ViewGene
Locus Name:
GeneID:
Location:

GeneID:1377
TrivialName: PA14_46490
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_46490
PAO1 Ortholog Locus:PA1373
Sequence Length:1269
Protein Length:422
Start: 4137111
Stop: 4138379
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG04762.1|AE004567_1 (AE004567) 3-oxoacyl-acyl carrier protein synthase II [Pseudomonas aeruginosa PAO1]
Identities = 417/422 (98%), Positives = 420/422 (99%)
Sequence:
ATGAGCAAACGTATCGTCGTCACCGGCATGGGCGCGGTCAGCCCGCTGGGCTGCGGAGTCGAACCCATATGG
CAACGCCTGCTGGCCGGGCAATCGGGCATCGCCACGCTCCCGGCGGAGCTGATCGGCGACCTGCCCATCAGC
ATCGGCGGCCAGGTCCCCGACCGTGTCCAGGACCCGCAGGCCGGCTTCGATCCCGACCTGCTGCTGGCCGCC
AAGGAACAACGCAAGATGGACCGCTTCATCCTCTTCGCCCTGGCCGCCGCGCAAGAGGCCCTGGCCCAGGCC
GGCTGGGCGCCGCAGAGCGCCGAGGCGCAGGAGCGCACGGCGACCGTCATCGCCTCCGGGGTGGGCGGCTTC
CATGCCATCGCCGAAGCGGTGCGGACCACAGACGGCAAGGGACCGCGACGCCTGTCGCCCTTCACCATCCCG
TCCTTTCTCTGCAACATGGCGGCCGCCCATGTGTCGATCCGCCATGGCTTCAAGGGACCGCTGGGCGCCCCG
GTGACCGCCTGCGCGGCGGGCGTGCAAGCCATCGGCGACGCGGCGCGGATGATCCGCGCGGGAGAGGTCGAC
GTGGCGCTCTGCGGCGGCGCGGAAGCGACCGTCCACCGGGTCAGCCTGGCCGGCTTCGCCGCCGCGCGCGCC
CTGTCCAGCGATTTCAACGACAGCCCCGAACGCGCCTCGCGCCCCTTCGACCAGGCCCGCGACGGCTTCGTC
ATGGGCGAAGGCGCCGGCCTACTGGTCATCGAGGAACTGGAGCACGCCCTGGCGCGCGGCGCCAGGCCCATC
GCCGAGCTGGTGGGCTACGGCACCAGCGCCGACGCCTACCACATGACCGCCGGGCCGGAAGATGGCAGCGGT
GCCCGCCGGGCCATGCAGCAGGCGCTGCGCCAGGCCGGCGTGGAAGCGGCCGCGGTGCAGCACCTGAATGCC
CACGCCACCTCCACCCAGGTAGGCGACAAGGGCGAACTGGCCGCCATCAAGGCCGTGTTCGGCACCGGCAGC
GGGCTCGCCATCAGCGCGACCAAATCTGCCACCGGCCACCTCCTGGGCGCCGCCGGCGGCATCGAGGCGATC
TTCACGGTCCTGGCACTGCGCGACCAGCTCGCCCCCGCCACGCTCAACCTGGAAACGCCCGACACCGACGCC
GAGGGCCTCGACCTGGTTCGCGGCGAAGCCCAGCGCTGGCCGATGGAACACGCGCTGTCCAATGGCTTCGGC
TTCGGCGGGGTAAATGCCAGCCTGTTGTTCCGGCGTTGGGTGTAG
Translation:
MSKRIVVTGMGAVSPLGCGVEPIWQRLLAGQSGIATLPAELIGDLPISIGGQVPDRVQDPQAGFDPDLLLAA
KEQRKMDRFILFALAAAQEALAQAGWAPQSAEAQERTATVIASGVGGFHAIAEAVRTTDGKGPRRLSPFTIP
SFLCNMAAAHVSIRHGFKGPLGAPVTACAAGVQAIGDAARMIRAGEVDVALCGGAEATVHRVSLAGFAAARA
LSSDFNDSPERASRPFDQARDGFVMGEGAGLLVIEELEHALARGARPIAELVGYGTSADAYHMTAGPEDGSG
ARRAMQQALRQAGVEAAAVQHLNAHATSTQVGDKGELAAIKAVFGTGSGLAISATKSATGHLLGAAGGIEAI
FTVLALRDQLAPATLNLETPDTDAEGLDLVRGEAQRWPMEHALSNGFGFGGVNASLLFRRWV*
AnnotationID:1386GeneID:1377
AnnotatorUID: diggins
Modification Date/Time: 2005-04-27 09:09:22
Gene Name:fabF2
Confidence Code:2
GeneProduct:3-oxoacyl-acyl carrier protein synthase II
Cell Localization:()
Synonyms:fabF
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(13) Fatty acid and phospholipid metabolism
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:cd00834,COG0304
Secondary Functional Category(ies):secondary metabolites, biosynthesis, transport, and catabolism
EC Number:2.3.1.41
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAN68910.1|AE016522_7 (AE016786) 3-oxoacyl-(acyl-carrier-protein) synthase II, putative
           [Pseudomonas putida KT2440]
          Length = 437

 Score =  611 bits (1575), Expect = e-174
 Identities = 305/419 (72%), Positives = 354/419 (84%)

>dbj|BAC47794.1| (AP005944) 3-oxoacyl-(acyl-carrier-protein) synthase II
           [Bradyrhizobium japonicum USDA 110]
          Length = 420

 Score =  593 bits (1530), Expect = e-169
 Identities = 302/418 (72%), Positives = 339/418 (81%), Gaps = 1/418 (0%)

>gb|AAM86296.1|AE013877_4 (AE013877) 3-oxoacyl-[acyl-carrier-protein] synthase II [Yersinia
           pestis KIM]
          Length = 342

 Score =  481 bits (1237), Expect = e-135
 Identities = 238/321 (74%), Positives = 274/321 (85%)






 
Structural Features:
cd00834, KAS_I_II, Beta-ketoacyl-acyl carrier protein (ACP) synthase (KAS), 
type I and II. 
KASs are responsible for the elongation steps in fatty acid biosynthesis.
CD-Length = 406 residues, 100.0% aligned
Score =  481 bits (1240), Expect = 7e-137

COG0304, FabB, 3-oxoacyl-(acyl-carrier-protein) synthase 
[Lipid metabolism / Secondary metabolites biosynthesis, transport, and catabolism]
CD-Length = 412 residues,  99.8% aligned
Score =  435 bits (1120), Expect = 4e-123
Identities = 215/423 (50%), Positives = 273/423 (64%), Gaps = 14/423 (3%)






   
Genomic Context:






   
Comment:


















    ReferenceID:2757




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:19902




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:37398




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:1377_cdd.html


    		




 
URL:

















    ReferenceID:38005




  
Author/Investigator(s):
        Kutchma AJ, Hoang TT, Schweizer HP.





  
Title:
        Characterization of a Pseudomonas aeruginosa fatty acid biosynthetic gene cluster: purification of acyl carrier protein (ACP) and malonyl-coenzyme A:ACP transacylase (FabD).




  
PubMed:
        10464226


MedLine:
        




    
Source:
J Bacteriol. 1999 Sep;181(17):5498-504.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=10464226

















    ReferenceID:38006




  
Author/Investigator(s):
        Magnuson K, Carey MR, Cronan JE Jr.





  
Title:
        The putative fabJ gene of Escherichia coli fatty acid synthesis is the fabF gene.




  
PubMed:
        7768872


MedLine:
        




    
Source:
J Bacteriol. 1995 Jun;177(12):3593-5.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=7768872

















    ReferenceID:38007




  
Author/Investigator(s):
        Edwards P, Nelsen JS, Metz JG, Dehesh K.





  
Title:
        Cloning of the fabF gene in an expression vector and in vitro characterization of recombinant fabF and fabB encoded enzymes from Escherichia coli.




  
PubMed:
        9013860


MedLine:
        




    
Source:
FEBS Lett. 1997 Jan 27;402(1):62-6.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=9013860









           
Homologs By Global Alignment 

           

       



     
Gene ID:1377
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
2513
                           PA1373_tr
                           translation of PA1373
                           422
                           98.81
                           99.52
                           0
                           2116.0
     

     
     
79422
                           gb|AAG04762.1|AE004567_1
                           (AE004567) 3-oxoacyl-acyl carrier protein synthase II [Pseudomonas aeruginosa PAO1]
                           422
                           98.81
                           99.52
                           0
                           2116.0
     

     
     
79424
                           dbj|BAC47794.1|
                           (AP005944) 3-oxoacyl-(acyl-carrier-protein) synthase II [Bradyrhizobium japonicum USDA 110]
                           422
                           71.56
                           80.33
                           2
                           1527.0
     

     
     
79423
                           gb|AAN68910.1|AE016522_7
                           (AE016786) 3-oxoacyl-(acyl-carrier-protein) synthase II, putative [Pseudomonas putida KT2440]
                           437
                           69.79
                           81.00
                           15
                           1571.0
     

     
     
79425
                           dbj|BAB49860.1|
                           (AP003000) 3-oxoacyl-acyl carrier protein synthase II [Mesorhizobium loti]
                           434
                           67.74
                           80.41
                           13
                           1525.0
     

     






      

          
GC ORFID: 44379How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA1373Glimmer Score: 

          
Start: 816798Stop: 815530

          
Length: 1269


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TAGTruncated Stop: 

          
Homolog: t_PA1373 translation of PA1373Homolog Bit Score 819.0

          
Other Homologs:  t_PA2965 (ORF: BLASTX 383.0), psyr_15may02_Scaffold16_revised_gene2610 (ORF: BLASTX 366.0), t_PA1373 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGAGCAAACGTATCGTCGTCACCGGCATGGGCGCGGTCAGCCCGCTGGGCTGCGGAGTCGAACCCATATGG 
CAACGCCTGCTGGCCGGGCAATCGGGCATCGCCACGCTCCCGGCGGAGCTGATCGGCGACCTGCCCATCAGC 
ATCGGCGGCCAGGTCCCCGACCGTGTCCAGGACCCGCAGGCCGGCTTCGATCCCGACCTGCTGCTGGCCGCC 
AAGGAACAACGCAAGATGGACCGCTTCATCCTCTTCGCCCTGGCCGCCGCGCAAGAGGCCCTGGCCCAGGCC 
GGCTGGGCGCCGCAGAGCGCCGAGGCGCAGGAGCGCACGGCGACCGTCATCGCCTCCGGGGTGGGCGGCTTC 
CATGCCATCGCCGAAGCGGTGCGGACCACAGACGGCAAGGGACCGCGACGCCTGTCGCCCTTCACCATCCCG 
TCCTTTCTCTGCAACATGGCGGCCGCCCATGTGTCGATCCGCCATGGCTTCAAGGGACCGCTGGGCGCCCCG 
GTGACCGCCTGCGCGGCGGGCGTGCAAGCCATCGGCGACGCGGCGCGGATGATCCGCGCGGGAGAGGTCGAC 
GTGGCGCTCTGCGGCGGCGCGGAAGCGACCGTCCACCGGGTCAGCCTGGCCGGCTTCGCCGCCGCGCGCGCC 
CTGTCCAGCGATTTCAACGACAGCCCCGAACGCGCCTCGCGCCCCTTCGACCAGGCCCGCGACGGCTTCGTC 
ATGGGCGAAGGCGCCGGCCTACTGGTCATCGAGGAACTGGAGCACGCCCTGGCGCGCGGCGCCAGGCCCATC 
GCCGAGCTGGTGGGCTACGGCACCAGCGCCGACGCCTACCACATGACCGCCGGGCCGGAAGATGGCAGCGGT 
GCCCGCCGGGCCATGCAGCAGGCGCTGCGCCAGGCCGGCGTGGAAGCGGCCGCGGTGCAGCACCTGAATGCC 
CACGCCACCTCCACCCAGGTAGGCGACAAGGGCGAACTGGCCGCCATCAAGGCCGTGTTCGGCACCGGCAGC 
GGGCTCGCCATCAGCGCGACCAAATCTGCCACCGGCCACCTCCTGGGCGCCGCCGGCGGCATCGAGGCGATC 
TTCACGGTCCTGGCACTGCGCGACCAGCTCGCCCCCGCCACGCTCAACCTGGAAACGCCCGACACCGACGCC 
GAGGGCCTCGACCTGGTTCGCGGCGAAGCCCAGCGCTGGCCGATGGAACACGCGCTGTCCAATGGCTTCGGC 
TTCGGCGGGGTAAATGCCAGCCTGTTGTTCCGGCGTTGGGTGTAG