ViewGene
Locus Name:
GeneID:
Location:

GeneID:1376
TrivialName: PA14_20970
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_20970
PAO1 Ortholog Locus:PA3331
Sequence Length:1257
Protein Length:418
Start: 1806220
Stop: 1804964
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG06719.1|AE004755_6 (AE004755) cytochrome P450 [Pseudomonas aeruginosa PAO1]
Identities = 416/418 (99%), Positives = 416/418 (99%)
Sequence:
GTGCCTGATCGCAAACTGAGACTGGGCGAGGAACTGATCTCGCCACTGCACGCGCTCTACGACGGCCTGCAG
GTGGACGGCGCGCCGCGTCCCGCGCATCGCGCCGCCGAGCATCCGGTGTGGGTGGTGACGCGCTACCGCGAC
GCGCGCAAGGTCCTCAACCATCCGGGCGTCCGCCGCGACGCCCGGCAGGCCGCCGAACTCTACGCGAAGCGT
ACCGGCAGCCCGCGCGCGGGGATCGGCGAGGGACTCAGCCACCACATGCTCAACCTCGACCCGCCGGACCAT
ACCCGCCTGCGCTCGCTGGTCGGCCGCGCCTTCACCCCGCGCCAGGTGGAACGCCTGCAACCGCATATAGAA
CGGATCACCGAGGAATTGCTGGACGCCATGGCCGGCCGCGAACAGGCCGACCTGATGGCCGACTTCGCGATC
CCGCTGACCATCGCGGTGATCTTCGAGCTGCTGGGCATTCCCGAGGCCGAGCGCGAACACGCCCGCCAGTCC
TGGGAGCGCCAGGCGGAACTGCTGTCGCCGGAGGAGGCCCAGGCCCTGGCCGATGCGCAGGTCGACTACCTG
CGCGTGCTGCTCGAGGCCAAGCGCCGGCAGCCGGCCGACGACGTCTACAGCGGGCTGGTGCAGGCCGCCGAC
GAGAGCGGCCAACTGAGCGAGGCGGAACTCGTCTCCATGGCCCACCTGCTGATGATGAGCGGCTTCGAGACC
ACCATGAACATGATCGGCAACGCGCTGGTCACCCTGCTGGTCAACCCGGAGCAACTGGCGTTGCTGCGGGCG
CAGCCGGAACTCCTGCCCAACGCCATGGAAGAACTGGTCCGCCACGACAGCCCGGTGCGCGCCTCGATGTTG
CGCTTCACCGTGGAAGACGTAGAACTGGACGGGGTCACCATTCCCGCCGGCGAATACATCCTGGTCTCCAAC
CTGACCGCCAACCACGACGCCGAGCGCTTCGGCGATCCCGACCGCCTCGACCTCACCCGCAACACCGATGGC
CATCTCGGCTACGGCTTCGGCGTGCACTACTGCGTCGGCGCCTCGCTGGCCCGGCTGGAGGGGCGGATCGCC
ATCCAGCGCCTGCTCGCGCGCTTCCCCGACCTCCAGTTGGCGGTGCCCCACGCGGAACTGCAATGGCTGCCG
ATCACCTTCCTCCGAGCCCTGATCAGCGTGCCGGTGCGCACCGGATGCAGCGCCCCGGCGAACACCGCCTCC
CACGCCAACCCGATCGAGAGGATCGCCCAATGA
Translation:
MPDRKLRLGEELISPLHALYDGLQVDGAPRPAHRAAEHPVWVVTRYRDARKVLNHPGVRRDARQAAELYAKR
TGSPRAGIGEGLSHHMLNLDPPDHTRLRSLVGRAFTPRQVERLQPHIERITEELLDAMAGREQADLMADFAI
PLTIAVIFELLGIPEAEREHARQSWERQAELLSPEEAQALADAQVDYLRVLLEAKRRQPADDVYSGLVQAAD
ESGQLSEAELVSMAHLLMMSGFETTMNMIGNALVTLLVNPEQLALLRAQPELLPNAMEELVRHDSPVRASML
RFTVEDVELDGVTIPAGEYILVSNLTANHDAERFGDPDRLDLTRNTDGHLGYGFGVHYCVGASLARLEGRIA
IQRLLARFPDLQLAVPHAELQWLPITFLRALISVPVRTGCSAPANTASHANPIERIAQ*
AnnotationID:1385GeneID:1376
AnnotatorUID: diggins
Modification Date/Time: 2005-04-15 09:09:50
Gene Name:cyp23
Confidence Code:2
GeneProduct:cytochrome P450
Cell Localization:()
Synonyms:picK
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(1) Adaptation, protection
Alternate Gene Product Name:cytochrome P450 monooxygenase
Functional Category Confidence Code:5
COGs:COG2124,pfam00067
Secondary Functional Category(ies):biosynthesiis of cofactors, prosthetic groups and carriers
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAA26483.1| (M83110) cytochrome P-450 [Saccharopolyspora erythraea]
          Length = 405

 Score =  333 bits (854), Expect = 1e-90
 Identities = 173/362 (47%), Positives = 238/362 (65%), Gaps = 4/362 (1%)

>gb|AAC64105.1| (AF087022) cytochrome P450 monooxygenase [Streptomyces venezuelae]
          Length = 416

 Score =  296 bits (757), Expect = 2e-79
 Identities = 171/396 (43%), Positives = 236/396 (59%), Gaps = 17/396 (4%)






 
Structural Features:
COG2124, CypX, Cytochrome P450 
[Secondary metabolites biosynthesis, transport, and catabolism]
CD-Length = 411 residues,  87.8% aligned
Score =  260 bits (665), Expect = 3e-70
Identities = 148/371 (39%), Positives = 195/371 (52%), Gaps = 21/371 (5%)

pfam00067, p450, Cytochrome 
CD-Length = 461 residues,  84.2% aligned
Score =  103 bits (257), Expect = 6e-23
Identities = 79/395 (20%), Positives = 136/395 (34%), Gaps = 71/395 (17%)






   
Genomic Context:






   
Comment:


















    ReferenceID:14341




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:19900




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:32233




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:1376_cdd.html


    		




 
URL:

















    ReferenceID:34023




  
Author/Investigator(s):
        Trigui M, Pulvin S, Truffaut N, Thomas D, Poupin P.





  
Title:
        Molecular cloning, nucleotide sequencing and expression of genes encoding a cytochrome P450 system involved in secondary amine utilization in Mycobacterium sp. strain RP1.




  
PubMed:
        14759702


MedLine:
        




    
Source:
Res Microbiol. 2004 Jan-Feb;155(1):1-9.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=14759702

















    ReferenceID:34024




  
Author/Investigator(s):
        McLean KJ, Cheesman MR, Rivers SL, Richmond A, Leys D, Chapman SK, Reid GA, Price NC, Kelly SM, Clarkson J, Smith WE, Munro AW.





  
Title:
        Expression, purification and spectroscopic characterization of the cytochrome P450 CYP121 from Mycobacterium tuberculosis.




  
PubMed:
        12237220


MedLine:
        




    
Source:
J Inorg Biochem. 2002 Sep 20;91(4):527-41.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=12237220

















    ReferenceID:34025




  
Author/Investigator(s):
        Nelson DR.





  
Title:
        Cytochrome P450 and the individuality of species.




  
PubMed:
        10462435


MedLine:
        




    
Source:
Arch Biochem Biophys. 1999 Sep 1;369(1):1-10.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=10462435

















    ReferenceID:34026




  
Author/Investigator(s):
        Nebert DW, Jones JE, Owens J, Puga A.





  
Title:
        Evolution of the P450 gene superfamily.




  
PubMed:
        3406038


MedLine:
        




    
Source:
Prog Clin Biol Res. 1988;274:557-76.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=3406038









           
Homologs By Global Alignment 

           

       



     
Gene ID:1376
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
2512
                           PA3331_tr
                           translation of PA3331
                           418
                           99.52
                           99.52
                           0
                           2119.0
     

     
     
79400
                           gb|AAG06719.1|AE004755_6
                           (AE004755) cytochrome P450 [Pseudomonas aeruginosa PAO1]
                           418
                           99.28
                           99.52
                           0
                           2116.0
     

     
     
79401
                           gb|AAA26483.1|
                           (M83110) cytochrome P-450 [Saccharopolyspora erythraea]
                           432
                           42.82
                           58.33
                           41
                           867.0
     

     
     
79408
                           emb|CAC10308.1|
                           (AL939117) putative cytochrome P450 oxidoreductase [Streptomyces coelicolor A3(2)]
                           428
                           40.65
                           54.43
                           44
                           720.0
     

     
     
79405
                           dbj|BAC73960.1|
                           (AP005046) putative cytochrome P450 [Streptomyces avermitilis MA-4680]
                           428
                           40.42
                           56.77
                           50
                           745.0
     

     






      

          
GC ORFID: 44378How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA3331Glimmer Score: 

          
Start: 3147712Stop: 3148968

          
Length: 1257


          
Start Codon: GTGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA3331 translation of PA3331Homolog Bit Score 820.0

          
Other Homologs:  t_PA3331 (ORF: GLIMMER)

          
GC ORF Sequence

           
GTGCCTGATCGCAAACTGAGACTGGGCGAGGAACTGATCTCGCCACTGCACGCGCTCTACGACGGCCTGCAG 
GTGGACGGCGCGCCGCGTCCCGCGCATCGCGCCGCCGAGCATCCGGTGTGGGTGGTGACGCGCTACCGCGAC 
GCGCGCAAGGTCCTCAACCATCCGGGCGTCCGCCGCGACGCCCGGCAGGCCGCCGAACTCTACGCGAAGCGT 
ACCGGCAGCCCGCGCGCGGGGATCGGCGAGGGACTCAGCCACCACATGCTCAACCTCGACCCGCCGGACCAT 
ACCCGCCTGCGCTCGCTGGTCGGCCGCGCCTTCACCCCGCGCCAGGTGGAACGCCTGCAACCGCATATAGAA 
CGGATCACCGAGGAATTGCTGGACGCCATGGCCGGCCGCGAACAGGCCGACCTGATGGCCGACTTCGCGATC 
CCGCTGACCATCGCGGTGATCTTCGAGCTGCTGGGCATTCCCGAGGCCGAGCGCGAACACGCCCGCCAGTCC 
TGGGAGCGCCAGGCGGAACTGCTGTCGCCGGAGGAGGCCCAGGCCCTGGCCGATGCGCAGGTCGACTACCTG 
CGCGTGCTGCTCGAGGCCAAGCGCCGGCAGCCGGCCGACGACGTCTACAGCGGGCTGGTGCAGGCCGCCGAC 
GAGAGCGGCCAACTGAGCGAGGCGGAACTCGTCTCCATGGCCCACCTGCTGATGATGAGCGGCTTCGAGACC 
ACCATGAACATGATCGGCAACGCGCTGGTCACCCTGCTGGTCAACCCGGAGCAACTGGCGTTGCTGCGGGCG 
CAGCCGGAACTCCTGCCCAACGCCATGGAAGAACTGGTCCGCCACGACAGCCCGGTGCGCGCCTCGATGTTG 
CGCTTCACCGTGGAAGACGTAGAACTGGACGGGGTCACCATTCCCGCCGGCGAATACATCCTGGTCTCCAAC 
CTGACCGCCAACCACGACGCCGAGCGCTTCGGCGATCCCGACCGCCTCGACCTCACCCGCAACACCGATGGC 
CATCTCGGCTACGGCTTCGGCGTGCACTACTGCGTCGGCGCCTCGCTGGCCCGGCTGGAGGGGCGGATCGCC 
ATCCAGCGCCTGCTCGCGCGCTTCCCCGACCTCCAGTTGGCGGTGCCCCACGCGGAACTGCAATGGCTGCCG 
ATCACCTTCCTCCGAGCCCTGATCAGCGTGCCGGTGCGCACCGGATGCAGCGCCCCGGCGAACACCGCCTCC 
CACGCCAACCCGATCGAGAGGATCGCCCAATGA