ViewGene
Locus Name:
GeneID:
Location:

GeneID:1325
TrivialName: PA14_41230
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_41230
PAO1 Ortholog Locus:PA1802
Sequence Length:1281
Protein Length:426
Start: 3679862
Stop: 3678582
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG05191.1|AE004606_5 (AE004606) ATP-dependent Clp protease ATP-binding subunit ClpX [Pseudomonas aeruginosa PAO1]
Identities = 426/426 (100%), Positives = 426/426 (100%)
Sequence:
ATGACTGATACCCGCAACGGCGAGGACAACGGCAAGCTGCTGTATTGCTCCTTCTGCGGCAAGAGCCAGCAC
GAAGTGCGCAAGTTGATTGCCGGCCCCTCGGTCTTTATCTGCGACGAGTGTGTCGACCTGTGCAATGACATC
ATCCGCGAGGAGGTGCAGGAAGCGCAGGCGGAAAGTAGTGGGCACAAACTTCCGGCGCCGAAAGAGATCCGC
ACCATCCTCGATCAGTACGTGATCGGGCAGGAGCGGGCCAAGAAGGTTCTGGCGGTAGCGGTATACAACCAC
TACAAGCGCCTCAACCAGCGTGACAAGAAGGACGATATCGAGCTCGGCAAGAGCAACATTCTGATGATCGGC
CCAACCGGCTCGGGCAAGACCCTGCTGGCCGAGACCCTCGCACGTCTGCTGAACGTGCCGTTCACCATCGCC
GATGCGACCACGCTGACGGAAGCGGGCTACGTGGGCGAGGATGTCGAGAACATCATCCAGAAACTCTTGCAG
AAGTGCGATTACGATGTGGAGAAGGCCCAGATGGGCATCGTCTACATCGACGAGATCGACAAGATTTCCCGG
AAATCGGACAACCCCTCCATCACTCGCGACGTTTCCGGCGAGGGTGTGCAGCAGGCCCTGCTGAAGCTCATC
GAGGGCACCGTTGCCTCGGTACCGCCGCAGGGCGGTCGTAAGCACCCGCAGCAGGAGTTCCTGCAGGTCGAT
ACGCGCAACATCCTGTTCATCTGCGGCGGCGCTTTCGCCGGTCTGGAGCGGGTCATCCAGAACCGTTCCGCG
CGCGGCGGCATCGGTTTCAATGCCGAAGTGCGCAGTCAGGAGATGGGCAAGAAGGTCGGCGAGGCGTTCAAG
GAAGTCGAGCCGGAAGATCTCGTCAAGTTCGGTCTTATCCCCGAGTTTGTAGGGCGTTTGCCGGTGATCGCG
ACTCTTGATGAGCTCGACGAGGCTGCGCTCATGCAGATCCTGACCGAGCCGAAGAACGCTCTCACCAAGCAG
TACGCCAAGCTGTTCGAGATGGAAGGCGTCGATCTCGAATTCCGTCCGGACGCACTCAAGGCGGTTGCCCGC
AAGGCGCTCGAACGCAAGACCGGTGCACGTGGCTTGCGTTCAATTCTCGAAGGTATCCTTCTCGATACCATG
TACGAGATTCCCTCCCAGCAGGATGTCAGCAAGGTGGTCATCGATGAGAGTGTCATCGATGGCTCGTCGCAA
CCCCTGATGATCTACGAGAACAGCGAAAAGCCAGCCAAGGCTGCGCCTGAGGCATGA
Translation:
MTDTRNGEDNGKLLYCSFCGKSQHEVRKLIAGPSVFICDECVDLCNDIIREEVQEAQAESSGHKLPAPKEIR
TILDQYVIGQERAKKVLAVAVYNHYKRLNQRDKKDDIELGKSNILMIGPTGSGKTLLAETLARLLNVPFTIA
DATTLTEAGYVGEDVENIIQKLLQKCDYDVEKAQMGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLI
EGTVASVPPQGGRKHPQQEFLQVDTRNILFICGGAFAGLERVIQNRSARGGIGFNAEVRSQEMGKKVGEAFK
EVEPEDLVKFGLIPEFVGRLPVIATLDELDEAALMQILTEPKNALTKQYAKLFEMEGVDLEFRPDALKAVAR
KALERKTGARGLRSILEGILLDTMYEIPSQQDVSKVVIDESVIDGSSQPLMIYENSEKPAKAAPEA*
AnnotationID:1334GeneID:1325
AnnotatorUID: diggins
Modification Date/Time: 2005-03-01 15:03:33
Gene Name:clpX
Confidence Code:1
GeneProduct:ATP-dependent Clp protease ATP-binding subunit ClpX
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(9) Chaperones & heat shock proteins
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:COG1219
Secondary Functional Category(ies):
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAO57194.1| (AE016869) ATP-dependent Clp protease, ATP-binding subunit ClpX
           [Pseudomonas syringae pv. tomato str. DC3000]
          Length = 427

 Score =  788 bits (2034), Expect = 0.0
 Identities = 399/427 (93%), Positives = 417/427 (97%), Gaps = 1/427 (0%)

>gb|AAN67914.1|AE016423_9 (AE016782) ATP-dependent Clp protease, ATP-binding subunit ClpX
           [Pseudomonas putida KT2440]
          Length = 442

 Score =  779 bits (2012), Expect = 0.0
 Identities = 396/427 (92%), Positives = 415/427 (97%), Gaps = 1/427 (0%)

>gb|AAN79032.1|AE016756_215 (AE016756) ATP-dependent Clp protease ATP-binding subunit clpX
           [Escherichia coli CFT073]
          Length = 424

 Score =  633 bits (1632), Expect = 0.0
 Identities = 322/418 (77%), Positives = 358/418 (85%), Gaps = 2/418 (0%)








 
Structural Features:
COG1219, ClpX, ATP-dependent protease Clp, ATPase subunit 
[Posttranslational modification, protein turnover, chaperones]
CD-Length = 408 residues, 100.0% aligned
Score =  725 bits (1872), Expect = 0.0
Identities = 305/408 (74%), Positives = 358/408 (87%)






   
Genomic Context:






   
Comment:


















    ReferenceID:2653




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:19796




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:37131




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:1325_cdd.html


    		




 
URL:

















    ReferenceID:37805




  
Author/Investigator(s):
        Bertani I, Sevo M, Kojic M, Venturi V.





  
Title:
        Role of GacA, LasI, RhlI, Ppk, PsrA, Vfr and ClpXP in the regulation of the stationary-phase sigma factor rpoS/RpoS in Pseudomonas.




  
PubMed:
        12879217


MedLine:
        




    
Source:
Arch Microbiol. 2003 Oct;180(4):264-71. Epub 2003 Jul 23.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=12879217

















    ReferenceID:37806




  
Author/Investigator(s):
        Gerth U, Wipat A, Harwood CR, Carter N, Emmerson PT, Hecker M.





  
Title:
        Sequence and transcriptional analysis of clpX, a class-III heat-shock gene of Bacillus subtilis.




  
PubMed:
        8973311


MedLine:
        




    
Source:
Gene. 1996 Nov 28;181(1-2):77-83.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=8973311

















    ReferenceID:37807




  
Author/Investigator(s):
        Gerth U, Wipat A, Harwood CR, Carter N, Emmerson PT, Hecker M.





  
Title:
        Sequence and transcriptional analysis of clpX, a class-III heat-shock gene of Bacillus subtilis.




  
PubMed:
        8973311


MedLine:
        




    
Source:
Gene. 1996 Nov 28;181(1-2):77-83.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=8973311









           
Homologs By Global Alignment 

           

       



     
Gene ID:1325
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
2418
                           PA1802_tr
                           translation of PA1802
                           426
                           99.99
                           99.99
                           0
                           2153.0
     

     
     
76952
                           gb|AAG05191.1|AE004606_5
                           (AE004606) ATP-dependent Clp protease ATP-binding subunit ClpX [Pseudomonas aeruginosa PAO1]
                           426
                           99.99
                           99.99
                           0
                           2153.0
     

     
     
76953
                           gb|AAO57194.1|
                           (AE016869) ATP-dependent Clp protease, ATP-binding subunit ClpX [Pseudomonas syringae pv. tomato str. DC3000]
                           427
                           93.44
                           97.65
                           1
                           2036.0
     

     
     
76954
                           gb|AAN67914.1|AE016423_9
                           (AE016782) ATP-dependent Clp protease, ATP-binding subunit ClpX [Pseudomonas putida KT2440]
                           442
                           89.59
                           93.89
                           16
                           2014.0
     

     
     
76955
                           gb|AAM35957.1|
                           (AE011737) ATP-dependent Clp protease ATP binding subunit [Xanthomonas axonopodis pv. citri str. 306]
                           429
                           77.85
                           89.27
                           4
                           1720.0
     

     






      

          
GC ORFID: 44327How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA1802Glimmer Score: 

          
Start: 1274047Stop: 1275327

          
Length: 1281


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA1802 translation of PA1802Homolog Bit Score 833.0

          
Other Homologs:  psyr_15may02_Scaffold1_revised_gene777 (ORF: BLASTX 786.0), t_PA1802 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGACTGATACCCGCAACGGCGAGGACAACGGCAAGCTGCTGTATTGCTCCTTCTGCGGCAAGAGCCAGCAC 
GAAGTGCGCAAGTTGATTGCCGGCCCCTCGGTCTTTATCTGCGACGAGTGTGTCGACCTGTGCAATGACATC 
ATCCGCGAGGAGGTGCAGGAAGCGCAGGCGGAAAGTAGTGGGCACAAACTTCCGGCGCCGAAAGAGATCCGC 
ACCATCCTCGATCAGTACGTGATCGGGCAGGAGCGGGCCAAGAAGGTTCTGGCGGTAGCGGTATACAACCAC 
TACAAGCGCCTCAACCAGCGTGACAAGAAGGACGATATCGAGCTCGGCAAGAGCAACATTCTGATGATCGGC 
CCAACCGGCTCGGGCAAGACCCTGCTGGCCGAGACCCTCGCACGTCTGCTGAACGTGCCGTTCACCATCGCC 
GATGCGACCACGCTGACGGAAGCGGGCTACGTGGGCGAGGATGTCGAGAACATCATCCAGAAACTCTTGCAG 
AAGTGCGATTACGATGTGGAGAAGGCCCAGATGGGCATCGTCTACATCGACGAGATCGACAAGATTTCCCGG 
AAATCGGACAACCCCTCCATCACTCGCGACGTTTCCGGCGAGGGTGTGCAGCAGGCCCTGCTGAAGCTCATC 
GAGGGCACCGTTGCCTCGGTACCGCCGCAGGGCGGTCGTAAGCACCCGCAGCAGGAGTTCCTGCAGGTCGAT 
ACGCGCAACATCCTGTTCATCTGCGGCGGCGCTTTCGCCGGTCTGGAGCGGGTCATCCAGAACCGTTCCGCG 
CGCGGCGGCATCGGTTTCAATGCCGAAGTGCGCAGTCAGGAGATGGGCAAGAAGGTCGGCGAGGCGTTCAAG 
GAAGTCGAGCCGGAAGATCTCGTCAAGTTCGGTCTTATCCCCGAGTTTGTAGGGCGTTTGCCGGTGATCGCG 
ACTCTTGATGAGCTCGACGAGGCTGCGCTCATGCAGATCCTGACCGAGCCGAAGAACGCTCTCACCAAGCAG 
TACGCCAAGCTGTTCGAGATGGAAGGCGTCGATCTCGAATTCCGTCCGGACGCACTCAAGGCGGTTGCCCGC 
AAGGCGCTCGAACGCAAGACCGGTGCACGTGGCTTGCGTTCAATTCTCGAAGGTATCCTTCTCGATACCATG 
TACGAGATTCCCTCCCAGCAGGATGTCAGCAAGGTGGTCATCGATGAGAGTGTCATCGATGGCTCGTCGCAA 
CCCCTGATGATCTACGAGAACAGCGAAAAGCCAGCCAAGGCTGCGCCTGAGGCATGA