ViewGene
Locus Name:
GeneID:
Location:

GeneID:1401
TrivialName: PA14_00450
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_00450
PAO1 Ortholog Locus:PA0036
Sequence Length:1209
Protein Length:402
Start: 39119
Stop: 37911
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG03426.1|AE004443_4 (AE004443) tryptophan synthase beta chain [Pseudomonas aeruginosa PAO1]
Identities = 400/402 (99%), Positives = 402/402 (100%)
Sequence:
ATGACTTCCTATCGCAACGGCCCCGACGCCAAGGGCCTGTTCGGCCGCTTCGGCGGCCAGTACGTCGCCGAG
ACCCTGATGCCGCTGATCCTCGACCTCGCCCGCGAGTACGAGAAGGCCAAGGACGACCCGGCGTTCCAGGAG
GAACTGGCCTACTTCCAGCGCGACTACGTCGGCCGGCCGAGCCCGCTGTACTTCGCCGAGCGCCTGACCGAG
CACTGCGGCGGGGCGAAGATCTACCTCAAGCGCGAGGAGCTGAACCATACCGGCGCGCACAAGATCAACAAC
TGCATCGGCCAGATCCTCCTGGCCCGGCGCATGGGCAAGAAACGCATCATCGCCGAGACCGGCGCCGGCATG
CACGGCGTGGCCACCGCCACCGTCGCCGCGCGCTTCGGCTTGCAGTGCGTGATCTACATGGGCACCACCGAC
ATCGACCGGCAGCAGGCCAACGTCTTCCGCATGAAGCTGCTGGGCGCCGAGGTGATCCCGGTGACCGCCGGC
ACCGGTACCCTGAAGGACGCCATGAACGAGGCGCTGCGCGATTGGGTGACCAACGTCGACAGCACCTTCTAC
CTGATCGGCACGGTCGCCGGCCCGCATCCGTACCCGGCGATGGTCCGTGACTTCCAGGCGGTGATCGGCAAG
GAAACCCGCGAGCAACTGGCCGAGAAGGAAGGGCGCCTGCCCAATTCGCTGATCGCCTGCATCGGCGGCGGC
TCCAACGCCATGGGCCTGTTCCACCCGTTCCTCGACGACGCCGGGGTGCAGATCGTCGGCGTGGAAGCCGCC
GGCCACGGCATCGACACCGGCAAGCACGCGGCCAGCCTGAACGGCGGGGTTCCCGGCGTGCTGCACGGCAAC
CGCACCTTCCTGCTGCAGGATGCGGATGGCCAGATCATCGACGCACACTCCATCTCCGCCGGCCTCGACTAT
CCCGGCATCGGCCCGGAACACGCCTGGCTGCACGACACCGGCCGCGTCGAGTACACCTCGATCACCGACGAC
GAAGCCCTGGAGGCCTTCCACACCTGCTGCCGCCTCGAAGGCATCATCCCGGCACTAGAAAGCTCCCATGCC
CTGGCCGAGGTCTTCAAGCGTGCGCCCAGCCTGCCCAAGGAGCACATCATGGTGGTGAACCTGTCCGGTCGC
GGCGACAAGGACATGCAGACCGTCATGCACCACATGCAACAGGAGTCGAAAGCATGA
Translation:
MTSYRNGPDAKGLFGRFGGQYVAETLMPLILDLAREYEKAKDDPAFQEELAYFQRDYVGRPSPLYFAERLTE
HCGGAKIYLKREELNHTGAHKINNCIGQILLARRMGKKRIIAETGAGMHGVATATVAARFGLQCVIYMGTTD
IDRQQANVFRMKLLGAEVIPVTAGTGTLKDAMNEALRDWVTNVDSTFYLIGTVAGPHPYPAMVRDFQAVIGK
ETREQLAEKEGRLPNSLIACIGGGSNAMGLFHPFLDDAGVQIVGVEAAGHGIDTGKHAASLNGGVPGVLHGN
RTFLLQDADGQIIDAHSISAGLDYPGIGPEHAWLHDTGRVEYTSITDDEALEAFHTCCRLEGIIPALESSHA
LAEVFKRAPSLPKEHIMVVNLSGRGDKDMQTVMHHMQQESKA*
AnnotationID:1410GeneID:1401
AnnotatorUID: diggins
Modification Date/Time: 2005-03-22 14:02:09
Gene Name:trpB
Confidence Code:1
GeneProduct:tryptophan synthase beta chain
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(2) Amino acid biosynthesis and metabolism
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:COG0133
Secondary Functional Category(ies):
EC Number:4.2.1.20
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAA26014.1| (M95710) tryptophan synthase beta subunit [Pseudomonas syringae]
          Length = 408

 Score =  747 bits (1929), Expect = 0.0
 Identities = 360/396 (90%), Positives = 381/396 (96%)

>gb|AAF61457.1|AF139661_2 (AF139661) tryptophan synthase beta subunit [Azospirillum
           brasilense]
          Length = 405

 Score =  607 bits (1566), Expect = e-173
 Identities = 293/396 (73%), Positives = 335/396 (84%), Gaps = 2/396 (0%)

>dbj|BAC46010.1| (AP005937) tryptophan synthase beta subunit [Bradyrhizobium
           japonicum USDA 110]
          Length = 405

 Score =  602 bits (1551), Expect = e-171
 Identities = 291/399 (72%), Positives = 339/399 (84%), Gaps = 1/399 (0%)








 
Structural Features:
COG0133, TrpB, Tryptophan synthase beta chain 
[Amino acid transport and metabolism]
CD-Length = 396 residues,  99.2% aligned
Score =  719 bits (1857), Expect = 0.0
Identities = 281/394 (71%), Positives = 330/394 (83%), Gaps = 1/394 (0%)








   
Genomic Context:






   
Comment:


















    ReferenceID:2805




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:19950




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:33399




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:1401_cdd.html


    		




 
URL:

















    ReferenceID:35515




  
Author/Investigator(s):
        Auerbach S, Gao J, Gussin GN.





  
Title:
        Nucleotide sequences of the trpI, trpB, and trpA genes of Pseudomonas syringae: positive control unique to fluorescent pseudomonads.




  
PubMed:
        8423001


MedLine:
        




    
Source:
Gene. 1993 Jan 15;123(1):25-32.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=8423001









           
Homologs By Global Alignment 

           

       



     
Gene ID:1401
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
2551
                           PA0036_tr
                           translation of PA0036
                           402
                           99.50
                           99.99
                           0
                           2103.0
     

     
     
80338
                           gb|AAG03426.1|AE004443_4
                           (AE004443) tryptophan synthase beta chain [Pseudomonas aeruginosa PAO1]
                           402
                           99.50
                           99.99
                           0
                           2103.0
     

     
     
80339
                           gb|AAA88462.1|
                           (M15826) tryptophan synthase beta subunit [Pseudomonas aeruginosa]
                           402
                           98.75
                           99.50
                           0
                           2087.0
     

     
     
80340
                           gb|AAA26014.1|
                           (M95710) tryptophan synthase beta subunit [Pseudomonas syringae]
                           408
                           88.23
                           93.62
                           6
                           1927.0
     

     
     
80342
                           emb|CAA31661.1|
                           (X13299) unnamed protein product [Pseudomonas putida]
                           405
                           83.20
                           90.86
                           3
                           1812.0
     

     






      

          
GC ORFID: 44403How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA0036Glimmer Score: 

          
Start: 5840456Stop: 5839248

          
Length: 1209


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA0036 translation of PA0036Homolog Bit Score 814.0

          
Other Homologs:  psyr_15may02_Scaffold2_revised_gene4087 (ORF: BLASTX 694.0), t_PA0036 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGACTTCCTATCGCAACGGCCCCGACGCCAAGGGCCTGTTCGGCCGCTTCGGCGGCCAGTACGTCGCCGAG 
ACCCTGATGCCGCTGATCCTCGACCTCGCCCGCGAGTACGAGAAGGCCAAGGACGACCCGGCGTTCCAGGAG 
GAACTGGCCTACTTCCAGCGCGACTACGTCGGCCGGCCGAGCCCGCTGTACTTCGCCGAGCGCCTGACCGAG 
CACTGCGGCGGGGCGAAGATCTACCTCAAGCGCGAGGAGCTGAACCATACCGGCGCGCACAAGATCAACAAC 
TGCATCGGCCAGATCCTCCTGGCCCGGCGCATGGGCAAGAAACGCATCATCGCCGAGACCGGCGCCGGCATG 
CACGGCGTGGCCACCGCCACCGTCGCCGCGCGCTTCGGCTTGCAGTGCGTGATCTACATGGGCACCACCGAC 
ATCGACCGGCAGCAGGCCAACGTCTTCCGCATGAAGCTGCTGGGCGCCGAGGTGATCCCGGTGACCGCCGGC 
ACCGGTACCCTGAAGGACGCCATGAACGAGGCGCTGCGCGATTGGGTGACCAACGTCGACAGCACCTTCTAC 
CTGATCGGCACGGTCGCCGGCCCGCATCCGTACCCGGCGATGGTCCGTGACTTCCAGGCGGTGATCGGCAAG 
GAAACCCGCGAGCAACTGGCCGAGAAGGAAGGGCGCCTGCCCAATTCGCTGATCGCCTGCATCGGCGGCGGC 
TCCAACGCCATGGGCCTGTTCCACCCGTTCCTCGACGACGCCGGGGTGCAGATCGTCGGCGTGGAAGCCGCC 
GGCCACGGCATCGACACCGGCAAGCACGCGGCCAGCCTGAACGGCGGGGTTCCCGGCGTGCTGCACGGCAAC 
CGCACCTTCCTGCTGCAGGATGCGGATGGCCAGATCATCGACGCACACTCCATCTCCGCCGGCCTCGACTAT 
CCCGGCATCGGCCCGGAACACGCCTGGCTGCACGACACCGGCCGCGTCGAGTACACCTCGATCACCGACGAC 
GAAGCCCTGGAGGCCTTCCACACCTGCTGCCGCCTCGAAGGCATCATCCCGGCACTAGAAAGCTCCCATGCC 
CTGGCCGAGGTCTTCAAGCGTGCGCCCAGCCTGCCCAAGGAGCACATCATGGTGGTGAACCTGTCCGGTCGC 
GGCGACAAGGACATGCAGACCGTCATGCACCACATGCAACAGGAGTCGAAAGCATGA