ViewGene
Locus Name:
GeneID:
Location:

GeneID:1418
TrivialName: PA14_64960
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_64960
PAO1 Ortholog Locus:PA4919
Sequence Length:1200
Protein Length:399
Start: 5789851
Stop: 5791050
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG08304.1|AE004905_2 (AE004905) nicotinate phosphoribosyltransferase [Pseudomonas aeruginosa PAO1]
Identities = 399/399 (100%), Positives = 399/399 (100%)
Sequence:
ATGGCCGAAAGCGTCTTTGCCGAGCGCATCGTGCAGAACCTGCTCGATACCGATTTCTACAAGCTGACCATG
ATGCAGGCGGTGCTGCACAACTACCCCAACGCCGAAGTGGAGTGGGAATTCCGCTGCCGCAACGCCGAGGAC
CTGCGTCCGTACCTGGCGGAGATCCGCTACCAGATCGAGCGCCTCGCCGAAGTCGAGGTAACCGCCGACCAG
CTCGCCTTCCTTGAACGCATCCCGTTCATGAAGCCGGACTTCATCCGCTTCCTCAGCCTGTTCCGCTTCAAC
CTGCGCTACGTGCACACCGGCATCGAGGATGGCCAACTGGCAATCCGCCTGCGCGGCCCATGGCTGCACGTG
ATCCTCTTCGAGGTGCCGCTGCTGGCCATCGTCAGCGAGGTGCGCAACCGTTATCGCTACCGCGAGGTGGTC
CTGGAACAGGTCGGCGAGCAGCTCTATCGCAAGCTCGACTGGCTCTCCGCCCAGGCCAGCAGCGAGGAACTC
GCGGAATTCCAGGTCGCCGACTTCGGCACCCGCCGGCGCTTCTCCTACCGCACCCAGGAGGAAGTGGTGCAT
ATCCTCAAGCGCGACTTCCCCGGGCGCTTCGTTGGCACCAGCAACGTGCACCTGGCCCGCGAGTACGATCTC
AAGCCGATCGGCACCATGGCCCACGAATGGCTGATGGCCCACCAGCAACTCGGCCCGCGGCTGGTCGACAGC
CAGCAGGCGGCGCTGGACTGCTGGGTCCGCGAGTACCGCGGGCAGCTCGGCATAGCCCTGACCGACTGCATT
ACCATGGACGCCTTCCTCGACGACTTCGACCTGTACTTCGCCAAGCTCTTCGACGGCCTGCGCCACGATTCG
GGCGACCCGCTGGCCTGGGCGGAGAAAGCCATCGCCCACTACCGCCGCCTGGGTATCGACCCGCTGAGCAAG
ACCCTGGTGTTCTCCGACGGGCTGGACATGCCCAAGGCGTTGCAGCTGTTCCGCGCGCTACGTGGCAAGATC
AACGTCAGCTTCGGCATCGGCACCAACCTGACCTGCGACATCCCGGGGGTCGAGCCGATGAACATCGTCCTG
AAGATGACGGCCTGCAACGGCCATCCGGTGGCGAAGATATCCGACGCCCCGGGCAAGACCCAATGCCGCGAC
GAGAACTTCGTCGCCTACCTCCGCCACGTCTTCAACGTGCCGGCCTAA
Translation:
MAESVFAERIVQNLLDTDFYKLTMMQAVLHNYPNAEVEWEFRCRNAEDLRPYLAEIRYQIERLAEVEVTADQ
LAFLERIPFMKPDFIRFLSLFRFNLRYVHTGIEDGQLAIRLRGPWLHVILFEVPLLAIVSEVRNRYRYREVV
LEQVGEQLYRKLDWLSAQASSEELAEFQVADFGTRRRFSYRTQEEVVHILKRDFPGRFVGTSNVHLAREYDL
KPIGTMAHEWLMAHQQLGPRLVDSQQAALDCWVREYRGQLGIALTDCITMDAFLDDFDLYFAKLFDGLRHDS
GDPLAWAEKAIAHYRRLGIDPLSKTLVFSDGLDMPKALQLFRALRGKINVSFGIGTNLTCDIPGVEPMNIVL
KMTACNGHPVAKISDAPGKTQCRDENFVAYLRHVFNVPA*
AnnotationID:1427GeneID:1418
AnnotatorUID: diggins
Modification Date/Time: 2005-04-26 07:07:52
Gene Name:pncB1
Confidence Code:2
GeneProduct:nicotinate phosphoribosyltransferase
Cell Localization:()
Synonyms:pncB
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(4) Biosynthesis of cofactors, prosthetic groups and carriers
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:cd01401,pfam04095,COG1488,cd01567
Secondary Functional Category(ies):coenzyme metabolism
EC Number:2.4.2.11
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAN70437.1|AE016685_2 (AE016792) nicotinate phosphoribosyltransferase [Pseudomonas putida
           KT2440]
          Length = 402

 Score =  650 bits (1676), Expect = 0.0
 Identities = 312/399 (78%), Positives = 352/399 (88%)

>gb|AAO58349.1| (AE016873) nicotinate phosphoribosyltransferase [Pseudomonas
           syringae pv. tomato str. DC3000]
          Length = 407

 Score =  643 bits (1658), Expect = 0.0
 Identities = 305/398 (76%), Positives = 349/398 (87%)

>gb|AAM86309.1|AE013878_6 (AE013878) nicotinate phosphoribosyltransferase [Yersinia pestis
           KIM]
          Length = 416

 Score =  413 bits (1061), Expect = e-114
 Identities = 207/392 (52%), Positives = 268/392 (68%), Gaps = 1/392 (0%)






 
Structural Features:
cd01401, PncB_like, Nicotinate phosphoribosyltransferase (NAPRTase), related to PncB.
CD-Length = 377 residues,  99.7% aligned
Score =  540 bits (1394), Expect = 7e-155

pfam04095, NAPRTase, Nicotinate phosphoribosyltransferase (NAPRTase) family.
CD-Length = 414 residues, 100.0% aligned
Score =  444 bits (1142), Expect = 1e-125
Identities = 177/423 (41%), Positives = 234/423 (55%), Gaps = 44/423 (10%)

COG1488, PncB, Nicotinic acid phosphoribosyltransferase [Coenzyme metabolism]
CD-Length = 405 residues,  93.1% aligned
Score =  361 bits (929), Expect = 6e-101
Identities = 155/407 (38%), Positives = 205/407 (50%), Gaps = 39/407 (9%)

cd01567, NAPRTase_PncB, Nicotinate phosphoribosyltransferase (NAPRTase) family. 
CD-Length = 343 residues, 100.0% aligned
Score =  287 bits (735), Expect = 2e-78






   
Genomic Context:






   
Comment:


















    ReferenceID:2839




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:19984




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:35138




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:1418_cdd.html


    		




 
URL:

















    ReferenceID:35927




  
Author/Investigator(s):
        Wubbolts MG, Terpstra P, van Beilen JB, Kingma J, Meesters HA, Witholt B.





  
Title:
        Variation of cofactor levels in Escherichia coli. Sequence analysis and expression of the pncB gene encoding nicotinic acid phosphoribosyltransferase.




  
PubMed:
        2211655


MedLine:
        




    
Source:
J Biol Chem. 1990 Oct 15;265(29):17665-72.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=2211655

















    ReferenceID:35928




  
Author/Investigator(s):
        Vinitsky A, Teng H, Grubmeyer CT.





  
Title:
        Cloning and nucleic acid sequence of the Salmonella typhimurium pncB gene and structure of nicotinate phosphoribosyltransferase.




  
PubMed:
        1987148


MedLine:
        




    
Source:
J Bacteriol. 1991 Jan;173(2):536-40.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=1987148









           
Homologs By Global Alignment 

           

       



     
Gene ID:1418
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
2575
                           PA4919_tr
                           translation of PA4919
                           399
                           99.99
                           99.99
                           0
                           2090.0
     

     
     
81215
                           gb|AAG08304.1|AE004905_2
                           (AE004905) nicotinate phosphoribosyltransferase [Pseudomonas aeruginosa PAO1]
                           399
                           99.99
                           99.99
                           0
                           2090.0
     

     
     
81216
                           gb|AAN70437.1|AE016685_2
                           (AE016792) nicotinate phosphoribosyltransferase [Pseudomonas putida KT2440]
                           402
                           77.61
                           87.56
                           3
                           1676.0
     

     
     
2576
                           PA4376_tr
                           translation of PA4376
                           399
                           77.19
                           86.71
                           1
                           1653.0
     

     
     
81218
                           gb|AAG07764.1|AE004853_7
                           (AE004853) nicotinate phosphoribosyltransferase [Pseudomonas aeruginosa PAO1]
                           399
                           77.19
                           86.71
                           1
                           1653.0
     

     






      

          
GC ORFID: 44420How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA4919Glimmer Score: 

          
Start: 5053532Stop: 5054731

          
Length: 1200


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TAATruncated Stop: 

          
Homolog: t_PA4919 translation of PA4919Homolog Bit Score 809.0

          
Other Homologs:  psyr_15may02_Scaffold8_revised_gene7783 (ORF: BLASTX 646.0), t_PA4376 (ORF: BLASTX 641.0), t_PA4919 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGGCCGAAAGCGTCTTTGCCGAGCGCATCGTGCAGAACCTGCTCGATACCGATTTCTACAAGCTGACCATG 
ATGCAGGCGGTGCTGCACAACTACCCCAACGCCGAAGTGGAGTGGGAATTCCGCTGCCGCAACGCCGAGGAC 
CTGCGTCCGTACCTGGCGGAGATCCGCTACCAGATCGAGCGCCTCGCCGAAGTCGAGGTAACCGCCGACCAG 
CTCGCCTTCCTTGAACGCATCCCGTTCATGAAGCCGGACTTCATCCGCTTCCTCAGCCTGTTCCGCTTCAAC 
CTGCGCTACGTGCACACCGGCATCGAGGATGGCCAACTGGCAATCCGCCTGCGCGGCCCATGGCTGCACGTG 
ATCCTCTTCGAGGTGCCGCTGCTGGCCATCGTCAGCGAGGTGCGCAACCGTTATCGCTACCGCGAGGTGGTC 
CTGGAACAGGTCGGCGAGCAGCTCTATCGCAAGCTCGACTGGCTCTCCGCCCAGGCCAGCAGCGAGGAACTC 
GCGGAATTCCAGGTCGCCGACTTCGGCACCCGCCGGCGCTTCTCCTACCGCACCCAGGAGGAAGTGGTGCAT 
ATCCTCAAGCGCGACTTCCCCGGGCGCTTCGTTGGCACCAGCAACGTGCACCTGGCCCGCGAGTACGATCTC 
AAGCCGATCGGCACCATGGCCCACGAATGGCTGATGGCCCACCAGCAACTCGGCCCGCGGCTGGTCGACAGC 
CAGCAGGCGGCGCTGGACTGCTGGGTCCGCGAGTACCGCGGGCAGCTCGGCATAGCCCTGACCGACTGCATT 
ACCATGGACGCCTTCCTCGACGACTTCGACCTGTACTTCGCCAAGCTCTTCGACGGCCTGCGCCACGATTCG 
GGCGACCCGCTGGCCTGGGCGGAGAAAGCCATCGCCCACTACCGCCGCCTGGGTATCGACCCGCTGAGCAAG 
ACCCTGGTGTTCTCCGACGGGCTGGACATGCCCAAGGCGTTGCAGCTGTTCCGCGCGCTACGTGGCAAGATC 
AACGTCAGCTTCGGCATCGGCACCAACCTGACCTGCGACATCCCGGGGGTCGAGCCGATGAACATCGTCCTG 
AAGATGACGGCCTGCAACGGCCATCCGGTGGCGAAGATATCCGACGCCCCGGGCAAGACCCAATGCCGCGAC 
GAGAACTTCGTCGCCTACCTCCGCCACGTCTTCAACGTGCCGGCCTAA