ViewGene
Locus Name:
GeneID:
Location:

GeneID:1367
TrivialName: PA14_25690
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_25690
PAO1 Ortholog Locus:PA2965
Sequence Length:1245
Protein Length:414
Start: 2244526
Stop: 2245770
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG06353.1|AE004722_9 (AE004722) beta-ketoacyl-acyl carrier protein synthase II [Pseudomonas aeruginosa PAO1]
Identities = 414/414 (100%), Positives = 414/414 (100%)
Sequence:
GTGTCGCGTAGACGCGTCGTCATTACTGGCATGGGCATGCTGTCGCCGCTGGGCCTGGATGTCCCGAGCAGT
TGGGAAGGTATTCTCGCCGGTCGCAGCGGGATCGCCCCGATCGAGCATATGGACCTGTCCGCCTATTCCACC
CGCTTCGGTGGTTCGGTCAAGGGTTTCAATGTCGAGGAATACCTGTCCGCCAAGGAAGCCCGCAAGCTCGAC
CTGTTCATCCAGTACGGTCTCGCCGCCAGCTTCCAGGCCGTTCGCGATTCCGGCCTGGAGGTCACCGACGCC
AATCGTGAGCGTATCGGCGTATCCATGGGTTCCGGTATCGGCGGCCTGACCAATATCGAGAACAACTGCCGA
TCCCTGTTCGAGCAGGGGCCGCGGCGCATCTCGCCGTTCTTCGTGCCCGGTTCGATCATCAACATGGTTTCC
GGCTTCCTGTCGATCCACCTGGGTCTGCAGGGGCCCAACTACGCCCTCACCACCGCCTGCACCACCGGTACC
CACAGCATCGGCATGGCTGCTCGCAACATCGCCTATGGCGAGGCCGACGTGATGGTCGCCGGCGGTTCCGAA
ATGGCTGCCTGCGGTCTCGGCCTGGGCGGTTTCGGCGCGGCTCGCGCGCTCTCCACTCGCAACGACGAGCCG
ACCCGTGCCAGCCGTCCGTGGGACCGGGATCGCGACGGCTTCGTCCTGTCCGACGGTTCCGGCGCCCTGGTG
CTCGAAGAACTGGAGCACGCCCGTGCCCGCGGCGCGCGGATCTATGCCGAGCTGGTTGGCTTCGGCATGAGC
GGCGACGCCTTCCACATGACCGCGCCGCCGGAAGATGGCGCCGGTGCCGCCCGCTGCATGAAGAACGCGCTG
CGCGATGCCGGCCTGGATCCTCGTCAGGTCGATTACATCAACGCCCATGGCACCTCCACCCCGGCCGGCGAC
ATCGCCGAGATCGCCGCGGTGAAGAGCGTGTTCGGCGAGCATGCCCATGCGCTGTCGATGAGTTCGACCAAG
TCGATGACCGGCCACCTGTTGGGTGCCGCCGGTGCGGTGGAGGCGATCTTCAGCGTGCTGGCCCTGCGCGAC
CAGGTCGCCCCGCCGACCATCAACCTGGACAACCCGGACGAAGGTTGCGACCTCGACCTGGTGGCCCACGAA
GCCAAGCCGCGCAAGATCGACGTGGCCCTGTCGAACTCGTTCGGTTTCGGCGGGACCAACGGCACCCTGGTG
TTCCGCAGGTTCGCCGACTGA
Translation:
MSRRRVVITGMGMLSPLGLDVPSSWEGILAGRSGIAPIEHMDLSAYSTRFGGSVKGFNVEEYLSAKEARKLD
LFIQYGLAASFQAVRDSGLEVTDANRERIGVSMGSGIGGLTNIENNCRSLFEQGPRRISPFFVPGSIINMVS
GFLSIHLGLQGPNYALTTACTTGTHSIGMAARNIAYGEADVMVAGGSEMAACGLGLGGFGAARALSTRNDEP
TRASRPWDRDRDGFVLSDGSGALVLEELEHARARGARIYAELVGFGMSGDAFHMTAPPEDGAGAARCMKNAL
RDAGLDPRQVDYINAHGTSTPAGDIAEIAAVKSVFGEHAHALSMSSTKSMTGHLLGAAGAVEAIFSVLALRD
QVAPPTINLDNPDEGCDLDLVAHEAKPRKIDVALSNSFGFGGTNGTLVFRRFAD*
AnnotationID:1376GeneID:1367
AnnotatorUID: diggins
Modification Date/Time: 2005-04-11 06:06:12
Gene Name:fabF1
Confidence Code:1
GeneProduct:beta-ketoacyl-acyl carrier protein synthase II
Cell Localization:()
Synonyms:fabF
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(13) Fatty acid and phospholipid metabolism
Alternate Gene Product Name:3-oxoacyl-acyl carrier protein synthase II
Functional Category Confidence Code:5
COGs:cd00834,COG0304
Secondary Functional Category(ies):Secondary metabolites biosynthesis, transport, and catabolism
EC Number:2.3.1.41
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAN67534.1|AE016382_2 (AE016781) 3-oxoacyl-(acyl-carrier-protein) synthase II
           [Pseudomonas putida KT2440]
          Length = 414

 Score =  714 bits (1844), Expect = 0.0
 Identities = 347/413 (84%), Positives = 382/413 (92%)

>gb|AAO57298.1| (AE016869) 3-oxoacyl-(acyl-carrier-protein) synthase II
           [Pseudomonas syringae pv. tomato str. DC3000]
          Length = 414

 Score =  699 bits (1805), Expect = 0.0
 Identities = 345/414 (83%), Positives = 382/414 (92%)

>gb|AAN55799.1|AE015716_9 (AE015716) 3-oxoacyl-(acyl-carrier-protein) synthase II [Shewanella
           oneidensis MR-1]
          Length = 412

 Score =  581 bits (1498), Expect = e-165
 Identities = 277/411 (67%), Positives = 344/411 (83%)






 
Structural Features:
cd00834, KAS_I_II, Beta-ketoacyl-acyl carrier protein (ACP) synthase (KAS), 
type I and II.
CD-Length = 406 residues, 100.0% aligned
Score =  561 bits (1447), Expect = 6e-161

COG0304, FabB, 3-oxoacyl-(acyl-carrier-protein) synthase 
[Lipid metabolism / Secondary metabolites biosynthesis, transport, and catabolism]
CD-Length = 412 residues,  99.8% aligned
Score =  503 bits (1296), Expect = 2e-143
Identities = 234/413 (56%), Positives = 301/413 (72%), Gaps = 4/413 (0%)








   
Genomic Context:






   
Comment:


















    ReferenceID:14339




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:19882




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:36084




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:1367_cdd.html


    		




 
URL:

















    ReferenceID:36437




  
Author/Investigator(s):
        Shen Z, Byers DM.





  
Title:
        Isolation of Vibrio harveyi acyl carrier protein and the fabG, acpP, and fabF genes involved in fatty acid biosynthesis.




  
PubMed:
        8550484


MedLine:
        




    
Source:
J Bacteriol. 1996 Jan;178(2):571-3.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=8550484

















    ReferenceID:36440




  
Author/Investigator(s):
        Kutchma AJ, Hoang TT, Schweizer HP.





  
Title:
        Characterization of a Pseudomonas aeruginosa fatty acid biosynthetic gene cluster: purification of acyl carrier protein (ACP) and malonyl-coenzyme A:ACP transacylase (FabD).




  
PubMed:
        10464226


MedLine:
        




    
Source:
J Bacteriol. 1999 Sep;181(17):5498-504.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=10464226









           
Homologs By Global Alignment 

           

       



     
Gene ID:1367
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
2487
                           PA2965_tr
                           translation of PA2965
                           414
                           99.99
                           99.99
                           0
                           2122.0
     

     
     
78507
                           gb|AAB94396.1|
                           (U91631) 3-oxoacyl-acyl carrier protein synthase II [Pseudomonas aeruginosa]
                           414
                           99.75
                           99.99
                           0
                           2119.0
     

     
     
78506
                           gb|AAG06353.1|AE004722_9
                           (AE004722) beta-ketoacyl-acyl carrier protein synthase II [Pseudomonas aeruginosa PAO1]
                           414
                           99.75
                           99.99
                           0
                           2119.0
     

     
     
78508
                           gb|AAN67534.1|AE016382_2
                           (AE016781) 3-oxoacyl-(acyl-carrier-protein) synthase II [Pseudomonas putida KT2440]
                           414
                           83.57
                           92.27
                           0
                           1839.0
     

     
     
78509
                           gb|AAO57298.1|
                           (AE016869) 3-oxoacyl-(acyl-carrier-protein) synthase II [Pseudomonas syringae pv. tomato str. DC3000]
                           414
                           83.09
                           92.27
                           0
                           1801.0
     

     






      

          
GC ORFID: 44369How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA2965Glimmer Score: 

          
Start: 2709399Stop: 2708155

          
Length: 1245


          
Start Codon: GTGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA2965 translation of PA2965Homolog Bit Score 822.0

          
Other Homologs:  psyr_15may02_Scaffold16_revised_gene2610 (ORF: BLASTX 703.0), t_PA1373 (ORF: BLASTX 383.0), psyr_15may02_Scaffold1_revised_gene427 (ORF: BLASTX 279.0), t_PA1609 (ORF: BLASTX 278.0), t_PA2965 (ORF: GLIMMER)

          
GC ORF Sequence

           
GTGTCGCGTAGACGCGTCGTCATTACTGGCATGGGCATGCTGTCGCCGCTGGGCCTGGATGTCCCGAGCAGT 
TGGGAAGGTATTCTCGCCGGTCGCAGCGGGATCGCCCCGATCGAGCATATGGACCTGTCCGCCTATTCCACC 
CGCTTCGGTGGTTCGGTCAAGGGTTTCAATGTCGAGGAATACCTGTCCGCCAAGGAAGCCCGCAAGCTCGAC 
CTGTTCATCCAGTACGGTCTCGCCGCCAGCTTCCAGGCCGTTCGCGATTCCGGCCTGGAGGTCACCGACGCC 
AATCGTGAGCGTATCGGCGTATCCATGGGTTCCGGTATCGGCGGCCTGACCAATATCGAGAACAACTGCCGA 
TCCCTGTTCGAGCAGGGGCCGCGGCGCATCTCGCCGTTCTTCGTGCCCGGTTCGATCATCAACATGGTTTCC 
GGCTTCCTGTCGATCCACCTGGGTCTGCAGGGGCCCAACTACGCCCTCACCACCGCCTGCACCACCGGTACC 
CACAGCATCGGCATGGCTGCTCGCAACATCGCCTATGGCGAGGCCGACGTGATGGTCGCCGGCGGTTCCGAA 
ATGGCTGCCTGCGGTCTCGGCCTGGGCGGTTTCGGCGCGGCTCGCGCGCTCTCCACTCGCAACGACGAGCCG 
ACCCGTGCCAGCCGTCCGTGGGACCGGGATCGCGACGGCTTCGTCCTGTCCGACGGTTCCGGCGCCCTGGTG 
CTCGAAGAACTGGAGCACGCCCGTGCCCGCGGCGCGCGGATCTATGCCGAGCTGGTTGGCTTCGGCATGAGC 
GGCGACGCCTTCCACATGACCGCGCCGCCGGAAGATGGCGCCGGTGCCGCCCGCTGCATGAAGAACGCGCTG 
CGCGATGCCGGCCTGGATCCTCGTCAGGTCGATTACATCAACGCCCATGGCACCTCCACCCCGGCCGGCGAC 
ATCGCCGAGATCGCCGCGGTGAAGAGCGTGTTCGGCGAGCATGCCCATGCGCTGTCGATGAGTTCGACCAAG 
TCGATGACCGGCCACCTGTTGGGTGCCGCCGGTGCGGTGGAGGCGATCTTCAGCGTGCTGGCCCTGCGCGAC 
CAGGTCGCCCCGCCGACCATCAACCTGGACAACCCGGACGAAGGTTGCGACCTCGACCTGGTGGCCCACGAA 
GCCAAGCCGCGCAAGATCGACGTGGCCCTGTCGAACTCGTTCGGTTTCGGCGGGACCAACGGCACCCTGGTG 
TTCCGCAGGTTCGCCGACTGA