ViewGene
Locus Name:
GeneID:
Location:

GeneID:1408
TrivialName: PA14_12010
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_12010
PAO1 Ortholog Locus:PA4007
Sequence Length:1266
Protein Length:421
Start: 1038138
Stop: 1039403
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG07394.1|AE004817_18 (AE004817) gamma-glutamyl phosphate reductase [Pseudomonas aeruginosa PAO1]
Identities = 420/421 (99%), Positives = 420/421 (99%)
Sequence:
ATGACCGAGTCCGTCCTCGACTATATGAGCCGCCTGGGCCGCGATGCCCGCGCCGCCTCGCGGTTGCTCGCG
CGCGCCGCCACCGCGCAGAAGAACCGCGCCCTGCTGGCCGCGGCCGATGCGCTGGACGCCGCCCGCGCGGAG
TTGTCCCACGCCAACGAGCAGGACCTCGCCGCCGGCCGCGCCAATGGCCTGGAGCCGGCGATGCTGGACCGC
TTGGCGCTGACCCCGGCGCGCATCGACGACATGATCGAGGGCCTGCGCCAGGTCGCCACGCTGCCCGACCCG
ATCGGCGAGATCCGCGACATGCGCTATGTGCCCTCGGGCATCCAGATCGGCAAGATGCGCGTGCCGCTGGGC
GTGGTCGGGATCATCTACGAGTCGCGGCCGAACGTGACCATCGACGCCGCCAGCCTGTGCCTGAAGTCCGGC
AACGCGACCATCCTGCGCGGCGGCTCCGAGGCGATCCACTCCAACCAGGCGATCGCCCGCTGCATCCAGCAG
GGCCTGGCCGAGGCCGGCCTGCCGGCCGCCGCCGTGCAGGTGGTGGAAACCACCGACCGCGCCGCGGTCGGC
GCGCTGATCAGCATGCCCGAATACGTCGACGTGATCGTCCCGCGCGGCGGCAAGGGCCTGATCGAGCGCATC
AGCCGCGAGGCCAAGGTGCCGGTGATCAAGCACCTGGACGGCATCTGCCACGTCTACATCGATGTCGCCGCC
GACCTGGACAAGGCGATTCGCGTCGCCGACAACGCCAAGACCCAGCGCTACGCGCCGTGCAACACCATGGAG
ACGCTGCTGGTACATGCCGGTATCGCCGAGCGGGCGCTGCCGCCGCTGGCCACGATCTACCGCGAGAAGGGC
GTGGAACTGCGCGGCGACGCCGCGACCCGTGCGCTGCTCGGCGCAGACGTGCTGGAGGCTACCGAGGAGGAC
TGGCGTACCGAGTACAACGCGCCGATCCTGTCGATCCGCATCGTCGACGGGCTGGACGCGGCGATCGAGCAC
ATCAACACTTACGGCTCGCAGCACACCGACGCGATCATTACCGAGAACTTCAGCGATGCGCGGCGTTTCCTC
GCCGAAGTGGATTCCGCTTCGGTGATGGTCAACGCCTCGACCCGCTTCGCCGACGGCTTCGAGTACGGCCTC
GGCGCCGAGATCGGGATTTCCACCGACAAGCTGCACGCCCGCGGCCCGGTCGGCCTGGAAGGCCTGACCAGC
GAGAAGTACGTCGTCTTCGGCGACGGTCACGTGCGCACCTGA
Translation:
MTESVLDYMSRLGRDARAASRLLARAATAQKNRALLAAADALDAARAELSHANEQDLAAGRANGLEPAMLDR
LALTPARIDDMIEGLRQVATLPDPIGEIRDMRYVPSGIQIGKMRVPLGVVGIIYESRPNVTIDAASLCLKSG
NATILRGGSEAIHSNQAIARCIQQGLAEAGLPAAAVQVVETTDRAAVGALISMPEYVDVIVPRGGKGLIERI
SREAKVPVIKHLDGICHVYIDVAADLDKAIRVADNAKTQRYAPCNTMETLLVHAGIAERALPPLATIYREKG
VELRGDAATRALLGADVLEATEEDWRTEYNAPILSIRIVDGLDAAIEHINTYGSQHTDAIITENFSDARRFL
AEVDSASVMVNASTRFADGFEYGLGAEIGISTDKLHARGPVGLEGLTSEKYVVFGDGHVRT*
AnnotationID:1417GeneID:1408
AnnotatorUID: danlee
Modification Date/Time: 2006-01-27 16:04:49
Gene Name:proA
Confidence Code:2
GeneProduct:probable gamma-glutamyl phosphate reductase
Cell Localization:(1) Cytoplasmic
Synonyms:proB
Cell Localization Confidence Code:3
MolecularFunction:
Functional Category:(2) Amino acid biosynthesis and metabolism
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:COG0014,KOG4165
Secondary Functional Category(ies):
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
gb|AAG07394.1|AE004817_18 (AE004817) gamma-glutamyl phosphate reductase [Pseudomonas aeruginosa PAO1]
Length = 421
Score =  812 bits (2098), Expect = 0.0
Identities = 420/421 (99%), Positives = 420/421 (99%)


dbj|BAB33693.1| (AP002551) gamma-glutamylphosphate reductase [Escherichia coli O157:H7]
Length: 424
PctIdentity: 47.16
PctSimilarity: 66.98
Gaps: 10
Score: 997.0






 
Structural Features:
gnl|CDD|9890 COG0014, ProA, Gamma-glutamyl phosphate reductase
[Amino acid transport and metabolism]
Length = 417
Score =  624 bits (1610), Expect = e-179
Identities = 265/417 (63%), Positives = 324/417 (77%)






   
Genomic Context:






   
Comment:


















    ReferenceID:2819




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:19964




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:34288




  
Author/Investigator(s):
        Hayzer DJ, Leisinger T.





  
Title:
        The gene-enzyme relationships of proline biosynthesis in Escherichia coli.




  
PubMed:
        6255065


MedLine:
        




    
Source:
J Gen Microbiol. 1980 Jun;118(2):287-93.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=6255065

















    ReferenceID:34289




  
Author/Investigator(s):
        Krishna RV, Beilstein P, Leisinger T.





  
Title:
        Biosynthesis of proline in Pseudomonas aeruginosa. Properties of gamma-glutamyl phosphate reductase and 1-pyrroline-5-carboxylate reductase.




  
PubMed:
        114173


MedLine:
        




    
Source:
Biochem J. 1979 Jul 1;181(1):223-30.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=114173









           
Homologs By Global Alignment 

           

       



     
Gene ID:1408
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
2560
                           PA4007_tr
                           translation of PA4007
                           421
                           99.76
                           99.76
                           0
                           2098.0
     

     
     
80878
                           gb|AAG07394.1|AE004817_18
                           (AE004817) gamma-glutamyl phosphate reductase [Pseudomonas aeruginosa PAO1]
                           421
                           99.76
                           99.76
                           0
                           2098.0
     

     
     
80879
                           gb|AAN70380.1|AE016679_12
                           (AE016792) gamma-glutamyl phosphate reductase [Pseudomonas putida KT2440]
                           425
                           76.94
                           85.17
                           6
                           1619.0
     

     
     
80880
                           gb|AAO58258.1|
                           (AE016873) gamma-glutamyl phosphate reductase [Pseudomonas syringae pv. tomato str. DC3000]
                           423
                           76.59
                           84.39
                           4
                           1599.0
     

     
     
80882
                           emb|CAD16448.1|
                           (AL646071) PROBABLE GAMMA-GLUTAMYL PHOSPHATE REDUCTASE (GPR)(GLUTAMATE-5-SEMIALDEHYDE DEHYDROGENASE) OXIDOREDUCTASE PROTEIN [Ralstonia solanacearum]
                           428
                           70.56
                           80.84
                           9
                           1480.0
     

     






      

          
GC ORFID: 44410How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA4007Glimmer Score: 

          
Start: 3915800Stop: 3914535

          
Length: 1266


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA4007 translation of PA4007Homolog Bit Score 812.0

          
Other Homologs:  psyr_15may02_Scaffold4_revised_gene5322 (ORF: BLASTX 621.0), t_PA4007 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGACCGAGTCCGTCCTCGACTATATGAGCCGCCTGGGCCGCGATGCCCGCGCCGCCTCGCGGTTGCTCGCG 
CGCGCCGCCACCGCGCAGAAGAACCGCGCCCTGCTGGCCGCGGCCGATGCGCTGGACGCCGCCCGCGCGGAG 
TTGTCCCACGCCAACGAGCAGGACCTCGCCGCCGGCCGCGCCAATGGCCTGGAGCCGGCGATGCTGGACCGC 
TTGGCGCTGACCCCGGCGCGCATCGACGACATGATCGAGGGCCTGCGCCAGGTCGCCACGCTGCCCGACCCG 
ATCGGCGAGATCCGCGACATGCGCTATGTGCCCTCGGGCATCCAGATCGGCAAGATGCGCGTGCCGCTGGGC 
GTGGTCGGGATCATCTACGAGTCGCGGCCGAACGTGACCATCGACGCCGCCAGCCTGTGCCTGAAGTCCGGC 
AACGCGACCATCCTGCGCGGCGGCTCCGAGGCGATCCACTCCAACCAGGCGATCGCCCGCTGCATCCAGCAG 
GGCCTGGCCGAGGCCGGCCTGCCGGCCGCCGCCGTGCAGGTGGTGGAAACCACCGACCGCGCCGCGGTCGGC 
GCGCTGATCAGCATGCCCGAATACGTCGACGTGATCGTCCCGCGCGGCGGCAAGGGCCTGATCGAGCGCATC 
AGCCGCGAGGCCAAGGTGCCGGTGATCAAGCACCTGGACGGCATCTGCCACGTCTACATCGATGTCGCCGCC 
GACCTGGACAAGGCGATTCGCGTCGCCGACAACGCCAAGACCCAGCGCTACGCGCCGTGCAACACCATGGAG 
ACGCTGCTGGTACATGCCGGTATCGCCGAGCGGGCGCTGCCGCCGCTGGCCACGATCTACCGCGAGAAGGGC 
GTGGAACTGCGCGGCGACGCCGCGACCCGTGCGCTGCTCGGCGCAGACGTGCTGGAGGCTACCGAGGAGGAC 
TGGCGTACCGAGTACAACGCGCCGATCCTGTCGATCCGCATCGTCGACGGGCTGGACGCGGCGATCGAGCAC 
ATCAACACTTACGGCTCGCAGCACACCGACGCGATCATTACCGAGAACTTCAGCGATGCGCGGCGTTTCCTC 
GCCGAAGTGGATTCCGCTTCGGTGATGGTCAACGCCTCGACCCGCTTCGCCGACGGCTTCGAGTACGGCCTC 
GGCGCCGAGATCGGGATTTCCACCGACAAGCTGCACGCCCGCGGCCCGGTCGGCCTGGAAGGCCTGACCAGC 
GAGAAGTACGTCGTCTTCGGCGACGGTCACGTGCGCACCTGA