ViewGene

Locus Name:
GeneID:
Location:

GeneID: 14

TrivialName: PA14_09270

AnnotatorUID:

Modification Date/Time: 2006-04-06 13:01:23

LocusName: PA14_09270

PAO1 Ortholog Locus: PA4226

Sequence Length: 4317

Protein Length: 1438

Start: 785708

Stop: 790024

Strand: +

Type:

ChromosomeID: 2

Status: ACTIVE

Frame Discrepancy: FALSE

Missense Discrepancy: FALSE

Comments:
Homology: gb|AAD55800.1|AF184621_1 (AF184621) pyochelin synthetase PchE [Pseudomonas aeruginosa] Identities = 1438/1438 (100%), Positives = 1438/1438 (100%)

Sequence:

ATGGATCTGCCCCCCGATTCCCGTACCGCCCTGCGCGACTGGCTGACCGAGCAGCTCGCCGACCTGCTCGGC
GAACCGCTTGCCGACGTGCGCGCCCTGGCGGACGACGACGACCTGCTGGGCTGCGGCCTCGACTCGATCCGC
CTGATGTACCTGCAGGAACGCCTGCGCGCGCGTGGCTCGACGCTGGACTTCGCCCAGTTGGCGCAGCGCCCC
TGCCTGGGGGCCTGGCTCGACCTGCTGGCCTGCGCGGACCGGCTGTCCGCCCCGGCAACGGTCGCGCTGCCG
ACGGTGCAGGATCGCGACCAGCCGTTCGAGCTGTCTTCCGTGCAGCAGGCCTACTGGCTGGGACGTGGCGCC
GGCGAGGTGCTGGGCAACGTCAGCTGCCATGCCTTCCTGGAATTCCGCACGCGGGATGTCGACCCGCAGCGC
CTGGCCGCGGCGGCGGAGTGCGTGCGTCAACGCCACCCGATGTTGCGGGCGCGCTTCTTCGACGGTCGCCAG
CAGATCCTTCCGACGCCGCCGCTGTCCTGCTTCGACCTGCAGGACTGGCGTACCTTGCAGGTGGACGAGGCC
GAGCGCGACTGGCAGGCGCTGCGCGACTGGCGCGCCCATGAATGCCTGGCGGTGGAGCGCGGCCAGGTGTTC
CTGCTCGGGCTGGTGCGCATGCCGGGCGGCGAGGATCGCCTCTGGCTGAGTCTCGACCTGCTTGCCGCCGAT
GTCGAAAGCCTGCGCCTGCTGCTGGCCGAACTGGGCGTCGCCTACCTGGCGCCGGAGCGCCTGGCGGAGCCG
CCGGCGCTGCATTTCGCCGACTACCTGGCGCGCCGTGCGGCGCAACGCGCCGAGGCCGCGGCGCGGGCCCGC
GACTACTGGCTGGAACGCCTGCCGCGCTTGCCGGACGCGCCGGCCTTGCCGTTGGCCTGCGCGCCGGAAAGC
ATCCGCCAGCCGCGCACCCGGCGCCTGGCATTCCAGCTTTCCGCCGGCGAGAGCCGGCGCCTGGAGCGTCTT
GCCGCGCAGCATGGCGTGACCTTGTCCAGCGTGTTCGGCTGCGCCTTCGCGCTGGTCCTGGCGCGCTGGAGC
GAAAGCGCGGAATTTCTCCTCAACGTGCCGTTGTTCGATCGGCATGCCGACGACCCGCGTATCGGCGAGGTG
ATCGCCGACTTCACCACCCTGTTGCTGCTGGAGTGCCGGATGCAGGCCGGGGTGTCCTTCGCCGAGGCGGTG
AAGAGCTTCCAGCGCAACCTCCACGGAGCCATCGACCACGCCGCATTCCCCGCCCTGGAGGTGCTCCGCGAG
GCGCGCCGGCAGGGCCAGCCGCGCTCGGCGCCGGTGGTGTTCGCCAGCAACCTGGGCGAGGAGGGCTTCGTC
CCGGCAGCCTTCCGCGACGCTTTCGGCGATCTCCACGACATGCTCTCGCAGACCCCGCAGGTCTGGCTCGAC
CACCAGCTCTACCGGGTGGGCGACGGTATCCTGCTGGCCTGGGACAGCGTCGTCGGCCTGTTTCCCGAAGGC
CTGCCGGAAACCATGTTCGAAGCCTACGTGGGGCTGCTCCAGCGTCTCTGCGACAGCACCTGGGAGCAGCCT
GCCGATCTGCCGTTGCCCTGGGCGCAGCAGGCGCGCCGGGCTCTGCTCAACGGCCAGCCGGCATGCGCCACG
GCGCGCACCCTGCATCGCGACTTCTTCCTTCGCGCCGCCGAGGCGCCGGATGCCGACGCGCTGCTCTATCGC
GACCAACGTGTCACCCGCGGCGAACTGGCCGAGCGTGCGCTGCGCATCGCCGGCGGCCTGCGCGAAGCCGGG
GTGCGCCCTGGCGACGCGGTCGAGGTCAGCCTGCCGCGCGGACCGCAGCAGGTCGCGGCGGTATTCGGCGTG
CTCGCCGCAGGCGCCTGCTACGTGCCGCTGGACATCGACCAGCCGCCCGCACGGCGGCGCCTGATCGAAGAG
GCCGCCGGGGTATGCCTGGCGATCACCGAGGAGGACGATCCGCAGGCCTTGCCGCCGCGCCTGGATGTCCAG
CGCCTGCTGCGCGGCCCGGCGCTGGCCGCCCCCGTGCCGCTGGCGCCGCAGGCGAGCGCCTATGTGATCTAC
ACCTCGGGCTCCACCGGGGTGCCCAAGGGCGTCGAGGTCAGCCACGCGGCGGCGATCAATACCATCGACGCG
CTGCTCGACCTGCTGCGGGTGGACGCAGCGGACCGCTTGCTGGCGGTCTCCGCGCTGGACTTCGATCTATCG
GTCTTCGACCTGTTCGGCGGCCTCGGCGCCGGTGCCAGCCTGGTCCTGCCGGCCCAGGAACAGGCGCGCGAT
GCCGCTGCCTGGGCGGAGGCTATCCAGCGGCATGCGGTGAGCCTGTGGAACTCGGCGCCGGCCTTGCTGGAG
ATGGCCCTCAGCCTGCCGGCGAGCCAGGCCGACTATCGCAGTCTGCGGGCGGTGCTGCTGTCCGGCGACTGG
GTGGCCCTGGACCTACCCGGCCGCCTGCGCCCGCGTTGTGCCGAAGGCTGCCGCCTGCATGTGCTGGGTGGC
GCTACCGAAGCGGGCATCTGGTCGAACCTGCAGAGCGTCGATACGGTGCCGCCGCACTGGCGCTCGATTCCC
TACGGCCGGCCATTGCCGGGACAGGCCTACCGGGTGGTCGACGCCCACGGGCGCGACGTGCCGGACCTGGTG
GTCGGTGAGCTGTGGATCGGCGGCGCCAGCCTGGCCCGCGGTTATCGCAACGATCCCGAACTCAGCGCCCGG
CGCTTCGTCCACGATGCCCAGGGCCGCTGGTATCGCACCGGCGATCGCGGTCGCTACTGGGACGACGGCACC
CTGGAATTCCTCGGCCGGGTCGACCAGCAGGTGAAAGTGCGCGGCCAGCGCATCGAGTTGGGCGAGGTGGAG
GCCGCGCTGTGCGCCCAGGCTGGCGTGGAGAGCGCCTGCGCGGCGGTGCTCGGCGGTGGCGTGGCGAGCCTC
GGCGCGGTGCTGGTACCGCGCCTGGCGCCACGGGCCGAAGGCTCCATGGAACTGCCGGCCGCACAGCCCTTC
GCCGGCCTGGCAGAGGCCGAGGCGGTACTCACCCGGGAAATCCTCGGCGCGCTGCTGGAGGCGCCGCTGGAG
CTAGACGACGGTTTGCGCCGGCGCTGGCTGGACTGGCTGGCGGACTCCGCCGCCAGCGCGCTGCCGCCGCTC
GACGAGGCGTTGCGCCGGCTCGGCTGGCAGGCCGCGGGGCTGACCGCGATGGGCAACGCTCTGCGCGGCCTG
CTCGCCGGCGAACAGGCGCCGGCCGCGCTGCTCCTCGATCCCTGGCTGGCGCCGCAGGCGGTGGCCGCGCGC
CTGCCGGACGGCCGCGAGGCCCTGGCGCGCCTGCTCGAAGCGCTGCCGACGCCGGCTGCCGGCGAACGCCTG
CGGGTGGCGGTGCTGGATACCCGCGCCGGGCTCTGGCTCGACCAGGGCATGGCCTCGCTGTTGCGCCCGGGG
TTGGAACTGACCCTCTTCGAACGCAGCCGCGTCCTCCTCGACGCCGCCGCCACCCGCTTGCCGGAACGGATC
GTGGTGCAGGCGCTGGACGACGGCCTGCTACCTGCCGAGCACCTCGGTCGCTACGACCGGGTGATCAGCTTC
GCCGCGCTGCACGCCTACGCGGCCAGCCGCGAAGGCCTGGCGCTGGCGGCGGCGCTGCTGCGCCCGCAGGGC
CGCCTGTTGCTGGTGGACCTGCTATGCGAGTCGCCACTGGCGCTGCTCGGTGCGGCCTTGCTCGACGACCGG
CCGCTGCGCCTGGCGGAGCTGCCGAGCCTGTTGGCCGATCTCGCCGCTGCGGGATTGGCGCCGCGTTGCCTG
TGGCGCAGCGAGCGGATCGCCCTGGTCGAGGCGCTGGCGCCGGGGCTCGGTCTCGACGCCGCCGCGCTCCAG
GCCGGTCTGGAGCAACGCCTGCCCCAGGCGATGCGGCCCGAACGCCTGTGGTGCCTGCCAAGCCTGCCGTTG
AACGGCAATGGCAAGGTCGATCGTCGCCGCCTGGCCGAGAGCATGACCCGCGCACTCGGCGAGTGTCGTGAC
GAGCCCTCGGCGGAGGAGCCGCTGGAAGCCCATGAGCAGGCGCTGGCCGAGTGCTGGGAAGCGGTCCTCAAA
CGCCCGGTGCGTCGTCGCGAGGCGAGCTTCTTCAGCCTCGGCGGCGACAGCCTGCTGGCGACCCGCCTGCTG
GCCGGCATACGTGAGCGTTTCGGCGTACGCCTGGGCATGGCCGACTTCTATCGCCAGCCGACCCTGGCCGGG
CTTGCCCGCCACTTGCAGGCGCAGACCGTCGAGATCGAGGAAACCCAACTGGAGGAGGGCGTGCTATGA

Translation:

MDLPPDSRTALRDWLTEQLADLLGEPLADVRALADDDDLLGCGLDSIRLMYLQERLRARGSTLDFAQLAQRP
CLGAWLDLLACADRLSAPATVALPTVQDRDQPFELSSVQQAYWLGRGAGEVLGNVSCHAFLEFRTRDVDPQR
LAAAAECVRQRHPMLRARFFDGRQQILPTPPLSCFDLQDWRTLQVDEAERDWQALRDWRAHECLAVERGQVF
LLGLVRMPGGEDRLWLSLDLLAADVESLRLLLAELGVAYLAPERLAEPPALHFADYLARRAAQRAEAAARAR
DYWLERLPRLPDAPALPLACAPESIRQPRTRRLAFQLSAGESRRLERLAAQHGVTLSSVFGCAFALVLARWS
ESAEFLLNVPLFDRHADDPRIGEVIADFTTLLLLECRMQAGVSFAEAVKSFQRNLHGAIDHAAFPALEVLRE
ARRQGQPRSAPVVFASNLGEEGFVPAAFRDAFGDLHDMLSQTPQVWLDHQLYRVGDGILLAWDSVVGLFPEG
LPETMFEAYVGLLQRLCDSTWEQPADLPLPWAQQARRALLNGQPACATARTLHRDFFLRAAEAPDADALLYR
DQRVTRGELAERALRIAGGLREAGVRPGDAVEVSLPRGPQQVAAVFGVLAAGACYVPLDIDQPPARRRLIEE
AAGVCLAITEEDDPQALPPRLDVQRLLRGPALAAPVPLAPQASAYVIYTSGSTGVPKGVEVSHAAAINTIDA
LLDLLRVDAADRLLAVSALDFDLSVFDLFGGLGAGASLVLPAQEQARDAAAWAEAIQRHAVSLWNSAPALLE
MALSLPASQADYRSLRAVLLSGDWVALDLPGRLRPRCAEGCRLHVLGGATEAGIWSNLQSVDTVPPHWRSIP
YGRPLPGQAYRVVDAHGRDVPDLVVGELWIGGASLARGYRNDPELSARRFVHDAQGRWYRTGDRGRYWDDGT
LEFLGRVDQQVKVRGQRIELGEVEAALCAQAGVESACAAVLGGGVASLGAVLVPRLAPRAEGSMELPAAQPF
AGLAEAEAVLTREILGALLEAPLELDDGLRRRWLDWLADSAASALPPLDEALRRLGWQAAGLTAMGNALRGL
LAGEQAPAALLLDPWLAPQAVAARLPDGREALARLLEALPTPAAGERLRVAVLDTRAGLWLDQGMASLLRPG
LELTLFERSRVLLDAAATRLPERIVVQALDDGLLPAEHLGRYDRVISFAALHAYAASREGLALAAALLRPQG
RLLLVDLLCESPLALLGAALLDDRPLRLAELPSLLADLAAAGLAPRCLWRSERIALVEALAPGLGLDAAALQ
AGLEQRLPQAMRPERLWCLPSLPLNGNGKVDRRRLAESMTRALGECRDEPSAEEPLEAHEQALAECWEAVLK
RPVRRREASFFSLGGDSLLATRLLAGIRERFGVRLGMADFYRQPTLAGLARHLQAQTVEIEETQLEEGVL*

AnnotationID:24 GeneID:14

AnnotatorUID: danlee

Modification Date/Time: 2003-11-12 13:01:05

Gene Name: pchE

Confidence Code: 1

GeneProduct: pyochelin synthetase

Cell Localization: ()

Synonyms: alt GeneProduct name: dihydroaeruginoic acid synthetase

Cell Localization Confidence Code: 5

MolecularFunction:

Functional Category: (27) Transport of small molecules

Alternate Gene Product Name:

Functional Category Confidence Code: 5

COGs:

Secondary Functional Category(ies):

EC Number:

Status: ACTIVE

Pathway:

Pyochelin synthesis

Homology:

identical to dihydroaeruginoic acid synthetase pchE (PA4226 in PAO1).

Structural Features:

Genomic Context:

Comment:

ReferenceID:31

Author/Investigator(s): GenNotator

Title:

PubMed:

MedLine:

Source:

Reference Type: BLASTP

Data:

URL:

ReferenceID:16928

Author/Investigator(s): Quadri,L.E., Keating,T.A., Patel,H.M. and Walsh,C.T.

Title: Assembly of the Pseudomonas aeruginosa nonribosomal peptide siderophore pyochelin: In vitro reconstitution of aryl-4,2-bisthiazoline synthetase activity from PchD, PchE, and PchF

PubMed: 10555976

MedLine: 20025424

Source:

Biochemistry 38 (45), 14941-14954 (1999)

Reference Type: Journal

Data:

URL:

ReferenceID:17173

Author/Investigator(s): GenNotator

Title: CDD Search

PubMed:

MedLine:

Source:

Reference Type: RPSBLAST

Data:

URL:

Homologs By Global Alignment

Gene ID:14

Identity:

10%

20%

30%

40%

50%

60%

70%

80%

90%

100%

HomologID Accession Description Length PctIdentity PctSimilarity Gaps Score

8566 gb|AAD55800.1|AF184621_1 (AF184621) pyochelin synthetase PchE [Pseudomonas aeruginosa] 1438 99.99 99.99 0 7356.0

25 PA4226_tr translation of PA4226 1438 99.02 99.23 0 7283.0

8567 gb|AAG07614.1|AE004839_7 (AE004839) dihydroaeruginoic acid synthetase [Pseudomonas aeruginosa PAO1] 1438 99.02 99.23 0 7283.0

8568 gb|AAC83656.1| (AF074705) dihydroaeruginoic acid synthetase; peptide synthetase [Pseudomonas aeruginosa] 1438 99.02 99.23 0 7283.0

8570 emb|CAD97265.1| (BX248342) PHENYLOXAZOLINE SYNTHASE MBTB (PHENYLOXAZOLINE SYNTHETASE) [Mycobacterium bovis subsp. bovis AF2122/97] 1569 34.16 45.82 286 1785.0

GC ORFID: `43016`	How Found: `BLASTX`
GC_TrimmedSeqID: `69`	Blast Result ID
Subject Sequence Name: `t_PA4226`	Glimmer Score:
Start: `4168229`	Stop: `4163913`
Length: `4317`
Start Codon: `ATG`	Truncated Start:
Stop Codon: `TGA`	Truncated Stop:
Homolog: `t_PA4226 translation of PA4226`	Homolog Bit Score `2810.0`
Other Homologs: `t_PA4226 (ORF: GLIMMER)`
GC ORF Sequence
ATGGATCTGCCCCCCGATTCCCGTACCGCCCTGCGCGACTGGCTGACCGAGCAGCTCGCCGACCTGCTCGGC GAACCGCTTGCCGACGTGCGCGCCCTGGCGGACGACGACGACCTGCTGGGCTGCGGCCTCGACTCGATCCGC CTGATGTACCTGCAGGAACGCCTGCGCGCGCGTGGCTCGACGCTGGACTTCGCCCAGTTGGCGCAGCGCCCC TGCCTGGGGGCCTGGCTCGACCTGCTGGCCTGCGCGGACCGGCTGTCCGCCCCGGCAACGGTCGCGCTGCCG ACGGTGCAGGATCGCGACCAGCCGTTCGAGCTGTCTTCCGTGCAGCAGGCCTACTGGCTGGGACGTGGCGCC GGCGAGGTGCTGGGCAACGTCAGCTGCCATGCCTTCCTGGAATTCCGCACGCGGGATGTCGACCCGCAGCGC CTGGCCGCGGCGGCGGAGTGCGTGCGTCAACGCCACCCGATGTTGCGGGCGCGCTTCTTCGACGGTCGCCAG CAGATCCTTCCGACGCCGCCGCTGTCCTGCTTCGACCTGCAGGACTGGCGTACCTTGCAGGTGGACGAGGCC GAGCGCGACTGGCAGGCGCTGCGCGACTGGCGCGCCCATGAATGCCTGGCGGTGGAGCGCGGCCAGGTGTTC CTGCTCGGGCTGGTGCGCATGCCGGGCGGCGAGGATCGCCTCTGGCTGAGTCTCGACCTGCTTGCCGCCGAT GTCGAAAGCCTGCGCCTGCTGCTGGCCGAACTGGGCGTCGCCTACCTGGCGCCGGAGCGCCTGGCGGAGCCG CCGGCGCTGCATTTCGCCGACTACCTGGCGCGCCGTGCGGCGCAACGCGCCGAGGCCGCGGCGCGGGCCCGC GACTACTGGCTGGAACGCCTGCCGCGCTTGCCGGACGCGCCGGCCTTGCCGTTGGCCTGCGCGCCGGAAAGC ATCCGCCAGCCGCGCACCCGGCGCCTGGCATTCCAGCTTTCCGCCGGCGAGAGCCGGCGCCTGGAGCGTCTT GCCGCGCAGCATGGCGTGACCTTGTCCAGCGTGTTCGGCTGCGCCTTCGCGCTGGTCCTGGCGCGCTGGAGC GAAAGCGCGGAATTTCTCCTCAACGTGCCGTTGTTCGATCGGCATGCCGACGACCCGCGTATCGGCGAGGTG ATCGCCGACTTCACCACCCTGTTGCTGCTGGAGTGCCGGATGCAGGCCGGGGTGTCCTTCGCCGAGGCGGTG AAGAGCTTCCAGCGCAACCTCCACGGAGCCATCGACCACGCCGCATTCCCCGCCCTGGAGGTGCTCCGCGAG GCGCGCCGGCAGGGCCAGCCGCGCTCGGCGCCGGTGGTGTTCGCCAGCAACCTGGGCGAGGAGGGCTTCGTC CCGGCAGCCTTCCGCGACGCTTTCGGCGATCTCCACGACATGCTCTCGCAGACCCCGCAGGTCTGGCTCGAC CACCAGCTCTACCGGGTGGGCGACGGTATCCTGCTGGCCTGGGACAGCGTCGTCGGCCTGTTTCCCGAAGGC CTGCCGGAAACCATGTTCGAAGCCTACGTGGGGCTGCTCCAGCGTCTCTGCGACAGCACCTGGGAGCAGCCT GCCGATCTGCCGTTGCCCTGGGCGCAGCAGGCGCGCCGGGCTCTGCTCAACGGCCAGCCGGCATGCGCCACG GCGCGCACCCTGCATCGCGACTTCTTCCTTCGCGCCGCCGAGGCGCCGGATGCCGACGCGCTGCTCTATCGC GACCAACGTGTCACCCGCGGCGAACTGGCCGAGCGTGCGCTGCGCATCGCCGGCGGCCTGCGCGAAGCCGGG GTGCGCCCTGGCGACGCGGTCGAGGTCAGCCTGCCGCGCGGACCGCAGCAGGTCGCGGCGGTATTCGGCGTG CTCGCCGCAGGCGCCTGCTACGTGCCGCTGGACATCGACCAGCCGCCCGCACGGCGGCGCCTGATCGAAGAG GCCGCCGGGGTATGCCTGGCGATCACCGAGGAGGACGATCCGCAGGCCTTGCCGCCGCGCCTGGATGTCCAG CGCCTGCTGCGCGGCCCGGCGCTGGCCGCCCCCGTGCCGCTGGCGCCGCAGGCGAGCGCCTATGTGATCTAC ACCTCGGGCTCCACCGGGGTGCCCAAGGGCGTCGAGGTCAGCCACGCGGCGGCGATCAATACCATCGACGCG CTGCTCGACCTGCTGCGGGTGGACGCAGCGGACCGCTTGCTGGCGGTCTCCGCGCTGGACTTCGATCTATCG GTCTTCGACCTGTTCGGCGGCCTCGGCGCCGGTGCCAGCCTGGTCCTGCCGGCCCAGGAACAGGCGCGCGAT GCCGCTGCCTGGGCGGAGGCTATCCAGCGGCATGCGGTGAGCCTGTGGAACTCGGCGCCGGCCTTGCTGGAG ATGGCCCTCAGCCTGCCGGCGAGCCAGGCCGACTATCGCAGTCTGCGGGCGGTGCTGCTGTCCGGCGACTGG GTGGCCCTGGACCTACCCGGCCGCCTGCGCCCGCGTTGTGCCGAAGGCTGCCGCCTGCATGTGCTGGGTGGC GCTACCGAAGCGGGCATCTGGTCGAACCTGCAGAGCGTCGATACGGTGCCGCCGCACTGGCGCTCGATTCCC TACGGCCGGCCATTGCCGGGACAGGCCTACCGGGTGGTCGACGCCCACGGGCGCGACGTGCCGGACCTGGTG GTCGGTGAGCTGTGGATCGGCGGCGCCAGCCTGGCCCGCGGTTATCGCAACGATCCCGAACTCAGCGCCCGG CGCTTCGTCCACGATGCCCAGGGCCGCTGGTATCGCACCGGCGATCGCGGTCGCTACTGGGACGACGGCACC CTGGAATTCCTCGGCCGGGTCGACCAGCAGGTGAAAGTGCGCGGCCAGCGCATCGAGTTGGGCGAGGTGGAG GCCGCGCTGTGCGCCCAGGCTGGCGTGGAGAGCGCCTGCGCGGCGGTGCTCGGCGGTGGCGTGGCGAGCCTC GGCGCGGTGCTGGTACCGCGCCTGGCGCCACGGGCCGAAGGCTCCATGGAACTGCCGGCCGCACAGCCCTTC GCCGGCCTGGCAGAGGCCGAGGCGGTACTCACCCGGGAAATCCTCGGCGCGCTGCTGGAGGCGCCGCTGGAG CTAGACGACGGTTTGCGCCGGCGCTGGCTGGACTGGCTGGCGGACTCCGCCGCCAGCGCGCTGCCGCCGCTC GACGAGGCGTTGCGCCGGCTCGGCTGGCAGGCCGCGGGGCTGACCGCGATGGGCAACGCTCTGCGCGGCCTG CTCGCCGGCGAACAGGCGCCGGCCGCGCTGCTCCTCGATCCCTGGCTGGCGCCGCAGGCGGTGGCCGCGCGC CTGCCGGACGGCCGCGAGGCCCTGGCGCGCCTGCTCGAAGCGCTGCCGACGCCGGCTGCCGGCGAACGCCTG CGGGTGGCGGTGCTGGATACCCGCGCCGGGCTCTGGCTCGACCAGGGCATGGCCTCGCTGTTGCGCCCGGGG TTGGAACTGACCCTCTTCGAACGCAGCCGCGTCCTCCTCGACGCCGCCGCCACCCGCTTGCCGGAACGGATC GTGGTGCAGGCGCTGGACGACGGCCTGCTACCTGCCGAGCACCTCGGTCGCTACGACCGGGTGATCAGCTTC GCCGCGCTGCACGCCTACGCGGCCAGCCGCGAAGGCCTGGCGCTGGCGGCGGCGCTGCTGCGCCCGCAGGGC CGCCTGTTGCTGGTGGACCTGCTATGCGAGTCGCCACTGGCGCTGCTCGGTGCGGCCTTGCTCGACGACCGG CCGCTGCGCCTGGCGGAGCTGCCGAGCCTGTTGGCCGATCTCGCCGCTGCGGGATTGGCGCCGCGTTGCCTG TGGCGCAGCGAGCGGATCGCCCTGGTCGAGGCGCTGGCGCCGGGGCTCGGTCTCGACGCCGCCGCGCTCCAG GCCGGTCTGGAGCAACGCCTGCCCCAGGCGATGCGGCCCGAACGCCTGTGGTGCCTGCCAAGCCTGCCGTTG AACGGCAATGGCAAGGTCGATCGTCGCCGCCTGGCCGAGAGCATGACCCGCGCACTCGGCGAGTGTCGTGAC GAGCCCTCGGCGGAGGAGCCGCTGGAAGCCCATGAGCAGGCGCTGGCCGAGTGCTGGGAAGCGGTCCTCAAA CGCCCGGTGCGTCGTCGCGAGGCGAGCTTCTTCAGCCTCGGCGGCGACAGCCTGCTGGCGACCCGCCTGCTG GCCGGCATACGTGAGCGTTTCGGCGTACGCCTGGGCATGGCCGACTTCTATCGCCAGCCGACCCTGGCCGGG CTTGCCCGCCACTTGCAGGCGCAGACCGTCGAGATCGAGGAAACCCAACTGGAGGAGGGCGTGCTATGA