ViewGene
Locus Name:
GeneID:
Location:

GeneID:1351
TrivialName: PA14_66680
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_66680
PAO1 Ortholog Locus:PA5046
Sequence Length:1269
Protein Length:422
Start: 5956123
Stop: 5954855
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG08431.1|AE004918_1 (AE004918) malic enzyme [Pseudomonas aeruginosa PAO1]
Identities = 421/422 (99%), Positives = 422/422 (100%)
Sequence:
ATGTCCGATCTTAAAACCGCTGCCCTCGAATATCACGCACAACCCCGTCCGGGAAAACTGAGTGTCGAGCTG
ACCAAACCCACCGCTACCGCCCGGGACCTGTCCCTGGCCTACAGCCCGGGTGTCGCCGAGCCGGTGCGTGAA
ATCGCCCGCGATGCCGAGCTGGCCTATCAGTACACCGGCAAGGGCAACCTGGTGGCAGTGATTTCCGACGGC
ACCGCGATTCTCGGCCTGGGTAACCTCGGCCCGCTGGCCTCCAAGCCGGTGATGGAAGGCAAGGGTGTGCTG
TTCAAGCGCTTCGCCGGCATCGACGTGTTCGACATCGAGGTCGACGCCGAGAGCCCGCAGGCTTTCATCGAT
ACCGTCAAGCGTATTTCCATCACCTTCGGCGGTATCAACCTCGAGGACATCAAGGCTCCCGAGTGTTTCGAG
ATCGAGCGCGCGCTGATCGAACAGTGCGACATCCCGGTGTTCCACGATGACCAGCACGGCACCGCCATTGTG
ACCGCGGCGGGCATGCTCAACGCGCTGGAACTGGCCGGCAAGAAGCTGGAAGAAGCGAAGATCGTCTGCCTC
GGCGCCGGCGCTGCCGCCATCTCCTGCATGAAGCTGCTGGTGAGCATGGGCGCCAAGGTCGAGAACATCTAC
ATGATCGACCGCAAGGGCGTGATCCACGCCGGTCGCGACGATCTGAACCAGTACAAGTCGGTGTTCGCCACC
GAGACCGACAAGCGTACCCTGGCCGACGCCCTGGAGGGCGCCGACGTGTTCGTCGGCCTGTCCGGCGCCAAC
CTGCTGGAGCCGGAAGACCTCAAGCGCATGGCGCCGAACCCGATCGTGTTCGCCTGCTCGAACCCGGACCCG
GAGATCAAGCCGGAACTGGCCCATGCCACCCGCAACGACGTGATCATGGCTACCGGACGTTCCGACTATCCG
AACCAGGTCAACAACGTGCTCGGTTTCCCCTTCATCTTCCGCGGTGCCCTGGACGTACGTGCCACGCGCATC
AACGAAGAGATGAAGATCGCCGCCGCCCTGGCCCTGCGCGACCTGGCCAAGCAGCCGGTCCCGGAGGACGTC
TGCGCCGCCTATGGCGTGGACAAGCTGGAATTCGGCCGCGAGTACATCATTCCGAAGCCGATGGACAAGCGC
CTGATCACCGTGGTTTCCGATGCCGTGGCCAAGGCTGCCATCGAGACCGGCGTGGCTACCCTGCCGTATCCG
AAGAACTATCCGCTGAAATCGGTGGACGAAGTCTTCAACGGCTGA
Translation:
MSDLKTAALEYHAQPRPGKLSVELTKPTATARDLSLAYSPGVAEPVREIARDAELAYQYTGKGNLVAVISDG
TAILGLGNLGPLASKPVMEGKGVLFKRFAGIDVFDIEVDAESPQAFIDTVKRISITFGGINLEDIKAPECFE
IERALIEQCDIPVFHDDQHGTAIVTAAGMLNALELAGKKLEEAKIVCLGAGAAAISCMKLLVSMGAKVENIY
MIDRKGVIHAGRDDLNQYKSVFATETDKRTLADALEGADVFVGLSGANLLEPEDLKRMAPNPIVFACSNPDP
EIKPELAHATRNDVIMATGRSDYPNQVNNVLGFPFIFRGALDVRATRINEEMKIAAALALRDLAKQPVPEDV
CAAYGVDKLEFGREYIIPKPMDKRLITVVSDAVAKAAIETGVATLPYPKNYPLKSVDEVFNG*
AnnotationID:1360GeneID:1351
AnnotatorUID: diggins
Modification Date/Time: 2005-04-26 11:11:59
Gene Name:maeB
Confidence Code:2
GeneProduct:malic enzyme
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(8) Central intermediary metabolism
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:COG0281,pfam03949,pfam00390
Secondary Functional Category(ies):energy metabolism
EC Number:1.1.1.38
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAN70650.1|AE016709_2 (AE016793) malic enzyme [Pseudomonas putida KT2440]
          Length = 422

 Score =  760 bits (1962), Expect = 0.0
 Identities = 383/422 (90%), Positives = 403/422 (95%)

>gb|AAO58561.1| (AE016874) malic enzyme family protein [Pseudomonas syringae pv.
           tomato str. DC3000]
          Length = 422

 Score =  734 bits (1896), Expect = 0.0
 Identities = 369/422 (87%), Positives = 397/422 (94%)

>gb|AAO09812.1|AE016801_131 (AE016801) Malic enzyme [Vibrio vulnificus CMCP6]
          Length = 425

 Score =  583 bits (1504), Expect = e-166
 Identities = 287/408 (70%), Positives = 338/408 (82%)








 
Structural Features:
COG0281, SfcA, Malic enzyme [Energy production and conversion]
CD-Length = 432 residues,  94.7% aligned
Score =  544 bits (1403), Expect = 8e-156
Identities = 234/424 (55%), Positives = 282/424 (66%), Gaps = 19/424 (4%)

pfam03949, Malic_M, Malic enzyme, NAD binding domain.
CD-Length = 256 residues, 100.0% aligned
Score =  233 bits (596), Expect = 3e-62
Identities = 120/269 (44%), Positives = 146/269 (54%), Gaps = 45/269 (16%)

pfam00390, malic, Malic enzyme, N-terminal domain.
CD-Length = 186 residues, 100.0% aligned
Score =  136 bits (343), Expect = 5e-33
Identities = 68/189 (35%), Positives = 87/189 (46%), Gaps = 48/189 (25%)






   
Genomic Context:






   
Comment:


















    ReferenceID:2705




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:19850




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:35034




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:1351_cdd.html


    		




 
URL:

















    ReferenceID:35842




  
Author/Investigator(s):
        Kobayashi K, Doi S, Negoro S, Urabe I, Okada H.





  
Title:
        Structure and properties of malic enzyme from Bacillus stearothermophilus.




  
PubMed:
        2644282


MedLine:
        




    
Source:
J Biol Chem. 1989 Feb 25;264(6):3200-5.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=89123439&dopt=Abstract









           
Homologs By Global Alignment 

           

       



     
Gene ID:1351
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
2458
                           PA5046_tr
                           translation of PA5046
                           422
                           99.76
                           99.99
                           0
                           2138.0
     

     
     
77972
                           gb|AAG08431.1|AE004918_1
                           (AE004918) malic enzyme [Pseudomonas aeruginosa PAO1]
                           422
                           99.76
                           99.99
                           0
                           2138.0
     

     
     
77973
                           gb|AAN70650.1|AE016709_2
                           (AE016793) malic enzyme [Pseudomonas putida KT2440]
                           422
                           90.75
                           95.49
                           0
                           1962.0
     

     
     
77974
                           gb|AAO58561.1|
                           (AE016874) malic enzyme family protein [Pseudomonas syringae pv. tomato str. DC3000]
                           422
                           87.44
                           94.07
                           0
                           1896.0
     

     
     
77975
                           dbj|BAC61030.1|
                           (AP005082) putative malate oxidoreductase [Vibrio parahaemolyticus]
                           433
                           67.20
                           79.90
                           19
                           1525.0
     

     






      

          
GC ORFID: 44353How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA5046Glimmer Score: 

          
Start: 5219806Stop: 5218538

          
Length: 1269


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA5046 translation of PA5046Homolog Bit Score 828.0

          
Other Homologs:  psyr_15may02_Scaffold2_revised_gene3287 (ORF: BLASTX 726.0), t_PA5046 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGTCCGATCTTAAAACCGCTGCCCTCGAATATCACGCACAACCCCGTCCGGGAAAACTGAGTGTCGAGCTG 
ACCAAACCCACCGCTACCGCCCGGGACCTGTCCCTGGCCTACAGCCCGGGTGTCGCCGAGCCGGTGCGTGAA 
ATCGCCCGCGATGCCGAGCTGGCCTATCAGTACACCGGCAAGGGCAACCTGGTGGCAGTGATTTCCGACGGC 
ACCGCGATTCTCGGCCTGGGTAACCTCGGCCCGCTGGCCTCCAAGCCGGTGATGGAAGGCAAGGGTGTGCTG 
TTCAAGCGCTTCGCCGGCATCGACGTGTTCGACATCGAGGTCGACGCCGAGAGCCCGCAGGCTTTCATCGAT 
ACCGTCAAGCGTATTTCCATCACCTTCGGCGGTATCAACCTCGAGGACATCAAGGCTCCCGAGTGTTTCGAG 
ATCGAGCGCGCGCTGATCGAACAGTGCGACATCCCGGTGTTCCACGATGACCAGCACGGCACCGCCATTGTG 
ACCGCGGCGGGCATGCTCAACGCGCTGGAACTGGCCGGCAAGAAGCTGGAAGAAGCGAAGATCGTCTGCCTC 
GGCGCCGGCGCTGCCGCCATCTCCTGCATGAAGCTGCTGGTGAGCATGGGCGCCAAGGTCGAGAACATCTAC 
ATGATCGACCGCAAGGGCGTGATCCACGCCGGTCGCGACGATCTGAACCAGTACAAGTCGGTGTTCGCCACC 
GAGACCGACAAGCGTACCCTGGCCGACGCCCTGGAGGGCGCCGACGTGTTCGTCGGCCTGTCCGGCGCCAAC 
CTGCTGGAGCCGGAAGACCTCAAGCGCATGGCGCCGAACCCGATCGTGTTCGCCTGCTCGAACCCGGACCCG 
GAGATCAAGCCGGAACTGGCCCATGCCACCCGCAACGACGTGATCATGGCTACCGGACGTTCCGACTATCCG 
AACCAGGTCAACAACGTGCTCGGTTTCCCCTTCATCTTCCGCGGTGCCCTGGACGTACGTGCCACGCGCATC 
AACGAAGAGATGAAGATCGCCGCCGCCCTGGCCCTGCGCGACCTGGCCAAGCAGCCGGTCCCGGAGGACGTC 
TGCGCCGCCTATGGCGTGGACAAGCTGGAATTCGGCCGCGAGTACATCATTCCGAAGCCGATGGACAAGCGC 
CTGATCACCGTGGTTTCCGATGCCGTGGCCAAGGCTGCCATCGAGACCGGCGTGGCTACCCTGCCGTATCCG 
AAGAACTATCCGCTGAAATCGGTGGACGAAGTCTTCAACGGCTGA