ViewGene
Locus Name:
GeneID:
Location:

GeneID:1291
TrivialName: PA14_66440
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_66440
PAO1 Ortholog Locus:PA5025
Sequence Length:1278
Protein Length:425
Start: 5927048
Stop: 5928325
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG08410.1|AE004915_5 (AE004915) homocysteine synthase [Pseudomonas aeruginosa PAO1]
Identities = 424/425 (99%), Positives = 425/425 (100%)
Sequence:
ATGAAACTGGAAACCCTGGCCGTCCACGCCGGCTACAGCCCTGACCCGACCACCCGCGCGGTGGCGGTGCCG
ATCTACCAGACCACTTCCTACGCCTTCGACGACACCCAGCATGGCGCCGACCTGTTCGACCTGAAGGTACCG
GGCAACATCTACACACGGATCATGAACCCCACCAACGACGTACTGGAACAGCGCGTCGCGGCGCTGGAAGGC
GGGGTCGGGGCGCTGGCGGTGGCCTCGGGGATGGCGGCCATCACCTACGCGATCCAGACCGTCGCCGAGGCC
GGCGACAACATCGTCTCGGTGGCCAAGCTCTACGGCGGCACCTACAACCTGCTGGCCCACACCCTGCCACGC
ATCGGCATCCAGGCGCGCTTCGCCGCCCACGACGACGTCGCCGCCCTGGAAGCGCTGATTGACGAGCGGACC
AAGGCCGTGTTCTGCGAAACCATCGGCAACCCGGCGGGCAACATCATCGACCTGCAGGCACTGGCCGACGCC
GCTCACCGCCACGGCGTGCCACTGATCGTCGACAACACGGTAGCCACCCCGGTGCTCTGCCGGCCGTTCGAG
CACGGCGCCGACATCGTCGTGCACTCGCTGACCAAGTACATGGGCGGCCACGGCACCAGCATCGGCGGGATC
GTGGTCGACTCCGGCAAATTCGACTGGGCGGCGAACAAGTCGCGCTTCCCGCTGCTGAACACGCCCGATCCG
TCCTACCACGGCGTCACCTACACCGAGGCCTTCGGACCCGCCGCCTTCATCGGCCGCTGCCGGGTGGTACCG
CTGCGCAACATGGGCGCGGCGCTCTCGCCGTTCAACGCCTTCCTCATCCTCCAAGGCCTGGAGACCCTGGCG
CTGCGCATGGAGCGCCACTGCGACAACGCCCTCGCCGTGGCCCGCTACCTGCAGCAGCATCCGCAGGTGGCC
TGGGTGAAATACGCCGGCCTCGCCGACAACCCCGAGCACGCCCTGGCCCGGCGCTACCTGGGGGGCCGCCCG
GCGGCGATCCTGTCCTTCGGTATCCAGGGCGGCAGCGCCGCTGGCGCGCGCTTCATCGACGCCTTGAAGCTG
GTGGTGCGGCTGGTCAACATCGGCGACGCCAAGTCCCTGGCCTGCCACCCGGCGAGCACCACCCACCGCCAG
TTGAACGCGGAGGAACTGGCCCGCGCCGGAGTCTCCGACGACATGGTGCGGCTGTCGATCGGCATCGAGCAC
ATCAACGACATCCTCGCCGACCTCGACCAGGCCCTGGCCGCCGCCGCACGCTGA
Translation:
MKLETLAVHAGYSPDPTTRAVAVPIYQTTSYAFDDTQHGADLFDLKVPGNIYTRIMNPTNDVLEQRVAALEG
GVGALAVASGMAAITYAIQTVAEAGDNIVSVAKLYGGTYNLLAHTLPRIGIQARFAAHDDVAALEALIDERT
KAVFCETIGNPAGNIIDLQALADAAHRHGVPLIVDNTVATPVLCRPFEHGADIVVHSLTKYMGGHGTSIGGI
VVDSGKFDWAANKSRFPLLNTPDPSYHGVTYTEAFGPAAFIGRCRVVPLRNMGAALSPFNAFLILQGLETLA
LRMERHCDNALAVARYLQQHPQVAWVKYAGLADNPEHALARRYLGGRPAAILSFGIQGGSAAGARFIDALKL
VVRLVNIGDAKSLACHPASTTHRQLNAEELARAGVSDDMVRLSIGIEHINDILADLDQALAAAAR*
AnnotationID:1300GeneID:1291
AnnotatorUID: diggins
Modification Date/Time: 2005-04-26 10:10:30
Gene Name:metY
Confidence Code:2
GeneProduct:homocysteine synthase
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(2) Amino acid biosynthesis and metabolism
Alternate Gene Product Name:O-acetylhomoserine sulfhydrylase
Functional Category Confidence Code:5
COGs:COG2873,pfam01053,COG0626
Secondary Functional Category(ies):central intermediary metabolism
EC Number:4.2.99.10
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAN68137.1|AE016445_7 (AE016783) O-acetylhomoserine sulfhydrylase [Pseudomonas putida
           KT2440]
          Length = 425

 Score =  749 bits (1934), Expect = 0.0
 Identities = 371/423 (87%), Positives = 398/423 (94%)

>gb|AAN54166.1|AE015554_1 (AE015554) O-acetylhomoserine (thiol)-lyase, putative [Shewanella
           oneidensis MR-1]
          Length = 430

 Score =  649 bits (1674), Expect = 0.0
 Identities = 315/428 (73%), Positives = 363/428 (84%), Gaps = 5/428 (1%)

>emb|CAE31553.1| (BX640440) O-acetylhomoserine sulfhydrylase [Bordetella
           bronchiseptica]
          Length = 431

 Score =  628 bits (1620), Expect = e-179
 Identities = 305/420 (72%), Positives = 357/420 (85%)






 
Structural Features:
COG2873, MET17, O-acetylhomoserine sulfhydrylase [Amino acid transport and metabolism]
CD-Length = 426 residues,  98.8% aligned
Score =  674 bits (1740), Expect = 0.0
Identities = 295/423 (69%), Positives = 340/423 (80%), Gaps = 2/423 (0%)

pfam01053, Cys_Met_Meta_PP, Cys/Met metabolism PLP-dependent enzyme.
CD-Length = 384 residues, 100.0% aligned
Score =  450 bits (1160), Expect = 1e-127
Identities = 189/420 (45%), Positives = 255/420 (60%), Gaps = 38/420 (9%)

COG0626, MetC, Cystathionine beta-lyases/cystathionine gamma-synthases 
[Amino acid transport and metabolism]
CD-Length = 396 residues,  98.7% aligned
Score =  406 bits (1044), Expect = 4e-114
Identities = 180/425 (42%), Positives = 246/425 (57%), Gaps = 35/425 (8%)






   
Genomic Context:






   
Comment:


















    ReferenceID:2585




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:19727




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:35040




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:1291_cdd.html


    		




 
URL:

















    ReferenceID:35860




  
Author/Investigator(s):
        Belfaiza J, Martel A, Margarita D, Saint Girons I.





  
Title:
        Direct sulfhydrylation for methionine biosynthesis in Leptospira meyeri.




  
PubMed:
        9440513


MedLine:
        




    
Source:
J Bacteriol. 1998 Jan;180(2):250-5.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=9440513

















    ReferenceID:35861




  
Author/Investigator(s):
        Hwang BJ, Yeom HJ, Kim Y, Lee HS.





  
Title:
        Corynebacterium glutamicum utilizes both transsulfuration and direct sulfhydrylation pathways for methionine biosynthesis.




  
PubMed:
        11844756


MedLine:
        




    
Source:
J Bacteriol. 2002 Mar;184(5):1277-86.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=11844756









           
Homologs By Global Alignment 

           

       



     
Gene ID:1291
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
2352
                           PA5025_tr
                           translation of PA5025
                           425
                           99.76
                           99.99
                           0
                           2174.0
     

     
     
75534
                           gb|AAG08410.1|AE004915_5
                           (AE004915) homocysteine synthase [Pseudomonas aeruginosa PAO1]
                           425
                           99.76
                           99.99
                           0
                           2174.0
     

     
     
75535
                           gb|AAN68137.1|AE016445_7
                           (AE016783) O-acetylhomoserine sulfhydrylase [Pseudomonas putida KT2440]
                           425
                           87.29
                           93.64
                           0
                           1931.0
     

     
     
75536
                           emb|CAE15890.1|
                           (BX571870) unnamed protein product [Photorhabdus luminescens subsp. laumondii TTO1]
                           425
                           80.00
                           89.64
                           1
                           1816.0
     

     
     
75537
                           gb|AAQ59608.1|
                           (AE016916) O-acetylhomoserine (thiol)-lyase [Chromobacterium violaceum ATCC 12472]
                           425
                           74.82
                           85.41
                           0
                           1680.0
     

     






      

          
GC ORFID: 44293How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA5025Glimmer Score: 

          
Start: 5190731Stop: 5192008

          
Length: 1278


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA5025 translation of PA5025Homolog Bit Score 842.0

          
Other Homologs:  t_PA5025 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGAAACTGGAAACCCTGGCCGTCCACGCCGGCTACAGCCCTGACCCGACCACCCGCGCGGTGGCGGTGCCG 
ATCTACCAGACCACTTCCTACGCCTTCGACGACACCCAGCATGGCGCCGACCTGTTCGACCTGAAGGTACCG 
GGCAACATCTACACACGGATCATGAACCCCACCAACGACGTACTGGAACAGCGCGTCGCGGCGCTGGAAGGC 
GGGGTCGGGGCGCTGGCGGTGGCCTCGGGGATGGCGGCCATCACCTACGCGATCCAGACCGTCGCCGAGGCC 
GGCGACAACATCGTCTCGGTGGCCAAGCTCTACGGCGGCACCTACAACCTGCTGGCCCACACCCTGCCACGC 
ATCGGCATCCAGGCGCGCTTCGCCGCCCACGACGACGTCGCCGCCCTGGAAGCGCTGATTGACGAGCGGACC 
AAGGCCGTGTTCTGCGAAACCATCGGCAACCCGGCGGGCAACATCATCGACCTGCAGGCACTGGCCGACGCC 
GCTCACCGCCACGGCGTGCCACTGATCGTCGACAACACGGTAGCCACCCCGGTGCTCTGCCGGCCGTTCGAG 
CACGGCGCCGACATCGTCGTGCACTCGCTGACCAAGTACATGGGCGGCCACGGCACCAGCATCGGCGGGATC 
GTGGTCGACTCCGGCAAATTCGACTGGGCGGCGAACAAGTCGCGCTTCCCGCTGCTGAACACGCCCGATCCG 
TCCTACCACGGCGTCACCTACACCGAGGCCTTCGGACCCGCCGCCTTCATCGGCCGCTGCCGGGTGGTACCG 
CTGCGCAACATGGGCGCGGCGCTCTCGCCGTTCAACGCCTTCCTCATCCTCCAAGGCCTGGAGACCCTGGCG 
CTGCGCATGGAGCGCCACTGCGACAACGCCCTCGCCGTGGCCCGCTACCTGCAGCAGCATCCGCAGGTGGCC 
TGGGTGAAATACGCCGGCCTCGCCGACAACCCCGAGCACGCCCTGGCCCGGCGCTACCTGGGGGGCCGCCCG 
GCGGCGATCCTGTCCTTCGGTATCCAGGGCGGCAGCGCCGCTGGCGCGCGCTTCATCGACGCCTTGAAGCTG 
GTGGTGCGGCTGGTCAACATCGGCGACGCCAAGTCCCTGGCCTGCCACCCGGCGAGCACCACCCACCGCCAG 
TTGAACGCGGAGGAACTGGCCCGCGCCGGAGTCTCCGACGACATGGTGCGGCTGTCGATCGGCATCGAGCAC 
ATCAACGACATCCTCGCCGACCTCGACCAGGCCCTGGCCGCCGCCGCACGCTGA