ViewGene
Locus Name:
GeneID:
Location:

GeneID:1330
TrivialName: PA14_43920
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_43920
PAO1 Ortholog Locus:PA1590
Sequence Length:1314
Protein Length:437
Start: 3910964
Stop: 3909651
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG04979.1|AE004587_3 (AE004587) branched chain amino acid transporter [Pseudomonas aeruginosa PAO1]
Identities = 435/437 (99%), Positives = 436/437 (99%)
Sequence:
ATGACTCATTTGAAAGGGTTCGACCTGCTCGCCCTTGGCTTCATGACGTTCGCGCTTTTTCTCGGCGCGGGT
AACATCATCTTTCCTCCCAGTGCCGGCATGGCGGCGGGCGAACACGTATGGAGCGCGGCTTTCGGCTTCCTC
CTTACCGGTGTCGGCCTGCCGTTGCTGACGGTGGTCGCCCTGGCCCGGGTCGGAGGCGGTATTGGCCGCCTG
ACCCAGCCGATCGGGCGGCGCGCGGGAGTAGCGTTTGCCATCGCCGTCTATCTTGCCATCGGCCCGCTGTTC
GCCACCCCGCGTACAGCCGTGGTGTCCTTCGAGATGGGCGTCGCGCCCTTCACCGGGGACGGCGGCGCGCCA
TTGCTCGTCTATACCGTCGCTTACTTCTCCGTGGTTCTGTTCCTGGTGCTGAACCCGGGTCGCCTTGTCGAC
CGGGTCGGAAAGGTCATCACGCCGGTGCTGCTGTCGGCCCTGCTGGTCCTTGGCGGCGCGGCGATCTTCGCG
CCGGCGGGAGAGATCGGCAGCAGTTCCGGCGAGTACCAGAGCGCGCCCCTGGTGCAGGGTTTCCTGCAGGGC
TACCTGACCATGGATACCCTCGGTGCCCTGGTCTTCGGTATCGTCATCGCCACCGCCATTCGCGACCGCGGC
ATCAGCGACAGCCGGCTGGTCACGCGCTATTCCATGATCGCCGGGGTCATCGCCGCCACCGGCCTGTCCCTG
GTCTACCTGGCGCTGTTCTATCTTGGCGCCACCAGCCAGGGCATCGCCGGCGACGCCCAGAATGGCGTACAG
ATCCTCACCGCCTATGTGCAGCAGACCTTTGGCGTTTCCGGCAGCCTGTTGCTGGCGGTGGTCATCACCCTG
GCCTGCCTGACCACGGCGGTAGGGCTGATCACGGCGTGCGGCGAGTTCTTCAGCGACCTGCTGCCGGTTTCG
TACAAGACGGTGGTGATCGTGTTCTCGCTGTTCAGCCTGCTGGTCGCCAACCAGGGACTGACCCAGTTGATC
AGCCTTTCGGTACCGGTTCTGGTGGGACTCTATCCGCTCGCCATCGTGCTGATCGCCCTGAGCCTGTTCGAC
CGGCTCTGGGTGTCGGCCCCGCGGGTGTTCGTGCCGGTGATGATCGTCGCGCTGCTCTTCGGCATCGTCGAC
GGGCTGGGGGCGGCCAAGCTGAACGGGTGGGTGCCGGACGTCTTCGCCAAGCTCCCGCTCGCCGATCAGAGC
CTTGGCTGGCTGCTGCCGGTATCCATTGCGCTGGTCCTGGCAGTCGTTTGCGATCGGCTGCTGGGCAAGCCG
CGCGAAGCGGTCGCCTGA
Translation:
MTHLKGFDLLALGFMTFALFLGAGNIIFPPSAGMAAGEHVWSAAFGFLLTGVGLPLLTVVALARVGGGIGRL
TQPIGRRAGVAFAIAVYLAIGPLFATPRTAVVSFEMGVAPFTGDGGAPLLVYTVAYFSVVLFLVLNPGRLVD
RVGKVITPVLLSALLVLGGAAIFAPAGEIGSSSGEYQSAPLVQGFLQGYLTMDTLGALVFGIVIATAIRDRG
ISDSRLVTRYSMIAGVIAATGLSLVYLALFYLGATSQGIAGDAQNGVQILTAYVQQTFGVSGSLLLAVVITL
ACLTTAVGLITACGEFFSDLLPVSYKTVVIVFSLFSLLVANQGLTQLISLSVPVLVGLYPLAIVLIALSLFD
RLWVSAPRVFVPVMIVALLFGIVDGLGAAKLNGWVPDVFAKLPLADQSLGWLLPVSIALVLAVVCDRLLGKP
REAVA*
AnnotationID:1339GeneID:1330
AnnotatorUID: diggins
Modification Date/Time: 2005-05-17 06:06:39
Gene Name:braB
Confidence Code:1
GeneProduct:branched chain amino acid transporter
Cell Localization:(4) Inner membrane protein
Synonyms:brnQ
Cell Localization Confidence Code:1
MolecularFunction:branched-chain amino acid transport system II carrier protein
Functional Category:(2) Amino acid biosynthesis and metabolism
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:COG1114,pfam05525
Secondary Functional Category(ies):transport of small molecules
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAN69765.1|AE016612_11 (AE016789) branched-chain amino acid transport system III carrier
           protein [Pseudomonas putida KT2440]
          Length = 437

 Score =  496 bits (1278), Expect = e-140
 Identities = 251/437 (57%), Positives = 318/437 (72%)

>gb|AAO55722.1| (AE016863) branched-chain amino acid transport system II carrier
           protein [Pseudomonas syringae pv. tomato str. DC3000]
          Length = 437

 Score =  495 bits (1275), Expect = e-139
 Identities = 253/437 (57%), Positives = 318/437 (72%)

>gb|AAN78989.1|AE016756_172 (AE016756) Branched-chain amino acid transport system II carrier
           protein [Escherichia coli CFT073]
          Length = 439

 Score =  481 bits (1237), Expect = e-135
 Identities = 236/432 (54%), Positives = 317/432 (73%), Gaps = 1/432 (0%)






 
Structural Features:
COG1114, BrnQ, Branched-chain amino acid permeases 
[Amino acid transport and metabolism]
CD-Length = 431 residues, 100.0% aligned
Score =  445 bits (1146), Expect = 5e-126
Identities = 234/431 (54%), Positives = 306/431 (70%), Gaps = 2/431 (0%)

pfam05525, Branch_AA_trans, Branched-chain amino acid transport protein. 
CD-Length = 428 residues, 100.0% aligned
Score =  444 bits (1144), Expect = 8e-126
Identities = 230/428 (53%), Positives = 308/428 (71%), Gaps = 3/428 (0%)






   
Genomic Context:






   
Comment:
other possible gene name: braZ


















    ReferenceID:2663




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:19806




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:37293




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:1330_cdd.html


    		




 
URL:

















    ReferenceID:37905




  
Author/Investigator(s):
        Hoshino T, Kose K, Uratani Y.





  
Title:
        Cloning and nucleotide sequence of the gene braB coding for the sodium-coupled branched-chain amino acid carrier in Pseudomonas aeruginosa PAO.




  
PubMed:
        2111004


MedLine:
        




    
Source:
Mol Gen Genet. 1990 Feb;220(3):461-7.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=2111004









           
Homologs By Global Alignment 

           

       



     
Gene ID:1330
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
2434
                           PA1590_tr
                           translation of PA1590
                           437
                           99.54
                           99.77
                           0
                           2148.0
     

     
     
77516
                           gb|AAG04979.1|AE004587_3
                           (AE004587) branched chain amino acid transporter [Pseudomonas aeruginosa PAO1]
                           437
                           99.54
                           99.77
                           0
                           2148.0
     

     
     
77517
                           emb|CAA35961.1|
                           (X51634) unnamed protein product [Pseudomonas aeruginosa]
                           437
                           99.54
                           99.77
                           0
                           2148.0
     

     
     
77519
                           gb|AAO55722.1|
                           (AE016863) branched-chain amino acid transport system II carrier protein [Pseudomonas syringae pv. tomato str. DC3000]
                           437
                           57.89
                           72.76
                           0
                           1275.0
     

     
     
77518
                           gb|AAN69765.1|AE016612_11
                           (AE016789) branched-chain amino acid transport system III carrier protein [Pseudomonas putida KT2440]
                           437
                           57.43
                           72.76
                           0
                           1278.0
     

     






      

          
GC ORFID: 44332How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA1590Glimmer Score: 

          
Start: 1042945Stop: 1044258

          
Length: 1314


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA1590 translation of PA1590Homolog Bit Score 832.0

          
Other Homologs:  psyr_15may02_Scaffold1_revised_gene1002 (ORF: BLASTX 501.0), t_PA1971 (ORF: BLASTX 479.0), t_PA1590 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGACTCATTTGAAAGGGTTCGACCTGCTCGCCCTTGGCTTCATGACGTTCGCGCTTTTTCTCGGCGCGGGT 
AACATCATCTTTCCTCCCAGTGCCGGCATGGCGGCGGGCGAACACGTATGGAGCGCGGCTTTCGGCTTCCTC 
CTTACCGGTGTCGGCCTGCCGTTGCTGACGGTGGTCGCCCTGGCCCGGGTCGGAGGCGGTATTGGCCGCCTG 
ACCCAGCCGATCGGGCGGCGCGCGGGAGTAGCGTTTGCCATCGCCGTCTATCTTGCCATCGGCCCGCTGTTC 
GCCACCCCGCGTACAGCCGTGGTGTCCTTCGAGATGGGCGTCGCGCCCTTCACCGGGGACGGCGGCGCGCCA 
TTGCTCGTCTATACCGTCGCTTACTTCTCCGTGGTTCTGTTCCTGGTGCTGAACCCGGGTCGCCTTGTCGAC 
CGGGTCGGAAAGGTCATCACGCCGGTGCTGCTGTCGGCCCTGCTGGTCCTTGGCGGCGCGGCGATCTTCGCG 
CCGGCGGGAGAGATCGGCAGCAGTTCCGGCGAGTACCAGAGCGCGCCCCTGGTGCAGGGTTTCCTGCAGGGC 
TACCTGACCATGGATACCCTCGGTGCCCTGGTCTTCGGTATCGTCATCGCCACCGCCATTCGCGACCGCGGC 
ATCAGCGACAGCCGGCTGGTCACGCGCTATTCCATGATCGCCGGGGTCATCGCCGCCACCGGCCTGTCCCTG 
GTCTACCTGGCGCTGTTCTATCTTGGCGCCACCAGCCAGGGCATCGCCGGCGACGCCCAGAATGGCGTACAG 
ATCCTCACCGCCTATGTGCAGCAGACCTTTGGCGTTTCCGGCAGCCTGTTGCTGGCGGTGGTCATCACCCTG 
GCCTGCCTGACCACGGCGGTAGGGCTGATCACGGCGTGCGGCGAGTTCTTCAGCGACCTGCTGCCGGTTTCG 
TACAAGACGGTGGTGATCGTGTTCTCGCTGTTCAGCCTGCTGGTCGCCAACCAGGGACTGACCCAGTTGATC 
AGCCTTTCGGTACCGGTTCTGGTGGGACTCTATCCGCTCGCCATCGTGCTGATCGCCCTGAGCCTGTTCGAC 
CGGCTCTGGGTGTCGGCCCCGCGGGTGTTCGTGCCGGTGATGATCGTCGCGCTGCTCTTCGGCATCGTCGAC 
GGGCTGGGGGCGGCCAAGCTGAACGGGTGGGTGCCGGACGTCTTCGCCAAGCTCCCGCTCGCCGATCAGAGC 
CTTGGCTGGCTGCTGCCGGTATCCATTGCGCTGGTCCTGGCAGTCGTTTGCGATCGGCTGCTGGGCAAGCCG 
CGCGAAGCGGTCGCCTGA