ViewGene
Locus Name:
GeneID:
Location:

GeneID:1411
TrivialName: PA14_56900
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_56900
PAO1 Ortholog Locus:PA4376
Sequence Length:1197
Protein Length:398
Start: 5071393
Stop: 5072589
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG07764.1|AE004853_7 (AE004853) nicotinate phosphoribosyltransferase [Pseudomonas aeruginosa PAO1]
Identities = 398/398 (100%), Positives = 398/398 (100%)
Sequence:
ATGGCcGAGAGCGCTTTTTCCGAACGTATCGTGCAGAACCTGCTGGATACCGACTTCTACAAGCTGACCATG
ATGCAGGCGGTGCTGCACAACTATCCCAACGCCGAGGTGGAGTGGGAGTTCCGCTGCCGCAACCAGGAAGAC
CTGCGCCTGTACCTGCCGGCCATCCGCGAACAACTGGAATACCTGGCGGGCCTGGCGATCAGCGACGAGCAA
CTGGCCTTCCTCGAACGGATTCCCTTCCTCGCGCCGGACTTCATCCGCTTCCTCGGGTTGTTCCGCTTCAAC
CCGCGCTACGTGCAGACCGGCATCGAGAACGACGAATTCTTCCTGCGCCTGAAAGGCCCCTGGCTGCACGTG
ATCCTCTTCGAAGTGCCGCTGCTGGCGATGATCAGCGAGGTGCGCAACCGCGCCCGCTACCCGGCCGCCACC
GTCGAGCAGGCGCGCGAACGCCTGCAGGAAAAGTTCGATTGGCTGCGCCGGGAGGCCAGCGCCGAGGAACTG
GCCGGCTTCAAGATGGCCGACTTCGGTACCCGCCGCCGCTTCTCCTATCGGGTCCACGAGGCGGTGGTGAGC
GGTCTGAAGGAGGACTTTCCGGGCTGCTTCGTCGGCACCAGCAACGTCCACCTGGCACGCAAGCTCGACCTC
AAGCCCCTCGGCACCATGGCCCACGAATGGCTGATGGCCCACCAGCAGCTCGGTCCGCGCCTGATCGACAGC
CAGAGTGCCGCGTTGGACTGCTGGGTCCGCGAATACCGTGGGCTGCTCGGCATCGCGCTGACCGACTGCATC
ACCACCGATGCCTTCCTGCGCGACTTCGACCTCTATTTCGCCAAGCTCTTCGACGGCCTCCGCCACGACTCG
GGCGACCCGTTGCTGTGGGCGGAAAAGACCATCGCCCACTACCTCAAGCTGGGCATCGACCCGCTGACCAAG
ACCCTGGTGTTCTCCGACGGCCTCGACCTGCCGCGGGCCCTGAAGATCTATCGCGCGTTGCAGGGCCGGATC
AACGTCAGCTTCGGCATAGGTACGCATTTCACCTGCGACCTGCCGGGCGTCGAGCCGATGAACATCGTGGTG
AAGATGAGCGCCTGCAACGGCCATCCGGTGGCGAAGATCTCCGATACGCCCGGCAAGGCGCAATGCCGGGAC
CCTGACTTCATCCACTACCTCAAGCACGTGTTCCAGGTCGCCTAG
Translation:
MAESAFSERIVQNLLDTDFYKLTMMQAVLHNYPNAEVEWEFRCRNQEDLRLYLPAIREQLEYLAGLAISDEQ
LAFLERIPFLAPDFIRFLGLFRFNPRYVQTGIENDEFFLRLKGPWLHVILFEVPLLAMISEVRNRARYPAAT
VEQARERLQEKFDWLRREASAEELAGFKMADFGTRRRFSYRVHEAVVSGLKEDFPGCFVGTSNVHLARKLDL
KPLGTMAHEWLMAHQQLGPRLIDSQSAALDCWVREYRGLLGIALTDCITTDAFLRDFDLYFAKLFDGLRHDS
GDPLLWAEKTIAHYLKLGIDPLTKTLVFSDGLDLPRALKIYRALQGRINVSFGIGTHFTCDLPGVEPMNIVV
KMSACNGHPVAKISDTPGKAQCRDPDFIHYLKHVFQVA*
AnnotationID:1420GeneID:1411
AnnotatorUID: diggins
Modification Date/Time: 2005-05-02 07:07:36
Gene Name:pncB2
Confidence Code:2
GeneProduct:nicotinate phosphoribosyltransferase
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(4) Biosynthesis of cofactors, prosthetic groups and carriers
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:pfam04095,COG1488,KOG2511
Secondary Functional Category(ies):
EC Number:2.4.2.11
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAO58349.1| (AE016873) nicotinate phosphoribosyltransferase [Pseudomonas
           syringae pv. tomato str. DC3000]
          Length = 407

 Score =  622 bits (1605), Expect = e-178
 Identities = 300/397 (75%), Positives = 343/397 (86%)


>gb|AAN70437.1|AE016685_2 (AE016792) nicotinate phosphoribosyltransferase [Pseudomonas putida
           KT2440]
          Length = 402

 Score =  616 bits (1589), Expect = e-176
 Identities = 302/397 (76%), Positives = 339/397 (85%)








 
Structural Features:
pfam04095, NAPRTase, Nicotinate phosphoribosyltransferase 
(NAPRTase) family.
CD-Length = 414 residues,  99.5% aligned
Score =  443 bits (1140), Expect = 5e-125
Identities = 184/421 (43%), Positives = 237/421 (56%), Gaps = 44/421 (10%)

COG1488, PncB, Nicotinic acid phosphoribosyltransferase 
[Coenzyme metabolism]
CD-Length = 405 residues,  92.8% aligned
Score =  364 bits (935), Expect = 3e-101
Identities = 156/406 (38%), Positives = 204/406 (50%), Gaps = 39/406 (9%)

KOG2511, Nicotinic acid phosphoribosyltransferase 
[Coenzyme transport and metabolism]
CD-Length = 420 residues,  90.7% aligned
Score =  178 bits (452), Expect = 2e-45
Identities = 115/411 (27%), Positives = 184/411 (44%), Gaps = 68/411 (16%)






   
Genomic Context:






   
Comment:


















    ReferenceID:2825




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:17134




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:1411_cdd.html


    		




 
URL:

















    ReferenceID:19970




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:32687




  
Author/Investigator(s):
        Wubbolts MG, Terpstra P, van Beilen JB, Kingma J, Meesters HA, Witholt B.





  
Title:
        Variation of cofactor levels in Escherichia coli. Sequence analysis and expression of the pncB gene encoding nicotinic acid phosphoribosyltransferase.




  
PubMed:
        2211655


MedLine:
        




    
Source:
J Biol Chem. 1990 Oct 15;265(29):17665-72.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=2211655

















    ReferenceID:32688




  
Author/Investigator(s):
        Baecker PA, Yung SG, Rodriguez M, Austin E, Andreoli AJ.





  
Title:
        Periplasmic localization of nicotinate phosphoribosyltransferase in Escherichia coli.




  
PubMed:
        346557


MedLine:
        




    
Source:
J Bacteriol. 1978 Mar;133(3):1108-12.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=346557

















    ReferenceID:32689




  
Author/Investigator(s):
        Kosaka A, Spivey HO, Gholson RK.





  
Title:
        Nicotinate phosphoribosyltransferase of yeast. Purification and properties.




  
PubMed:
        4324895


MedLine:
        




    
Source:
J Biol Chem. 1971 May 25;246(10):3277-83.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=4324895









           
Homologs By Global Alignment 

           

       



     
Gene ID:1411
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
2565
                           PA4376_tr
                           translation of PA4376
                           398
                           99.99
                           99.99
                           0
                           2096.0
     

     
     
81076
                           gb|AAG07764.1|AE004853_7
                           (AE004853) nicotinate phosphoribosyltransferase [Pseudomonas aeruginosa PAO1]
                           398
                           99.99
                           99.99
                           0
                           2096.0
     

     
     
2566
                           PA4919_tr
                           translation of PA4919
                           399
                           77.19
                           86.71
                           1
                           1653.0
     

     
     
81077
                           gb|AAG08304.1|AE004905_2
                           (AE004905) nicotinate phosphoribosyltransferase [Pseudomonas aeruginosa PAO1]
                           399
                           77.19
                           86.71
                           1
                           1653.0
     

     
     
81079
                           gb|AAN70437.1|AE016685_2
                           (AE016792) nicotinate phosphoribosyltransferase [Pseudomonas putida KT2440]
                           402
                           75.12
                           84.32
                           4
                           1588.0
     

     






      

          
GC ORFID: 44413How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA4376Glimmer Score: 

          
Start: 4335069Stop: 4336265

          
Length: 1197


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TAGTruncated Stop: 

          
Homolog: t_PA4376 translation of PA4376Homolog Bit Score 811.0

          
Other Homologs:  t_PA4919 (ORF: BLASTX 641.0), psyr_15may02_Scaffold8_revised_gene7783 (ORF: BLASTX 630.0), t_PA4376 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGGCcGAGAGCGCTTTTTCCGAACGTATCGTGCAGAACCTGCTGGATACCGACTTCTACAAGCTGACCATG 
ATGCAGGCGGTGCTGCACAACTATCCCAACGCCGAGGTGGAGTGGGAGTTCCGCTGCCGCAACCAGGAAGAC 
CTGCGCCTGTACCTGCCGGCCATCCGCGAACAACTGGAATACCTGGCGGGCCTGGCGATCAGCGACGAGCAA 
CTGGCCTTCCTCGAACGGATTCCCTTCCTCGCGCCGGACTTCATCCGCTTCCTCGGGTTGTTCCGCTTCAAC 
CCGCGCTACGTGCAGACCGGCATCGAGAACGACGAATTCTTCCTGCGCCTGAAAGGCCCCTGGCTGCACGTG 
ATCCTCTTCGAAGTGCCGCTGCTGGCGATGATCAGCGAGGTGCGCAACCGCGCCCGCTACCCGGCCGCCACC 
GTCGAGCAGGCGCGCGAACGCCTGCAGGAAAAGTTCGATTGGCTGCGCCGGGAGGCCAGCGCCGAGGAACTG 
GCCGGCTTCAAGATGGCCGACTTCGGTACCCGCCGCCGCTTCTCCTATCGGGTCCACGAGGCGGTGGTGAGC 
GGTCTGAAGGAGGACTTTCCGGGCTGCTTCGTCGGCACCAGCAACGTCCACCTGGCACGCAAGCTCGACCTC 
AAGCCCCTCGGCACCATGGCCCACGAATGGCTGATGGCCCACCAGCAGCTCGGTCCGCGCCTGATCGACAGC 
CAGAGTGCCGCGTTGGACTGCTGGGTCCGCGAATACCGTGGGCTGCTCGGCATCGCGCTGACCGACTGCATC 
ACCACCGATGCCTTCCTGCGCGACTTCGACCTCTATTTCGCCAAGCTCTTCGACGGCCTCCGCCACGACTCG 
GGCGACCCGTTGCTGTGGGCGGAAAAGACCATCGCCCACTACCTCAAGCTGGGCATCGACCCGCTGACCAAG 
ACCCTGGTGTTCTCCGACGGCCTCGACCTGCCGCGGGCCCTGAAGATCTATCGCGCGTTGCAGGGCCGGATC 
AACGTCAGCTTCGGCATAGGTACGCATTTCACCTGCGACCTGCCGGGCGTCGAGCCGATGAACATCGTGGTG 
AAGATGAGCGCCTGCAACGGCCATCCGGTGGCGAAGATCTCCGATACGCCCGGCAAGGCGCAATGCCGGGAC 
CCTGACTTCATCCACTACCTCAAGCACGTGTTCCAGGTCGCCTAG