ViewGene
Locus Name:
GeneID:
Location:

GeneID:1301
TrivialName: PA14_64220
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_64220
PAO1 Ortholog Locus:PA4855
Sequence Length:1290
Protein Length:429
Start: 5721422
Stop: 5722711
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG08240.1|AE004899_1 (AE004899) phosphoribosylamine--glycine ligase [Pseudomonas aeruginosa PAO1]
Identities = 427/429 (99%), Positives = 428/429 (99%)
Sequence:
ATGAACGTACTCATCATCGGCAGCGGCGGTCGTGAACATGCCCTGGCCTGGAAAGTCGCCCAGGACCCGCGC
GTGGCGAAGGTCTTCGTCGCCCCGGGCAACGCCGGCACCGCCACCGAAGCCAAGTGCGAGAACGTCGCCATC
GACGTGCTGGCCCTGGAGCAACTGGCCGACTTCGCCGCGAAGAACGTCCAGTTGACCATCGTCGGTCCCGAG
GCGCCGCTGGTGGCAGGCGTGGTCGACCTGTTCCGTGAGCGCGGCCTGGATATCTTCGGCCCGACCGCCGGC
GCCGCCCAGCTGGAAGGCTCGAAGGCCTTCACCAAGGACTTCCTCGCCCGCCACCGGATCCCCACCGCCGCC
TATCGCAACTTCACCGAGGTCGAGCCGGCCCTGGCCTACCTGCGCGAGCAGGGCGCACCCATCGTCATCAAG
GCCGATGGCCTGGCCGCGGGCAAGGGCGTGATCGTCGCCATGAACCTGGACGAAGCCGAAGCCGCCGTGCGC
GACATGCTCGCCGGCAATGCCTTCGGCGACGCCGGCTCGCGCGTGGTGATCGAGGAGTTCCTCGATGGCGAG
GAGGCCAGCTTCATCGTCATGGTCGACGGCCAGAACGTGCTGCCGATGGCCTCCAGCCAGGACCACAAGCGC
GTCGGCGATGGCGACAGCGGGCCGAACACCGGCGGCATGGGCGCCTACTCGCCGGCCCCGGTGGTCACCGCC
GAGGTCCACCAGCGGGTGCTGGACGAAGTGATCTACCCGACGGTGCGCGGCATGGCCGAGGAAGGCAACGTC
TATACCGGCTTCCTCTATGCCGGCCTGATGATCGACAAGAGCGGCGCGCCCAAGGTCATCGAATTCAACTGC
CGCTTCGGCGACCCGGAAACCCAGCCGATCATGGTCCGCCTGGAGTCCTCCCTGGTCCTGCTGGTCGAGGCC
GCCCTGGCCAAGGCGCTGGACAAGGTCGAGGCGACCTGGGACCCGCGTCCGACCGTAGGCGTGGTGCTGGCC
GCCGGCGGCTATCCGGGCGACTACGCCAAGGGCGAGGTCATCGAAGGCCTGGCCGAGGCCGCCGCTCTGGAC
GGCAAGGTGTTCCACGCCGGCACCGCGCTGAAGGATGGCCAGGTGGTCACCTCCGGCGGCCGCGTGCTCTGC
GCCACCGCCATCGGCGAGAGCGTATCCGCCGCCCAGCAACAGGCCTATCGCCTGGCCGAGAAGATCCGCTGG
AACGGCTGCTTCTACCGCAAGGACATCGGCTACCGTGCCATCGCCCGCGAGCGCGGCGAGTCCTGA
Translation:
MNVLIIGSGGREHALAWKVAQDPRVAKVFVAPGNAGTATEAKCENVAIDVLALEQLADFAAKNVQLTIVGPE
APLVAGVVDLFRERGLDIFGPTAGAAQLEGSKAFTKDFLARHRIPTAAYRNFTEVEPALAYLREQGAPIVIK
ADGLAAGKGVIVAMNLDEAEAAVRDMLAGNAFGDAGSRVVIEEFLDGEEASFIVMVDGQNVLPMASSQDHKR
VGDGDSGPNTGGMGAYSPAPVVTAEVHQRVLDEVIYPTVRGMAEEGNVYTGFLYAGLMIDKSGAPKVIEFNC
RFGDPETQPIMVRLESSLVLLVEAALAKALDKVEATWDPRPTVGVVLAAGGYPGDYAKGEVIEGLAEAAALD
GKVFHAGTALKDGQVVTSGGRVLCATAIGESVSAAQQQAYRLAEKIRWNGCFYRKDIGYRAIARERGES*
AnnotationID:1310GeneID:1301
AnnotatorUID: diggins
Modification Date/Time: 2005-04-26 06:06:50
Gene Name:purD
Confidence Code:2
GeneProduct:phosphoribosylamine--glycine ligase
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(18) Nucleotide biosynthesis and metabolism
Alternate Gene Product Name:phosphoribosylglycinamide synthetase
Functional Category Confidence Code:5
COGs:COG0151,pfam01071
Secondary Functional Category(ies):
EC Number:6.3.4.13
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAN70392.1|AE016681_1 (AE016792) phosphoribosylamine--glycine ligase [Pseudomonas putida
           KT2440]
          Length = 431

 Score =  746 bits (1927), Expect = 0.0
 Identities = 372/428 (86%), Positives = 400/428 (93%)

>gb|AAO58296.1| (AE016873) phosphoribosylamine--glycine ligase [Pseudomonas
           syringae pv. tomato str. DC3000]
          Length = 431

 Score =  744 bits (1922), Expect = 0.0
 Identities = 369/428 (86%), Positives = 400/428 (93%)

>dbj|BAC61161.1| (AP005083) phosphoribosylamine-glycine ligase [Vibrio
           parahaemolyticus]
          Length = 429

 Score =  644 bits (1661), Expect = 0.0
 Identities = 321/427 (75%), Positives = 363/427 (85%), Gaps = 1/427 (0%)






 
Structural Features:
COG0151, PurD, Phosphoribosylamine-glycine ligase [Nucleotide transport and metabolism] 
CD-Length = 428 residues,  99.8% aligned
Score =  615 bits (1588), Expect = 3e-177
Identities = 268/429 (62%), Positives = 316/429 (73%), Gaps = 5/429 (1%)

pfam01071, GARS_A, Phosphoribosylglycinamide synthetase, ATP-grasp (A) domain.
CD-Length = 140 residues, 100.0% aligned
Score =  213 bits (545), Expect = 3e-56
Identities = 81/140 (57%), Positives = 105/140 (75%)






   
Genomic Context:






   
Comment:


















    ReferenceID:2605




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:19747




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:35184




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:1301_cdd.html


    		




 
URL:

















    ReferenceID:35970




  
Author/Investigator(s):
        Aiba A, Mizobuchi K.





  
Title:
        Nucleotide sequence analysis of genes purH and purD involved in the de novo purine nucleotide biosynthesis of Escherichia coli.




  
PubMed:
        2687276


MedLine:
        




    
Source:
J Biol Chem. 1989 Dec 15;264(35):21239-46.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=2687276

















    ReferenceID:35973




  
Author/Investigator(s):
        Chopra AK, Peterson JW, Prasad R.





  
Title:
        Nucleotide sequence analysis of purH and purD genes from Salmonella typhimurium.




  
PubMed:
        1954258


MedLine:
        




    
Source:
Biochim Biophys Acta. 1991 Nov 11;1090(3):351-4.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=1954258









           
Homologs By Global Alignment 

           

       



     
Gene ID:1301
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
2364
                           PA4855_tr
                           translation of PA4855
                           429
                           99.53
                           99.76
                           0
                           2167.0
     

     
     
75824
                           gb|AAG08240.1|AE004899_1
                           (AE004899) phosphoribosylamine--glycine ligase [Pseudomonas aeruginosa PAO1]
                           429
                           99.53
                           99.76
                           0
                           2167.0
     

     
     
75825
                           gb|AAN70392.1|AE016681_1
                           (AE016792) phosphoribosylamine--glycine ligase [Pseudomonas putida KT2440]
                           431
                           86.31
                           92.80
                           2
                           1926.0
     

     
     
75826
                           gb|AAO58296.1|
                           (AE016873) phosphoribosylamine--glycine ligase [Pseudomonas syringae pv. tomato str. DC3000]
                           431
                           85.61
                           92.80
                           2
                           1922.0
     

     
     
75828
                           gb|AAF93450.1|
                           (AE004115) phosphoribosylamine--glycine ligase [Vibrio cholerae O1 biovar eltor str. N16961]
                           430
                           75.11
                           85.11
                           2
                           1662.0
     

     






      

          
GC ORFID: 44303How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA4855Glimmer Score: 

          
Start: 4985102Stop: 4986391

          
Length: 1290


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA4855 translation of PA4855Homolog Bit Score 839.0

          
Other Homologs:  psyr_15may02_Scaffold4_revised_gene5777 (ORF: BLASTX 751.0), t_PA4855 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGAACGTACTCATCATCGGCAGCGGCGGTCGTGAACATGCCCTGGCCTGGAAAGTCGCCCAGGACCCGCGC 
GTGGCGAAGGTCTTCGTCGCCCCGGGCAACGCCGGCACCGCCACCGAAGCCAAGTGCGAGAACGTCGCCATC 
GACGTGCTGGCCCTGGAGCAACTGGCCGACTTCGCCGCGAAGAACGTCCAGTTGACCATCGTCGGTCCCGAG 
GCGCCGCTGGTGGCAGGCGTGGTCGACCTGTTCCGTGAGCGCGGCCTGGATATCTTCGGCCCGACCGCCGGC 
GCCGCCCAGCTGGAAGGCTCGAAGGCCTTCACCAAGGACTTCCTCGCCCGCCACCGGATCCCCACCGCCGCC 
TATCGCAACTTCACCGAGGTCGAGCCGGCCCTGGCCTACCTGCGCGAGCAGGGCGCACCCATCGTCATCAAG 
GCCGATGGCCTGGCCGCGGGCAAGGGCGTGATCGTCGCCATGAACCTGGACGAAGCCGAAGCCGCCGTGCGC 
GACATGCTCGCCGGCAATGCCTTCGGCGACGCCGGCTCGCGCGTGGTGATCGAGGAGTTCCTCGATGGCGAG 
GAGGCCAGCTTCATCGTCATGGTCGACGGCCAGAACGTGCTGCCGATGGCCTCCAGCCAGGACCACAAGCGC 
GTCGGCGATGGCGACAGCGGGCCGAACACCGGCGGCATGGGCGCCTACTCGCCGGCCCCGGTGGTCACCGCC 
GAGGTCCACCAGCGGGTGCTGGACGAAGTGATCTACCCGACGGTGCGCGGCATGGCCGAGGAAGGCAACGTC 
TATACCGGCTTCCTCTATGCCGGCCTGATGATCGACAAGAGCGGCGCGCCCAAGGTCATCGAATTCAACTGC 
CGCTTCGGCGACCCGGAAACCCAGCCGATCATGGTCCGCCTGGAGTCCTCCCTGGTCCTGCTGGTCGAGGCC 
GCCCTGGCCAAGGCGCTGGACAAGGTCGAGGCGACCTGGGACCCGCGTCCGACCGTAGGCGTGGTGCTGGCC 
GCCGGCGGCTATCCGGGCGACTACGCCAAGGGCGAGGTCATCGAAGGCCTGGCCGAGGCCGCCGCTCTGGAC 
GGCAAGGTGTTCCACGCCGGCACCGCGCTGAAGGATGGCCAGGTGGTCACCTCCGGCGGCCGCGTGCTCTGC 
GCCACCGCCATCGGCGAGAGCGTATCCGCCGCCCAGCAACAGGCCTATCGCCTGGCCGAGAAGATCCGCTGG 
AACGGCTGCTTCTACCGCAAGGACATCGGCTACCGTGCCATCGCCCGCGAGCGCGGCGAGTCCTGA