ViewGene
Locus Name:
GeneID:
Location:

GeneID:1307
TrivialName: PA14_17320
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_17320
PAO1 Ortholog Locus:PA3635
Sequence Length:1290
Protein Length:429
Start: 1488044
Stop: 1489333
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG07023.1|AE004783_8 (AE004783) enolase [Pseudomonas aeruginosa PAO1]
Identities = 429/429 (100%), Positives = 429/429 (100%)
Sequence:
ATGGCAAAGATCGTCGACATCAAGGGCCGTGAGGTCCTGGACTCCCGCGGCAACCCTACCGTTGAAGCGGAC
GTGATCCTGGACAACGGCATCGTCGGCAGCGCCTGCGCGCCTTCCGGTGCTTCCACCGGTTCCCGCGAGGCC
CTCGAGCTGCGCGATGGCGACAAGAGCCGTTACCTGGGCAAGGGCGTGCTGAAAGCCGTGGCCAACATCAAC
GGCCCGATCCGCGACCTGCTGCTGGGCAAGGACGCGGCCGACCAGAAAGCCCTCGACCACGCGATGATCGAG
CTGGACGGCACCGAGAACAAGGCCAAGCTGGGCGCCAACGCGATCCTCGCGGTGTCCCTGGCTGCCGCCAAG
GCCGCCGCACAGGCCAAGGGCGTACCGCTGTACGCGCACATCGCCGACCTCAACGGCACCCCCGGCCAGTAC
TCCATGCCGGTGCCGATGATGAACATCATCAACGGCGGCGAGCATGCGGACAACAACGTCGATATCCAGGAA
TTCATGGTCCAGCCGGTCGGCGCGAAGAACTTCGCCGAGGCGCTGCGCATGGGCGCCGAGATCTTCCATCAC
CTCAAGGCCGTGCTGAAGGCCCGTGGCCTGAACACCGCCGTCGGTGACGAAGGCGGCTTCGCGCCGAACCTG
TCGTCCAACGAAGACGCCCTGGCCGCCATCGCCGAGGCCGTCGAGAAGGCCGGCTACAAGCTGGGTGACGAC
GTGACCCTGGCCCTGGACTGCGCCTCCAGCGAATTCTTCAAGGACGGCAAGTACGACCTGGAAGGCGAAGGC
AAGGTATTCGACGCCGCCGGTTTCGCCGATTACCTGGCCGGCCTGACCCAGCGCTACCCGATCATCTCCATC
GAGGACGGCATGGACGAGTCCGACTGGGCCGGCTGGAAAGGCCTGACCGACAAGATCGGCGCCAAGGTCCAA
CTGGTCGGCGACGACCTGTTCGTGACCAACACCAAGATCCTCAAGGAAGGCATCGAGAAGGGCATCGGCAAT
TCGATCCTGATCAAGTTCAACCAGATCGGTTCGCTCACCGAGACCCTGGAGGCCATCCAGATGGCCAAGGCC
GCCGGCTATACCGCGGTGATCTCGCACCGCTCCGGCGAAACCGAGGACTCGACCATCGCCGACCTGGCCGTG
GGTACCGCCGCCGGTCAGATCAAGACCGGTTCGCTGTGCCGCTCCGACCGCGTGTCCAAGTACAACCAGCTG
CTGCGCATCGAAGAGCAGCTGGGCGCCAAGGCGCCGTACCGTGGTCGCGCGGAATTCCGCGGCTGA
Translation:
MAKIVDIKGREVLDSRGNPTVEADVILDNGIVGSACAPSGASTGSREALELRDGDKSRYLGKGVLKAVANIN
GPIRDLLLGKDAADQKALDHAMIELDGTENKAKLGANAILAVSLAAAKAAAQAKGVPLYAHIADLNGTPGQY
SMPVPMMNIINGGEHADNNVDIQEFMVQPVGAKNFAEALRMGAEIFHHLKAVLKARGLNTAVGDEGGFAPNL
SSNEDALAAIAEAVEKAGYKLGDDVTLALDCASSEFFKDGKYDLEGEGKVFDAAGFADYLAGLTQRYPIISI
EDGMDESDWAGWKGLTDKIGAKVQLVGDDLFVTNTKILKEGIEKGIGNSILIKFNQIGSLTETLEAIQMAKA
AGYTAVISHRSGETEDSTIADLAVGTAAGQIKTGSLCRSDRVSKYNQLLRIEEQLGAKAPYRGRAEFRG*
AnnotationID:1316GeneID:1307
AnnotatorUID: diggins
Modification Date/Time: 2005-04-20 20:08:22
Gene Name:eno
Confidence Code:1
GeneProduct:enolase
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(12) Energy metabolism
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:COG0148,KOG2670,pfam00113
Secondary Functional Category(ies):
EC Number:4.2.1.11
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAN67233.1|AE016350_6 (AE016780) enolase [Pseudomonas putida KT2440]
          Length = 429

 Score =  781 bits (2016), Expect = 0.0
 Identities = 397/429 (92%), Positives = 413/429 (96%)

>gb|AAN81792.1|AE016765_194 (AE016765) Enolase [Escherichia coli CFT073]
          Length = 432

 Score =  651 bits (1680), Expect = 0.0
 Identities = 329/430 (76%), Positives = 372/430 (86%), Gaps = 1/430 (0%)








 
Structural Features:
COG0148, Eno, Enolase [Carbohydrate transport and metabolism]
CD-Length = 423 residues,  99.8% aligned
Score =  641 bits (1654), Expect = 0.0
Identities = 283/429 (65%), Positives = 338/429 (78%), Gaps = 7/429 (1%)

KOG2670, Enolase [Carbohydrate transport and metabolism]
CD-Length = 433 residues,  99.1% aligned
Score =  501 bits (1291), Expect = 1e-142
Identities = 241/435 (55%), Positives = 308/435 (70%), Gaps = 16/435 (3%)

pfam00113, Enolase_C, Enolase, C-terminal TIM barrel domain.
CD-Length = 295 residues,  99.7% aligned
Score =  412 bits (1061), Expect = 7e-116
Identities = 164/296 (55%), Positives = 213/296 (71%), Gaps = 12/296 (4%)






   
Genomic Context:






   
Comment:


















    ReferenceID:2617




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:19759




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:31908




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:1307_cdd.html


    		




 
URL:

















    ReferenceID:34187




  
Author/Investigator(s):
        Walsh AG, Burrows LL, Lam JS.





  
Title:
        Genetic and biochemical characterization of an operon involved in the biosynthesis of 3-deoxy-D-manno-octulosonic acid in Pseudomonas aeruginosa.




  
PubMed:
        10220877


MedLine:
        




    
Source:
FEMS Microbiol Lett. 1999 Apr 1;173(1):27-33.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=10220877

















    ReferenceID:34188




  
Author/Investigator(s):
        Stamm LV, Young NR.





  
Title:
        Nucleotide sequence of the Treponema pallidum eno gene.




  
PubMed:
        9255517


MedLine:
        




    
Source:
DNA Seq. 1997;7(5):261-5.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=9255517

















    ReferenceID:34189




  
Author/Investigator(s):
        Klein M, Sprenger GA, Freudl R.





  
Title:
        Cloning, nucleotide sequence, and functional expression of the Escherichia coli enolase (eno) gene in a temperature-sensitive eno mutant strain.




  
PubMed:
        8988374


MedLine:
        




    
Source:
DNA Seq. 1996;6(6):351-5.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=8988374









           
Homologs By Global Alignment 

           

       



     
Gene ID:1307
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
2370
                           PA3635_tr
                           translation of PA3635
                           429
                           99.99
                           99.99
                           0
                           2164.0
     

     
     
76040
                           gb|AAG07023.1|AE004783_8
                           (AE004783) enolase [Pseudomonas aeruginosa PAO1]
                           429
                           99.99
                           99.99
                           0
                           2164.0
     

     
     
76041
                           gb|AAN67233.1|AE016350_6
                           (AE016780) enolase [Pseudomonas putida KT2440]
                           429
                           92.54
                           96.27
                           0
                           2016.0
     

     
     
76042
                           gb|AAO55074.1|
                           (AE016861) enolase [Pseudomonas syringae pv. tomato str. DC3000]
                           429
                           86.71
                           93.47
                           1
                           1890.0
     

     
     
76048
                           dbj|BAB37062.1|
                           (AP002562) enolase [Escherichia coli O157:H7]
                           432
                           76.15
                           86.11
                           3
                           1682.0
     

     






      

          
GC ORFID: 44309How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA3635Glimmer Score: 

          
Start: 3465893Stop: 3464604

          
Length: 1290


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA3635 translation of PA3635Homolog Bit Score 838.0

          
Other Homologs:  psyr_15may02_Scaffold3_revised_gene4641 (ORF: BLASTX 733.0), psyr_15may02_Scaffold10_revised_gene1610 (ORF: BLASTX 448.0), t_PA3635 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGGCAAAGATCGTCGACATCAAGGGCCGTGAGGTCCTGGACTCCCGCGGCAACCCTACCGTTGAAGCGGAC 
GTGATCCTGGACAACGGCATCGTCGGCAGCGCCTGCGCGCCTTCCGGTGCTTCCACCGGTTCCCGCGAGGCC 
CTCGAGCTGCGCGATGGCGACAAGAGCCGTTACCTGGGCAAGGGCGTGCTGAAAGCCGTGGCCAACATCAAC 
GGCCCGATCCGCGACCTGCTGCTGGGCAAGGACGCGGCCGACCAGAAAGCCCTCGACCACGCGATGATCGAG 
CTGGACGGCACCGAGAACAAGGCCAAGCTGGGCGCCAACGCGATCCTCGCGGTGTCCCTGGCTGCCGCCAAG 
GCCGCCGCACAGGCCAAGGGCGTACCGCTGTACGCGCACATCGCCGACCTCAACGGCACCCCCGGCCAGTAC 
TCCATGCCGGTGCCGATGATGAACATCATCAACGGCGGCGAGCATGCGGACAACAACGTCGATATCCAGGAA 
TTCATGGTCCAGCCGGTCGGCGCGAAGAACTTCGCCGAGGCGCTGCGCATGGGCGCCGAGATCTTCCATCAC 
CTCAAGGCCGTGCTGAAGGCCCGTGGCCTGAACACCGCCGTCGGTGACGAAGGCGGCTTCGCGCCGAACCTG 
TCGTCCAACGAAGACGCCCTGGCCGCCATCGCCGAGGCCGTCGAGAAGGCCGGCTACAAGCTGGGTGACGAC 
GTGACCCTGGCCCTGGACTGCGCCTCCAGCGAATTCTTCAAGGACGGCAAGTACGACCTGGAAGGCGAAGGC 
AAGGTATTCGACGCCGCCGGTTTCGCCGATTACCTGGCCGGCCTGACCCAGCGCTACCCGATCATCTCCATC 
GAGGACGGCATGGACGAGTCCGACTGGGCCGGCTGGAAAGGCCTGACCGACAAGATCGGCGCCAAGGTCCAA 
CTGGTCGGCGACGACCTGTTCGTGACCAACACCAAGATCCTCAAGGAAGGCATCGAGAAGGGCATCGGCAAT 
TCGATCCTGATCAAGTTCAACCAGATCGGTTCGCTCACCGAGACCCTGGAGGCCATCCAGATGGCCAAGGCC 
GCCGGCTATACCGCGGTGATCTCGCACCGCTCCGGCGAAACCGAGGACTCGACCATCGCCGACCTGGCCGTG 
GGTACCGCCGCCGGTCAGATCAAGACCGGTTCGCTGTGCCGCTCCGACCGCGTGTCCAAGTACAACCAGCTG 
CTGCGCATCGAAGAGCAGCTGGGCGCCAAGGCGCCGTACCGTGGTCGCGCGGAATTCCGCGGCTGA