ViewGene
Locus Name:
GeneID:
Location:

GeneID:136
TrivialName: PA14_16690
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_16690
PAO1 Ortholog Locus:PA3687
Sequence Length:2637
Protein Length:878
Start: 1427626
Stop: 1430262
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG07075.1|AE004788_5 (AE004788) phosphoenolpyruvate carboxylase [Pseudomonas aeruginosa PAO1]
Identities = 875/878 (99%), Positives = 877/878 (99%)
Sequence:
ATGCCGGAAATCGATGCGCGCCTGCGCGAGGACGTCCACCAACTGGGCGAATTGCTCGGCGACACCATCCGC
GAGCAGTACGGCCCGCGGTTCCTCGACAAGATCGAGCTGATCCGCAAGGGCGCCAAGGCTGCCCGCCGCGGT
TCCGCGGAGGGCGCCCAGCAGCTCACCGCTACCCTCGACGGGCTCGAGGAAGACGAACTGCTGCCGGTGGCG
CGGGCCTTCAACCAGTTCCTCAACCTGGCCAATATCGCCGAGCAATACCACCGTATCCGCCGGCGCCGGCCG
AACGAGCCGGAACCCTTCGAGAACCTGGTGCTGGAAGAGCTGCTGGGGCGTCTCAAGGACGCCGGGCATGCC
CCCGGGCAACTGGCTCGCCAGCTCGCCGGGCTGGAGATCGAACTGGTGCTGACCGCCCATCCCACCGAGGTG
GCGCGGCGCACGCTGATCCAGAAATACGATGCGATCACCGCGCAGCTGGCGGCCAAGGACCATGCCGACCTG
CTGCCGGAGGAGCGCAGTCGCATCCAGCAGCGCCTGCAGCGGCTGGTGGCCGAGGCCTGGCATACCGACGAG
ATCCGCAAGGTCCGGCCGACCCCGGTGGACGAGGCGAAATGGGGCTTCGCGGTGATCGAGCATTCTCTCTGG
CAGGCCTTGCCGAACGTCCTTCGGCACGTCGACGAGGTACTCCTGCGCAGCACCGGCGAACGCCTGCCGCTG
ACCGCCGCGCCGCTGCGCTTCGCGTCCTGGATGGGGGGCGACCGCGACGGCAATCCCAACGTTACCGCCAGC
GTGACCCGCGAGGTCCTGCTGCTGGCGCGCTGGATGGCCGCCGACCTGTACCTGCGCGATATCGACCGACTG
GCCGCCGAGCTGTCCATGCAGCAGGCCTCGCCGCAGCTGCTGGCGCGGGTCGGCGATAGCGCCGAGCCCTAT
CGCGCCCTGCTCAAGCAATTGCGCGAGCGCCTGCGCGTGACCCGCAACTGGACCCACCAGGCCCTGGCCGGC
GAGGTGCCGGCCGCGGAGGGCGTCCTGGAGCACAACCGCGACCTGGTCGAGCCGCTGCAACTGTGCCACGAG
TCGCTGCACGCCTGCGGCATGGGCGTGATCGCCGATGGCGCGTTGCTCGACTGCCTGCGCCGCGCCGCCACC
TTCGGCCTGTTCCTGGTACGCCTGGACGTGCGCCAGGACTCGGCCCGCCATGCCGCCGCGCTGAGCGAGATC
ACCGAGTACCTGGAGCTGGGCAGCTACGACGAGTGGGACGAGAAGACCCGCCTGGAATTCCTTCTCGAGGAA
CTGAACAGCCGCCGCCCCCTGCTGCCGGCGCACTACCAGCCTTCGGCGGATACCGCCGAGGTGCTCGCCACC
TGTCGGGCGATCGCCGCCGCGCCACCGGCGTCGCTGGGTTCCTACGTGATCTCGATGGCCGGCCAGCCGTCC
GACGTGCTGGCAGTGCAACTACTGCTCAAGGAAAGCGGCGTCGACTGGCCGATGCGCGTGGTGCCGCTGTTC
GAGACCCTCGACGACCTGGACAACGCCGGCCCCTGCATGGAGCGCCTGCTGACGCTGCCGGGCTACCGTTCG
CGGCTCTCCGGGGTGCAGGAAGTGATGATCGGCTACTCCGATTCGGCCAGGGACGCCGGTACTCTCACGGCG
GCCTGGGCGCAGTACCGGGCGCAGGAGAAACTGGTGGAGATCTGCCGCCAGCAAGAGGTCGAGCTGCTGCTC
TTCCATGGCCGCGGCGGCACCGTCGGCCGCGGCGGCGGGCCGGCCCACGCGGCGATCCTGTCGCAGCCACCG
GGTTCGGTGGCCGGGCGCTTCCGGGTCACCGAACAGGGCGAGATGATCCGCTTCAAGTTCGGCTTGCCGGAC
ATCGCCGAACAGAACCTCAATCTCTACCTCGCCGCAGTGCTGGAGGCGACCCTGATGCCGCCGCCGGCTCCC
GAGCCGGCCTGGCGCGCGCAGATGGACCGCCTGGCGAAGGACGCGCTGCTGGCCTACCGCAGGGTGGTGCGC
GACGATCCGCAGTTCGTCGAGTATTTCCGCCTGGCCACGCCCGAGCAGGAACTGGGCCGTCTGCCGCTGGGC
AGCCGCCCGGCCAAGCGCCGCGAAGGCGGGGTGGAGAGCCTGCGGGCGATTCCCTGGATCTTCGCCTGGACC
CAGACGCGCCTGATGCTGCCGGCATGGCTTGGCTGGGAGACCGCCTTGCTGAACGCCATCGAGCGTGGCGAG
GGCGCGCTGCTCGGGCAGATGCGCGAGCAATGGCCGTTCTTCACCACCCGCATCGACATGCTGGAAATGGTC
CTGGCCAAGGCCGACGCGGATATCGCGCGGCTCTACGACGAACGTCTAGTGCCGCTCGAGCTGCGTCCGCTG
GGGCGTCGTTTACGCGACCTATTGTCGCAGGCGGTGCGCGTGGTGCTGGGACTGACCGGGCAGTCGCTGCTG
CTCGCCCACGCCAGCGAGACCCGCGAGTCGATCAGCGTGCGCAACAGCTATCTAGACCCGCTGCACCTGCTC
CAGGCTGAGCTTCTGGCCCGTTCGCGGCGCTGCCAGGGCGACGCCTGCGGCGGCCTGGAGCAGGCGCTGCTG
GTGACGGTCGCGGGGGTCGCCGCCGGACTGCGCAACACCGGATGA
Translation:
MPEIDARLREDVHQLGELLGDTIREQYGPRFLDKIELIRKGAKAARRGSAEGAQQLTATLDGLEEDELLPVA
RAFNQFLNLANIAEQYHRIRRRRPNEPEPFENLVLEELLGRLKDAGHAPGQLARQLAGLEIELVLTAHPTEV
ARRTLIQKYDAITAQLAAKDHADLLPEERSRIQQRLQRLVAEAWHTDEIRKVRPTPVDEAKWGFAVIEHSLW
QALPNVLRHVDEVLLRSTGERLPLTAAPLRFASWMGGDRDGNPNVTASVTREVLLLARWMAADLYLRDIDRL
AAELSMQQASPQLLARVGDSAEPYRALLKQLRERLRVTRNWTHQALAGEVPAAEGVLEHNRDLVEPLQLCHE
SLHACGMGVIADGALLDCLRRAATFGLFLVRLDVRQDSARHAAALSEITEYLELGSYDEWDEKTRLEFLLEE
LNSRRPLLPAHYQPSADTAEVLATCRAIAAAPPASLGSYVISMAGQPSDVLAVQLLLKESGVDWPMRVVPLF
ETLDDLDNAGPCMERLLTLPGYRSRLSGVQEVMIGYSDSARDAGTLTAAWAQYRAQEKLVEICRQQEVELLL
FHGRGGTVGRGGGPAHAAILSQPPGSVAGRFRVTEQGEMIRFKFGLPDIAEQNLNLYLAAVLEATLMPPPAP
EPAWRAQMDRLAKDALLAYRRVVRDDPQFVEYFRLATPEQELGRLPLGSRPAKRREGGVESLRAIPWIFAWT
QTRLMLPAWLGWETALLNAIERGEGALLGQMREQWPFFTTRIDMLEMVLAKADADIARLYDERLVPLELRPL
GRRLRDLLSQAVRVVLGLTGQSLLLAHASETRESISVRNSYLDPLHLLQAELLARSRRCQGDACGGLEQALL
VTVAGVAAGLRNTG*
AnnotationID:146GeneID:136
AnnotatorUID: diggins
Modification Date/Time: 2005-04-21 11:11:51
Gene Name:ppc
Confidence Code:2
GeneProduct:phosphoenolpyruvate carboxylase
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(12) Energy metabolism
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:pfam00311,COG2352
Secondary Functional Category(ies):
EC Number:4.1.1.31
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAO55028.1| (AE016861) phosphoenolpyruvate carboxylase [Pseudomonas syringae
           pv. tomato str. DC3000]
          Length = 878

 Score = 1363 bits (3529), Expect = 0.0
 Identities = 691/878 (78%), Positives = 761/878 (86%)

>gb|AAP18739.1| (AE016990) phosphoenolpyruvate carboxylase [Shigella flexneri 2a
           str. 2457T]
          Length = 883

 Score =  959 bits (2480), Expect = 0.0
 Identities = 495/886 (55%), Positives = 637/886 (71%), Gaps = 11/886 (1%)

>gb|AAC76938.1| (AE000469) phosphoenolpyruvate carboxylase [Escherichia coli K12]
          Length = 883

 Score =  958 bits (2476), Expect = 0.0
 Identities = 494/886 (55%), Positives = 636/886 (71%), Gaps = 11/886 (1%)







 
Structural Features:
pfam00311, PEPcase, Phosphoenolpyruvate carboxylase.
CD-Length = 962 residues,  96.5% aligned
Score =  691 bits (1785), Expect = 0.0
Identities = 380/928 (40%), Positives = 528/928 (56%), Gaps = 70/928 (7%)

COG2352, Ppc, Phosphoenolpyruvate carboxylase 
[Energy production and conversion]
CD-Length = 910 residues,  99.7% aligned
Score = 1088 bits (2816), Expect = 0.0
Identities = 485/911 (53%), Positives = 616/911 (67%), Gaps = 39/911 (4%)






   
Genomic Context:






   
Comment:


















    ReferenceID:275




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:17417




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:31859




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:136_cdd.html


    		




 
URL:

















    ReferenceID:34245




  
Author/Investigator(s):
        Takai K, Sako Y, Uchida A.





  
Title:
        ppc, the gene for phosphoenolpyruvate carboxylase from an extremely thermophilic bacterium, Rhodothermus obamensis: cloning, sequencing and overexpression in Escherichia coli.




  
PubMed:
        9611816


MedLine:
        




    
Source:
Microbiology. 1998 May;144 ( Pt 5):1423-34.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=9611816

















    ReferenceID:34246




  
Author/Investigator(s):
        Nakamura T, Yoshioka I, Takahashi M, Toh H, Izui K.





  
Title:
        Cloning and sequence analysis of the gene for phosphoenolpyruvate carboxylase from an extreme thermophile, Thermus sp.




  
PubMed:
        8543565


MedLine:
        




    
Source:
J Biochem (Tokyo). 1995 Aug;118(2):319-24.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=8543565

















    ReferenceID:34247




  
Author/Investigator(s):
        Luinenburg I, Coleman JR.





  
Title:
        Identification, characterization and sequence analysis of the gene encoding phosphoenolpyruvate carboxylase in Anabaena sp. PCC 7120.




  
PubMed:
        1588304


MedLine:
        




    
Source:
J Gen Microbiol. 1992 Apr;138 ( Pt 4):685-91.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=1588304

















    ReferenceID:34248




  
Author/Investigator(s):
        Eikmanns BJ, Follettie MT, Griot MU, Sinskey AJ.





  
Title:
        The phosphoenolpyruvate carboxylase gene of Corynebacterium glutamicum: molecular cloning, nucleotide sequence, and expression.




  
PubMed:
        2779518


MedLine:
        




    
Source:
Mol Gen Genet. 1989 Aug;218(2):330-9.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=2779518

















    ReferenceID:34249




  
Author/Investigator(s):
        O'Regan M, Thierbach G, Bachmann B, Villeval D, Lepage P, Viret JF, Lemoine Y.





  
Title:
        Cloning and nucleotide sequence of the phosphoenolpyruvate carboxylase-coding gene of Corynebacterium glutamicum ATCC13032.




  
PubMed:
        2666264


MedLine:
        




    
Source:
Gene. 1989 Apr 30;77(2):237-51.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=2666264

















    ReferenceID:34250




  
Author/Investigator(s):
        Katagiri F, Kodaki T, Fujita N, Izui K, Katsuki H.





  
Title:
        Nucleotide sequence of the phosphoenolpyruvate carboxylase gene of the cyanobacterium Anacystis nidulans.




  
PubMed:
        2998946


MedLine:
        




    
Source:
Gene. 1985;38(1-3):265-9.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=2998946

















    ReferenceID:34251




  
Author/Investigator(s):
        Fujita N, Miwa T, Ishijima S, Izui K, Katsuki H.





  
Title:
        The primary structure of phosphoenolpyruvate carboxylase of Escherichia coli. Nucleotide sequence of the ppc gene and deduced amino acid sequence.




  
PubMed:
        6086598


MedLine:
        




    
Source:
J Biochem (Tokyo). 1984 Apr;95(4):909-16.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=6086598









           
Homologs By Global Alignment 

           

       



     
Gene ID:136
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
249
                           PA3687_tr
                           translation of PA3687
                           878
                           99.65
                           99.88
                           0
                           4469.0
     

     
     
17421
                           gb|AAG07075.1|AE004788_5
                           (AE004788) phosphoenolpyruvate carboxylase [Pseudomonas aeruginosa PAO1]
                           878
                           99.65
                           99.88
                           0
                           4469.0
     

     
     
17422
                           gb|AAO55028.1|
                           (AE016861) phosphoenolpyruvate carboxylase [Pseudomonas syringae pv. tomato str. DC3000]
                           878
                           78.70
                           86.67
                           0
                           3529.0
     

     
     
17423
                           gb|AAN67126.1|AE016341_9
                           (AE016779) phosphoenolpyruvate carboxylase [Pseudomonas putida KT2440]
                           878
                           75.96
                           84.51
                           3
                           3394.0
     

     
     
17434
                           emb|CAD09510.1|
                           (AL627279) phosphoenolpyruvate carboxylase [Salmonella enterica subsp. enterica serovar Typhi]
                           890
                           56.17
                           71.46
                           19
                           2489.0
     

     






      

          
GC ORFID: 43138How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA3687Glimmer Score: 

          
Start: 3526311Stop: 3523675

          
Length: 2637


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA3687 translation of PA3687Homolog Bit Score 1726.0

          
Other Homologs:  psyr_15may02_Scaffold3_revised_gene4600 (ORF: BLASTX 1360.0), t_PA3687 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGCCGGAAATCGATGCGCGCCTGCGCGAGGACGTCCACCAACTGGGCGAATTGCTCGGCGACACCATCCGC 
GAGCAGTACGGCCCGCGGTTCCTCGACAAGATCGAGCTGATCCGCAAGGGCGCCAAGGCTGCCCGCCGCGGT 
TCCGCGGAGGGCGCCCAGCAGCTCACCGCTACCCTCGACGGGCTCGAGGAAGACGAACTGCTGCCGGTGGCG 
CGGGCCTTCAACCAGTTCCTCAACCTGGCCAATATCGCCGAGCAATACCACCGTATCCGCCGGCGCCGGCCG 
AACGAGCCGGAACCCTTCGAGAACCTGGTGCTGGAAGAGCTGCTGGGGCGTCTCAAGGACGCCGGGCATGCC 
CCCGGGCAACTGGCTCGCCAGCTCGCCGGGCTGGAGATCGAACTGGTGCTGACCGCCCATCCCACCGAGGTG 
GCGCGGCGCACGCTGATCCAGAAATACGATGCGATCACCGCGCAGCTGGCGGCCAAGGACCATGCCGACCTG 
CTGCCGGAGGAGCGCAGTCGCATCCAGCAGCGCCTGCAGCGGCTGGTGGCCGAGGCCTGGCATACCGACGAG 
ATCCGCAAGGTCCGGCCGACCCCGGTGGACGAGGCGAAATGGGGCTTCGCGGTGATCGAGCATTCTCTCTGG 
CAGGCCTTGCCGAACGTCCTTCGGCACGTCGACGAGGTACTCCTGCGCAGCACCGGCGAACGCCTGCCGCTG 
ACCGCCGCGCCGCTGCGCTTCGCGTCCTGGATGGGGGGCGACCGCGACGGCAATCCCAACGTTACCGCCAGC 
GTGACCCGCGAGGTCCTGCTGCTGGCGCGCTGGATGGCCGCCGACCTGTACCTGCGCGATATCGACCGACTG 
GCCGCCGAGCTGTCCATGCAGCAGGCCTCGCCGCAGCTGCTGGCGCGGGTCGGCGATAGCGCCGAGCCCTAT 
CGCGCCCTGCTCAAGCAATTGCGCGAGCGCCTGCGCGTGACCCGCAACTGGACCCACCAGGCCCTGGCCGGC 
GAGGTGCCGGCCGCGGAGGGCGTCCTGGAGCACAACCGCGACCTGGTCGAGCCGCTGCAACTGTGCCACGAG 
TCGCTGCACGCCTGCGGCATGGGCGTGATCGCCGATGGCGCGTTGCTCGACTGCCTGCGCCGCGCCGCCACC 
TTCGGCCTGTTCCTGGTACGCCTGGACGTGCGCCAGGACTCGGCCCGCCATGCCGCCGCGCTGAGCGAGATC 
ACCGAGTACCTGGAGCTGGGCAGCTACGACGAGTGGGACGAGAAGACCCGCCTGGAATTCCTTCTCGAGGAA 
CTGAACAGCCGCCGCCCCCTGCTGCCGGCGCACTACCAGCCTTCGGCGGATACCGCCGAGGTGCTCGCCACC 
TGTCGGGCGATCGCCGCCGCGCCACCGGCGTCGCTGGGTTCCTACGTGATCTCGATGGCCGGCCAGCCGTCC 
GACGTGCTGGCAGTGCAACTACTGCTCAAGGAAAGCGGCGTCGACTGGCCGATGCGCGTGGTGCCGCTGTTC 
GAGACCCTCGACGACCTGGACAACGCCGGCCCCTGCATGGAGCGCCTGCTGACGCTGCCGGGCTACCGTTCG 
CGGCTCTCCGGGGTGCAGGAAGTGATGATCGGCTACTCCGATTCGGCCAGGGACGCCGGTACTCTCACGGCG 
GCCTGGGCGCAGTACCGGGCGCAGGAGAAACTGGTGGAGATCTGCCGCCAGCAAGAGGTCGAGCTGCTGCTC 
TTCCATGGCCGCGGCGGCACCGTCGGCCGCGGCGGCGGGCCGGCCCACGCGGCGATCCTGTCGCAGCCACCG 
GGTTCGGTGGCCGGGCGCTTCCGGGTCACCGAACAGGGCGAGATGATCCGCTTCAAGTTCGGCTTGCCGGAC 
ATCGCCGAACAGAACCTCAATCTCTACCTCGCCGCAGTGCTGGAGGCGACCCTGATGCCGCCGCCGGCTCCC 
GAGCCGGCCTGGCGCGCGCAGATGGACCGCCTGGCGAAGGACGCGCTGCTGGCCTACCGCAGGGTGGTGCGC 
GACGATCCGCAGTTCGTCGAGTATTTCCGCCTGGCCACGCCCGAGCAGGAACTGGGCCGTCTGCCGCTGGGC 
AGCCGCCCGGCCAAGCGCCGCGAAGGCGGGGTGGAGAGCCTGCGGGCGATTCCCTGGATCTTCGCCTGGACC 
CAGACGCGCCTGATGCTGCCGGCATGGCTTGGCTGGGAGACCGCCTTGCTGAACGCCATCGAGCGTGGCGAG 
GGCGCGCTGCTCGGGCAGATGCGCGAGCAATGGCCGTTCTTCACCACCCGCATCGACATGCTGGAAATGGTC 
CTGGCCAAGGCCGACGCGGATATCGCGCGGCTCTACGACGAACGTCTAGTGCCGCTCGAGCTGCGTCCGCTG 
GGGCGTCGTTTACGCGACCTATTGTCGCAGGCGGTGCGCGTGGTGCTGGGACTGACCGGGCAGTCGCTGCTG 
CTCGCCCACGCCAGCGAGACCCGCGAGTCGATCAGCGTGCGCAACAGCTATCTAGACCCGCTGCACCTGCTC 
CAGGCTGAGCTTCTGGCCCGTTCGCGGCGCTGCCAGGGCGACGCCTGCGGCGGCCTGGAGCAGGCGCTGCTG 
GTGACGGTCGCGGGGGTCGCCGCCGGACTGCGCAACACCGGATGA