACCTGAGC 
TTCATGGTCTGGTACGTGCTCGGCCCGCTGGGGGTGCAGATTGCCGGCGACCTGCAACTGAGCACCCAACAG 
CGCGCGATGATGGTCGCCACGCCGATCCTCGCCGGCGCCGTACTGCGCTTCCTGCTCGGCCTGCTGGCCGAT 
CGCTGGTCGCCGAAGAGCGCCGGCATCCTCGCCCAGGTCATCGTCATCGCCGCCCTGGCGGCCGCCTGGCTG 
CTGGGCGTGAACAGCTATGCGCAGGCACTGCTGCTGGGGTTGTTCCTCGGCTTCGCCGGGGCATCCTTCGCC 
GTGGCCCTGCCGCTCGCATCGCAATGGTACCCGCCGCAACACCAGGGCAAGGCCATGGGCATCGCCGGCGCG 
GGCAACTCCGGCACCGTGCTCGCCGCCCTCTTCGCCCCCGGCCTGGCCGCCGCGTTCGGCTGGAACAACGTG 
TTCGGCTTCGCCCTGCTGCCGCTCGTCCTGACCCTCCTGGTGTTCGCCATGGTCGCGCGCAACGCCCCGCAG 
CGGCCGGCGCCCAAGGCCATGGGCGATTACCTGAAAGCCCTCGGCGACCGCGACAGCTGGTGGTTCATGTTC 
TTCTACAGCGTCACCTTCGGCGGCTTCCTCGGCCTGGCCAGCACCCTGCCCGGCTATTTCCACGACCAGTAC 
GGCCTGAACCCGGTGACCGCCGGCTACTACACCGCCGCCTGCGTCTTCGCCGGCAGCCTGATGCGCCCGCTC 
GGTGGCGCCCTCGCCGATCGCATCGGCGGCATCCGCAGCCTGCTGATGGTGTACACCCTGGCCGCCATCTGC 
ATCGCCGCGGTCGGTTTCCACTTGCCCAGCGCGATGGCCGCCCTCGGCCTGTTCGTGGTCGCCATGCTCAGT 
CTCGGTGCCGGCAATGGCGCGGTGTTCCAGTTGGTACCGCAGCGCTTCCACAAGGAAATCGGCGTGATGACC 
GGGCTGATCGGCATGGCCGGCGGCATCGGCGGCTTCTGCCTGACTGCCGGCCTCGGCGCGATCAAGCAAAGC 
ACCGGCGACTACCAGCTCGGCCTGTGGCTGTTCGCCAGTCTCGGCGTGCTGGCCTGGTTCGGCCTCCACGGG 
GTGAAGCGGCGCTGGCGCACCACCTGGGGCTCGGCCGCGGTCACCGCCGCGCGGGTCTGA