ViewGene
Locus Name:
GeneID:
Location:

GeneID:1297
TrivialName: PA14_20040
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_20040
PAO1 Ortholog Locus:PA3405
Sequence Length:1332
Protein Length:443
Start: 1727320
Stop: 1728651
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG06793.1|AE004761_9 (AE004761) metalloprotease secretion protein [Pseudomonas aeruginosa PAO1]
Identities = 442/443 (99%), Positives = 442/443 (99%)
Sequence:
ATGAGCGGGCAGGCGCGGTTGCGCGAAGTCGGCGCCGGCGGCGCGGCGCTGGAACTGGACGAGAAGCGTTTT
TCCCGGCTCGGCTGGGGGCTGGTGCTGCTCGGCTTCGTCGGCTTTCTCCTGTGGGCCGGGTTGGCCCCGCTG
GACAAGGGTGTCGGGGTCTCCGGCACGGTGATGGTGGCCGGTAGCCGCAAGGCCGTGCAGCACCCTACCGGC
GGCCTGGTCAGGCACATCCGCGTGCACGAAGGCGAGCGGGTTGAGGCAGGACAGGTGCTGCTGGAGATGGAC
GCTACCCAGGCCCGGGCCCAGGCCGATGGCCTGTTCGCCCAGTACCTGGCGGCGCTGGCGAGTCTGGCGCGG
CTGAGCGCCGAGCGCGATGAAAAGGCGCGGATCGAGTTTCCCGCCGAGCTGCTGGCGCTGGACGACCCGCGC
CTGCCGACCTTGCTGGAACAGCAGCGGCAGTTGCACGACAGCCGTCGGCGGGCGCTGCGCCTGGAACTCGAT
GGTCTCGCCGAGACGGTCGCCGGCAGCCAGGCGCAACTGGACGGCCTGCAAGCCGCGCTGCGCAGCAAGGAG
CAGCAGCGCGTCGCCCTCGAGGAACAGTTGCGCGGCCTGCGCCAACTGGCCAGCGAGGGCTACGTGCCGCGC
AACCGCCTGCTCGACAGCGAGCGCCTGCTGGCCCAGGTCAACGGCGAGATCGCCGGCGACCTCGGCAGCCTC
GGCAGTACCCGCCGGCAGATCCTCGAACTGCGCCTGCGCATGGCCCAGCGGCGGGAGAAGTTCCAGGAAGAG
GTGCGCGCCAGCCTGGCCGACGCCCAGGTGCGCGCCGAGGAGTTGCGCAATCGCCTGGCCAGCGCGCGCTTC
GACCTGGCCAACAGCGAGGTACGGGCGCCGGTCGCCGGCCTGGTGGTCGGCCAGGAAGTGTTCACCGAGGGC
GGCGTGATCGCGCCCGGCCAGCAACTGATGGAAATCCTCCCGGAGCGCCAGCCGCTGCTGGTGGACGCTCGG
CTGCCGGTGGAGATGGTCGACAAGGTCCGCGTCGGCCTGCCGGTGGAGCTGATGTTCAGCGCCTTCAACCAG
AGCACTACGCCGCGGGTGGAGGGCGAAGTGACCCTGGTCTCGGCCGACCGCCTGCTGGACGAGCGCAGCGAG
GCGCCGTACTACCGGGTGCGCATCCGCGTAGGCGAGGAGGGCGTACGTCGCCTGGCCGGCCTGGAGATCCGC
CCGGGGATGCCGGTGGAGGCCTTCGTTCGCAGCGGCGAGCGCTCGCTGTTGAACTACCTGTTCAAGCCGCTG
GCCGACCGTACCCACCTGGCGCTGGGGGAAGAATGA
Translation:
MSGQARLREVGAGGAALELDEKRFSRLGWGLVLLGFVGFLLWAGLAPLDKGVGVSGTVMVAGSRKAVQHPTG
GLVRHIRVHEGERVEAGQVLLEMDATQARAQADGLFAQYLAALASLARLSAERDEKARIEFPAELLALDDPR
LPTLLEQQRQLHDSRRRALRLELDGLAETVAGSQAQLDGLQAALRSKEQQRVALEEQLRGLRQLASEGYVPR
NRLLDSERLLAQVNGEIAGDLGSLGSTRRQILELRLRMAQRREKFQEEVRASLADAQVRAEELRNRLASARF
DLANSEVRAPVAGLVVGQEVFTEGGVIAPGQQLMEILPERQPLLVDARLPVEMVDKVRVGLPVELMFSAFNQ
STTPRVEGEVTLVSADRLLDERSEAPYYRVRIRVGEEGVRRLAGLEIRPGMPVEAFVRSGERSLLNYLFKPL
ADRTHLALGEE*
AnnotationID:1306GeneID:1297
AnnotatorUID: diggins
Modification Date/Time: 2005-04-18 11:11:25
Gene Name:hasE
Confidence Code:2
GeneProduct:metalloprotease secretion protein
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(19) Protein secretion/export apparatus
Alternate Gene Product Name:membrane fusion protein
Functional Category Confidence Code:5
COGs:pfam00529
Secondary Functional Category(ies):membrane protein
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>dbj|BAA88493.1| (AB023289) membrane fusion protein [Pseudomonas fluorescens]
          Length = 443

 Score =  501 bits (1291), Expect = e-141
 Identities = 258/427 (60%), Positives = 329/427 (77%), Gaps = 2/427 (0%)

>emb|CAD13437.1| (AJ421517) putative metalloprotease secretion protein [Yersinia
           ruckeri]
          Length = 443

 Score =  451 bits (1159), Expect = e-126
 Identities = 238/441 (53%), Positives = 319/441 (72%), Gaps = 8/441 (1%)

>gb|AAO39141.1| (AY230750) putative type I secretion protein [Photorhabdus
           luminescens]
          Length = 442

 Score =  402 bits (1033), Expect = e-111
 Identities = 211/428 (49%), Positives = 293/428 (68%), Gaps = 1/428 (0%)








 
Structural Features:
pfam00529, HlyD, HlyD family secretion protein
CD-Length = 309 residues,  99.7% aligned
Score =  132 bits (333), Expect = 8e-32
Identities = 102/314 (32%), Positives = 153/314 (48%), Gaps = 10/314 (3%)






   
Genomic Context:






   
Comment:


















    ReferenceID:2597




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:19739




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:32159




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:1297_cdd.html


    		




 
URL:

















    ReferenceID:34068




  
Author/Investigator(s):
        Idei A, Kawai E, Akatsuka H, Omori K.





  
Title:
        Cloning and characterization of the Pseudomonas fluorescens ATP-binding cassette exporter, HasDEF, for the heme acquisition protein HasA.




  
PubMed:
        10601212


MedLine:
        




    
Source:
J Bacteriol. 1999 Dec;181(24):7545-51.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=10601212









           
Homologs By Global Alignment 

           

       



     
Gene ID:1297
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
2359
                           PA3405_tr
                           translation of PA3405
                           443
                           99.77
                           99.77
                           0
                           2171.0
     

     
     
75750
                           gb|AAG06793.1|AE004761_9
                           (AE004761) metalloprotease secretion protein [Pseudomonas aeruginosa PAO1]
                           443
                           99.77
                           99.77
                           0
                           2171.0
     

     
     
75752
                           dbj|BAA12016.1|
                           (D83582) metalloprotease transporter [Serratia marcescens]
                           445
                           59.32
                           75.95
                           4
                           1268.0
     

     
     
75751
                           dbj|BAA88493.1|
                           (AB023289) membrane fusion protein [Pseudomonas fluorescens]
                           445
                           58.65
                           75.05
                           4
                           1285.0
     

     
     
75756
                           emb|CAA57067.1|
                           (X81195) hasE [Serratia marcescens]
                           451
                           56.54
                           71.17
                           22
                           1123.0
     

     






      

          
GC ORFID: 44299How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA3405Glimmer Score: 

          
Start: 3226612Stop: 3225281

          
Length: 1332


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA3405 translation of PA3405Homolog Bit Score 840.0

          
Other Homologs:  t_PA1247 (ORF: BLASTX 424.0), psyr_15may02_Scaffold17_revised_gene2828 (ORF: BLASTX 387.0), t_PA3405 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGAGCGGGCAGGCGCGGTTGCGCGAAGTCGGCGCCGGCGGCGCGGCGCTGGAACTGGACGAGAAGCGTTTT 
TCCCGGCTCGGCTGGGGGCTGGTGCTGCTCGGCTTCGTCGGCTTTCTCCTGTGGGCCGGGTTGGCCCCGCTG 
GACAAGGGTGTCGGGGTCTCCGGCACGGTGATGGTGGCCGGTAGCCGCAAGGCCGTGCAGCACCCTACCGGC 
GGCCTGGTCAGGCACATCCGCGTGCACGAAGGCGAGCGGGTTGAGGCAGGACAGGTGCTGCTGGAGATGGAC 
GCTACCCAGGCCCGGGCCCAGGCCGATGGCCTGTTCGCCCAGTACCTGGCGGCGCTGGCGAGTCTGGCGCGG 
CTGAGCGCCGAGCGCGATGAAAAGGCGCGGATCGAGTTTCCCGCCGAGCTGCTGGCGCTGGACGACCCGCGC 
CTGCCGACCTTGCTGGAACAGCAGCGGCAGTTGCACGACAGCCGTCGGCGGGCGCTGCGCCTGGAACTCGAT 
GGTCTCGCCGAGACGGTCGCCGGCAGCCAGGCGCAACTGGACGGCCTGCAAGCCGCGCTGCGCAGCAAGGAG 
CAGCAGCGCGTCGCCCTCGAGGAACAGTTGCGCGGCCTGCGCCAACTGGCCAGCGAGGGCTACGTGCCGCGC 
AACCGCCTGCTCGACAGCGAGCGCCTGCTGGCCCAGGTCAACGGCGAGATCGCCGGCGACCTCGGCAGCCTC 
GGCAGTACCCGCCGGCAGATCCTCGAACTGCGCCTGCGCATGGCCCAGCGGCGGGAGAAGTTCCAGGAAGAG 
GTGCGCGCCAGCCTGGCCGACGCCCAGGTGCGCGCCGAGGAGTTGCGCAATCGCCTGGCCAGCGCGCGCTTC 
GACCTGGCCAACAGCGAGGTACGGGCGCCGGTCGCCGGCCTGGTGGTCGGCCAGGAAGTGTTCACCGAGGGC 
GGCGTGATCGCGCCCGGCCAGCAACTGATGGAAATCCTCCCGGAGCGCCAGCCGCTGCTGGTGGACGCTCGG 
CTGCCGGTGGAGATGGTCGACAAGGTCCGCGTCGGCCTGCCGGTGGAGCTGATGTTCAGCGCCTTCAACCAG 
AGCACTACGCCGCGGGTGGAGGGCGAAGTGACCCTGGTCTCGGCCGACCGCCTGCTGGACGAGCGCAGCGAG 
GCGCCGTACTACCGGGTGCGCATCCGCGTAGGCGAGGAGGGCGTACGTCGCCTGGCCGGCCTGGAGATCCGC 
CCGGGGATGCCGGTGGAGGCCTTCGTTCGCAGCGGCGAGCGCTCGCTGTTGAACTACCTGTTCAAGCCGCTG 
GCCGACCGTACCCACCTGGCGCTGGGGGAAGAATGA