ViewGene
Locus Name:
GeneID:
Location:

GeneID:1285
TrivialName: PA14_65300
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_65300
PAO1 Ortholog Locus:PA4943
Sequence Length:1302
Protein Length:433
Start: 5819774
Stop: 5818473
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG08328.1|AE004907_6 (AE004907) probable GTP-binding protein [Pseudomonas aeruginosa PAO1]
Identities = 432/433 (99%), Positives = 433/433 (100%)
Sequence:
TTGTTCTTTGAGCGCCCAGGTGGTGGGGAACAGGCTGTTCTGGTGCATCTGGAAGGGCAGAACCCCGAGGCG
CGTGAAGACCCGCAGGAGTTCCAGGAGCTGGTGCGTTCGGCCGGCGCGGAAACGGTGGCATTCGTCAGCGTC
TCCCGCCACCAGCCGAGCGCCAAATATCTTATCGGCAGTGGCAAGGTCGAGGAGTTGCACGACCTGGTCAAG
GCCGAAAACGTCGAGCTGGTGATTTTCAACCACACCTTGACGCCCAGCCAGGAGCGCAACCTCGAGCGAGCG
TTCGAGTGCCGCGTACTCGACCGGACCGGGCTGATCCTCGACATCTTCGCCCAGCGCGCACGTACCCACGAA
GGCAAGTTGCAGGTGGAACTGGCGCAACTGGAGCACATGAGCACGCGGCTGGTACGCGGCTGGACCCACCTC
GAACGGCAGAAAGGCGGTATCGGCCTGCGCGGTCCGGGTGAGACCCAGCTGGAAACCGACCGACGCCTGCTG
CGCGTGCGCATCCGCCAGATCAAGCAGCGCCTGGAGAAGGTCCGCAGCCAGCGCGAGCAGGCTCGACGCGGG
CGCAAGCGCGCGGATATCCCGGCGGTCTCCCTGGTCGGCTATACCAACGCCGGCAAATCGACCCTGTTCAAT
GCGCTCACCGCCTCCGAGGTGTATGCCGCCAACCAGTTGTTCGCCACCCTCGACCCCACCCTGCGTCGCCTG
CAACTGGACGATCTCGGGCCGGTGGTGCTGGCCGACACGGTGGGCTTCATCCGTCACCTGCCGCACAAGCTG
GTGGAGGCCTTCCGCGCTACGCTGGAAGAGTCGAGCAACGCCGACCTCCTGCTGCACGTGATCGACGCCTAC
GAGCCCGAACGGGATGCGCAGGTCGAGCAGGTGCTGGCGGTGCTCGGCGAGATCGGCGCCAACGAATTGCCG
ATGCTCGAGGTCTATAACAAGGTCGACCTGCTACCCTCGGTCGAGCCGCACATCCAGCGCGACGACTCCGGC
AAGCCGGTGCGCGTCTGGCTGTCGGCGCAGACCGGCGAAGGCCTCGACCTGTTGCGCCAGGCCATCGCCGAG
CTGCTCGGCGAGGACCTCTTCGTCGGGACCTTGCGCCTGCCCCAGCGGCTCGGGCGCCTGCGCGCCCAGTTG
TTCGAACTGGGCGCGGTGCAGTCCGAGGCGCACGATGAGGAAGGTTGTACCCTGCTGCAGGTGCGCCTGCCG
CGGGCCGAGTTGAACCGCCTGGCGAGCCGCGCTGGCTGGCAGCCGGCGGAGTTCGTTGCGCAACACACTTTG
CAATAA
Translation:
MFFERPGGGEQAVLVHLEGQNPEAREDPQEFQELVRSAGAETVAFVSVSRHQPSAKYLIGSGKVEELHDLVK
AENVELVIFNHTLTPSQERNLERAFECRVLDRTGLILDIFAQRARTHEGKLQVELAQLEHMSTRLVRGWTHL
ERQKGGIGLRGPGETQLETDRRLLRVRIRQIKQRLEKVRSQREQARRGRKRADIPAVSLVGYTNAGKSTLFN
ALTASEVYAANQLFATLDPTLRRLQLDDLGPVVLADTVGFIRHLPHKLVEAFRATLEESSNADLLLHVIDAY
EPERDAQVEQVLAVLGEIGANELPMLEVYNKVDLLPSVEPHIQRDDSGKPVRVWLSAQTGEGLDLLRQAIAE
LLGEDLFVGTLRLPQRLGRLRAQLFELGAVQSEAHDEEGCTLLQVRLPRAELNRLASRAGWQPAEFVAQHTL
Q*
AnnotationID:1294GeneID:1285
AnnotatorUID: diggins
Modification Date/Time: 2005-03-13 14:02:32
Gene Name:hflX
Confidence Code:2
GeneProduct:putative GTP-binding protein
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(20) Putative enzymes
Alternate Gene Product Name:putative GTP binding subunit for protease of lambda cII repressor
Functional Category Confidence Code:5
COGs:COG2262
Secondary Functional Category(ies):transport of small molecules
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAN70460.1|AE016687_7 (AE016792) GTP-binding protein HflX [Pseudomonas putida KT2440]
          Length = 433

 Score =  717 bits (1851), Expect = 0.0
 Identities = 367/433 (84%), Positives = 393/433 (90%)

>gb|AAO58369.1| (AE016873) GTP-binding protein HflX [Pseudomonas syringae pv.
           tomato str. DC3000]
          Length = 433

 Score =  716 bits (1848), Expect = 0.0
 Identities = 360/433 (83%), Positives = 397/433 (91%)

>gb|AAO58369.1| (AE016873) GTP-binding protein HflX [Pseudomonas syringae pv.
           tomato str. DC3000]
          Length = 433

 Score =  716 bits (1848), Expect = 0.0
 Identities = 360/433 (83%), Positives = 397/433 (91%)

>gb|AAN53682.1|AE015507_8 (AE015507) GTP-binding protein HflX [Shewanella oneidensis MR-1]
          Length = 435

 Score =  499 bits (1285), Expect = e-140
 Identities = 264/431 (61%), Positives = 319/431 (74%), Gaps = 1/431 (0%)






 
Structural Features:
COG2262, HflX, GTPases
CD-Length = 411 residues,  99.5% aligned
Score =  465 bits (1197), Expect = 6e-132
Identities = 215/412 (52%), Positives = 272/412 (66%), Gaps = 3/412 (0%)







   
Genomic Context:






   
Comment:


















    ReferenceID:14323




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:19715




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:35142




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:1285_cdd.html


    		




 
URL:

















    ReferenceID:35905




  
Author/Investigator(s):
        Noble JA, Innis MA, Koonin EV, Rudd KE, Banuett F, Herskowitz I.





  
Title:
        The Escherichia coli hflA locus encodes a putative GTP-binding protein and two membrane proteins, one of which contains a protease-like domain.




  
PubMed:
        8248183


MedLine:
        




    
Source:
Proc Natl Acad Sci U S A. 1993 Nov 15;90(22):10866-70.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=8248183









           
Homologs By Global Alignment 

           

       



     
Gene ID:1285
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
2343
                           PA4943_tr
                           translation of PA4943
                           433
                           99.76
                           99.99
                           0
                           2179.0
     

     
     
75294
                           gb|AAG08328.1|AE004907_6
                           (AE004907) probable GTP-binding protein [Pseudomonas aeruginosa PAO1]
                           433
                           99.53
                           99.99
                           0
                           2177.0
     

     
     
75295
                           gb|AAN70460.1|AE016687_7
                           (AE016792) GTP-binding protein HflX [Pseudomonas putida KT2440]
                           433
                           84.52
                           90.76
                           0
                           1848.0
     

     
     
75296
                           gb|AAO58369.1|
                           (AE016873) GTP-binding protein HflX [Pseudomonas syringae pv. tomato str. DC3000]
                           433
                           82.90
                           91.68
                           0
                           1845.0
     

     
     
75297
                           gb|AAN53682.1|AE015507_8
                           (AE015507) GTP-binding protein HflX [Shewanella oneidensis MR-1]
                           437
                           60.41
                           72.99
                           6
                           1287.0
     

     






      

          
GC ORFID: 44287How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA4943Glimmer Score: 

          
Start: 5083455Stop: 5082154

          
Length: 1302


          
Start Codon: TTGTruncated Start:

          
Stop Codon: TAATruncated Stop: 

          
Homolog: t_PA4943 translation of PA4943Homolog Bit Score 843.0

          
Other Homologs:  psyr_15may02_Scaffold2_revised_gene3431 (ORF: BLASTX 719.0), t_PA4943 (ORF: GLIMMER)

          
GC ORF Sequence

           
TTGTTCTTTGAGCGCCCAGGTGGTGGGGAACAGGCTGTTCTGGTGCATCTGGAAGGGCAGAACCCCGAGGCG 
CGTGAAGACCCGCAGGAGTTCCAGGAGCTGGTGCGTTCGGCCGGCGCGGAAACGGTGGCATTCGTCAGCGTC 
TCCCGCCACCAGCCGAGCGCCAAATATCTTATCGGCAGTGGCAAGGTCGAGGAGTTGCACGACCTGGTCAAG 
GCCGAAAACGTCGAGCTGGTGATTTTCAACCACACCTTGACGCCCAGCCAGGAGCGCAACCTCGAGCGAGCG 
TTCGAGTGCCGCGTACTCGACCGGACCGGGCTGATCCTCGACATCTTCGCCCAGCGCGCACGTACCCACGAA 
GGCAAGTTGCAGGTGGAACTGGCGCAACTGGAGCACATGAGCACGCGGCTGGTACGCGGCTGGACCCACCTC 
GAACGGCAGAAAGGCGGTATCGGCCTGCGCGGTCCGGGTGAGACCCAGCTGGAAACCGACCGACGCCTGCTG 
CGCGTGCGCATCCGCCAGATCAAGCAGCGCCTGGAGAAGGTCCGCAGCCAGCGCGAGCAGGCTCGACGCGGG 
CGCAAGCGCGCGGATATCCCGGCGGTCTCCCTGGTCGGCTATACCAACGCCGGCAAATCGACCCTGTTCAAT 
GCGCTCACCGCCTCCGAGGTGTATGCCGCCAACCAGTTGTTCGCCACCCTCGACCCCACCCTGCGTCGCCTG 
CAACTGGACGATCTCGGGCCGGTGGTGCTGGCCGACACGGTGGGCTTCATCCGTCACCTGCCGCACAAGCTG 
GTGGAGGCCTTCCGCGCTACGCTGGAAGAGTCGAGCAACGCCGACCTCCTGCTGCACGTGATCGACGCCTAC 
GAGCCCGAACGGGATGCGCAGGTCGAGCAGGTGCTGGCGGTGCTCGGCGAGATCGGCGCCAACGAATTGCCG 
ATGCTCGAGGTCTATAACAAGGTCGACCTGCTACCCTCGGTCGAGCCGCACATCCAGCGCGACGACTCCGGC 
AAGCCGGTGCGCGTCTGGCTGTCGGCGCAGACCGGCGAAGGCCTCGACCTGTTGCGCCAGGCCATCGCCGAG 
CTGCTCGGCGAGGACCTCTTCGTCGGGACCTTGCGCCTGCCCCAGCGGCTCGGGCGCCTGCGCGCCCAGTTG 
TTCGAACTGGGCGCGGTGCAGTCCGAGGCGCACGATGAGGAAGGTTGTACCCTGCTGCAGGTGCGCCTGCCG 
CGGGCCGAGTTGAACCGCCTGGCGAGCCGCGCTGGCTGGCAGCCGGCGGAGTTCGTTGCGCAACACACTTTG 
CAATAA