ViewGene
Locus Name:
GeneID:
Location:

GeneID:1417
TrivialName: PA14_50540
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_50540
PAO1 Ortholog Locus:PA1072
Sequence Length:1254
Protein Length:417
Start: 4489252
Stop: 4490505
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG04461.1|AE004539_3 (AE004539) branched-chain amino acid transport protein BraE [Pseudomonas aeruginosa PAO1]
Identities = 416/417 (99%), Positives = 416/417 (99%)
Sequence:
ATGAGCCAGTCCCTCAAGCGCGCGCTGTTCAGCGCCCTCCTCGTGATCCTGGTGTCCTATCCGATCCTCGGC
CTGAAGCTGCGCACCGTCGGCATCAAGCTCGAGGTCCTCGGCGCCGATGCGCAGACCCTCTGGACCATCGCC
GTGGCCGCCCTGGCCATGTTCGTCTGGCAGCTGTTCCGCGACCGCATCCCGCTCAAGCTGGGTCGCGGCGTC
GGCTACAAGGTCAACGGCAGCGGCCTGAAGAACTTCCTCAGCCTGCCGTCGACCCAGCGCTGGGCGGTACTC
GCCCTGGTCGTGGTGGCTTTCGTCTGGCCGTTCTTCGCCTCGCGCGGGGCGGTGGACATCGCCACCCTGATC
CTGATCTACGTGATGCTCGGCATCGGCCTGAACATCGTGGTCGGCTTGGCCGGCCTGCTCGACCTCGGCTAC
GTCGGCTTCTACGCGGTGGGTGCCTACACCTACGCGCTGCTCGCCGAGTACGCCGGGTTCGGCTTCTGGACC
GCCCTGCCGATCGCCGGGATGATGGCCGCGCTGTTCGGTTTCCTCCTCGGTTTCCCGGTGCTGCGCCTGCGC
GGCGACTACCTGGCGATCGTGACCCTCGGCTTCGGCGAGATCATCCGCATCCTGCTGCGCAACATGACCGAG
ATCACCGGCGGCCCCAACGGCATCGGCTCGATCCCCAAGCCGACCCTGTTCGGCCTGACCTTCGAACGCCGC
GCGCCGGAAGGCATGCAGACCTTCCACGAGTTCTTCGGCATCGCCTACAACACCAACTACAAGGTCATCCTG
CTCTACGTGGTGGCCCTGCTGCTGGTGCTGCTGGCCCTGTTCGTGATCAACCGGCTGATGCGCATGCCGATC
GGTCGCGCCTGGGAAGCGCTGCGCGAGGACGAAGTGGCCTGCCGCGCTCTCGGTCTCAACCCGACCATCGTC
AAGCTCTCCGCCTTCACCATCGGCGCCAGCTTCGCCGGTTTCGCCGGCAGCTTCTTCGCCGCCCGCCAGGGC
CTGGTGACGCCTGAGTCCTTCACCTTCATCGAGTCGGCGATGATCCTTGCGATCGTCGTGCTCGGCGGCATG
GGTTCGCAGCTCGGCGTGATACTCGCCGCGGTGGTGATGGTGCTGCTCCAGGAAATGCGCGGGTTCAACGAA
TACCGCATGCTGATCTTCGGCCTGACCATGATCGTGATGATGATCTGGCGTCCCCAGGGACTGCTGCCGATG
CAACGCCCGCACCTGGAGCTGAAGCCATGA
Translation:
MSQSLKRALFSALLVILVSYPILGLKLRTVGIKLEVLGADAQTLWTIAVAALAMFVWQLFRDRIPLKLGRGV
GYKVNGSGLKNFLSLPSTQRWAVLALVVVAFVWPFFASRGAVDIATLILIYVMLGIGLNIVVGLAGLLDLGY
VGFYAVGAYTYALLAEYAGFGFWTALPIAGMMAALFGFLLGFPVLRLRGDYLAIVTLGFGEIIRILLRNMTE
ITGGPNGIGSIPKPTLFGLTFERRAPEGMQTFHEFFGIAYNTNYKVILLYVVALLLVLLALFVINRLMRMPI
GRAWEALREDEVACRALGLNPTIVKLSAFTIGASFAGFAGSFFAARQGLVTPESFTFIESAMILAIVVLGGM
GSQLGVILAAVVMVLLQEMRGFNEYRMLIFGLTMIVMMIWRPQGLLPMQRPHLELKP*
AnnotationID:1426GeneID:1417
AnnotatorUID: danlee
Modification Date/Time: 2004-05-19 17:05:12
Gene Name:braE
Confidence Code:1
GeneProduct:branched-chain amino acid transport protein BraE
Cell Localization:(6) Outer membrane protein
Synonyms:PA1072
Cell Localization Confidence Code:1
MolecularFunction:
Functional Category:(27) Transport of small molecules
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:COG4177
Secondary Functional Category(ies):Membrane proteins
EC Number:
Status:ACTIVE
Pathway:






 
Homology:






 
Structural Features:
COG4177, LivM, ABC-type branched-chain amino acid transport system, permease 
component [Amino acid transport and metabolism]
     CD-Length = 314 residues,  96.2% aligned
     Score =  212 bits (540), Expect = 9e-56






   
Genomic Context:
Adjacent to genes BraC, BraD, BraF, and BraG, reported to constitute a
transport system for branched-chain amino acids 






   
Comment:


















    ReferenceID:2837




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:19982




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:32218




  
Author/Investigator(s):
        Hoshino T, Kose K.





  
Title:
        Cloning, nucleotide sequences, and identification of products of the Pseudomonas aeruginosa PAO bra genes, which encode the high-affinity branched-chain amino acid transport system.




  
PubMed:
        91008919


MedLine:
        




    
Source:
J Bacteriol. 1990 Oct;172(10):5531-9






    
Reference Type:Journal 







    
Data:


    		




 
URL:









           
Homologs By Global Alignment 

           

       



     
Gene ID:1417
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
2573
                           PA1072_tr
                           translation of PA1072
                           417
                           99.76
                           99.76
                           0
                           2089.0
     

     
     
81170
                           gb|AAG04461.1|AE004539_3
                           (AE004539) branched-chain amino acid transport protein BraE [Pseudomonas aeruginosa PAO1]
                           417
                           99.76
                           99.76
                           0
                           2089.0
     

     
     
81171
                           dbj|BAA14256.1|
                           (D90223) integral membrane protein [Pseudomonas aeruginosa]
                           417
                           99.76
                           99.76
                           0
                           2089.0
     

     
     
81172
                           gb|AAR19310.1|
                           (AY426537) putative branched-chain amino acid transporter permease protein [symbiont bacterium of Paederus fuscipes]
                           417
                           89.44
                           94.72
                           0
                           1897.0
     

     
     
81174
                           gb|AAN66764.1|AE016304_9
                           (AE016778) high-affinity branched-chain amino acid transport protein [Pseudomonas putida KT2440]
                           418
                           72.96
                           84.92
                           1
                           1551.0
     

     






      

          
GC ORFID: 44419How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA1072Glimmer Score: 

          
Start: 464656Stop: 463403

          
Length: 1254


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA1072 translation of PA1072Homolog Bit Score 809.0

          
Other Homologs:  psyr_15may02_Scaffold5_revised_gene6075 (ORF: BLASTX 602.0), psyr_15may02_Scaffold8_revised_gene7644 (ORF: BLASTX 410.0), t_PA4911 (ORF: BLASTX 408.0), t_PA1072 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGAGCCAGTCCCTCAAGCGCGCGCTGTTCAGCGCCCTCCTCGTGATCCTGGTGTCCTATCCGATCCTCGGC 
CTGAAGCTGCGCACCGTCGGCATCAAGCTCGAGGTCCTCGGCGCCGATGCGCAGACCCTCTGGACCATCGCC 
GTGGCCGCCCTGGCCATGTTCGTCTGGCAGCTGTTCCGCGACCGCATCCCGCTCAAGCTGGGTCGCGGCGTC 
GGCTACAAGGTCAACGGCAGCGGCCTGAAGAACTTCCTCAGCCTGCCGTCGACCCAGCGCTGGGCGGTACTC 
GCCCTGGTCGTGGTGGCTTTCGTCTGGCCGTTCTTCGCCTCGCGCGGGGCGGTGGACATCGCCACCCTGATC 
CTGATCTACGTGATGCTCGGCATCGGCCTGAACATCGTGGTCGGCTTGGCCGGCCTGCTCGACCTCGGCTAC 
GTCGGCTTCTACGCGGTGGGTGCCTACACCTACGCGCTGCTCGCCGAGTACGCCGGGTTCGGCTTCTGGACC 
GCCCTGCCGATCGCCGGGATGATGGCCGCGCTGTTCGGTTTCCTCCTCGGTTTCCCGGTGCTGCGCCTGCGC 
GGCGACTACCTGGCGATCGTGACCCTCGGCTTCGGCGAGATCATCCGCATCCTGCTGCGCAACATGACCGAG 
ATCACCGGCGGCCCCAACGGCATCGGCTCGATCCCCAAGCCGACCCTGTTCGGCCTGACCTTCGAACGCCGC 
GCGCCGGAAGGCATGCAGACCTTCCACGAGTTCTTCGGCATCGCCTACAACACCAACTACAAGGTCATCCTG 
CTCTACGTGGTGGCCCTGCTGCTGGTGCTGCTGGCCCTGTTCGTGATCAACCGGCTGATGCGCATGCCGATC 
GGTCGCGCCTGGGAAGCGCTGCGCGAGGACGAAGTGGCCTGCCGCGCTCTCGGTCTCAACCCGACCATCGTC 
AAGCTCTCCGCCTTCACCATCGGCGCCAGCTTCGCCGGTTTCGCCGGCAGCTTCTTCGCCGCCCGCCAGGGC 
CTGGTGACGCCTGAGTCCTTCACCTTCATCGAGTCGGCGATGATCCTTGCGATCGTCGTGCTCGGCGGCATG 
GGTTCGCAGCTCGGCGTGATACTCGCCGCGGTGGTGATGGTGCTGCTCCAGGAAATGCGCGGGTTCAACGAA 
TACCGCATGCTGATCTTCGGCCTGACCATGATCGTGATGATGATCTGGCGTCCCCAGGGACTGCTGCCGATG 
CAACGCCCGCACCTGGAGCTGAAGCCATGA