ViewGene
Locus Name:
GeneID:
Location:

GeneID:1316
TrivialName: PA14_63250
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_63250
PAO1 Ortholog Locus:PA4785
Sequence Length:1278
Protein Length:425
Start: 5645637
Stop: 5644360
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG08171.1|AE004892_2 (AE004892) probable acyl-CoA thiolase [Pseudomonas aeruginosa PAO1]
Identities = 423/425 (99%), Positives = 425/425 (100%)
Sequence:
ATGACCCAGCTGCGCCGGGTCGCCATTGTCGGCGGCAACCGTATTCCCTTCGCCCGCTCGAACACCGTGTAC
GCCACGGCGAGCAACCAGGAGATGCTCACTTCCGCCCTGGAAGGCCTGGTGGAGCGCTACAACCTGCATGGC
GAGCGTCTTGGCGAGGTGGTGGCCGGCGCCGTGCTGAAGCATTCGCGCGACTTCAACCTGACCCGCGAATGC
GTGCTCGGTTCGCGCCTGGCCCCGGAAACCCCGGCCTACGATATCCAGCAGGCCTGCGGTACCGGCCTGGAG
GCGGCGATCCTGGTGGCCAACAAGATCGCCCTCGGGCAGATCGACAGCGGCATCGCCGGCGGCGTCGACACC
ACTTCCGACGCGCCGATCGGGGTCAACGAGGGTCTGCGCAAGATTCTCCTCGAGGCCAACCGTGGCAAGAGC
AACCTGGACAAGCTCAGGAGCCTATTGAAGATCCGCCCGCGCCACGTGGTCCCGGCCATCCCGCGCAACGGC
GAGCCGCGCACCGGGCTGTCGATGGGGGAGTCCTGCGAACTGATGGCGCAGACCTGGCAGATTCCCCGCGAC
GAGCAGGACAGGCTGGCCTTCGAGAGCCACCACAAGCTGGCCGCGGCCTATGAGGAAGGCTGGCAGAACGAC
CTGATGACGCCGTTCCGCGGCCTGACCCGCGACCAGAACCTGCGCCCGGATATCGACCTGGAGAAGATCGGC
ACCCTCAAGCCGGTGTTCGAGCGCGGCTCGCGCGGCACCCTCACGGCGGCCAACTCGACGCCGCTGACCGAC
GGCGCCTCGGTGGTACTGCTGGCCAGCGAGGAGTGGGCGAAGGCGCGCGGCCTGCCGATCCTGGCCTACTTC
AAGGACGGCGAGGCGGCGGCGGTGAACTTCGTCGATCGCCAGGAGGGCCTGCTGATGGCGCCGGTCTACGCG
GTGCCGCGGCTGCTGGCACGCAACGGCCTGAGCCTGCAGGACTTCGACTACTACGAGATCCACGAGGCCTTC
GCCGCCCAGGTGCTCTGCACCCTGAAGGCCTGGGAAGACCCCGAGTACTGCAAGACCCGTCTCGGCCTCGAC
CAGCCGCTGGGCTCCATCGACCGCAGCAAGCTCAACGTCAAGGGCAGCTCGCTGGCCGCCGGCCACCCGTTC
GCCGCCACGGGAGGACGGATCGTCGCCAACCTGGCCAAGCTGCTCTCGGTGGCGGAGCAGGGACGCGGGCTG
ATTTCCATCTGCGCCGCCGGTGGGCAGGGTGTGACCGCGATCATCGAGCGGTAA
Translation:
MTQLRRVAIVGGNRIPFARSNTVYATASNQEMLTSALEGLVERYNLHGERLGEVVAGAVLKHSRDFNLTREC
VLGSRLAPETPAYDIQQACGTGLEAAILVANKIALGQIDSGIAGGVDTTSDAPIGVNEGLRKILLEANRGKS
NLDKLRSLLKIRPRHVVPAIPRNGEPRTGLSMGESCELMAQTWQIPRDEQDRLAFESHHKLAAAYEEGWQND
LMTPFRGLTRDQNLRPDIDLEKIGTLKPVFERGSRGTLTAANSTPLTDGASVVLLASEEWAKARGLPILAYF
KDGEAAAVNFVDRQEGLLMAPVYAVPRLLARNGLSLQDFDYYEIHEAFAAQVLCTLKAWEDPEYCKTRLGLD
QPLGSIDRSKLNVKGSSLAAGHPFAATGGRIVANLAKLLSVAEQGRGLISICAAGGQGVTAIIER*
AnnotationID:1325GeneID:1316
AnnotatorUID: diggins
Modification Date/Time: 2005-03-10 08:08:39
Gene Name:
Confidence Code:3
GeneProduct:putative acyl-CoA thiolase
Cell Localization:()
Synonyms:yfcY
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(13) Fatty acid and phospholipid metabolism
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:cd00751,COG0183,pfam00108
Secondary Functional Category(ies):central intermediary metabolism
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAO54286.1| (AE016858) thiolase family protein [Pseudomonas syringae pv. tomato
           str. DC3000]
          Length = 425

 Score =  691 bits (1784), Expect = 0.0
 Identities = 342/425 (80%), Positives = 382/425 (89%)

>gb|AAN66209.1|AE016249_1 (AE016776) thiolase family protein [Pseudomonas putida KT2440]
          Length = 425

 Score =  665 bits (1715), Expect = 0.0
 Identities = 330/425 (77%), Positives = 369/425 (86%)

>emb|CAC32095.1| (AL583926) acetyl-CoA C-acetyltransferase [Mycobacterium leprae]
          Length = 457

 Score =  491 bits (1263), Expect = e-138
 Identities = 257/428 (60%), Positives = 317/428 (74%), Gaps = 9/428 (2%)








 
Structural Features:
cd00751, thiolase, Thiolase are ubiquitous enzymes that catalyze the 
reversible thiolytic cleavage of 3-ketoacyl-CoA into acyl-CoA and acetyl-CoA
CD-Length = 386 residues, 100.0% aligned
Score =  362 bits (932), Expect = 3e-101

COG0183, PaaJ, Acetyl-CoA acetyltransferase [Lipid metabolism]
CD-Length = 392 residues,  99.5% aligned
Score =  220 bits (562), Expect = 2e-58
Identities = 134/437 (30%), Positives = 194/437 (44%), Gaps = 63/437 (14%)

pfam00108, Thiolase_N, Thiolase, N-terminal domain. 
CD-Length = 264 residues, 100.0% aligned
Score =  160 bits (406), Expect = 3e-40
Identities = 85/283 (30%), Positives = 131/283 (46%), Gaps = 30/283 (10%)






   
Genomic Context:






   
Comment:


















    ReferenceID:2635




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:19778




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:37700




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:1316_cdd.html


    		




 
URL:









           
Homologs By Global Alignment 

           

       



     
Gene ID:1316
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
2401
                           PA4785_tr
                           translation of PA4785
                           425
                           99.52
                           99.99
                           0
                           2159.0
     

     
     
76730
                           gb|AAG08171.1|AE004892_2
                           (AE004892) probable acyl-CoA thiolase [Pseudomonas aeruginosa PAO1]
                           425
                           99.52
                           99.99
                           0
                           2159.0
     

     
     
76731
                           gb|AAO54286.1|
                           (AE016858) thiolase family protein [Pseudomonas syringae pv. tomato str. DC3000]
                           425
                           80.47
                           89.88
                           0
                           1784.0
     

     
     
76732
                           gb|AAN66209.1|AE016249_1
                           (AE016776) thiolase family protein [Pseudomonas putida KT2440]
                           425
                           77.64
                           86.82
                           0
                           1715.0
     

     
     
76733
                           dbj|BAA89375.1|
                           (AB025342) ORF1 [Moritella marina]
                           427
                           66.51
                           79.62
                           3
                           1458.0
     

     






      

          
GC ORFID: 44318How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA4785Glimmer Score: 

          
Start: 4909317Stop: 4908040

          
Length: 1278


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TAATruncated Stop: 

          
Homolog: t_PA4785 translation of PA4785Homolog Bit Score 836.0

          
Other Homologs:  psyr_15may02_Scaffold2_revised_gene3480 (ORF: BLASTX 693.0), t_PA4785 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGACCCAGCTGCGCCGGGTCGCCATTGTCGGCGGCAACCGTATTCCCTTCGCCCGCTCGAACACCGTGTAC 
GCCACGGCGAGCAACCAGGAGATGCTCACTTCCGCCCTGGAAGGCCTGGTGGAGCGCTACAACCTGCATGGC 
GAGCGTCTTGGCGAGGTGGTGGCCGGCGCCGTGCTGAAGCATTCGCGCGACTTCAACCTGACCCGCGAATGC 
GTGCTCGGTTCGCGCCTGGCCCCGGAAACCCCGGCCTACGATATCCAGCAGGCCTGCGGTACCGGCCTGGAG 
GCGGCGATCCTGGTGGCCAACAAGATCGCCCTCGGGCAGATCGACAGCGGCATCGCCGGCGGCGTCGACACC 
ACTTCCGACGCGCCGATCGGGGTCAACGAGGGTCTGCGCAAGATTCTCCTCGAGGCCAACCGTGGCAAGAGC 
AACCTGGACAAGCTCAGGAGCCTATTGAAGATCCGCCCGCGCCACGTGGTCCCGGCCATCCCGCGCAACGGC 
GAGCCGCGCACCGGGCTGTCGATGGGGGAGTCCTGCGAACTGATGGCGCAGACCTGGCAGATTCCCCGCGAC 
GAGCAGGACAGGCTGGCCTTCGAGAGCCACCACAAGCTGGCCGCGGCCTATGAGGAAGGCTGGCAGAACGAC 
CTGATGACGCCGTTCCGCGGCCTGACCCGCGACCAGAACCTGCGCCCGGATATCGACCTGGAGAAGATCGGC 
ACCCTCAAGCCGGTGTTCGAGCGCGGCTCGCGCGGCACCCTCACGGCGGCCAACTCGACGCCGCTGACCGAC 
GGCGCCTCGGTGGTACTGCTGGCCAGCGAGGAGTGGGCGAAGGCGCGCGGCCTGCCGATCCTGGCCTACTTC 
AAGGACGGCGAGGCGGCGGCGGTGAACTTCGTCGATCGCCAGGAGGGCCTGCTGATGGCGCCGGTCTACGCG 
GTGCCGCGGCTGCTGGCACGCAACGGCCTGAGCCTGCAGGACTTCGACTACTACGAGATCCACGAGGCCTTC 
GCCGCCCAGGTGCTCTGCACCCTGAAGGCCTGGGAAGACCCCGAGTACTGCAAGACCCGTCTCGGCCTCGAC 
CAGCCGCTGGGCTCCATCGACCGCAGCAAGCTCAACGTCAAGGGCAGCTCGCTGGCCGCCGGCCACCCGTTC 
GCCGCCACGGGAGGACGGATCGTCGCCAACCTGGCCAAGCTGCTCTCGGTGGCGGAGCAGGGACGCGGGCTG 
ATTTCCATCTGCGCCGCCGGTGGGCAGGGTGTGACCGCGATCATCGAGCGGTAA