ViewGene
Locus Name:
GeneID:
Location:

GeneID:1295
TrivialName: PA14_29420
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_29420
PAO1 Ortholog Locus:PA2682
Sequence Length:1242
Protein Length:413
Start: 2552717
Stop: 2551476
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG06070.1|AE004696_4 (AE004696) conserved hypothetical protein [Pseudomonas aeruginosa PAO1]
Identities = 413/413 (100%), Positives = 413/413 (100%)
Sequence:
ATGGGACAGTACGTCAGCATCGCCGCGTCCGACGGCAGCGGCCGGTTCGACGCGTATCTCGCGCTCCCCGCC
AGCGGCAAGGGGCCGGGCGTGGTGATCGGTCAGGAAATCTTCGGCGTCAACGCGAACATGCGCGCGGTGGCC
GACCTGTACGCCGAAGAGGGCTACGTGGCCCTGGTCCCCGACCTGTTCTGGCGGCTCCAGCCGGGGGTCGAC
CTGGGCTACGACGAAGCGGCCTTCGCCAAGGCCATCGAGCTGTTCCAGCGCATCGACCTGGACGCCGCGGTG
GACGACATCGCCGCCTGTATCGAGCACCTGCGCCAACGCGAGGAGGTGGTCCACGCCGGGATCGGCTTCGTC
GGCTTCTGCATGGGCGGCAAGCTGGCCTACCTCGCCGCGACGCGCACCGACGTCTCCTGCTCGGTGGGCTAC
TATGGCATGGGCATCGAGGCGCTGCTCGACGAGGCGAAACAGATCAAGGGCCGGCTGGTGCTGCATTTCGCC
GAACAGGACGCCTACTGCCCGCAGCAGGCCCGCGACGCGATCCTGCCCTGCCTGCGCAACCTGCCCAAGACC
GAGTTGTACCTCTACCCCGGCGTCGATCACGCCTTCGCCCGCGTCGGCGGCATGCACTTCGACAAACCGGCC
TACCTGATGGCCCACGAACGCAGCATCGCCGCCCTGAAGCGGGAGATCGGGCCGAACTTCGACCTCTCCGCG
CTGTGGGACGAGCATGTGCGCCACGAGTTCGATACCCGCGACGTCGCCGCGACCATGGCGACCATGGTCGCC
GAGCCCTACGTCAACCATGTCCCGACCCTCACCGGCGGCGTCGGCCAGCGCGAACTGAGCCGCTTCTACCGC
CATCACTTCATCCACGGCAACCCGCCGGACATGACCCTCACGCCGATTTCGCGGACGGTGGGGGCGCTGCAG
GTGGTCGACGAGTTCGTCATGCGCTTCACCCACAGCTGCGAGATCGACTGGCTGCTGCCCGGCGTCCCGCCC
ACCGGGCGCTTCGTGGAAATCCCCATGCTCGGCGTGGTGCGCTTTCGCGGCGACCGCCTTTACCACGAGCAC
ATCTATTGGGACCAGGCCGGCGTGCTGGTGCAGATCGGCCTGCTCGACCCGCAGGGCCTGCCGGTCGCGGGG
GTGGAAAGCGCGCGCAAGCTGCTCGACGAAAGCCTGCCGTCGAACCGCCTGATGGCGCGCTGGGCAGCCAGC
GAAGGACTCGGCCTCTAG
Translation:
MGQYVSIAASDGSGRFDAYLALPASGKGPGVVIGQEIFGVNANMRAVADLYAEEGYVALVPDLFWRLQPGVD
LGYDEAAFAKAIELFQRIDLDAAVDDIAACIEHLRQREEVVHAGIGFVGFCMGGKLAYLAATRTDVSCSVGY
YGMGIEALLDEAKQIKGRLVLHFAEQDAYCPQQARDAILPCLRNLPKTELYLYPGVDHAFARVGGMHFDKPA
YLMAHERSIAALKREIGPNFDLSALWDEHVRHEFDTRDVAATMATMVAEPYVNHVPTLTGGVGQRELSRFYR
HHFIHGNPPDMTLTPISRTVGALQVVDEFVMRFTHSCEIDWLLPGVPPTGRFVEIPMLGVVRFRGDRLYHEH
IYWDQAGVLVQIGLLDPQGLPVAGVESARKLLDESLPSNRLMARWAASEGLGL*
AnnotationID:1304GeneID:1295
AnnotatorUID: diggins
Modification Date/Time: 2005-06-09 20:08:14
Gene Name:
Confidence Code:3
GeneProduct:putative dienelactone hydrolase
Cell Localization:()
Synonyms:ysgA
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(20) Putative enzymes
Alternate Gene Product Name:carboxymethylenebutenolidase
Functional Category Confidence Code:5
COGs:COG0412,pfam01738
Secondary Functional Category(ies):Secondary metabolites biosynthesis, transport, and catabolism
EC Number:3.1.1.45
Status:ACTIVE
Pathway:
gamma-Hexachlorocyclohexane degradation;
1,4-Dichlorobenzene degradation
Homology:
>dbj|BAC41531.1| (AB088420) dienelactone hydrolase [Pseudomonas resinovorans]
          Length = 411

 Score =  628 bits (1620), Expect = e-179
 Identities = 301/408 (73%), Positives = 347/408 (85%)
Structural Features:
COG0412, Dienelactone hydrolase and related enzymes 
[Secondary metabolites biosynthesis, transport, and catabolism]
CD-Length = 236 residues,  99.6% aligned
Score =  132 bits (331), Expect = 9e-32
Identities = 92/240 (38%), Positives = 127/240 (52%), Gaps = 10/240 (4%)

pfam01738, DLH, Dienelactone hydrolase family. 
CD-Length = 216 residues,  99.1% aligned
Score =  138 bits (347), Expect = 1e-33
Identities = 91/219 (41%), Positives = 119/219 (54%), Gaps = 9/219 (4%)
Genomic Context:






   
Comment:


















    ReferenceID:2593




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:19735




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:36335




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:1295_cdd.html


    		




 
URL:









           
Homologs By Global Alignment 

           

       



     
Gene ID:1295
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
2357
                           PA2682_tr
                           translation of PA2682
                           413
                           99.99
                           99.99
                           0
                           2173.0
     

     
     
75707
                           gb|AAG06070.1|AE004696_4
                           (AE004696) conserved hypothetical protein [Pseudomonas aeruginosa PAO1]
                           413
                           99.99
                           99.99
                           0
                           2173.0
     

     
     
75708
                           dbj|BAC41531.1|
                           (AB088420) dienelactone hydrolase [Pseudomonas resinovorans]
                           413
                           72.88
                           84.01
                           2
                           1615.0
     

     
     
75709
                           dbj|BAB32752.1|
                           (AB047548) dienelactone hydrolase [Pseudomonas resinovorans]
                           413
                           44.79
                           52.30
                           140
                           966.0
     

     
     
75711
                           dbj|BAC91681.1|
                           (AP006581) carboxymethylenebutenolidase [Gloeobacter violaceus PCC 7421]
                           413
                           25.90
                           34.86
                           183
                           513.0
     

     






      

          
GC ORFID: 44297How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA2682Glimmer Score: 

          
Start: 2401209Stop: 2402450

          
Length: 1242


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TAGTruncated Stop: 

          
Homolog: t_PA2682 translation of PA2682Homolog Bit Score 841.0

          
Other Homologs:  t_PA2682 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGGGACAGTACGTCAGCATCGCCGCGTCCGACGGCAGCGGCCGGTTCGACGCGTATCTCGCGCTCCCCGCC 
AGCGGCAAGGGGCCGGGCGTGGTGATCGGTCAGGAAATCTTCGGCGTCAACGCGAACATGCGCGCGGTGGCC 
GACCTGTACGCCGAAGAGGGCTACGTGGCCCTGGTCCCCGACCTGTTCTGGCGGCTCCAGCCGGGGGTCGAC 
CTGGGCTACGACGAAGCGGCCTTCGCCAAGGCCATCGAGCTGTTCCAGCGCATCGACCTGGACGCCGCGGTG 
GACGACATCGCCGCCTGTATCGAGCACCTGCGCCAACGCGAGGAGGTGGTCCACGCCGGGATCGGCTTCGTC 
GGCTTCTGCATGGGCGGCAAGCTGGCCTACCTCGCCGCGACGCGCACCGACGTCTCCTGCTCGGTGGGCTAC 
TATGGCATGGGCATCGAGGCGCTGCTCGACGAGGCGAAACAGATCAAGGGCCGGCTGGTGCTGCATTTCGCC 
GAACAGGACGCCTACTGCCCGCAGCAGGCCCGCGACGCGATCCTGCCCTGCCTGCGCAACCTGCCCAAGACC 
GAGTTGTACCTCTACCCCGGCGTCGATCACGCCTTCGCCCGCGTCGGCGGCATGCACTTCGACAAACCGGCC 
TACCTGATGGCCCACGAACGCAGCATCGCCGCCCTGAAGCGGGAGATCGGGCCGAACTTCGACCTCTCCGCG 
CTGTGGGACGAGCATGTGCGCCACGAGTTCGATACCCGCGACGTCGCCGCGACCATGGCGACCATGGTCGCC 
GAGCCCTACGTCAACCATGTCCCGACCCTCACCGGCGGCGTCGGCCAGCGCGAACTGAGCCGCTTCTACCGC 
CATCACTTCATCCACGGCAACCCGCCGGACATGACCCTCACGCCGATTTCGCGGACGGTGGGGGCGCTGCAG 
GTGGTCGACGAGTTCGTCATGCGCTTCACCCACAGCTGCGAGATCGACTGGCTGCTGCCCGGCGTCCCGCCC 
ACCGGGCGCTTCGTGGAAATCCCCATGCTCGGCGTGGTGCGCTTTCGCGGCGACCGCCTTTACCACGAGCAC 
ATCTATTGGGACCAGGCCGGCGTGCTGGTGCAGATCGGCCTGCTCGACCCGCAGGGCCTGCCGGTCGCGGGG 
GTGGAAAGCGCGCGCAAGCTGCTCGACGAAAGCCTGCCGTCGAACCGCCTGATGGCGCGCTGGGCAGCCAGC 
GAAGGACTCGGCCTCTAG