ViewGene
Locus Name:
GeneID:
Location:

GeneID:132
TrivialName: PA14_41470
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_41470
PAO1 Ortholog Locus:PA1787
Sequence Length:2610
Protein Length:869
Start: 3697030
Stop: 3699639
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG05176.1|AE004604_6 (AE004604) aconitate hydratase 2 [Pseudomonas aeruginosa PAO1]
Identities = 869/869 (100%), Positives = 869/869 (100%)
Sequence:
GTGCTTGAAGCCTACCGCAAACACGTAGAAGAGCGTGCCGCCCAGGGTGTCGTGCCCCAACCGCTGAACGCC
GAACAGACCGCAGGCCTGGTCGAGCTGCTGAAGAACCCGCCGGCCGGCGAAGAAGAATTCCTCCTTGACCTG
ATCACCAACCGCGTACCGCCGGGCGTCGACGAAGCCGCCTACGTCAAGGCCGGATTCCTTTCCGCCATCGTC
AAGGGCGAAGCCACCTCCCCCCTTATCGACAAGCAACGTGCCGCCGAGCTGCTGGGCACCATGCAGGGCGGC
TACAACATCGCCACCCTGGTCGAACTGCTGGACGACGCCGAACTGGCCAACACCGCCGCCGAACAGCTGAAG
CACACCCTGCTGATGTTCGACGCCTTCCACGACGTCGCCGAGCGCGCCAAGAAGGGCAATGCCGCGGCCAAG
TCCGTGCTGCAATCCTGGGCCGATGGCGAGTGGTTCAAGGCCAAGCCGGAAGTCCCGGAAAAGCTGACTCTC
ACCGTATTCAAGGTCCCCGGCGAAACCAACACCGACGACCTCTCCCCGGCCCCCGACGCCTGGTCGCGCCCG
GACATCCCGCTGCACGCCCTGGCCATGCTGAAGATGGCTCGCGACGGCATCGAGCCGGTCCAGCCGGGTTCG
GTCGGTCCGCTGAAGCAGATCGAAGCGGTCAAGGCCAAGGGCTTCCCGGTCGCCTACGTCGGCGACGTGGTC
GGCACCGGTTCCTCGCGCAAATCCGCCACCAACTCGGTGCTGTGGTTCTTCGGCGACGACATTCCGTTCGTG
CCGAACAAGCGTGCCGGCGGCTTCTGCTTCGGCACCAAGATCGCGCCGATCTTCTACAACACCATGGAAGAT
GCCGGCGCCCTGCCGATCGAGTTCGACTGCACCAACCTGGCCATGGGCGACGTCATCGACGTATACCCCTAT
GAAGGCAAGGTCGTTCGCCACGACAGCGGCGAAGTGGTCACCACCTTCGAGCTGAAGACCCCGGTCCTGCTC
GACGAAGTCCGCGCCGGCGGCCGTATCCCGCTGATCGTCGGCCGTGGCCTGACCGAGAAGGCCCGCGCCGAG
CTGGGCCTGGGCGCCTCCGACCTGTTCCGCAAGCCGGAAGCACCGGCCGATAGCGGCAAGGGCTTCACCCTG
GCGCAGAAAATGGTCGGCCGCGCCTGCGGTCTGCCGGAAGGCCAGGGCGTGCGCCCGGGCACCTACTGCGAA
CCGAAGATGACCACCGTCGGTTCCCAGGACACCACCGGCCCGATGACCCGCGACGAACTGAAGGACCTCGCC
TGCCTCGGCTTCTCCGCCGACCTGGTGATGCAGTCCTTCTGCCACACCGCGGCCTATCCGAAGCCGATCGAC
GTCAAGACCCACCACACCCTGCCCGACTTCATCATGACCCGTGGCGGCGTGTCGCTGCGCCCGGGCGACGGC
ATCATCCACAGCTGGCTGAACCGCATGCTGCTGCCCGATACCGTTGGTACCGGCGGCGACTCGCACACCCGC
TTCCCGATCGGCATCTCGTTCCCGGCCGGCTCCGGCCTGGTGGCCTTCGCCGCCGCCACCGGCGTGATGCCG
CTGGACATGCCGGAGTCGGTGCTGGTGCGCTTCAAGGGCAAGCTGCAACCGGGCATCACCCTGCGTGACCTG
GTCCATGCGATCCCTTACTACGCGATCCAGCAGGGCCTGCTGACCGTCGAGAAGAAAGGCAAGAAGAACATC
TTCTCCGGCCGCATCCTCGAGATCGAAGGCCTCAACGACCTGACCGTGGAACAGGCCTTCGAGCTGTCCGAC
GCCTCGGCCGAGCGTTCCGCCGCCGGCTGCACCATCAAGCTGCCGGAACAGGCGATCGCCGAGTACCTGAAG
TCCAACATCACCCTGCTGCGCTGGATGATCGGCGAAGGCTACGGCGACCCGCGCACCCTGGAGCGTCGCGCC
CAGGCGATGGAAGCCTGGCTGGCCAAGCCGGAGCTGCTGGAAGCCGACAAGGACGCCGAGTACGCCGCGGTG
ATCGAGATCGACCTGGCCGACGTCAAGGAGCCGGTGCTCTGCGCGCCGAACGATCCGGACGACGCGCGTCTG
CTGTCGAGCGTACAGGGCCGCAAGATCGATGAAGTGTTCATCGGCTCCTGCATGACCAACATCGGTCACTTC
CGCGCAGCCGGCAAGCTGCTGGACAAGGTCAAGGGCGGCATCCCGACCCGTCTGTGGCTGGCTCCGCCGACC
AAGATGGACGCCCACCAGTTGACCGAGGAAGGCTACTACGGCATCTACGGCAAGGCTGGCGCGCGCATGGAA
ATGCCGGGCTGCTCGCTGTGCATGGGCAACCAGGCTCGCGTCCAGACCGGTTCCACCGTGGTCTCCACCTCG
ACCCGTAACTTCCCGAACCGTCTGGGCGATGCCACCGATGTGTTCCTGGCATCGGCCGAACTGGCGGCGGTC
TCCTCGATCCTCGGCAAACTGCCGACCGTGGAGGAGTACATGGCCTACGCGAAGGACATCGACAGCATGGCT
GCCGACGTCTACCGCTACCTGAGCTTCGACCAGATCGCCGAGTTCCGCGAAGCGGCTGCGAACGCCAAGATC
CCGGTCGTTCAGGCCTGA
Translation:
MLEAYRKHVEERAAQGVVPQPLNAEQTAGLVELLKNPPAGEEEFLLDLITNRVPPGVDEAAYVKAGFLSAIV
KGEATSPLIDKQRAAELLGTMQGGYNIATLVELLDDAELANTAAEQLKHTLLMFDAFHDVAERAKKGNAAAK
SVLQSWADGEWFKAKPEVPEKLTLTVFKVPGETNTDDLSPAPDAWSRPDIPLHALAMLKMARDGIEPVQPGS
VGPLKQIEAVKAKGFPVAYVGDVVGTGSSRKSATNSVLWFFGDDIPFVPNKRAGGFCFGTKIAPIFYNTMED
AGALPIEFDCTNLAMGDVIDVYPYEGKVVRHDSGEVVTTFELKTPVLLDEVRAGGRIPLIVGRGLTEKARAE
LGLGASDLFRKPEAPADSGKGFTLAQKMVGRACGLPEGQGVRPGTYCEPKMTTVGSQDTTGPMTRDELKDLA
CLGFSADLVMQSFCHTAAYPKPIDVKTHHTLPDFIMTRGGVSLRPGDGIIHSWLNRMLLPDTVGTGGDSHTR
FPIGISFPAGSGLVAFAAATGVMPLDMPESVLVRFKGKLQPGITLRDLVHAIPYYAIQQGLLTVEKKGKKNI
FSGRILEIEGLNDLTVEQAFELSDASAERSAAGCTIKLPEQAIAEYLKSNITLLRWMIGEGYGDPRTLERRA
QAMEAWLAKPELLEADKDAEYAAVIEIDLADVKEPVLCAPNDPDDARLLSSVQGRKIDEVFIGSCMTNIGHF
RAAGKLLDKVKGGIPTRLWLAPPTKMDAHQLTEEGYYGIYGKAGARMEMPGCSLCMGNQARVQTGSTVVSTS
TRNFPNRLGDATDVFLASAELAAVSSILGKLPTVEEYMAYAKDIDSMAADVYRYLSFDQIAEFREAAANAKI
PVVQA*
AnnotationID:142GeneID:132
AnnotatorUID: diggins
Modification Date/Time: 2005-04-28 13:01:59
Gene Name:acnB
Confidence Code:2
GeneProduct:aconitate hydratase 2
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(12) Energy metabolism
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:COG1049,cd01581,pfam06434
Secondary Functional Category(ies):
EC Number:4.2.1.3
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAN67952.1|AE016427_7 (AE016782) aconitate hydratase 2 [Pseudomonas putida KT2440]
          Length = 869

 Score = 1617 bits (4186), Expect = 0.0
 Identities = 802/869 (92%), Positives = 832/869 (95%)

>gb|AAO57221.1| (AE016869) aconitate hydratase 2 [Pseudomonas syringae pv. tomato
           str. DC3000]
          Length = 866

 Score = 1537 bits (3980), Expect = 0.0
 Identities = 755/868 (86%), Positives = 811/868 (93%), Gaps = 3/868 (0%)

>gb|AAN78641.1|AE016755_141 (AE016755) Aconitate hydratase 2 [Escherichia coli CFT073]
          Length = 916

 Score = 1387 bits (3590), Expect = 0.0
 Identities = 680/859 (79%), Positives = 758/859 (88%), Gaps = 3/859 (0%)








 
Structural Features:
COG1049, AcnB, Aconitase B [Energy production and conversion]
CD-Length = 852 residues, 100.0% aligned
Score = 1526 bits (3951), Expect = 0.0
Identities = 699/864 (80%), Positives = 759/864 (87%), Gaps = 12/864 (1%)

cd01581, AcnB, Aconitase B catalytic domain. 
CD-Length = 436 residues, 100.0% aligned
Score =  756 bits (1954), Expect = 0.0

pfam06434, Aconitase_2_N, Aconitate hydratase 2 N-terminus.
CD-Length = 383 residues, 100.0% aligned
Score =  628 bits (1621), Expect = 0.0
Identities = 285/384 (74%), Positives = 321/384 (83%), Gaps = 4/384 (1%)






   
Genomic Context:






   
Comment:


















    ReferenceID:14061




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:17409




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:37810




  
Author/Investigator(s):
        Gruer MJ, Guest JR.





  
Title:
        Two genetically-distinct and differentially-regulated aconitases (AcnA and AcnB) in Escherichia coli.




  
PubMed:
        8000525


MedLine:
        




    
Source:
Microbiology. 1994 Oct;140 ( Pt 10):2531-41.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=95093601&dopt=Abstract

















    ReferenceID:37811




  
Author/Investigator(s):
        Bradbury AJ, Gruer MJ, Rudd KE, Guest JR.





  
Title:
        The second aconitase (AcnB) of Escherichia coli.




  
PubMed:
        8932712


MedLine:
        




    
Source:
Microbiology. 1996 Feb;142 ( Pt 2):389-400.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=97086518&dopt=Abstract

















    ReferenceID:38054




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:132_cdd.html


    		




 
URL:









           
Homologs By Global Alignment 

           

       



     
Gene ID:132
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
245
                           PA1787_tr
                           translation of PA1787
                           869
                           99.99
                           99.99
                           0
                           4487.0
     

     
     
17214
                           gb|AAG05176.1|AE004604_6
                           (AE004604) aconitate hydratase 2 [Pseudomonas aeruginosa PAO1]
                           869
                           99.88
                           99.99
                           0
                           4484.0
     

     
     
17215
                           gb|AAN67952.1|AE016427_7
                           (AE016782) aconitate hydratase 2 [Pseudomonas putida KT2440]
                           869
                           92.17
                           95.74
                           0
                           4182.0
     

     
     
17216
                           gb|AAO57221.1|
                           (AE016869) aconitate hydratase 2 [Pseudomonas syringae pv. tomato str. DC3000]
                           869
                           86.76
                           93.32
                           3
                           3979.0
     

     
     
17219
                           gb|AAC73229.1|
                           (AE000121) aconitate hydrase B [Escherichia coli K12]
                           871
                           78.18
                           87.25
                           8
                           3583.0
     

     






      

          
GC ORFID: 43134How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA1787Glimmer Score: 

          
Start: 1256879Stop: 1254270

          
Length: 2610


          
Start Codon: GTGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA1787 translation of PA1787Homolog Bit Score 1732.0

          
Other Homologs:  psyr_15may02_Scaffold1_revised_gene657 (ORF: BLASTX 1541.0), t_PA1787 (ORF: GLIMMER)

          
GC ORF Sequence

           
GTGCTTGAAGCCTACCGCAAACACGTAGAAGAGCGTGCCGCCCAGGGTGTCGTGCCCCAACCGCTGAACGCC 
GAACAGACCGCAGGCCTGGTCGAGCTGCTGAAGAACCCGCCGGCCGGCGAAGAAGAATTCCTCCTTGACCTG 
ATCACCAACCGCGTACCGCCGGGCGTCGACGAAGCCGCCTACGTCAAGGCCGGATTCCTTTCCGCCATCGTC 
AAGGGCGAAGCCACCTCCCCCCTTATCGACAAGCAACGTGCCGCCGAGCTGCTGGGCACCATGCAGGGCGGC 
TACAACATCGCCACCCTGGTCGAACTGCTGGACGACGCCGAACTGGCCAACACCGCCGCCGAACAGCTGAAG 
CACACCCTGCTGATGTTCGACGCCTTCCACGACGTCGCCGAGCGCGCCAAGAAGGGCAATGCCGCGGCCAAG 
TCCGTGCTGCAATCCTGGGCCGATGGCGAGTGGTTCAAGGCCAAGCCGGAAGTCCCGGAAAAGCTGACTCTC 
ACCGTATTCAAGGTCCCCGGCGAAACCAACACCGACGACCTCTCCCCGGCCCCCGACGCCTGGTCGCGCCCG 
GACATCCCGCTGCACGCCCTGGCCATGCTGAAGATGGCTCGCGACGGCATCGAGCCGGTCCAGCCGGGTTCG 
GTCGGTCCGCTGAAGCAGATCGAAGCGGTCAAGGCCAAGGGCTTCCCGGTCGCCTACGTCGGCGACGTGGTC 
GGCACCGGTTCCTCGCGCAAATCCGCCACCAACTCGGTGCTGTGGTTCTTCGGCGACGACATTCCGTTCGTG 
CCGAACAAGCGTGCCGGCGGCTTCTGCTTCGGCACCAAGATCGCGCCGATCTTCTACAACACCATGGAAGAT 
GCCGGCGCCCTGCCGATCGAGTTCGACTGCACCAACCTGGCCATGGGCGACGTCATCGACGTATACCCCTAT 
GAAGGCAAGGTCGTTCGCCACGACAGCGGCGAAGTGGTCACCACCTTCGAGCTGAAGACCCCGGTCCTGCTC 
GACGAAGTCCGCGCCGGCGGCCGTATCCCGCTGATCGTCGGCCGTGGCCTGACCGAGAAGGCCCGCGCCGAG 
CTGGGCCTGGGCGCCTCCGACCTGTTCCGCAAGCCGGAAGCACCGGCCGATAGCGGCAAGGGCTTCACCCTG 
GCGCAGAAAATGGTCGGCCGCGCCTGCGGTCTGCCGGAAGGCCAGGGCGTGCGCCCGGGCACCTACTGCGAA 
CCGAAGATGACCACCGTCGGTTCCCAGGACACCACCGGCCCGATGACCCGCGACGAACTGAAGGACCTCGCC 
TGCCTCGGCTTCTCCGCCGACCTGGTGATGCAGTCCTTCTGCCACACCGCGGCCTATCCGAAGCCGATCGAC 
GTCAAGACCCACCACACCCTGCCCGACTTCATCATGACCCGTGGCGGCGTGTCGCTGCGCCCGGGCGACGGC 
ATCATCCACAGCTGGCTGAACCGCATGCTGCTGCCCGATACCGTTGGTACCGGCGGCGACTCGCACACCCGC 
TTCCCGATCGGCATCTCGTTCCCGGCCGGCTCCGGCCTGGTGGCCTTCGCCGCCGCCACCGGCGTGATGCCG 
CTGGACATGCCGGAGTCGGTGCTGGTGCGCTTCAAGGGCAAGCTGCAACCGGGCATCACCCTGCGTGACCTG 
GTCCATGCGATCCCTTACTACGCGATCCAGCAGGGCCTGCTGACCGTCGAGAAGAAAGGCAAGAAGAACATC 
TTCTCCGGCCGCATCCTCGAGATCGAAGGCCTCAACGACCTGACCGTGGAACAGGCCTTCGAGCTGTCCGAC 
GCCTCGGCCGAGCGTTCCGCCGCCGGCTGCACCATCAAGCTGCCGGAACAGGCGATCGCCGAGTACCTGAAG 
TCCAACATCACCCTGCTGCGCTGGATGATCGGCGAAGGCTACGGCGACCCGCGCACCCTGGAGCGTCGCGCC 
CAGGCGATGGAAGCCTGGCTGGCCAAGCCGGAGCTGCTGGAAGCCGACAAGGACGCCGAGTACGCCGCGGTG 
ATCGAGATCGACCTGGCCGACGTCAAGGAGCCGGTGCTCTGCGCGCCGAACGATCCGGACGACGCGCGTCTG 
CTGTCGAGCGTACAGGGCCGCAAGATCGATGAAGTGTTCATCGGCTCCTGCATGACCAACATCGGTCACTTC 
CGCGCAGCCGGCAAGCTGCTGGACAAGGTCAAGGGCGGCATCCCGACCCGTCTGTGGCTGGCTCCGCCGACC 
AAGATGGACGCCCACCAGTTGACCGAGGAAGGCTACTACGGCATCTACGGCAAGGCTGGCGCGCGCATGGAA 
ATGCCGGGCTGCTCGCTGTGCATGGGCAACCAGGCTCGCGTCCAGACCGGTTCCACCGTGGTCTCCACCTCG 
ACCCGTAACTTCCCGAACCGTCTGGGCGATGCCACCGATGTGTTCCTGGCATCGGCCGAACTGGCGGCGGTC 
TCCTCGATCCTCGGCAAACTGCCGACCGTGGAGGAGTACATGGCCTACGCGAAGGACATCGACAGCATGGCT 
GCCGACGTCTACCGCTACCTGAGCTTCGACCAGATCGCCGAGTTCCGCGAAGCGGCTGCGAACGCCAAGATC 
CCGGTCGTTCAGGCCTGA