ViewGene
Locus Name:
GeneID:
Location:

GeneID:1308
TrivialName: PA14_58320
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_58320
PAO1 Ortholog Locus:PA4494
Sequence Length:1269
Protein Length:422
Start: 5193829
Stop: 5192561
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG07882.1|AE004863_2 (AE004863) probable two-component sensor [Pseudomonas aeruginosa PAO1]
Identities = 422/422 (100%), Positives = 422/422 (100%)
Sequence:
ATGCGCGCACCGGTTTTCCTGCTCTCCGCCAGTCGCCAGAACCTTGGCCGTCTGCTCCTCATCCGTATTCTC
GTGCTGGCCGCGCAGGCCGGCTCGGTGGGTTTCGCCTGGCTCTCCGACCTGTTGCCGCTGCCGTGGCTGGCG
CTCGGCCTGACCCTTGGCCTGTCCGCCTGCCTGATTCTGTTCACCGCGCTGCGCCTGCGCGCGCCGTGGCCG
GTGACCGACCAGGAGTACGCGGTACAACTGGCCTGCGACCTGATCATCCACAGCACCTTGCTGTATTACTCG
GGCGGCTCGACCAACCCCTTCGTTTCCTATTACCTGGTACCGCTGACCATCGCCGCTGCGACCCTGCCCTGG
ATCTACTCCATGGTGCTCTCCGGCCTGGCCCTGACCGGCTACACCCTGCTGCTGATCTGGTACCACCCGCTG
CACCTGCAGGCGGTTCAGCACGAGGCGATGCTGATCAACCTGCACCTGTTCGGCATGTGGCTGAACTTCGCC
CTGTCGGCGGCCTTCATCACCTTCTTCGTGGTGAAGATGTCCGAGGCCCTGCGCTGGCAGGCGCACATGCAG
GCCGAGCGGCGCGAGGAAAGCATGCGCGACCAGCAGTTGCTCGCGGTGGCCACCCAGGCGGCCGGTGCTGCG
CACGAACTGGGTACGCCGCTGGCGACCATGAGCGTGCTGCTCAACGAGTTGCGCCAGGAGCGCCCGCAGGAC
CCGACCCTGCAGGAAGACCTGGCATTGCTCCAGGAACAGGTCAAGCAGTGCAAGCACACCCTCCAGCAACTG
GTACGGGCGGCCGAGGCCGACCGTCGCCAGGCGATCGAGGAGCAAAGCGCCGCGGAATGGCTGGAATCGGTG
CTGCGCCGCTGGCACCTGATGCGTCCGGAGGCTTCCTACCGCTACCAGTGCCTGGGCCTCGGCTCCGCGCCG
CGCCTGACCCCGCCGACCGACCTCAGCCAGGCTCTGCTGAACCTGCTGAACAATGCGGCGGACGCCTGTCCC
GACGATCTCGACATCCGCCTGGACTGGGACAGCAGCAGTATCTGCCTGAGTATCCGCGACCACGGCGTCGGG
GTGCCCCTGGCGATCGCCGAACAGATCGGCAAACCGTTCTTCACCACCAAGGGCAAGGGCTTCGGCCTCGGC
CTGTTTCTCAGTCAGGCCAGCGTGACCCGCGCTGGTGGAACGGTGAAGTTGTATAACCACGAAGATGGCGGC
ACTCTGACCGAACTGCGCTTGCCGCGCAGCTACGGAATGGCTTGA
Translation:
MRAPVFLLSASRQNLGRLLLIRILVLAAQAGSVGFAWLSDLLPLPWLALGLTLGLSACLILFTALRLRAPWP
VTDQEYAVQLACDLIIHSTLLYYSGGSTNPFVSYYLVPLTIAAATLPWIYSMVLSGLALTGYTLLLIWYHPL
HLQAVQHEAMLINLHLFGMWLNFALSAAFITFFVVKMSEALRWQAHMQAERREESMRDQQLLAVATQAAGAA
HELGTPLATMSVLLNELRQERPQDPTLQEDLALLQEQVKQCKHTLQQLVRAAEADRRQAIEEQSAAEWLESV
LRRWHLMRPEASYRYQCLGLGSAPRLTPPTDLSQALLNLLNNAADACPDDLDIRLDWDSSSICLSIRDHGVG
VPLAIAEQIGKPFFTTKGKGFGLGLFLSQASVTRAGGTVKLYNHEDGGTLTELRLPRSYGMA*
AnnotationID:1317GeneID:1308
AnnotatorUID: diggins
Modification Date/Time: 2005-05-04 06:06:55
Gene Name:
Confidence Code:3
GeneProduct:putative two-component sensor
Cell Localization:()
Synonyms:PA4494
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(28) Two-component regulatory systems
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:COG0642,smart00387,pfam02518,cd00075
Secondary Functional Category(ies):
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAO58002.1| (AE016872) sensor histidine kinase [Pseudomonas syringae pv. tomato
           str. DC3000]
          Length = 419

 Score =  591 bits (1523), Expect = e-168
 Identities = 298/417 (71%), Positives = 342/417 (82%), Gaps = 5/417 (1%)

>gb|AAN66512.1|AE016280_4 (AE016777) sensor histidine kinase [Pseudomonas putida KT2440]
          Length = 450

 Score =  573 bits (1478), Expect = e-163
 Identities = 293/416 (70%), Positives = 341/416 (81%), Gaps = 5/416 (1%)

>emb|CAD85225.1| (BX321860) probable two-component sensor [Nitrosomonas europaea
           ATCC 19718]
          Length = 462

 Score =  320 bits (821), Expect = 8e-87
 Identities = 173/417 (41%), Positives = 252/417 (60%), Gaps = 5/417 (1%)







 
Structural Features:
COG0642, BaeS, Signal transduction histidine kinase 
[Signal transduction mechanisms]
CD-Length = 336 residues,  96.7% aligned
Score =  114 bits (284), Expect = 5e-26
Identities = 79/327 (24%), Positives = 130/327 (39%), Gaps = 11/327 (3%)

smart00387, HATPase_c, Histidine kinase-like ATPases
CD-Length = 112 residues,  95.5% aligned
Score = 86.9 bits (215), Expect = 8e-18
Identities = 34/107 (31%), Positives = 55/107 (51%), Gaps = 7/107 (6%)

pfam02518, HATPase_c, Histidine kinase-, DNA gyrase B-, 
and HSP90-like ATPase.
CD-Length = 111 residues,  96.4% aligned
Score = 83.9 bits (207), Expect = 7e-17







   
Genomic Context:






   
Comment:


















    ReferenceID:2619




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:19761




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:31537




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:1308_cdd.html


    		




 
URL:









           
Homologs By Global Alignment 

           

       



     
Gene ID:1308
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
2371
                           PA4494_tr
                           translation of PA4494
                           422
                           99.99
                           99.99
                           0
                           2166.0
     

     
     
76215
                           gb|AAG07882.1|AE004863_2
                           (AE004863) probable two-component sensor [Pseudomonas aeruginosa PAO1]
                           422
                           99.99
                           99.99
                           0
                           2166.0
     

     
     
76216
                           gb|AAO58002.1|
                           (AE016872) sensor histidine kinase [Pseudomonas syringae pv. tomato str. DC3000]
                           424
                           70.51
                           81.13
                           7
                           1520.0
     

     
     
76217
                           gb|AAN66512.1|AE016280_4
                           (AE016777) sensor histidine kinase [Pseudomonas putida KT2440]
                           457
                           64.55
                           75.05
                           42
                           1484.0
     

     
     
76218
                           emb|CAD85225.1|
                           (BX321860) probable two-component sensor [Nitrosomonas europaea ATCC 19718]
                           472
                           37.07
                           54.66
                           60
                           819.0
     

     






      

          
GC ORFID: 44310How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA4494Glimmer Score: 

          
Start: 4457508Stop: 4456240

          
Length: 1269


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA4494 translation of PA4494Homolog Bit Score 838.0

          
Other Homologs:  psyr_15may02_Scaffold4_revised_gene5438 (ORF: BLASTX 596.0), t_PA4494 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGCGCGCACCGGTTTTCCTGCTCTCCGCCAGTCGCCAGAACCTTGGCCGTCTGCTCCTCATCCGTATTCTC 
GTGCTGGCCGCGCAGGCCGGCTCGGTGGGTTTCGCCTGGCTCTCCGACCTGTTGCCGCTGCCGTGGCTGGCG 
CTCGGCCTGACCCTTGGCCTGTCCGCCTGCCTGATTCTGTTCACCGCGCTGCGCCTGCGCGCGCCGTGGCCG 
GTGACCGACCAGGAGTACGCGGTACAACTGGCCTGCGACCTGATCATCCACAGCACCTTGCTGTATTACTCG 
GGCGGCTCGACCAACCCCTTCGTTTCCTATTACCTGGTACCGCTGACCATCGCCGCTGCGACCCTGCCCTGG 
ATCTACTCCATGGTGCTCTCCGGCCTGGCCCTGACCGGCTACACCCTGCTGCTGATCTGGTACCACCCGCTG 
CACCTGCAGGCGGTTCAGCACGAGGCGATGCTGATCAACCTGCACCTGTTCGGCATGTGGCTGAACTTCGCC 
CTGTCGGCGGCCTTCATCACCTTCTTCGTGGTGAAGATGTCCGAGGCCCTGCGCTGGCAGGCGCACATGCAG 
GCCGAGCGGCGCGAGGAAAGCATGCGCGACCAGCAGTTGCTCGCGGTGGCCACCCAGGCGGCCGGTGCTGCG 
CACGAACTGGGTACGCCGCTGGCGACCATGAGCGTGCTGCTCAACGAGTTGCGCCAGGAGCGCCCGCAGGAC 
CCGACCCTGCAGGAAGACCTGGCATTGCTCCAGGAACAGGTCAAGCAGTGCAAGCACACCCTCCAGCAACTG 
GTACGGGCGGCCGAGGCCGACCGTCGCCAGGCGATCGAGGAGCAAAGCGCCGCGGAATGGCTGGAATCGGTG 
CTGCGCCGCTGGCACCTGATGCGTCCGGAGGCTTCCTACCGCTACCAGTGCCTGGGCCTCGGCTCCGCGCCG 
CGCCTGACCCCGCCGACCGACCTCAGCCAGGCTCTGCTGAACCTGCTGAACAATGCGGCGGACGCCTGTCCC 
GACGATCTCGACATCCGCCTGGACTGGGACAGCAGCAGTATCTGCCTGAGTATCCGCGACCACGGCGTCGGG 
GTGCCCCTGGCGATCGCCGAACAGATCGGCAAACCGTTCTTCACCACCAAGGGCAAGGGCTTCGGCCTCGGC 
CTGTTTCTCAGTCAGGCCAGCGTGACCCGCGCTGGTGGAACGGTGAAGTTGTATAACCACGAAGATGGCGGC 
ACTCTGACCGAACTGCGCTTGCCGCGCAGCTACGGAATGGCTTGA