ViewGene
Locus Name:
GeneID:
Location:

GeneID:1386
TrivialName: PA14_18740
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_18740
PAO1 Ortholog Locus:PA3525
Sequence Length:1218
Protein Length:405
Start: 1612257
Stop: 1613474
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG06913.1|AE004773_2 (AE004773) argininosuccinate synthase [Pseudomonas aeruginosa PAO1]
Identities = 405/405 (100%), Positives = 405/405 (100%)
Sequence:
ATGGCGGATGTGAAGAAGGTAGTCCTGGCGTATTCCGGCGGCCTGGATACCTCGGTAATTCTGAAGTGGTTG
CAAGATACCTATAACTGCGAAGTGGTGACCTTCACCGCCGACCTCGGCCAGGGCGAGGAAGTCGAACCGGCT
CGCGCCAAGGCCCGCGCGATGGGCGTCAAGGAAATCTACATCGACGACCTGCGCGAAGAATTCGTCCGCGAC
TTCGTCTACCCGATGTTCCGCGCCAACACCGTCTACGAAGGCGAGTACCTGCTGGGCACCTCCATCGCTCGT
CCGCTGATCGCCAAGCGCCTGATCGAGATCGCCAACGAGACCGGCGCCGACGCCATCTCCCACGGCGCCACC
GGCAAGGGCAACGACCAGGTCCGCTTCGAGCTGGGCGCCTACGCGCTGAAGCCCGGCGTCAAGGTGATCGCG
CCCTGGCGCGAGTGGGACCTGCTGTCCCGCGAGAAGCTGATGGACTATGCCGAAAAGCACGGCATCCCGATC
GAGCGCCACGGCAAGAAGAAGTCGCCGTACTCGATGGACGCCAACCTCCTGCATATCTCCTACGAAGGCGGC
GTGCTGGAAGATACCTGGACCGAGCACGAAGAAGACATGTGGAAGTGGACCGCTTCCCCGGAAAACGCGCCG
GACACCCCGACCTACATCGAGCTGACCTACCGCAAGGGCGACATCGTCGCCATCGACGGCAAGGACATGACC
CCGGCCGAGGTGCTCACCGAGCTGAACCGCGTCGGCGGGATCAACGGCATCGGCCGCCTCGACATCGTCGAG
AACCGCTATGTCGGCATGAAGTCCCGTGGCTGCTACGAGACCCCCGGCGGCACCATCATGCTCAAGGCCCAC
CGCGCCATCGAGTCGATCACCCTGGACCGCGAAGTGGCCCACCTGAAAGACGAGCTGATGCCCAAGTACGCC
AGCCTGATCTACACCGGCTACTGGTGGAGCCCGGAGCGCCTGATGCTGCAGCAGATGATCGACGCCTCGCAG
GTCAACGTGAACGGCGTAGTGCGCCTGAAGCTGTACAAGGGCAACGTCGTGGTGGTGGGCCGCAAGTCCGAC
GACTCGCTGTTCGACGCCAACATCGCGACCTTCGAGGAAGACGGCGGCGCCTACAACCAGGCCGATGCCGCG
GGCTTCATCAAGCTCAACGCGCTGCGCATGCGCATCGCCGCGAACAAGGGCCGCACGCTGTCCTGA
Translation:
MADVKKVVLAYSGGLDTSVILKWLQDTYNCEVVTFTADLGQGEEVEPARAKARAMGVKEIYIDDLREEFVRD
FVYPMFRANTVYEGEYLLGTSIARPLIAKRLIEIANETGADAISHGATGKGNDQVRFELGAYALKPGVKVIA
PWREWDLLSREKLMDYAEKHGIPIERHGKKKSPYSMDANLLHISYEGGVLEDTWTEHEEDMWKWTASPENAP
DTPTYIELTYRKGDIVAIDGKDMTPAEVLTELNRVGGINGIGRLDIVENRYVGMKSRGCYETPGGTIMLKAH
RAIESITLDREVAHLKDELMPKYASLIYTGYWWSPERLMLQQMIDASQVNVNGVVRLKLYKGNVVVVGRKSD
DSLFDANIATFEEDGGAYNQADAAGFIKLNALRMRIAANKGRTLS*
AnnotationID:1395GeneID:1386
AnnotatorUID: diggins
Modification Date/Time: 2005-04-19 16:04:52
Gene Name:argG
Confidence Code:2
GeneProduct:argininosuccinate synthase
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(2) Amino acid biosynthesis and metabolism
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:pfam00764,COG0137
Secondary Functional Category(ies):
EC Number:6.3.4.5
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAN66713.1|AE016299_9 (AE016778) argininosuccinate synthase [Pseudomonas putida KT2440]
          Length = 405

 Score =  782 bits (2019), Expect = 0.0
 Identities = 385/404 (95%), Positives = 394/404 (97%)

>emb|CAD85348.1| (BX321861) Argininosuccinate synthase [Nitrosomonas europaea ATCC
           19718]
          Length = 404

 Score =  640 bits (1650), Expect = 0.0
 Identities = 311/398 (78%), Positives = 352/398 (88%), Gaps = 1/398 (0%)

>gb|AAD36844.1|AE001816_1 (AE001816) argininosuccinate synthase [Thermotoga maritima]
          Length = 409

 Score =  383 bits (983), Expect = e-105
 Identities = 198/401 (49%), Positives = 277/401 (69%), Gaps = 13/401 (3%)






 
Structural Features:
pfam00764, Arginosuc_synth, Arginosuccinate synthase.
CD-Length = 390 residues, 100.0% aligned
Score =  475 bits (1224), Expect = 4e-135
Identities = 187/394 (47%), Positives = 269/394 (68%), Gaps = 5/394 (1%)

COG0137, ArgG, Argininosuccinate synthase 
[Amino acid transport and metabolism]
CD-Length = 403 residues, 100.0% aligned
Score =  595 bits (1537), Expect = 2e-171
Identities = 229/407 (56%), Positives = 301/407 (73%), Gaps = 6/407 (1%)







   
Genomic Context:






   
Comment:


















    ReferenceID:2775




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:19920




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:32041




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:1386_cdd.html


    		




 
URL:

















    ReferenceID:34114




  
Author/Investigator(s):
        Rodriguez-Garcia A, de la Fuente A, Perez-Redondo R, Martin JF, Liras P.





  
Title:
        Characterization and expression of the arginine biosynthesis gene cluster of Streptomyces clavuligerus.




  
PubMed:
        11075930


MedLine:
        




    
Source:
J Mol Microbiol Biotechnol. 2000 Oct;2(4):543-50.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=11075930

















    ReferenceID:34115




  
Author/Investigator(s):
        Ogawara H, Kasama H, Nashimoto K, Ohtsubo M, Higashi K, Urabe H.





  
Title:
        Cloning, sequence and expression of the argG gene from Streptomyces lavendulae.




  
PubMed:
        8449418


MedLine:
        




    
Source:
Gene. 1993 Mar 15;125(1):91-6.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=8449418

















    ReferenceID:34116




  
Author/Investigator(s):
        Haas D, Holloway BW, Schambock A, Leisinger T.





  
Title:
        The genetic organization of arginine biosynthesis in Pseudomonas aeruginosa.




  
PubMed:
        408599


MedLine:
        




    
Source:
Mol Gen Genet. 1977 Jul 7;154(1):7-22.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=408599









           
Homologs By Global Alignment 

           

       



     
Gene ID:1386
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
2523
                           PA3525_tr
                           translation of PA3525
                           405
                           99.99
                           99.99
                           0
                           2110.0
     

     
     
79946
                           gb|AAG06913.1|AE004773_2
                           (AE004773) argininosuccinate synthase [Pseudomonas aeruginosa PAO1]
                           405
                           99.99
                           99.99
                           0
                           2110.0
     

     
     
79947
                           gb|AAN66713.1|AE016299_9
                           (AE016778) argininosuccinate synthase [Pseudomonas putida KT2440]
                           405
                           95.06
                           97.28
                           0
                           2017.0
     

     
     
79948
                           gb|AAO57611.1|
                           (AE016870) argininosuccinate synthase [Pseudomonas syringae pv. tomato str. DC3000]
                           405
                           92.83
                           96.04
                           0
                           1971.0
     

     
     
79949
                           gb|AAQ59666.1|
                           (AE016917) argininosuccinate synthase [Chromobacterium violaceum ATCC 12472]
                           412
                           80.58
                           87.13
                           11
                           1709.0
     

     






      

          
GC ORFID: 44388How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA3525Glimmer Score: 

          
Start: 3341677Stop: 3340460

          
Length: 1218


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA3525 translation of PA3525Homolog Bit Score 817.0

          
Other Homologs:  psyr_15may02_Scaffold5_revised_gene6267 (ORF: BLASTX 763.0), t_PA3525 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGGCGGATGTGAAGAAGGTAGTCCTGGCGTATTCCGGCGGCCTGGATACCTCGGTAATTCTGAAGTGGTTG 
CAAGATACCTATAACTGCGAAGTGGTGACCTTCACCGCCGACCTCGGCCAGGGCGAGGAAGTCGAACCGGCT 
CGCGCCAAGGCCCGCGCGATGGGCGTCAAGGAAATCTACATCGACGACCTGCGCGAAGAATTCGTCCGCGAC 
TTCGTCTACCCGATGTTCCGCGCCAACACCGTCTACGAAGGCGAGTACCTGCTGGGCACCTCCATCGCTCGT 
CCGCTGATCGCCAAGCGCCTGATCGAGATCGCCAACGAGACCGGCGCCGACGCCATCTCCCACGGCGCCACC 
GGCAAGGGCAACGACCAGGTCCGCTTCGAGCTGGGCGCCTACGCGCTGAAGCCCGGCGTCAAGGTGATCGCG 
CCCTGGCGCGAGTGGGACCTGCTGTCCCGCGAGAAGCTGATGGACTATGCCGAAAAGCACGGCATCCCGATC 
GAGCGCCACGGCAAGAAGAAGTCGCCGTACTCGATGGACGCCAACCTCCTGCATATCTCCTACGAAGGCGGC 
GTGCTGGAAGATACCTGGACCGAGCACGAAGAAGACATGTGGAAGTGGACCGCTTCCCCGGAAAACGCGCCG 
GACACCCCGACCTACATCGAGCTGACCTACCGCAAGGGCGACATCGTCGCCATCGACGGCAAGGACATGACC 
CCGGCCGAGGTGCTCACCGAGCTGAACCGCGTCGGCGGGATCAACGGCATCGGCCGCCTCGACATCGTCGAG 
AACCGCTATGTCGGCATGAAGTCCCGTGGCTGCTACGAGACCCCCGGCGGCACCATCATGCTCAAGGCCCAC 
CGCGCCATCGAGTCGATCACCCTGGACCGCGAAGTGGCCCACCTGAAAGACGAGCTGATGCCCAAGTACGCC 
AGCCTGATCTACACCGGCTACTGGTGGAGCCCGGAGCGCCTGATGCTGCAGCAGATGATCGACGCCTCGCAG 
GTCAACGTGAACGGCGTAGTGCGCCTGAAGCTGTACAAGGGCAACGTCGTGGTGGTGGGCCGCAAGTCCGAC 
GACTCGCTGTTCGACGCCAACATCGCGACCTTCGAGGAAGACGGCGGCGCCTACAACCAGGCCGATGCCGCG 
GGCTTCATCAAGCTCAACGCGCTGCGCATGCGCATCGCCGCGAACAAGGGCCGCACGCTGTCCTGA