ViewGene
Locus Name:
GeneID:
Location:

GeneID:1317
TrivialName: PA14_48100
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_48100
PAO1 Ortholog Locus:PA1247
Sequence Length:1299
Protein Length:432
Start: 4282070
Stop: 4280772
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG04636.1|AE004554_4 (AE004554) alkaline protease secretion protein AprE [Pseudomonas aeruginosa PAO1]
Identities = 431/432 (99%), Positives = 431/432 (99%)
Sequence:
ATGACCCGCACGGTGAAACGCGACGAGAACGCCTATGCGCGACTGGGTTGGCTGCTGGTCCTGTTCGGCTTC
GGCGGCGCGCTGCTCTGGGCCGCCTTCGCGCCGCTCGACCAGGGCGTGGCGGTGCCGGCCACGGTGATCATC
TCCGGCCAGCGCAAGTCGGTGCAGCATCCGCTGGGCGGGGTGGTCAAGCACATCCTGGTGCGCGACGGCCAG
CATGTGGAGGCGGGCGAGCCGCTGATCCGCATGGAACCGACCCAGGCCCGGGCCAACGTCGATTCGCTGCTC
AACCGGTACGCCAACGCGCGGCTCAACCAGGCGCGCCTGCAGGCCGAATACGACGGCCGGCGGACCCTGGAG
ATGCCCGCGGGGCTGGCCGAGCAGGCCCCGCTGCCGACCCTCGGCGAGCGCCTGGAGTTGCAGCGGCAGTTG
CTGCACAGCCGCCAGACCGCGCTGGCCAACGAACTCTCCGCATTGCGGGCGAACATCGAGGGGCTGCGCGCC
CAGCTCGAAGGGTTGCGCCAGACCGAGGGCAACCAGCGCCTGCAACAACGCCTGTTGAACAGCCAGTTGAGC
GGTGCGCGCGACCTCGCCGAGGAAGGCTACATGCCGCGCAACCAGTTGCTCGAACAGGAGCGCCAACTGGCC
GAGGTGAACGCCCGGCTATCGGAGAGCAGCGGTCGCTTCGGGCAGATCCGCCAGAGCATCGCCGAGGCGCAG
ATGCGCATCGCCCAACGCGAGGAGGAGTACCGCAAGGAAGTCAACGGGCAACTGGCGGAAACCCAGGTCAAT
GCGCGGACGCTCTGGGAGGAGCTGTCCTCGGCGCGCTACGAACTGCGGCACGCCGAGATCCGCGCACCGGTC
AGCGGCTACGTGGCCGGCTTGAAGGTGTTCACCGATGGCGGGGTGATCGGCCCCGGCGAATTGCTGATGTAC
ATCGTGCCCAACAGCGACAGCCTGGAAGTGGAGGGCCAGCTCGCGGTGAACCTGGTCGACCGGATCCACAGC
GGGCTGCCGGTGGAGATGCTGTTCACCGCCTTCAACCAGAGCAAGACCCCGCGGGTCACCGGCGAGGTGACC
ATGGTCAGCGCCGACCGCCTGCTCGACGAGCAGAACAAGCAGCCCTACTACGCGCTGCGCGCCCAGGTCGAC
GCGGCCGGGATGGGCAAGCTGAAGGGCCTGCAGATCCGCCCGGGAATGGCGGTGCAGGTGTTCGTCCGCACC
GGCGAGCGCTCGCTGCTCAACTACCTGTTCAAGCCCCTGTTCGACCGCGCCCACGTGGCGCTGGCGGAGAAC
TGA
Translation:
MTRTVKRDENAYARLGWLLVLFGFGGALLWAAFAPLDQGVAVPATVIISGQRKSVQHPLGGVVKHILVRDGQ
HVEAGEPLIRMEPTQARANVDSLLNRYANARLNQARLQAEYDGRRTLEMPAGLAEQAPLPTLGERLELQRQL
LHSRQTALANELSALRANIEGLRAQLEGLRQTEGNQRLQQRLLNSQLSGARDLAEEGYMPRNQLLEQERQLA
EVNARLSESSGRFGQIRQSIAEAQMRIAQREEEYRKEVNGQLAETQVNARTLWEELSSARYELRHAEIRAPV
SGYVAGLKVFTDGGVIGPGELLMYIVPNSDSLEVEGQLAVNLVDRIHSGLPVEMLFTAFNQSKTPRVTGEVT
MVSADRLLDEQNKQPYYALRAQVDAAGMGKLKGLQIRPGMAVQVFVRTGERSLLNYLFKPLFDRAHVALAEN
*
AnnotationID:1326GeneID:1317
AnnotatorUID: diggins
Modification Date/Time: 2005-02-22 09:09:57
Gene Name:aprE
Confidence Code:1
GeneProduct:alkaline protease secretion protein AprE
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(23) Secreted Factors (toxins, enzymes, alginate)
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:pfam00529
Secondary Functional Category(ies):protein secretion/export apparatus
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>dbj|BAA88493.1| (AB023289) membrane fusion protein [Pseudomonas fluorescens]
          Length = 443

 Score =  430 bits (1106), Expect = e-120
 Identities = 220/424 (51%), Positives = 297/424 (70%), Gaps = 3/424 (0%)

>emb|CAD13437.1| (AJ421517) putative metalloprotease secretion protein [Yersinia
           ruckeri]
          Length = 443

 Score =  392 bits (1008), Expect = e-108
 Identities = 204/427 (47%), Positives = 286/427 (66%)

>gb|AAO56807.1| (AE016868) ABC transporter, periplasmic substrate-binding protein
           [Pseudomonas syringae pv. tomato str. DC3000]
          Length = 417

 Score =  381 bits (979), Expect = e-105
 Identities = 208/416 (50%), Positives = 277/416 (66%), Gaps = 4/416 (0%)






 
Structural Features:
pfam00529, HlyD, HlyD family secretion protein.
CD-Length = 309 residues, 100.0% aligned
Score =  171 bits (435), Expect = 1e-43
Identities = 109/317 (34%), Positives = 155/317 (48%), Gaps = 13/317 (4%)






   
Genomic Context:






   
Comment:


















    ReferenceID:2637




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:19780




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:37563




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:1317_cdd.html


    		




 
URL:

















    ReferenceID:38041




  
Author/Investigator(s):
        Duong F, Lazdunski A, Cami B, Murgier M.





  
Title:
        Sequence of a cluster of genes controlling synthesis and secretion of alkaline protease in Pseudomonas aeruginosa: relationships to other secretory pathways.




  
PubMed:
        1427098


MedLine:
        




    
Source:
Gene. 1992 Nov 2;121(1):47-54.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=1427098









           
Homologs By Global Alignment 

           

       



     
Gene ID:1317
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
2402
                           PA1247_tr
                           translation of PA1247
                           432
                           99.76
                           99.76
                           0
                           2156.0
     

     
     
76745
                           gb|AAG04636.1|AE004554_4
                           (AE004554) alkaline protease secretion protein AprE [Pseudomonas aeruginosa PAO1]
                           432
                           99.76
                           99.76
                           0
                           2156.0
     

     
     
76746
                           emb|CAA45856.1|
                           (X64558) aprE [Pseudomonas aeruginosa]
                           432
                           99.30
                           99.53
                           0
                           2148.0
     

     
     
2403
                           PA3405_tr
                           translation of PA3405
                           446
                           50.22
                           67.04
                           17
                           1098.0
     

     
     
76748
                           gb|AAG06793.1|AE004761_9
                           (AE004761) metalloprotease secretion protein [Pseudomonas aeruginosa PAO1]
                           446
                           50.22
                           67.04
                           17
                           1098.0
     

     






      

          
GC ORFID: 44319How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA1247Glimmer Score: 

          
Start: 671839Stop: 673137

          
Length: 1299


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA1247 translation of PA1247Homolog Bit Score 835.0

          
Other Homologs:  psyr_15may02_Scaffold17_revised_gene2828 (ORF: BLASTX 396.0), t_PA1247 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGACCCGCACGGTGAAACGCGACGAGAACGCCTATGCGCGACTGGGTTGGCTGCTGGTCCTGTTCGGCTTC 
GGCGGCGCGCTGCTCTGGGCCGCCTTCGCGCCGCTCGACCAGGGCGTGGCGGTGCCGGCCACGGTGATCATC 
TCCGGCCAGCGCAAGTCGGTGCAGCATCCGCTGGGCGGGGTGGTCAAGCACATCCTGGTGCGCGACGGCCAG 
CATGTGGAGGCGGGCGAGCCGCTGATCCGCATGGAACCGACCCAGGCCCGGGCCAACGTCGATTCGCTGCTC 
AACCGGTACGCCAACGCGCGGCTCAACCAGGCGCGCCTGCAGGCCGAATACGACGGCCGGCGGACCCTGGAG 
ATGCCCGCGGGGCTGGCCGAGCAGGCCCCGCTGCCGACCCTCGGCGAGCGCCTGGAGTTGCAGCGGCAGTTG 
CTGCACAGCCGCCAGACCGCGCTGGCCAACGAACTCTCCGCATTGCGGGCGAACATCGAGGGGCTGCGCGCC 
CAGCTCGAAGGGTTGCGCCAGACCGAGGGCAACCAGCGCCTGCAACAACGCCTGTTGAACAGCCAGTTGAGC 
GGTGCGCGCGACCTCGCCGAGGAAGGCTACATGCCGCGCAACCAGTTGCTCGAACAGGAGCGCCAACTGGCC 
GAGGTGAACGCCCGGCTATCGGAGAGCAGCGGTCGCTTCGGGCAGATCCGCCAGAGCATCGCCGAGGCGCAG 
ATGCGCATCGCCCAACGCGAGGAGGAGTACCGCAAGGAAGTCAACGGGCAACTGGCGGAAACCCAGGTCAAT 
GCGCGGACGCTCTGGGAGGAGCTGTCCTCGGCGCGCTACGAACTGCGGCACGCCGAGATCCGCGCACCGGTC 
AGCGGCTACGTGGCCGGCTTGAAGGTGTTCACCGATGGCGGGGTGATCGGCCCCGGCGAATTGCTGATGTAC 
ATCGTGCCCAACAGCGACAGCCTGGAAGTGGAGGGCCAGCTCGCGGTGAACCTGGTCGACCGGATCCACAGC 
GGGCTGCCGGTGGAGATGCTGTTCACCGCCTTCAACCAGAGCAAGACCCCGCGGGTCACCGGCGAGGTGACC 
ATGGTCAGCGCCGACCGCCTGCTCGACGAGCAGAACAAGCAGCCCTACTACGCGCTGCGCGCCCAGGTCGAC 
GCGGCCGGGATGGGCAAGCTGAAGGGCCTGCAGATCCGCCCGGGAATGGCGGTGCAGGTGTTCGTCCGCACC 
GGCGAGCGCTCGCTGCTCAACTACCTGTTCAAGCCCCTGTTCGACCGCGCCCACGTGGCGCTGGCGGAGAAC 
TGA