ViewGene
Locus Name:
GeneID:
Location:

GeneID:1416
TrivialName: PA14_71560
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_71560
PAO1 Ortholog Locus:PA5421
Sequence Length:1200
Protein Length:399
Start: 6374808
Stop: 6376007
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG08806.1|AE004954_8 (AE004954) glutathione-independent formaldehyde dehydrogenase [Pseudomonas aeruginosa PAO1]
Identities = 399/399 (100%), Positives = 399/399 (100%)
Sequence:
ATGTCTGGCAATCGTGGTGTGGTCTATCTCGGCCCGGGCAAGGTCGAGGTGCAGAACATTCCCTATCCGAAG
ATGCAGGACCCGCAGGGCCGGCAGATCGACCACGGGGTGATCCTGCGGGTGGTCTCCACCAACATCTGCGGC
TCCGACCAGCACATGGTGCGCGGCCGCACCACCGCGCCGGAAGGCCTGGTGCTGGGCCACGAGATCACCGGC
GAGGTAGTGGAGATCGGGCGTGGCGTGGAAACCATGAAGATCGGCGACCTGGTCTCGGTACCGTTCAACGTC
GCTTGCGGCCATTGCCGTACCTGCAAGGAACAGCACACCGGCGTCTGCCTGACGGTCAATCCGGCGCGGGCC
GGCGGCGCCTACGGCTACGTCGACATGGGTGGCTGGGTCGGCGGCCAGGCCGAATACGTGCTGGTGCCCTAC
GCCGACTTCAACCTGCTGAAACTGCCCAACCGCGAAGCGGCGATGGAGAAGATCCGCGACCTGACCTGCCTT
TCGGACATCCTCCCCACCGGCTACCACGGCGCGGTCACCGCCGGCGTCGGCCCGGGCAGCACGGTCTACATC
GCCGGCGCCGGTCCGGTCGGCCTGGCCGCGGCGGCCTCGGCACGCCTGCTCGGCGCGGCGGTGGTGATCGTC
GGCGACGTCAACCCGACCCGCCTGGCCCATGCCAAGAAACAGGGCTTCGAGATCGCCGACCTGTCCAAGGAC
ACCCCGCTGCACGAACAGATCGCCGCTCTGTTGGGCGAGCCGGAGGTCGACTGCGCGGTCGACGCGGTCGGT
TTCGAGGCGCGCGGCCACGGCCATTCGGGCTCGCAGCAGGAAGCCCCGGCCACCGTGCTCAACTCGCTGATG
GGCATCACCCGGGTCGCCGGCAAGATCGGCATCCCCGGCCTGTACGTCACCGAAGACCCGGGCGCGGTGGAC
GCGGCCGCCAAGCACGGCGCCCTGAGCATCCGCTTCGGCCTGGGCTGGGCCAAGTCGCACAGCTTCCATACC
GGCCAGACCCCGGTGATGAAGTACAACCGCCAGCTGATGCAGGCGATCATGTGGGACCGGATCAAGATCGCC
GACATCGTCGGGGTGGAAGTCATCACCCTCGACGATGCGCCGAAAGGCTACGGCGAGTTCGACGCCGGGGTG
CCGAAGAAATTCGTCATCGACCCGCACAACCTGTTCCGCGCGGCCTGA
Translation:
MSGNRGVVYLGPGKVEVQNIPYPKMQDPQGRQIDHGVILRVVSTNICGSDQHMVRGRTTAPEGLVLGHEITG
EVVEIGRGVETMKIGDLVSVPFNVACGHCRTCKEQHTGVCLTVNPARAGGAYGYVDMGGWVGGQAEYVLVPY
ADFNLLKLPNREAAMEKIRDLTCLSDILPTGYHGAVTAGVGPGSTVYIAGAGPVGLAAAASARLLGAAVVIV
GDVNPTRLAHAKKQGFEIADLSKDTPLHEQIAALLGEPEVDCAVDAVGFEARGHGHSGSQQEAPATVLNSLM
GITRVAGKIGIPGLYVTEDPGAVDAAAKHGALSIRFGLGWAKSHSFHTGQTPVMKYNRQLMQAIMWDRIKIA
DIVGVEVITLDDAPKGYGEFDAGVPKKFVIDPHNLFRAA*
AnnotationID:1425GeneID:1416
AnnotatorUID: diggins
Modification Date/Time: 2005-03-17 14:02:30
Gene Name:fdhA
Confidence Code:2
GeneProduct:glutathione-independent formaldehyde dehydrogenase
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(2) Amino acid biosynthesis and metabolism
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:COG1063,pfam00107,COG1064
Secondary Functional Category(ies):
EC Number:1.2.1.46
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAO53999.1| (AE016857) glutathione-independent formaldehyde dehydrogenase
           [Pseudomonas syringae pv. tomato str. DC3000]
          Length = 399

 Score =  734 bits (1896), Expect = 0.0
 Identities = 356/396 (89%), Positives = 380/396 (95%)

>gb|AAN65959.1|AE016224_3 (AE016775) formaldehyde dehydrogenase, glutathione-independent
           [Pseudomonas putida KT2440]
          Length = 399

 Score =  713 bits (1840), Expect = 0.0
 Identities = 346/399 (86%), Positives = 368/399 (92%)

>emb|CAD17204.1| (AL646076) PROBABLE GLUTATHIONE-INDEPENDENT FORMALDEHYDE
           DEHYDROGENASE OXIDOREDUCTASE PROTEIN [Ralstonia
           solanacearum]
          Length = 398

 Score =  664 bits (1714), Expect = 0.0
 Identities = 328/399 (82%), Positives = 349/399 (87%), Gaps = 1/399 (0%)






 
Structural Features:
COG1063, Tdh, Threonine dehydrogenase and related Zn-dependent dehydrogenases 
[Amino acid transport and metabolism]
CD-Length = 350 residues,  99.7% aligned
Score =  207 bits (528), Expect = 2e-54
Identities = 132/397 (33%), Positives = 183/397 (46%), Gaps = 56/397 (14%)

pfam00107, ADH_zinc_N, Zinc-binding dehydrogenase. 
CD-Length = 341 residues,  99.1% aligned
Score =  172 bits (436), Expect = 7e-44
Identities = 105/388 (27%), Positives = 160/388 (41%), Gaps = 56/388 (14%)

COG1064, AdhP, Zn-dependent alcohol dehydrogenases
CD-Length = 339 residues,  98.2% aligned
Score =  121 bits (305), Expect = 1e-28
Identities = 99/394 (25%), Positives = 153/394 (38%), Gaps = 67/394 (17%)






   
Genomic Context:






   
Comment:


















    ReferenceID:2835




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:19980




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:34687




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:1416_cdd.html


    		




 
URL:

















    ReferenceID:35639




  
Author/Investigator(s):
        Bokranz M, Gutmann M, Kortner C, Kojro E, Fahrenholz F, Lauterbach F, Kroger A.





  
Title:
        Cloning and nucleotide sequence of the structural genes encoding the formate dehydrogenase of Wolinella succinogenes.




  
PubMed:
        1781728


MedLine:
        




    
Source:
Arch Microbiol. 1991;156(2):119-28.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=1781728

















    ReferenceID:35640




  
Author/Investigator(s):
        Schmitz RP, Diekert G.





  
Title:
        Purification and properties of the formate dehydrogenase and characterization of the fdhA gene of Sulfurospirillum multivorans.




  
PubMed:
        14610638


MedLine:
        




    
Source:
Arch Microbiol. 2003 Dec;180(6):394-401. Epub 2003 Nov 11.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=14610638









           
Homologs By Global Alignment 

           

       



     
Gene ID:1416
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
2572
                           PA5421_tr
                           translation of PA5421
                           399
                           99.99
                           99.99
                           0
                           2091.0
     

     
     
81155
                           gb|AAG08806.1|AE004954_8
                           (AE004954) glutathione-independent formaldehyde dehydrogenase [Pseudomonas aeruginosa PAO1]
                           399
                           99.99
                           99.99
                           0
                           2091.0
     

     
     
81156
                           gb|AAO53999.1|
                           (AE016857) glutathione-independent formaldehyde dehydrogenase [Pseudomonas syringae pv. tomato str. DC3000]
                           399
                           89.22
                           95.23
                           0
                           1894.0
     

     
     
81157
                           dbj|BAA04743.1|
                           (D21201) formaldehyde dehydrogenase [Pseudomonas putida]
                           399
                           87.96
                           93.73
                           0
                           1861.0
     

     
     
81158
                           gb|AAN65959.1|AE016224_3
                           (AE016775) formaldehyde dehydrogenase, glutathione-independent [Pseudomonas putida KT2440]
                           399
                           86.71
                           92.23
                           0
                           1840.0
     

     






      

          
GC ORFID: 44418How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA5421Glimmer Score: 

          
Start: 5638497Stop: 5639696

          
Length: 1200


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA5421 translation of PA5421Homolog Bit Score 810.0

          
Other Homologs:  psyr_15may02_Scaffold1_revised_gene1344 (ORF: BLASTX 295.0), t_PA5421 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGTCTGGCAATCGTGGTGTGGTCTATCTCGGCCCGGGCAAGGTCGAGGTGCAGAACATTCCCTATCCGAAG 
ATGCAGGACCCGCAGGGCCGGCAGATCGACCACGGGGTGATCCTGCGGGTGGTCTCCACCAACATCTGCGGC 
TCCGACCAGCACATGGTGCGCGGCCGCACCACCGCGCCGGAAGGCCTGGTGCTGGGCCACGAGATCACCGGC 
GAGGTAGTGGAGATCGGGCGTGGCGTGGAAACCATGAAGATCGGCGACCTGGTCTCGGTACCGTTCAACGTC 
GCTTGCGGCCATTGCCGTACCTGCAAGGAACAGCACACCGGCGTCTGCCTGACGGTCAATCCGGCGCGGGCC 
GGCGGCGCCTACGGCTACGTCGACATGGGTGGCTGGGTCGGCGGCCAGGCCGAATACGTGCTGGTGCCCTAC 
GCCGACTTCAACCTGCTGAAACTGCCCAACCGCGAAGCGGCGATGGAGAAGATCCGCGACCTGACCTGCCTT 
TCGGACATCCTCCCCACCGGCTACCACGGCGCGGTCACCGCCGGCGTCGGCCCGGGCAGCACGGTCTACATC 
GCCGGCGCCGGTCCGGTCGGCCTGGCCGCGGCGGCCTCGGCACGCCTGCTCGGCGCGGCGGTGGTGATCGTC 
GGCGACGTCAACCCGACCCGCCTGGCCCATGCCAAGAAACAGGGCTTCGAGATCGCCGACCTGTCCAAGGAC 
ACCCCGCTGCACGAACAGATCGCCGCTCTGTTGGGCGAGCCGGAGGTCGACTGCGCGGTCGACGCGGTCGGT 
TTCGAGGCGCGCGGCCACGGCCATTCGGGCTCGCAGCAGGAAGCCCCGGCCACCGTGCTCAACTCGCTGATG 
GGCATCACCCGGGTCGCCGGCAAGATCGGCATCCCCGGCCTGTACGTCACCGAAGACCCGGGCGCGGTGGAC 
GCGGCCGCCAAGCACGGCGCCCTGAGCATCCGCTTCGGCCTGGGCTGGGCCAAGTCGCACAGCTTCCATACC 
GGCCAGACCCCGGTGATGAAGTACAACCGCCAGCTGATGCAGGCGATCATGTGGGACCGGATCAAGATCGCC 
GACATCGTCGGGGTGGAAGTCATCACCCTCGACGATGCGCCGAAAGGCTACGGCGAGTTCGACGCCGGGGTG 
CCGAAGAAATTCGTCATCGACCCGCACAACCTGTTCCGCGCGGCCTGA