ViewGene
Locus Name:
GeneID:
Location:

GeneID:1299
TrivialName: PA14_40290
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_40290
PAO1 Ortholog Locus:PA1871
Sequence Length:1257
Protein Length:418
Start: 3600471
Stop: 3599215
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAC12656.1| (U68175) staphylolytic protease preproenzyme LasA [Pseudomonas aeruginosa]
Identities = 413/418 (98%), Positives = 416/418 (99%)
Sequence:
ATGCAGCACAAAAGATCCCGCGCCCTGGCGAGCCCGCGCTCGCCGTTCCTCTTCGCCCTGCTGGCGCTCGCC
GTGGGCGGCACGGCCAACGCCCATGACGATGGCCTGCCGGCCTTCCGCTATTCGGCGGAGCTACTCGGCCAG
TTGCAATTGCCCAGCGTGGCCCTGCCGCTGAATGACGAACTGTTCCTCTACGGTCGCGACGCCGAGGCGTTC
GACCTCGAGGCCTACCTGGCCTTGAACGCGCCGGCGCTGCGCGACAAGAGCGAATACCTGGAGCACTGGAGC
GGCTACTACAGCATCAACCCGAAAGTGTTGTTGACCCTGATGGTCATGCAGTCCGGGCCGTTGGGTGCGCCG
GACGAGCGCGCCTTGGCGGCGCCGCTGGGGCGGCTGTCGGCGAAACGCGGCTTCGATGCCCAGGTACGCGAC
GTGTTGCAGCAGTTGTCGCGACGCTACTACGGTTTCGAGGAATATCAGTTGCGCCAGGCGGCTGCGCGCAAG
GCCGTCGGCGAGGACGGCCTGAACGCGGCATCGGCCGCGCTGCTCGGTCTGTTGCGAGAGGGGGCGAAGGCC
TCCGCCGTGCAAGGCGGCAATCCGCTCGGAGCCTACGCGCAGACCTTCCAGCGCCTGTTCGGCACCCCGGCC
GCGGAACTCCTGCAGCCGCGCAACCGCGTGGCCCGGCAACTCCAGGCGAAGGCCGCGCTGGCGCCGCCATCC
AACCTGATGCAATTGCCCTGGCGCCAGGGCTATTCCTGGCAGCCCAACGGAGCGCATTCGAACACCGGCTCG
GGCTATCCGTACTCGTCCTTCGATGCGTCCTACGACTGGCCGCGCTGGGGCAGTGCGACCTACAGCGTGGTC
GCCGCCCACGCCGGCACGGTACGGGTGTTGTCGCGCTGCCAGGTACGGGTGACCCACCCCAGCGGCTGGGCG
ACCAACTACTACCACATGGACCAGATCCAGGTGAGCAACGGCCAGCAGGTCAGCGCCGACACCAAGCTCGGC
GTCTATGCCAGCAACATCAACACCGCGCTCTGCGAGGGTGGCAGCTCCACCGGACCGCACCTGCATTTCTCG
CTGCTCTACAACGGCGCCTTCGTTTCCCTGCAGGGAGCCAGCTTCGGTCCGTACCGGATCAACGTCGGCACC
AGCAACTACGACAACGACTGTCGCCGCTACTATTTCTACAACCAGAGCGCCGGCACCACCCATTGCGCTTTC
CGCCCGTTGTACAACCCCGGCCTGGCGCTCTGA
Translation:
MQHKRSRALASPRSPFLFALLALAVGGTANAHDDGLPAFRYSAELLGQLQLPSVALPLNDELFLYGRDAEAF
DLEAYLALNAPALRDKSEYLEHWSGYYSINPKVLLTLMVMQSGPLGAPDERALAAPLGRLSAKRGFDAQVRD
VLQQLSRRYYGFEEYQLRQAAARKAVGEDGLNAASAALLGLLREGAKASAVQGGNPLGAYAQTFQRLFGTPA
AELLQPRNRVARQLQAKAALAPPSNLMQLPWRQGYSWQPNGAHSNTGSGYPYSSFDASYDWPRWGSATYSVV
AAHAGTVRVLSRCQVRVTHPSGWATNYYHMDQIQVSNGQQVSADTKLGVYASNINTALCEGGSSTGPHLHFS
LLYNGAFVSLQGASFGPYRINVGTSNYDNDCRRYYFYNQSAGTTHCAFRPLYNPGLAL*
AnnotationID:1308GeneID:1299
AnnotatorUID: diggins
Modification Date/Time: 2005-04-28 14:02:47
Gene Name:lasA
Confidence Code:1
GeneProduct:staphylolytic protease preproenzyme LasA
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(26) Translation, post-translational modification, degradation
Alternate Gene Product Name:LasA protease precursor
Functional Category Confidence Code:5
COGs:pfam01551
Secondary Functional Category(ies):secreted factors (toxins, enzymes, alginate)
EC Number:3.4.24.-
Status:ACTIVE
Pathway:






 
Homology:






 
Structural Features:
pfam01551, Peptidase_M37, Peptidase family M23/M37.
CD-Length = 83 residues,  94.0% aligned
Score = 79.5 bits (196), Expect = 8e-16
Identities = 28/86 (32%), Positives = 36/86 (41%), Gaps = 10/86 (11%)






   
Genomic Context:






   
Comment:


















    ReferenceID:2601




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:19743




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:37085




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:1299_cdd.html


    		




 
URL:

















    ReferenceID:37777




  
Author/Investigator(s):
        Darzins A, Peters JE, Galloway DR.





  
Title:
        Revised nucleotide sequence of the lasA gene from Pseudomonas aeruginosa PAO1.




  
PubMed:
        2123032


MedLine:
        




    
Source:
Nucleic Acids Res. 1990 Nov 11;18(21):6444.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=2123032









           
Homologs By Global Alignment 

           

       



     
Gene ID:1299
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
75806
                           gb|AAC12656.1|
                           (U68175) staphylolytic protease preproenzyme LasA [Pseudomonas aeruginosa]
                           418
                           98.80
                           99.52
                           0
                           2175.0
     

     
     
2362
                           PA1871_tr
                           translation of PA1871
                           418
                           98.56
                           99.04
                           0
                           2168.0
     

     
     
75807
                           gb|AAG05260.1|AE004613_5
                           (AE004613) LasA protease precursor [Pseudomonas aeruginosa PAO1]
                           418
                           98.56
                           99.04
                           0
                           2168.0
     

     
     
75808
                           emb|CAA39397.1|
                           (X55904) precursor protein [Pseudomonas aeruginosa]
                           418
                           87.32
                           87.79
                           48
                           1930.0
     

     
     
75809
                           gb|AAA25873.1|
                           (M20982) structural elastase [Pseudomonas aeruginosa]
                           418
                           86.12
                           86.60
                           41
                           1857.0
     

     






      

          
GC ORFID: 44301How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA1871Glimmer Score: 

          
Start: 1353439Stop: 1354695

          
Length: 1257


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA1871 translation of PA1871Homolog Bit Score 839.0

          
Other Homologs:  t_PA1871 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGCAGCACAAAAGATCCCGCGCCCTGGCGAGCCCGCGCTCGCCGTTCCTCTTCGCCCTGCTGGCGCTCGCC 
GTGGGCGGCACGGCCAACGCCCATGACGATGGCCTGCCGGCCTTCCGCTATTCGGCGGAGCTACTCGGCCAG 
TTGCAATTGCCCAGCGTGGCCCTGCCGCTGAATGACGAACTGTTCCTCTACGGTCGCGACGCCGAGGCGTTC 
GACCTCGAGGCCTACCTGGCCTTGAACGCGCCGGCGCTGCGCGACAAGAGCGAATACCTGGAGCACTGGAGC 
GGCTACTACAGCATCAACCCGAAAGTGTTGTTGACCCTGATGGTCATGCAGTCCGGGCCGTTGGGTGCGCCG 
GACGAGCGCGCCTTGGCGGCGCCGCTGGGGCGGCTGTCGGCGAAACGCGGCTTCGATGCCCAGGTACGCGAC 
GTGTTGCAGCAGTTGTCGCGACGCTACTACGGTTTCGAGGAATATCAGTTGCGCCAGGCGGCTGCGCGCAAG 
GCCGTCGGCGAGGACGGCCTGAACGCGGCATCGGCCGCGCTGCTCGGTCTGTTGCGAGAGGGGGCGAAGGCC 
TCCGCCGTGCAAGGCGGCAATCCGCTCGGAGCCTACGCGCAGACCTTCCAGCGCCTGTTCGGCACCCCGGCC 
GCGGAACTCCTGCAGCCGCGCAACCGCGTGGCCCGGCAACTCCAGGCGAAGGCCGCGCTGGCGCCGCCATCC 
AACCTGATGCAATTGCCCTGGCGCCAGGGCTATTCCTGGCAGCCCAACGGAGCGCATTCGAACACCGGCTCG 
GGCTATCCGTACTCGTCCTTCGATGCGTCCTACGACTGGCCGCGCTGGGGCAGTGCGACCTACAGCGTGGTC 
GCCGCCCACGCCGGCACGGTACGGGTGTTGTCGCGCTGCCAGGTACGGGTGACCCACCCCAGCGGCTGGGCG 
ACCAACTACTACCACATGGACCAGATCCAGGTGAGCAACGGCCAGCAGGTCAGCGCCGACACCAAGCTCGGC 
GTCTATGCCAGCAACATCAACACCGCGCTCTGCGAGGGTGGCAGCTCCACCGGACCGCACCTGCATTTCTCG 
CTGCTCTACAACGGCGCCTTCGTTTCCCTGCAGGGAGCCAGCTTCGGTCCGTACCGGATCAACGTCGGCACC 
AGCAACTACGACAACGACTGTCGCCGCTACTATTTCTACAACCAGAGCGCCGGCACCACCCATTGCGCTTTC 
CGCCCGTTGTACAACCCCGGCCTGGCGCTCTGA