ViewGene
Locus Name:
GeneID:
Location:

GeneID:1364
TrivialName: PA14_35500
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_35500
PAO1 Ortholog Locus:PA2249
Sequence Length:1287
Protein Length:428
Start: 3157346
Stop: 3156060
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG05637.1|AE004650_8 (AE004650) branched-chain alpha-keto acid dehydrogenase (lipoamide component) [Pseudomonas aeruginosa PAO1]
Identities = 422/428 (98%), Positives = 424/428 (99%)
Sequence:
ATGGGTACCCATGTGATCAAGATGCCGGACATCGGGGAAGGCATCGCCGAGGTCGAACTGGTGGAGTGGCAT
GTCCAGGTCGGCGACTCGGTCAATGAAGACCAGGTCCTCGCCGAGGTGATGACCGACAAGGCCACGGTGGAG
ATTCCCTCGCCGGTGGCCGGACGCATCCTCGCCCTCGGCGGCCAGCCGGGCCAGGTGATGGCGGTGGGCGGC
GAACTGATCCGCCTGGAGGTGGAAGGCGCCGGCAACCTCGCCGAGAGTCCGGCCGCGGCGACGCCGGCCGCG
CCCGTCGCAGCCGCCCCGGAGGAACCCAAGGAGACCCCGGTCGCGGCGCCGAAAGCCGCCGCCGAAGCGCCG
CGCGCCTTGCGCGACAGCGAGGCGCCACGGCAGCGGCGCCAGCCCGGCGAACGCCCGCTGGCCTCCCCCGCG
GTGCGCCAGCGCGCCCGCGACCTGGGCATCGAGTTGCAGTTCGTGCAGGGCAGCGGTCCCGCCGGACGCGTC
CTCCACGAGGACCTCGATGCCTACCTGACCCAGGACGGCAGCGTCACGCGCAGCGGCGGCGCCGCGCAAGGG
TATGCCGAGCGACACGACGAACAGGCGGTGCCGGTGATCGGCCTGCGTCGCAAGATCGCCCAGAAGATGCAG
GACGCCAAGCGACGCATCCCGCATTTCAGCTATGTCGAGGAAGTCGACGTCACCGACCTGGAAGCCCTGCGC
GCCCATCTCAACCAGAAATGGGGTGGCCAGCGCGGCAAGCTGACCCTGCTGCCGTTCCTGGTCCGCGCCATG
GTCGTGGCGCTGCGCGACTTCCCGCAGTTGAACGCGCGCTACGACGACGAGGCCGAGGTGGTCACCCGCTAC
GGCGCGGTGCACGTCGGCATCGCCACCCAGAGCGACAACGGCCTGATGGTGCCGGTGCTGCGCCACGCCGAA
TCGCGCGACCTCTGGGGCAACGCCAGCGAAGTGGCGCGCCTGGCCGAAGCCGCGCGCAGCGGCAAGGCGCAA
CGCCAGGAGCTGTCCGGCTCGACCATCACCCTGAGCAGCCTCGGCGCGCTCGGCGGGATCGTCAGCACACCG
GTGATCAACCATCCTGAGGTGGCCATCGTCGGCGTCAACCGCATCGTCGAGCGACCGATGGTGGTCGGCGGC
AACATCGTCGTGCGCAAGATGATGAACCTCTCCTCCTCCTTCGACCACCGGGTGGTCGACGGGATGGACGCG
GCGGCCTTCATCCAGGCCGTGCGCGGCCTGCTCGAACATCCTGCCACCCTGTTCCTGGAGTAA
Translation:
MGTHVIKMPDIGEGIAEVELVEWHVQVGDSVNEDQVLAEVMTDKATVEIPSPVAGRILALGGQPGQVMAVGG
ELIRLEVEGAGNLAESPAAATPAAPVAAAPEEPKETPVAAPKAAAEAPRALRDSEAPRQRRQPGERPLASPA
VRQRARDLGIELQFVQGSGPAGRVLHEDLDAYLTQDGSVTRSGGAAQGYAERHDEQAVPVIGLRRKIAQKMQ
DAKRRIPHFSYVEEVDVTDLEALRAHLNQKWGGQRGKLTLLPFLVRAMVVALRDFPQLNARYDDEAEVVTRY
GAVHVGIATQSDNGLMVPVLRHAESRDLWGNASEVARLAEAARSGKAQRQELSGSTITLSSLGALGGIVSTP
VINHPEVAIVGVNRIVERPMVVGGNIVVRKMMNLSSSFDHRVVDGMDAAAFIQAVRGLLEHPATLFLE*
AnnotationID:1373GeneID:1364
AnnotatorUID: diggins
Modification Date/Time: 2005-04-29 06:06:32
Gene Name:bkdB
Confidence Code:2
GeneProduct:lipoamide acyltransferase component of branched-chain alpha-keto acid dehydrogenase complex E2
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(2) Amino acid biosynthesis and metabolism
Alternate Gene Product Name:2-oxoisovalerate dehydrogenase, lipoamide acyltransferase component
Functional Category Confidence Code:5
COGs:COG0508,pfam00198
Secondary Functional Category(ies):energy metabolism
EC Number:2.3.1.168
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAN69981.1|AE016636_4 (AE016790) 2-oxoisovalerate dehydrogenase, lipoamide
           acyltransferase component [Pseudomonas putida KT2440]
          Length = 423

 Score =  600 bits (1547), Expect = e-171
 Identities = 310/431 (71%), Positives = 358/431 (83%), Gaps = 11/431 (2%)

>dbj|BAB51121.1| (AP003004) dihydrolipoamide S-(2-methylpropanoyl)transferase
           [Mesorhizobium loti]
          Length = 438

 Score =  465 bits (1196), Expect = e-130
 Identities = 246/442 (55%), Positives = 321/442 (72%), Gaps = 18/442 (4%)

>dbj|BAC51598.1| (AP005958) lipoamide acyltransferase component of branched-chain
           alpha-keto acid dehydrogenase complex E2 [Bradyrhizobium
           japonicum USDA 110]
          Length = 427

 Score =  429 bits (1102), Expect = e-119
 Identities = 232/433 (53%), Positives = 305/433 (70%), Gaps = 12/433 (2%)






 
Structural Features:
COG0508, AceF, Pyruvate/2-oxoglutarate dehydrogenase complex, 
dihydrolipoamide acyltransferase (E2) component, 
and related enzymes 
[Energy production and conversion]
CD-Length = 404 residues,  99.8% aligned
Score =  334 bits (857), Expect = 1e-92
Identities = 179/428 (41%), Positives = 257/428 (60%), Gaps = 27/428 (6%)

pfam00198, 2-oxoacid_dh, 2-oxoacid dehydrogenases acyltransferase 
(catalytic domain)
CD-Length = 232 residues,  99.1% aligned
Score =  280 bits (719), Expect = 2e-76
Identities = 102/230 (44%), Positives = 153/230 (66%)






   
Genomic Context:






   
Comment:


















    ReferenceID:2731




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:19876




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:32526




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:1364_cdd.html


    		




 
URL:

















    ReferenceID:34443




  
Author/Investigator(s):
        Burns G, Brown T, Hatter K, Sokatch JR.





  
Title:
        Comparison of the amino acid sequences of the transacylase components of branched chain oxoacid dehydrogenase of Pseudomonas putida, and the pyruvate and 2-oxoglutarate dehydrogenases of Escherichia coli.




  
PubMed:
        3046941


MedLine:
        




    
Source:
Eur J Biochem. 1988 Sep 1;176(1):165-9.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=3046941









           
Homologs By Global Alignment 

           

       



     
Gene ID:1364
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
2484
                           PA2249_tr
                           translation of PA2249
                           428
                           98.59
                           99.06
                           0
                           2127.0
     

     
     
78483
                           gb|AAG05637.1|AE004650_8
                           (AE004650) branched-chain alpha-keto acid dehydrogenase (lipoamide component) [Pseudomonas aeruginosa PAO1]
                           428
                           98.59
                           99.06
                           0
                           2127.0
     

     
     
78484
                           gb|AAA65617.1|
                           (M57613) transacylase E2 [Pseudomonas putida]
                           431
                           72.38
                           83.29
                           11
                           1563.0
     

     
     
78485
                           gb|AAN69981.1|AE016636_4
                           (AE016790) 2-oxoisovalerate dehydrogenase, lipoamide acyltransferase component [Pseudomonas putida KT2440]
                           431
                           71.92
                           83.06
                           11
                           1557.0
     

     
     
78486
                           dbj|BAB51121.1|
                           (AP003004) dihydrolipoamide S-(2-methylpropanoyl)transferase [Mesorhizobium loti]
                           443
                           56.20
                           73.36
                           20
                           1213.0
     

     






      

          
GC ORFID: 44366How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA2249Glimmer Score: 

          
Start: 1796570Stop: 1797856

          
Length: 1287


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TAATruncated Stop: 

          
Homolog: t_PA2249 translation of PA2249Homolog Bit Score 823.0

          
Other Homologs:  t_PA2249 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGGGTACCCATGTGATCAAGATGCCGGACATCGGGGAAGGCATCGCCGAGGTCGAACTGGTGGAGTGGCAT 
GTCCAGGTCGGCGACTCGGTCAATGAAGACCAGGTCCTCGCCGAGGTGATGACCGACAAGGCCACGGTGGAG 
ATTCCCTCGCCGGTGGCCGGACGCATCCTCGCCCTCGGCGGCCAGCCGGGCCAGGTGATGGCGGTGGGCGGC 
GAACTGATCCGCCTGGAGGTGGAAGGCGCCGGCAACCTCGCCGAGAGTCCGGCCGCGGCGACGCCGGCCGCG 
CCCGTCGCAGCCGCCCCGGAGGAACCCAAGGAGACCCCGGTCGCGGCGCCGAAAGCCGCCGCCGAAGCGCCG 
CGCGCCTTGCGCGACAGCGAGGCGCCACGGCAGCGGCGCCAGCCCGGCGAACGCCCGCTGGCCTCCCCCGCG 
GTGCGCCAGCGCGCCCGCGACCTGGGCATCGAGTTGCAGTTCGTGCAGGGCAGCGGTCCCGCCGGACGCGTC 
CTCCACGAGGACCTCGATGCCTACCTGACCCAGGACGGCAGCGTCACGCGCAGCGGCGGCGCCGCGCAAGGG 
TATGCCGAGCGACACGACGAACAGGCGGTGCCGGTGATCGGCCTGCGTCGCAAGATCGCCCAGAAGATGCAG 
GACGCCAAGCGACGCATCCCGCATTTCAGCTATGTCGAGGAAGTCGACGTCACCGACCTGGAAGCCCTGCGC 
GCCCATCTCAACCAGAAATGGGGTGGCCAGCGCGGCAAGCTGACCCTGCTGCCGTTCCTGGTCCGCGCCATG 
GTCGTGGCGCTGCGCGACTTCCCGCAGTTGAACGCGCGCTACGACGACGAGGCCGAGGTGGTCACCCGCTAC 
GGCGCGGTGCACGTCGGCATCGCCACCCAGAGCGACAACGGCCTGATGGTGCCGGTGCTGCGCCACGCCGAA 
TCGCGCGACCTCTGGGGCAACGCCAGCGAAGTGGCGCGCCTGGCCGAAGCCGCGCGCAGCGGCAAGGCGCAA 
CGCCAGGAGCTGTCCGGCTCGACCATCACCCTGAGCAGCCTCGGCGCGCTCGGCGGGATCGTCAGCACACCG 
GTGATCAACCATCCTGAGGTGGCCATCGTCGGCGTCAACCGCATCGTCGAGCGACCGATGGTGGTCGGCGGC 
AACATCGTCGTGCGCAAGATGATGAACCTCTCCTCCTCCTTCGACCACCGGGTGGTCGACGGGATGGACGCG 
GCGGCCTTCATCCAGGCCGTGCGCGGCCTGCTCGAACATCCTGCCACCCTGTTCCTGGAGTAA