ViewGene
Locus Name:
GeneID:
Location:

GeneID:134
TrivialName: PA14_43350
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_43350
PAO1 Ortholog Locus:PA1636
Sequence Length:2658
Protein Length:885
Start: 3862963
Stop: 3860306
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG05025.1|AE004591_5 (AE004591) two-component sensor KdpD [Pseudomonas aeruginosa PAO1]
Identities = 883/885 (99%), Positives = 885/885 (100%)
Sequence:
ATGACCGACACCGCACGCGCCGACGCGCTGCTGGCCGACCTGCCCGCTGACGGGCGCGGCCGCCTGAAAGTC
TTTCTCGGCGCGGCGCCGGGGGTGGGCAAGACCTTCGCCATGCTGCAGGCCGCCCAGGCCCAGTTGCGCCAG
GGCGTGAAACTGCGCGCCGGGGTGGTCGAGACCCACTCGCGGGCGGAAACCGAGTCCCTCCTCGCAGGCGTG
CCGCAGCAGCCGCTGCGCAGCCTCGAGTATCGAGGGATGAAACTGCAGGAAATGGACCTCGACGGTCTCCTC
GCCGACCCGCCGGCGCTGGCCCTGGTCGACGAACTGGCCCACAGCAACGTTCCCGGCAGCCGCCACGCCAAG
CGCTGGCAGGACATCCAGGAGTTGCTGGCGGCGGGAATCGACGTCTACACAACGGTCAACGTCCAGCACCTG
GAGAGCCTCAACGACCAGGTCCGCGACATCACCGGCGTGCAGGTCCGCGAGACGGTGCCGGACTGGGTGTTG
CAGGAGGCCTACGAGATCGTCCTCATCGACCTGCCGCCGCGCGAGCTGCTGGAGCGCCTGCGCGAGGGCAAG
GTCTACGTGCCGGAACAGGCGCGGGCGGCGATCGATGCGTTCTTCTCGCAGACCAACCTCACCGCGCTGCGT
GAACTGGCGATGCAGACCGCCGCCGCGCGGGTCGACGCCGACCTCAACCACCGCTACCGCCAGCGCGGCGAG
GAGGCGCCGGCGTTGCGCGGGCGGCTGCTGGTGGGCATCGACGGTGACGAACAGGCCGAACGCCTGGTGCGC
CACGCCTGCCGGGTCGCCGAACGTCGGCACCTGCCCTGGAGCGTGGTACACGTCGATACCGGCGGCTTGCGC
GGCGAGCAGGCGCGGATGCGCCTGCAGGGTGCGCAGCAACTGGCCGAGCGGCTCGGCGGCGAGATCGTCGTG
CTGCGCGCCGGCGAGGTGGCGCGGACCCTCCTGCAACATGCCCGCGAGCGCCGCGCCAGCCTGGTCCTGGTT
GGCCAGTCGCGGCGCCGCTGGTACCGCCGGGCCTTCGGCGGTGGTCTCGCCGGGCGCCTGCTGCGCGACGGC
CAGGGCCTGGAGATCAGTGCCCTGGACAGCGAGGCCGACCTGCGCCCGGCGGAACCGCGGCCACGCTATCCG
GTGGTGTGGAACGACTATCTGCTGGCGCTGCTGGCGACCGCCCTGGCCAGCGGCCTGGCCTGGGTCGTGGCG
CACCTGCTGGAACTGCCGAACATCTCCCTGGTGTTCCTGATGGCGGTGCTGCTGGTGGCAGTGCGCAGCAGC
CTGATGCCGGCACTGCTGTGCGCGGTGGCGTCGTTCCTGGCCTATGACTTCCTGTTCATCCCACCGCATTTT
TCCTTCGCCATCCATCGCCATGAAGACCTGCTGAGCCTGGCGTTCTTCCTGTTCATGGCCGGCCTCACCGGC
AACCTGGCGGCACGCCAGCGGCGCCAGCTGGAGGCGCTGCGTGATACCCAGGAGGAAACCAGCCTGCTGCTG
GAGCTGTCGCGCAAGCTCACCGCCGCCACCGACCGCCAGGCGGTGCTGGCCGCCGCCGCCCAGCAGTTCGAC
GACTGGAAGGACGTCGAGGTCTGCCTGCTCGGTCAGGTCAATGGCGAGTGGAAGACCGAGGCCGGCGTGCAG
CGGCAACTCGCCGACCAGGAACGGGTCGCCGCCGACTGGGCCTGGGCCCACGACCAGCCGGCCGGCCTCGGT
ACTGGCACGCTGCCCGGAGGACGCTGGTGGTGGTGGCCGCTGTCGGCGGAAGAGGGGCCGCTGGCACTGCTC
GGCGTCGCGCCGCTGGACGGCCAGCCGCTGAGTCCGCAGCGCCGGCGCCTGCTCGCCGCCCTCGGCCAGCCC
CTGGCCCAGGCCCTGGCCCGTGCGCGCCTGGCCGAGGACCTGGAGGCCGCGCGCCTGCACGGCGAGACCGAG
CAACTGCGCAGCGCCTTGCTCGCCTCGGTGTCCCACGACCTGCGCACGCCGCTGACGGCCATGCGCGGTTCC
ATCGACAGCCTGCTGGCGCTGGGCGAGGCGATCCCGCCGGCGGATCGTCGCGAACTGCTGGAAAGCACCCGC
GACGAGGCCGAGCGACTGGACCGCTATATCCAGAACCTGCTCGACATGACCCGTCTTGGCCACGGTGGCCTG
AAGCTGGCGCGCGACTGGGTGGCGCCGGGCGACATCGTCGGCAGCGCGCTGAACCGCCTGCGCGCGGTGCTG
GCGCCGTACCGGGTGCAGGCGATCGTGGCCAACGACCTGCCGTTGCTCTACGTGCATGCGGCGCTGATCGAA
CAGGCGCTGATCAACGTGCTGGAAAATGCCGCGCGCTTCTCGCCGAGCCACGGCCGGTTGCGCATCGAGGTC
AGCCAGGATGCCGCCGAGCTGCGTTTCGCGGTGAGCGACGAGGGGCCGGGGATTCCCGAGGCCGAGCGGGAG
AAGATCTTCGACATGTTCTACACCGCTGCCCGTGGCGACCGCGGTGGCCAGGGCACCGGCCTGGGCCTGGCG
ATCTGCCAGGGCATGATCGGCGCCCACGGCGGGCGCATCAGCGTCGGCGAAGGCCTCGACGGTCGCGGCACC
ACGCTGGTCCTGCACCTGCCGCTGCATCCGCAGCCGGACCTCGGGCAACTGGAGGGCGACGAATGA
Translation:
MTDTARADALLADLPADGRGRLKVFLGAAPGVGKTFAMLQAAQAQLRQGVKLRAGVVETHSRAETESLLAGV
PQQPLRSLEYRGMKLQEMDLDGLLADPPALALVDELAHSNVPGSRHAKRWQDIQELLAAGIDVYTTVNVQHL
ESLNDQVRDITGVQVRETVPDWVLQEAYEIVLIDLPPRELLERLREGKVYVPEQARAAIDAFFSQTNLTALR
ELAMQTAAARVDADLNHRYRQRGEEAPALRGRLLVGIDGDEQAERLVRHACRVAERRHLPWSVVHVDTGGLR
GEQARMRLQGAQQLAERLGGEIVVLRAGEVARTLLQHARERRASLVLVGQSRRRWYRRAFGGGLAGRLLRDG
QGLEISALDSEADLRPAEPRPRYPVVWNDYLLALLATALASGLAWVVAHLLELPNISLVFLMAVLLVAVRSS
LMPALLCAVASFLAYDFLFIPPHFSFAIHRHEDLLSLAFFLFMAGLTGNLAARQRRQLEALRDTQEETSLLL
ELSRKLTAATDRQAVLAAAAQQFDDWKDVEVCLLGQVNGEWKTEAGVQRQLADQERVAADWAWAHDQPAGLG
TGTLPGGRWWWWPLSAEEGPLALLGVAPLDGQPLSPQRRRLLAALGQPLAQALARARLAEDLEAARLHGETE
QLRSALLASVSHDLRTPLTAMRGSIDSLLALGEAIPPADRRELLESTRDEAERLDRYIQNLLDMTRLGHGGL
KLARDWVAPGDIVGSALNRLRAVLAPYRVQAIVANDLPLLYVHAALIEQALINVLENAARFSPSHGRLRIEV
SQDAAELRFAVSDEGPGIPEAEREKIFDMFYTAARGDRGGQGTGLGLAICQGMIGAHGGRISVGEGLDGRGT
TLVLHLPLHPQPDLGQLEGDE*
AnnotationID:144GeneID:134
AnnotatorUID: diggins
Modification Date/Time: 2005-04-28 07:07:43
Gene Name:kdpD
Confidence Code:2
GeneProduct:two-component sensor KdpD
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(28) Two-component regulatory systems
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:COG2205,pfam02702
Secondary Functional Category(ies):
EC Number:2.7.3.-
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAN69740.1|AE016610_6 (AE016789) sensor protein KdpD [Pseudomonas putida KT2440]
          Length = 884

 Score = 1322 bits (3422), Expect = 0.0
 Identities = 682/880 (77%), Positives = 757/880 (86%), Gaps = 5/880 (0%)

>gb|AAO55761.1| (AE016863) sensor protein KdpD [Pseudomonas syringae pv. tomato
           str. DC3000]
          Length = 887

 Score = 1287 bits (3331), Expect = 0.0
 Identities = 667/880 (75%), Positives = 737/880 (83%), Gaps = 3/880 (0%)

>gb|AAM35648.1| (AE011706) two-component system sensor protein [Xanthomonas
           axonopodis pv. citri str. 306]
          Length = 886

 Score =  764 bits (1974), Expect = 0.0
 Identities = 435/887 (49%), Positives = 569/887 (64%), Gaps = 21/887 (2%)








 
Structural Features:
COG2205, KdpD, Osmosensitive K+ channel histidine kinase 
[Signal transduction mechanisms]
CD-Length = 890 residues,  99.9% aligned
Score = 1032 bits (2669), Expect = 0.0
Identities = 435/891 (48%), Positives = 569/891 (63%), Gaps = 10/891 (1%)

pfam02702, KdpD, Osmosensitive K+ channel His kinase sensor domain.
CD-Length = 209 residues, 100.0% aligned
Score =  330 bits (847), Expect = 5e-91
Identities = 133/209 (63%), Positives = 162/209 (77%)






   
Genomic Context:






   
Comment:


















    ReferenceID:271




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:17413




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:37259




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:134_cdd.html


    		




 
URL:

















    ReferenceID:37884




  
Author/Investigator(s):
        Treuner-Lange A, Kuhn A, Durre P.





  
Title:
        The kdp system of Clostridium acetobutylicum: cloning, sequencing, and transcriptional regulation in response to potassium concentration.




  
PubMed:
        9226259


MedLine:
        




    
Source:
J Bacteriol. 1997 Jul;179(14):4501-12.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=9226259

















    ReferenceID:37892




  
Author/Investigator(s):
        Walderhaug MO, Polarek JW, Voelkner P, Daniel JM, Hesse JE, Altendorf K, Epstein W.





  
Title:
        KdpD and KdpE, proteins that control expression of the kdpABC operon, are members of the two-component sensor-effector class of regulators.




  
PubMed:
        1532388


MedLine:
        




    
Source:
J Bacteriol. 1992 Apr;174(7):2152-9.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=1532388

















    ReferenceID:37893




  
Author/Investigator(s):
        Polarek JW, Williams G, Epstein W.





  
Title:
        The products of the kdpDE operon are required for expression of the Kdp ATPase of Escherichia coli.




  
PubMed:
        1532387


MedLine:
        




    
Source:
J Bacteriol. 1992 Apr;174(7):2145-51.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=1532387

















    ReferenceID:37895




  
Author/Investigator(s):
        Voelkner P, Puppe W, Altendorf K.





  
Title:
        Characterization of the KdpD protein, the sensor kinase of the K(+)-translocating Kdp system of Escherichia coli.




  
PubMed:
        8223625


MedLine:
        




    
Source:
Eur J Biochem. 1993 Nov 1;217(3):1019-26.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=94039122&dopt=Abstract









           
Homologs By Global Alignment 

           

       



     
Gene ID:134
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
247
                           PA1636_tr
                           translation of PA1636
                           885
                           99.77
                           99.99
                           0
                           4481.0
     

     
     
17270
                           gb|AAG05025.1|AE004591_5
                           (AE004591) two-component sensor KdpD [Pseudomonas aeruginosa PAO1]
                           885
                           99.77
                           99.99
                           0
                           4481.0
     

     
     
17271
                           gb|AAN69740.1|AE016610_6
                           (AE016789) sensor protein KdpD [Pseudomonas putida KT2440]
                           891
                           76.76
                           85.29
                           13
                           3423.0
     

     
     
17272
                           gb|AAO55761.1|
                           (AE016863) sensor protein KdpD [Pseudomonas syringae pv. tomato str. DC3000]
                           890
                           75.16
                           83.14
                           8
                           3332.0
     

     
     
17273
                           gb|AAM35648.1|
                           (AE011706) two-component system sensor protein [Xanthomonas axonopodis pv. citri str. 306]
                           907
                           48.95
                           63.39
                           43
                           1995.0
     

     






      

          
GC ORFID: 43136How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA1636Glimmer Score: 

          
Start: 1090946Stop: 1093603

          
Length: 2658


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA1636 translation of PA1636Homolog Bit Score 1730.0

          
Other Homologs:  psyr_15may02_Scaffold1_revised_gene1037 (ORF: BLASTX 1290.0), t_PA1636 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGACCGACACCGCACGCGCCGACGCGCTGCTGGCCGACCTGCCCGCTGACGGGCGCGGCCGCCTGAAAGTC 
TTTCTCGGCGCGGCGCCGGGGGTGGGCAAGACCTTCGCCATGCTGCAGGCCGCCCAGGCCCAGTTGCGCCAG 
GGCGTGAAACTGCGCGCCGGGGTGGTCGAGACCCACTCGCGGGCGGAAACCGAGTCCCTCCTCGCAGGCGTG 
CCGCAGCAGCCGCTGCGCAGCCTCGAGTATCGAGGGATGAAACTGCAGGAAATGGACCTCGACGGTCTCCTC 
GCCGACCCGCCGGCGCTGGCCCTGGTCGACGAACTGGCCCACAGCAACGTTCCCGGCAGCCGCCACGCCAAG 
CGCTGGCAGGACATCCAGGAGTTGCTGGCGGCGGGAATCGACGTCTACACAACGGTCAACGTCCAGCACCTG 
GAGAGCCTCAACGACCAGGTCCGCGACATCACCGGCGTGCAGGTCCGCGAGACGGTGCCGGACTGGGTGTTG 
CAGGAGGCCTACGAGATCGTCCTCATCGACCTGCCGCCGCGCGAGCTGCTGGAGCGCCTGCGCGAGGGCAAG 
GTCTACGTGCCGGAACAGGCGCGGGCGGCGATCGATGCGTTCTTCTCGCAGACCAACCTCACCGCGCTGCGT 
GAACTGGCGATGCAGACCGCCGCCGCGCGGGTCGACGCCGACCTCAACCACCGCTACCGCCAGCGCGGCGAG 
GAGGCGCCGGCGTTGCGCGGGCGGCTGCTGGTGGGCATCGACGGTGACGAACAGGCCGAACGCCTGGTGCGC 
CACGCCTGCCGGGTCGCCGAACGTCGGCACCTGCCCTGGAGCGTGGTACACGTCGATACCGGCGGCTTGCGC 
GGCGAGCAGGCGCGGATGCGCCTGCAGGGTGCGCAGCAACTGGCCGAGCGGCTCGGCGGCGAGATCGTCGTG 
CTGCGCGCCGGCGAGGTGGCGCGGACCCTCCTGCAACATGCCCGCGAGCGCCGCGCCAGCCTGGTCCTGGTT 
GGCCAGTCGCGGCGCCGCTGGTACCGCCGGGCCTTCGGCGGTGGTCTCGCCGGGCGCCTGCTGCGCGACGGC 
CAGGGCCTGGAGATCAGTGCCCTGGACAGCGAGGCCGACCTGCGCCCGGCGGAACCGCGGCCACGCTATCCG 
GTGGTGTGGAACGACTATCTGCTGGCGCTGCTGGCGACCGCCCTGGCCAGCGGCCTGGCCTGGGTCGTGGCG 
CACCTGCTGGAACTGCCGAACATCTCCCTGGTGTTCCTGATGGCGGTGCTGCTGGTGGCAGTGCGCAGCAGC 
CTGATGCCGGCACTGCTGTGCGCGGTGGCGTCGTTCCTGGCCTATGACTTCCTGTTCATCCCACCGCATTTT 
TCCTTCGCCATCCATCGCCATGAAGACCTGCTGAGCCTGGCGTTCTTCCTGTTCATGGCCGGCCTCACCGGC 
AACCTGGCGGCACGCCAGCGGCGCCAGCTGGAGGCGCTGCGTGATACCCAGGAGGAAACCAGCCTGCTGCTG 
GAGCTGTCGCGCAAGCTCACCGCCGCCACCGACCGCCAGGCGGTGCTGGCCGCCGCCGCCCAGCAGTTCGAC 
GACTGGAAGGACGTCGAGGTCTGCCTGCTCGGTCAGGTCAATGGCGAGTGGAAGACCGAGGCCGGCGTGCAG 
CGGCAACTCGCCGACCAGGAACGGGTCGCCGCCGACTGGGCCTGGGCCCACGACCAGCCGGCCGGCCTCGGT 
ACTGGCACGCTGCCCGGAGGACGCTGGTGGTGGTGGCCGCTGTCGGCGGAAGAGGGGCCGCTGGCACTGCTC 
GGCGTCGCGCCGCTGGACGGCCAGCCGCTGAGTCCGCAGCGCCGGCGCCTGCTCGCCGCCCTCGGCCAGCCC 
CTGGCCCAGGCCCTGGCCCGTGCGCGCCTGGCCGAGGACCTGGAGGCCGCGCGCCTGCACGGCGAGACCGAG 
CAACTGCGCAGCGCCTTGCTCGCCTCGGTGTCCCACGACCTGCGCACGCCGCTGACGGCCATGCGCGGTTCC 
ATCGACAGCCTGCTGGCGCTGGGCGAGGCGATCCCGCCGGCGGATCGTCGCGAACTGCTGGAAAGCACCCGC 
GACGAGGCCGAGCGACTGGACCGCTATATCCAGAACCTGCTCGACATGACCCGTCTTGGCCACGGTGGCCTG 
AAGCTGGCGCGCGACTGGGTGGCGCCGGGCGACATCGTCGGCAGCGCGCTGAACCGCCTGCGCGCGGTGCTG 
GCGCCGTACCGGGTGCAGGCGATCGTGGCCAACGACCTGCCGTTGCTCTACGTGCATGCGGCGCTGATCGAA 
CAGGCGCTGATCAACGTGCTGGAAAATGCCGCGCGCTTCTCGCCGAGCCACGGCCGGTTGCGCATCGAGGTC 
AGCCAGGATGCCGCCGAGCTGCGTTTCGCGGTGAGCGACGAGGGGCCGGGGATTCCCGAGGCCGAGCGGGAG 
AAGATCTTCGACATGTTCTACACCGCTGCCCGTGGCGACCGCGGTGGCCAGGGCACCGGCCTGGGCCTGGCG 
ATCTGCCAGGGCATGATCGGCGCCCACGGCGGGCGCATCAGCGTCGGCGAAGGCCTCGACGGTCGCGGCACC 
ACGCTGGTCCTGCACCTGCCGCTGCATCCGCAGCCGGACCTCGGGCAACTGGAGGGCGACGAATGA