ViewGene
Locus Name:
GeneID:
Location:

GeneID:1359
TrivialName: PA14_70670
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_70670
PAO1 Ortholog Locus:PA5353
Sequence Length:1227
Protein Length:408
Start: 6294033
Stop: 6292807
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG08738.1|AE004947_8 (AE004947) glycolate oxidase subunit GlcF [Pseudomonas aeruginosa PAO1]
Identities = 404/408 (99%), Positives = 405/408 (99%)
Sequence:
ATGCAAACCAACCTCTGCGATTCCGCCCGGCAACGCGCCGAGGCCGCCGAAGCCGAGCGCATCCTGCGCAGC
TGCGTGCACTGCGGTTTCTGCAACGCCACCTGCCCGACCTACCAGGAGCTGGGCGACGAACTCGACGGCCCG
CGCGGACGGATCTACCTGATCAAGCAGTTCCTCGAGCGCGACGAGGCCAGCGAGCGCACCCGCCTGCACCTC
GATCGCTGCCTGAGCTGCCGCAACTGCGAAACCACCTGCCCATCCGGAGTCGAGTACCACAAGCTGCTGGAT
ATCGGCCGCGACCTCCTCGCCGAACGCCTGCCGCGCGGCTTCCGGCAACGCCTGCTGCGCGACGTGCTGCGC
CTGCTGGCCCCGCGCCCGGGGTTGTTCGGCGTGCTGGCCGGCGCTGCCGGGCGGCTGAGGCCTCTGCTGCCT
GAGGCGATGCAGGGCAAGCTGCCACGTCGGGTCGCGGCGCCGGGCAGGCGGCCGCAGCCCCGGCATGCGCGG
CGCGTGCTGCTGCTCGAAGGTTGCGTGCAGCCGGCGCTGTCGCCGAATACCAACGCCGCCGCCGCGCGGGTT
CTGGACCGCCTGGGAATCAGCGTGACGCCGGCGGCCGCGGCCGGCTGCTGCGGCGCGCTGGATTTCCACCTG
GATGCGCAGGAGCGCGGCCGCCAGCGCGCGCGGCGCAATATCGACGCCTGGTGGCCGTTGCTGCGCGAGGGT
GCCGAGGCCATCGTGCAGACCGCCAGCGGCTGCGGCGCCTTCGTCAAGGACTACGCCTACCTGCTGCGCGAC
GACCCACGGTACGCGGACAAGGCGCGCGAGGTCGCCGCCGCTGCCCGCGACCTGGTGGAAGTGCTTCGCGAC
GAGCCGCTGGAGTCCCTCGGCCTGCGCTGCGACCAGCGCCTGGCATTCCACTGCCCATGCACACTGCAGCAC
GCGCAACGACTGGGCGGGGCCGTGGAGGCGGTGCTGCGGCGACTGGGCTTCCATCTCACCGAGGTGGCCGAC
GGTCATCTCTGCTGCGGCTCCGCCGGCACCTACTCGCTGACCCAGCCGGCGCTGTCCCGGCGTCTGCGCGAC
AACCGCCTGGACGCCCTGGAAAGCGGCGCGCCGGATGTCATCGCCACGGCCAACGTCGGTTGCCAGGCCCAT
CTCGACGGGGCCGGACGGACCCCGGTACGCCACTGGATCGAGCTGCTCGACCAGGCCTTGCCCGGAGGAGAG
TGA
Translation:
MQTNLCDSARQRAEAAEAERILRSCVHCGFCNATCPTYQELGDELDGPRGRIYLIKQFLERDEASERTRLHL
DRCLSCRNCETTCPSGVEYHKLLDIGRDLLAERLPRGFRQRLLRDVLRLLAPRPGLFGVLAGAAGRLRPLLP
EAMQGKLPRRVAAPGRRPQPRHARRVLLLEGCVQPALSPNTNAAAARVLDRLGISVTPAAAAGCCGALDFHL
DAQERGRQRARRNIDAWWPLLREGAEAIVQTASGCGAFVKDYAYLLRDDPRYADKAREVAAAARDLVEVLRD
EPLESLGLRCDQRLAFHCPCTLQHAQRLGGAVEAVLRRLGFHLTEVADGHLCCGSAGTYSLTQPALSRRLRD
NRLDALESGAPDVIATANVGCQAHLDGAGRTPVRHWIELLDQALPGGE*
AnnotationID:1368GeneID:1359
AnnotatorUID: diggins
Modification Date/Time: 2005-03-17 06:06:15
Gene Name:glcF
Confidence Code:2
GeneProduct:glycolate oxidase, iron-sulfur subunit
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(12) Energy metabolism
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:COG0247
Secondary Functional Category(ies):central intermediary metabolism
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAN69342.1|AE016571_8 (AE016788) glycolate oxidase, iron-sulfur subunit [Pseudomonas
           putida KT2440]
          Length = 411

 Score =  604 bits (1557), Expect = e-172
 Identities = 289/404 (71%), Positives = 336/404 (83%)

>gb|AAC76014.1| (AE000380) glycolate oxidase iron-sulfur subunit [Escherichia coli
           K12]
          Length = 761

 Score =  561 bits (1446), Expect = e-159
 Identities = 267/404 (66%), Positives = 319/404 (78%)

>emb|CAD84584.1| (BX321858) glcF; glycolate oxidase (iron-sulfur subunit) protein
           [Nitrosomonas europaea ATCC 19718]
          Length = 419

 Score =  452 bits (1164), Expect = e-126
 Identities = 234/421 (55%), Positives = 284/421 (67%), Gaps = 17/421 (4%)








 
Structural Features:
COG0247, GlpC, Fe-S oxidoreductase [Energy production and conversion]
CD-Length = 388 residues,  99.5% aligned
Score =  240 bits (612), Expect = 3e-64
Identities = 119/399 (29%), Positives = 179/399 (44%), Gaps = 21/399 (5%)








   
Genomic Context:






   
Comment:


















    ReferenceID:2721




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:19866




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:34741




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:1359_cdd.html


    		




 
URL:

















    ReferenceID:35684




  
Author/Investigator(s):
        Pellicer MT, Badia J, Aguilar J, Baldoma L.





  
Title:
        glc locus of Escherichia coli: characterization of genes encoding the subunits of glycolate oxidase and the glc regulator protein.




  
PubMed:
        8606183


MedLine:
        




    
Source:
J Bacteriol. 1996 Apr;178(7):2051-9.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=8606183









           
Homologs By Global Alignment 

           

       



     
Gene ID:1359
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
2468
                           PA5353_tr
                           translation of PA5353
                           408
                           99.01
                           99.26
                           0
                           2129.0
     

     
     
78386
                           gb|AAG08738.1|AE004947_8
                           (AE004947) glycolate oxidase subunit GlcF [Pseudomonas aeruginosa PAO1]
                           408
                           99.01
                           99.26
                           0
                           2129.0
     

     
     
78387
                           gb|AAN69342.1|AE016571_8
                           (AE016788) glycolate oxidase, iron-sulfur subunit [Pseudomonas putida KT2440]
                           411
                           70.55
                           81.99
                           3
                           1550.0
     

     
     
78389
                           gb|AAB02532.1|
                           (L43490) glycolate oxidase subunit, (Fe-S)protein [Escherichia coli]
                           408
                           65.44
                           78.18
                           1
                           1441.0
     

     
     
78391
                           emb|CAD84584.1|
                           (BX321858) glcF; glycolate oxidase (iron-sulfur subunit) protein [Nitrosomonas europaea ATCC 19718]
                           423
                           55.31
                           67.13
                           19
                           1180.0
     

     






      

          
GC ORFID: 44361How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA5353Glimmer Score: 

          
Start: 5557722Stop: 5556496

          
Length: 1227


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA5353 translation of PA5353Homolog Bit Score 824.0

          
Other Homologs:  psyr_15may02_Scaffold9_revised_gene8167 (ORF: BLASTX 597.0), t_PA5353 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGCAAACCAACCTCTGCGATTCCGCCCGGCAACGCGCCGAGGCCGCCGAAGCCGAGCGCATCCTGCGCAGC 
TGCGTGCACTGCGGTTTCTGCAACGCCACCTGCCCGACCTACCAGGAGCTGGGCGACGAACTCGACGGCCCG 
CGCGGACGGATCTACCTGATCAAGCAGTTCCTCGAGCGCGACGAGGCCAGCGAGCGCACCCGCCTGCACCTC 
GATCGCTGCCTGAGCTGCCGCAACTGCGAAACCACCTGCCCATCCGGAGTCGAGTACCACAAGCTGCTGGAT 
ATCGGCCGCGACCTCCTCGCCGAACGCCTGCCGCGCGGCTTCCGGCAACGCCTGCTGCGCGACGTGCTGCGC 
CTGCTGGCCCCGCGCCCGGGGTTGTTCGGCGTGCTGGCCGGCGCTGCCGGGCGGCTGAGGCCTCTGCTGCCT 
GAGGCGATGCAGGGCAAGCTGCCACGTCGGGTCGCGGCGCCGGGCAGGCGGCCGCAGCCCCGGCATGCGCGG 
CGCGTGCTGCTGCTCGAAGGTTGCGTGCAGCCGGCGCTGTCGCCGAATACCAACGCCGCCGCCGCGCGGGTT 
CTGGACCGCCTGGGAATCAGCGTGACGCCGGCGGCCGCGGCCGGCTGCTGCGGCGCGCTGGATTTCCACCTG 
GATGCGCAGGAGCGCGGCCGCCAGCGCGCGCGGCGCAATATCGACGCCTGGTGGCCGTTGCTGCGCGAGGGT 
GCCGAGGCCATCGTGCAGACCGCCAGCGGCTGCGGCGCCTTCGTCAAGGACTACGCCTACCTGCTGCGCGAC 
GACCCACGGTACGCGGACAAGGCGCGCGAGGTCGCCGCCGCTGCCCGCGACCTGGTGGAAGTGCTTCGCGAC 
GAGCCGCTGGAGTCCCTCGGCCTGCGCTGCGACCAGCGCCTGGCATTCCACTGCCCATGCACACTGCAGCAC 
GCGCAACGACTGGGCGGGGCCGTGGAGGCGGTGCTGCGGCGACTGGGCTTCCATCTCACCGAGGTGGCCGAC 
GGTCATCTCTGCTGCGGCTCCGCCGGCACCTACTCGCTGACCCAGCCGGCGCTGTCCCGGCGTCTGCGCGAC 
AACCGCCTGGACGCCCTGGAAAGCGGCGCGCCGGATGTCATCGCCACGGCCAACGTCGGTTGCCAGGCCCAT 
CTCGACGGGGCCGGACGGACCCCGGTACGCCACTGGATCGAGCTGCTCGACCAGGCCTTGCCCGGAGGAGAG 
TGA