ViewGene
Locus Name:
GeneID:
Location:

GeneID:1371
TrivialName: PA14_57810
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_57810
PAO1 Ortholog Locus:PA4450
Sequence Length:1266
Protein Length:421
Start: 5149061
Stop: 5147796
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG07838.1|AE004859_7 (AE004859) UDP-N-acetylglucosamine 1-carboxyvinyltransferase [Pseudomonas aeruginosa PAO1]
Identities = 421/421 (100%), Positives = 421/421 (100%)
Sequence:
ATGGATAAACTGATTATTACCGGCGGTAACCGCCTCGATGGCGAAATCCGCATTTCCGGCGCGAAGAACTCG
GCGCTGCCGATCCTGGCCGCGACCCTGCTGGCCGATACTCCGGTCACCGTCTGCAACCTGCCGCACCTGCAC
GACATTACCACCATGATCGAACTGTTCGGCCGCATGGGCGTGCAGCCGATCATCGACGAGAAGCTCAACGTC
GAAGTCGACGCCAGCAGCATCAAGACCCTGGTCGCGCCGTACGAACTGGTGAAGACCATGCGTGCCTCGATC
CTGGTGCTGGGTCCGATGCTGGCGCGCTTCGGCGAGGCCGAAGTGGCCCTGCCGGGCGGTTGCGCGATCGGT
TCGCGTCCGGTCGACCTGCATATCCGCGGTCTCGAGGCCATGGGCGCGCAGATCGAGGTCGAAGGCGGCTAC
ATCAAGGCCAAGGCGCCCGCCGGCGGCCTGCGTGGCGGTCACTTCTTCTTCGATACCGTCAGCGTGACCGGC
ACCGAGAACCTGATGATGGCCGCCGCGCTGGCCAACGGCCGCACCGTGCTGCAGAACGCCGCTCGCGAGCCG
GAGGTGGTCGACCTGGCCAACTGCCTGAACGCCATGGGCGCCAACGTCCAGGGCGCTGGCTCCGATACCATC
GTCATCGAAGGCGTGAAGCGCCTCGGCGGCGCTCGCTACGACGTACTGCCCGACCGTATCGAGACCGGCACC
TACCTGGTGGCCGCGGCCGCGACCGGCGGCCGGGTGAAGTTGAAGGATACCGATCCGACCATCCTCGAGGCG
GTCCTGCAGAAGCTGGAAGAGGCCGGTGCTCACATCAGCACCGGCAGCAACTGGATCGAGCTGGACATGAAG
GGCAACCGGCCGAAGGCGGTCAACGTGCGTACCGCGCCGTACCCGGCGTTCCCCACCGACATGCAGGCCCAG
TTCATCTCCATGAACGCGGTTGCCGAAGGCACCGGCGCGGTCATCGAGACGGTCTTCGAGAACCGCTTCATG
CATGTTTACGAAATGAACCGCATGGGTGCGCAGATCCTCGTCGAAGGCAACACCGCCATCGTCACCGGCGTA
CCCAAGCTCAAGGGCGCTCCGGTCATGGCGACCGACCTGCGCGCATCCGCGAGCCTGGTGATCGCCGGCCTG
GTGGCCGAGGGTGACACCCTGATCGATCGCATCTACCACATCGACCGTGGCTACGAGTGCATCGAAGAGAAA
CTCCAGCTGCTCGGCGCCAAGATCCGCCGCGTACCGGGCTAG
Translation:
MDKLIITGGNRLDGEIRISGAKNSALPILAATLLADTPVTVCNLPHLHDITTMIELFGRMGVQPIIDEKLNV
EVDASSIKTLVAPYELVKTMRASILVLGPMLARFGEAEVALPGGCAIGSRPVDLHIRGLEAMGAQIEVEGGY
IKAKAPAGGLRGGHFFFDTVSVTGTENLMMAAALANGRTVLQNAAREPEVVDLANCLNAMGANVQGAGSDTI
VIEGVKRLGGARYDVLPDRIETGTYLVAAAATGGRVKLKDTDPTILEAVLQKLEEAGAHISTGSNWIELDMK
GNRPKAVNVRTAPYPAFPTDMQAQFISMNAVAEGTGAVIETVFENRFMHVYEMNRMGAQILVEGNTAIVTGV
PKLKGAPVMATDLRASASLVIAGLVAEGDTLIDRIYHIDRGYECIEEKLQLLGAKIRRVPG*
AnnotationID:1380GeneID:1371
AnnotatorUID: diggins
Modification Date/Time: 2005-05-03 06:06:51
Gene Name:murA
Confidence Code:2
GeneProduct: UDP-N-acetylglucosamine 1-carboxyvinyltransferase
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(7) Cell wall / LPS / capsule
Alternate Gene Product Name: enoylpyruvate transferase
Functional Category Confidence Code:5
COGs:COG0766,pfam00275
Secondary Functional Category(ies):
EC Number:2.5.1.7
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAN66589.1|AE016286_13 (AE016777) UDP-N-acetylglucosamine 1-carboxyvinyltransferase
           [Pseudomonas putida KT2440]
          Length = 421

 Score =  729 bits (1883), Expect = 0.0
 Identities = 367/421 (87%), Positives = 397/421 (94%)

>gb|AAO57890.1| (AE016872) UDP-N-acetylglucosamine 1-carboxyvinyltransferase
           [Pseudomonas syringae pv. tomato str. DC3000]
          Length = 421

 Score =  722 bits (1863), Expect = 0.0
 Identities = 364/421 (86%), Positives = 394/421 (93%)






 
Structural Features:
COG0766, MurA, UDP-N-acetylglucosamine enolpyruvyl transferase 
[Cell envelope biogenesis, outer membrane]
CD-Length = 421 residues,  99.8% aligned
Score =  641 bits (1654), Expect = 0.0
Identities = 270/421 (64%), Positives = 339/421 (80%), Gaps = 1/421 (0%)

pfam00275, EPSP_synthase, EPSP synthase 
(3-phosphoshikimate 1-carboxyvinyltransferase).
CD-Length = 416 residues, 100.0% aligned
Score =  359 bits (924), Expect = 5e-100
Identities = 146/419 (34%), Positives = 211/419 (50%), Gaps = 18/419 (4%)






   
Genomic Context:






   
Comment:


















    ReferenceID:2745




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:19890




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:31488




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:1371_cdd.html


    		




 
URL:

















    ReferenceID:32844




  
Author/Investigator(s):
        Zoeiby AE, Beaumont M, Dubuc E, Sanschagrin F, Voyer N, Levesque RC.





  
Title:
        Combinatorial enzymatic assay for the screening of a new class of bacterial cell wall inhibitors.




  
PubMed:
        12628682


MedLine:
        




    
Source:
Bioorg Med Chem. 2003 Apr 3;11(7):1583-92.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=12628682

















    ReferenceID:32845




  
Author/Investigator(s):
        El Zoeiby A, Sanschagrin F, Darveau A, Brisson JR, Levesque RC.





  
Title:
        Identification of novel inhibitors of Pseudomonas aeruginosa MurC enzyme derived from phage-displayed peptide libraries.




  
PubMed:
        12615853


MedLine:
        




    
Source:
J Antimicrob Chemother. 2003 Mar;51(3):531-43.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=12615853

















    ReferenceID:32846




  
Author/Investigator(s):
        El Zoeiby A, Sanschagrin F, Havugimana PC, Garnier A, Levesque RC.





  
Title:
        In vitro reconstruction of the biosynthetic pathway of peptidoglycan cytoplasmic precursor in Pseudomonas aeruginosa.




  
PubMed:
        11470366


MedLine:
        




    
Source:
FEMS Microbiol Lett. 2001 Jul 24;201(2):229-35.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=11470366









           
Homologs By Global Alignment 

           

       



     
Gene ID:1371
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
2495
                           PA4450_tr
                           translation of PA4450
                           421
                           99.99
                           99.99
                           0
                           2120.0
     

     
     
78965
                           gb|AAG07838.1|AE004859_7
                           (AE004859) UDP-N-acetylglucosamine 1-carboxyvinyltransferase [Pseudomonas aeruginosa PAO1]
                           421
                           99.99
                           99.99
                           0
                           2120.0
     

     
     
78966
                           gb|AAN66589.1|AE016286_13
                           (AE016777) UDP-N-acetylglucosamine 1-carboxyvinyltransferase [Pseudomonas putida KT2440]
                           421
                           87.17
                           94.29
                           0
                           1883.0
     

     
     
78967
                           gb|AAO57890.1|
                           (AE016872) UDP-N-acetylglucosamine 1-carboxyvinyltransferase [Pseudomonas syringae pv. tomato str. DC3000]
                           421
                           86.46
                           93.58
                           0
                           1863.0
     

     
     
78968
                           gb|AAD17963.1|
                           (AF106002) UDP-N-acetylglucosamine enolpyruvyl transferase homolog [Pseudomonas putida]
                           421
                           85.27
                           92.16
                           2
                           1807.0
     

     






      

          
GC ORFID: 44373How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA4450Glimmer Score: 

          
Start: 4412740Stop: 4411475

          
Length: 1266


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TAGTruncated Stop: 

          
Homolog: t_PA4450 translation of PA4450Homolog Bit Score 821.0

          
Other Homologs:  psyr_15may02_Scaffold4_revised_gene5525 (ORF: BLASTX 720.0), t_PA4450 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGGATAAACTGATTATTACCGGCGGTAACCGCCTCGATGGCGAAATCCGCATTTCCGGCGCGAAGAACTCG 
GCGCTGCCGATCCTGGCCGCGACCCTGCTGGCCGATACTCCGGTCACCGTCTGCAACCTGCCGCACCTGCAC 
GACATTACCACCATGATCGAACTGTTCGGCCGCATGGGCGTGCAGCCGATCATCGACGAGAAGCTCAACGTC 
GAAGTCGACGCCAGCAGCATCAAGACCCTGGTCGCGCCGTACGAACTGGTGAAGACCATGCGTGCCTCGATC 
CTGGTGCTGGGTCCGATGCTGGCGCGCTTCGGCGAGGCCGAAGTGGCCCTGCCGGGCGGTTGCGCGATCGGT 
TCGCGTCCGGTCGACCTGCATATCCGCGGTCTCGAGGCCATGGGCGCGCAGATCGAGGTCGAAGGCGGCTAC 
ATCAAGGCCAAGGCGCCCGCCGGCGGCCTGCGTGGCGGTCACTTCTTCTTCGATACCGTCAGCGTGACCGGC 
ACCGAGAACCTGATGATGGCCGCCGCGCTGGCCAACGGCCGCACCGTGCTGCAGAACGCCGCTCGCGAGCCG 
GAGGTGGTCGACCTGGCCAACTGCCTGAACGCCATGGGCGCCAACGTCCAGGGCGCTGGCTCCGATACCATC 
GTCATCGAAGGCGTGAAGCGCCTCGGCGGCGCTCGCTACGACGTACTGCCCGACCGTATCGAGACCGGCACC 
TACCTGGTGGCCGCGGCCGCGACCGGCGGCCGGGTGAAGTTGAAGGATACCGATCCGACCATCCTCGAGGCG 
GTCCTGCAGAAGCTGGAAGAGGCCGGTGCTCACATCAGCACCGGCAGCAACTGGATCGAGCTGGACATGAAG 
GGCAACCGGCCGAAGGCGGTCAACGTGCGTACCGCGCCGTACCCGGCGTTCCCCACCGACATGCAGGCCCAG 
TTCATCTCCATGAACGCGGTTGCCGAAGGCACCGGCGCGGTCATCGAGACGGTCTTCGAGAACCGCTTCATG 
CATGTTTACGAAATGAACCGCATGGGTGCGCAGATCCTCGTCGAAGGCAACACCGCCATCGTCACCGGCGTA 
CCCAAGCTCAAGGGCGCTCCGGTCATGGCGACCGACCTGCGCGCATCCGCGAGCCTGGTGATCGCCGGCCTG 
GTGGCCGAGGGTGACACCCTGATCGATCGCATCTACCACATCGACCGTGGCTACGAGTGCATCGAAGAGAAA 
CTCCAGCTGCTCGGCGCCAAGATCCGCCGCGTACCGGGCTAG