ViewGene
Locus Name:
GeneID:
Location:

GeneID:1282
TrivialName: PA14_72280
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_72280
PAO1 Ortholog Locus:PA5476
Sequence Length:1290
Protein Length:429
Start: 6440326
Stop: 6439037
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG08861.1|AE004960_4 (AE004960) citrate transporter [Pseudomonas aeruginosa PAO1]
Identities = 429/429 (100%), Positives = 429/429 (100%)
Sequence:
ATGGCCTCTAATAAATCCAAAGGCGTCGCCATTTTCCGTGTGGTCAGTGGCAACTTCCTCGAGATGTACGAC
TTCATGGTGTATGGCTTCTACGCCACCGCCATCGCCAAGACCTTCTTCCCCGGCGACAACCCGTTCGCCTCC
CTGATGCTGTCCCTCGCCACCTTTGGCGCCGGCTTCCTGATGCGGCCGTTGGGTGCGGTGTTCCTGGGCGCC
TACATCGATCGCCACGGCCGCCGCCAGGGCCTGATCATCACCCTCGGCCTGATGGCCATGGGCACCCTGCTG
ATCGCCTTCGTTCCCGGCTATGCCACGCTGGGCGTGGCGGCGCCGTTGCTGGTGCTGTTCGGCCGGCTCCTG
CAGGGTTTTTCCGCCGGCGTCGAGCTGGGAGGGGTTTCGGTCTACCTGTCGGAGATCGCCACGCCAGGACGC
AAAGGCTTCTTCGTCAGTTGGCAGTCGGCCAGCCAGCAGGTCGCGGTGGTCTTCGCCGGCCTGCTCGGCGTG
CTCCTCAACCAGTGGCTGAGCCCGCAGGACATGGGCGAATGGGGCTGGCGCGTACCGTTCTTCATCGGCTGC
CTGATCGTTCCGGCGCTGTTCGTCATCCGTCGCTCGCTGGAGGAAACGCCGGAGTTCGAGGCGCGCACCCAC
CGACCGTCGCTGTCGCAGGTGCTGCGCTCCATCGGGCAGAACTTCGGCGTAGTGCTGGCCGGTACCGCGATG
GTGGTGATGACCACCGTATCGTTCTACCTGATCACCGCCTACACGCCGACCTTCGGCAAGAATGAGTTGCAA
CTGTCGGACCTCGACAGCCTGCTGGTGACCATGTGCATCGGCCTGTCCAACTTCATCTGGCTGCCGGTGATG
GGCGCGTTCTCCGACCGCATCGGGCGCAAGCCATTGTTGCTGGGTGCCTCCGCGCTGGCACTGCTGACCGCC
TACCCGGCGCTGTCCTGGCTGGTCCGCGAGCCGAGTTTCGCCGGCCTGCTCGGCGTCGAGTTGTGGCTGTCG
TTCCTCTACAGCAGCTACAACGGCGCGATGGTGGTGGCGTTGACCGAGGTGATGCCGGCGGACGTGCGCACC
ACCGGTTTCTCCCTCGCCTACAGCCTGGCTACGGCGACCTTCGGTGGTTTCACCCCGGCGGTCTGCACCTGG
CTGATCCACACCCTGGACAACCGCGCGGCCCCGGGCATCTGGCTCAGTGGCGCGGCGCTGCTGGGGCTGCTC
GCCACCCTGGTGCTGTTCCGCCGGCGCGGCAAGGAGATCGCCGAGGCCGAGCTGGCCAACGCCTGA
Translation:
MASNKSKGVAIFRVVSGNFLEMYDFMVYGFYATAIAKTFFPGDNPFASLMLSLATFGAGFLMRPLGAVFLGA
YIDRHGRRQGLIITLGLMAMGTLLIAFVPGYATLGVAAPLLVLFGRLLQGFSAGVELGGVSVYLSEIATPGR
KGFFVSWQSASQQVAVVFAGLLGVLLNQWLSPQDMGEWGWRVPFFIGCLIVPALFVIRRSLEETPEFEARTH
RPSLSQVLRSIGQNFGVVLAGTAMVVMTTVSFYLITAYTPTFGKNELQLSDLDSLLVTMCIGLSNFIWLPVM
GAFSDRIGRKPLLLGASALALLTAYPALSWLVREPSFAGLLGVELWLSFLYSSYNGAMVVALTEVMPADVRT
TGFSLAYSLATATFGGFTPAVCTWLIHTLDNRAAPGIWLSGAALLGLLATLVLFRRRGKEIAEAELANA*
AnnotationID:1291GeneID:1282
AnnotatorUID: diggins
Modification Date/Time: 2005-03-21 06:06:29
Gene Name:citA
Confidence Code:2
GeneProduct:citrate transporter
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(27) Transport of small molecules
Alternate Gene Product Name:citrate utilization protein A
Functional Category Confidence Code:5
COGs:pfam00083
Secondary Functional Category(ies):membrane proteins
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAO55622.1| (AE016863) major facilitator family transporter [Pseudomonas
           syringae pv. tomato str. DC3000]
          Length = 432

 Score =  711 bits (1834), Expect = 0.0
 Identities = 351/428 (82%), Positives = 391/428 (91%), Gaps = 1/428 (0%)

>emb|CAD16768.1| (AL646073) PROBABLE SUGAR TRANSPORTER TRANSMEMBRANE PROTEIN
           [Ralstonia solanacearum]
          Length = 431

 Score =  644 bits (1661), Expect = 0.0
 Identities = 315/418 (75%), Positives = 362/418 (86%)

>gb|AAA98398.1| (M22041) citrate utilization protein A [Escherichia coli]
          Length = 431

 Score =  528 bits (1360), Expect = e-149
 Identities = 259/419 (61%), Positives = 321/419 (76%), Gaps = 2/419 (0%)








 
Structural Features:
pfam00083, Sugar_tr, Sugar (and other) transporter.
CD-Length = 448 residues,  98.4% aligned
Score =  142 bits (360), Expect = 6e-35
Identities = 107/457 (23%), Positives = 182/457 (39%), Gaps = 61/457 (13%)






   
Genomic Context:






   
Comment:


















    ReferenceID:2567




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:19710




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:33230




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:1282_cdd.html


    		




 
URL:

















    ReferenceID:35617




  
Author/Investigator(s):
        Shimamoto T, Izawa H, Daimon H, Ishiguro N, Shinagawa M, Sakano Y, Tsuda M, Tsuchiya T.





  
Title:
        Cloning and nucleotide sequence of the gene (citA) encoding a citrate carrier from Salmonella typhimurium.




  
PubMed:
        1718953


MedLine:
        




    
Source:
J Biochem (Tokyo). 1991 Jul;110(1):22-8.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=1718953

















    ReferenceID:35618




  
Author/Investigator(s):
        Daimon H, Ishiguro N, Shinagawa M, Sato G.





  
Title:
        Nucleotide sequence of a citrate utilization gene from Citrobacter amalonaticus.




  
PubMed:
        2644212


MedLine:
        




    
Source:
J Bacteriol. 1989 Jan;171(1):620-3.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=2644212









           
Homologs By Global Alignment 

           

       



     
Gene ID:1282
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
2339
                           PA5476_tr
                           translation of PA5476
                           429
                           99.99
                           99.99
                           0
                           2180.0
     

     
     
75218
                           gb|AAG08861.1|AE004960_4
                           (AE004960) citrate transporter [Pseudomonas aeruginosa PAO1]
                           429
                           99.99
                           99.99
                           0
                           2180.0
     

     
     
75219
                           gb|AAO55622.1|
                           (AE016863) major facilitator family transporter [Pseudomonas syringae pv. tomato str. DC3000]
                           432
                           81.94
                           90.97
                           3
                           1837.0
     

     
     
75220
                           emb|CAD16768.1|
                           (AL646073) PROBABLE SUGAR TRANSPORTER TRANSMEMBRANE PROTEIN [Ralstonia solanacearum]
                           432
                           73.14
                           84.25
                           4
                           1650.0
     

     
     
75221
                           emb|CAD16769.1|
                           (AL646073) PROBABLE TRANSPORTER TRANSMEMBRANE PROTEIN [Ralstonia solanacearum]
                           438
                           69.86
                           80.13
                           14
                           1570.0
     

     






      

          
GC ORFID: 44284How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA5476Glimmer Score: 

          
Start: 5704015Stop: 5702726

          
Length: 1290


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA5476 translation of PA5476Homolog Bit Score 844.0

          
Other Homologs:  psyr_15may02_Scaffold1_revised_gene521 (ORF: BLASTX 721.0), psyr_15may02_Scaffold17_revised_gene2820 (ORF: BLASTX 547.0), psyr_15may02_Scaffold13_revised_gene2170 (ORF: BLASTX 305.0), t_PA5476 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGGCCTCTAATAAATCCAAAGGCGTCGCCATTTTCCGTGTGGTCAGTGGCAACTTCCTCGAGATGTACGAC 
TTCATGGTGTATGGCTTCTACGCCACCGCCATCGCCAAGACCTTCTTCCCCGGCGACAACCCGTTCGCCTCC 
CTGATGCTGTCCCTCGCCACCTTTGGCGCCGGCTTCCTGATGCGGCCGTTGGGTGCGGTGTTCCTGGGCGCC 
TACATCGATCGCCACGGCCGCCGCCAGGGCCTGATCATCACCCTCGGCCTGATGGCCATGGGCACCCTGCTG 
ATCGCCTTCGTTCCCGGCTATGCCACGCTGGGCGTGGCGGCGCCGTTGCTGGTGCTGTTCGGCCGGCTCCTG 
CAGGGTTTTTCCGCCGGCGTCGAGCTGGGAGGGGTTTCGGTCTACCTGTCGGAGATCGCCACGCCAGGACGC 
AAAGGCTTCTTCGTCAGTTGGCAGTCGGCCAGCCAGCAGGTCGCGGTGGTCTTCGCCGGCCTGCTCGGCGTG 
CTCCTCAACCAGTGGCTGAGCCCGCAGGACATGGGCGAATGGGGCTGGCGCGTACCGTTCTTCATCGGCTGC 
CTGATCGTTCCGGCGCTGTTCGTCATCCGTCGCTCGCTGGAGGAAACGCCGGAGTTCGAGGCGCGCACCCAC 
CGACCGTCGCTGTCGCAGGTGCTGCGCTCCATCGGGCAGAACTTCGGCGTAGTGCTGGCCGGTACCGCGATG 
GTGGTGATGACCACCGTATCGTTCTACCTGATCACCGCCTACACGCCGACCTTCGGCAAGAATGAGTTGCAA 
CTGTCGGACCTCGACAGCCTGCTGGTGACCATGTGCATCGGCCTGTCCAACTTCATCTGGCTGCCGGTGATG 
GGCGCGTTCTCCGACCGCATCGGGCGCAAGCCATTGTTGCTGGGTGCCTCCGCGCTGGCACTGCTGACCGCC 
TACCCGGCGCTGTCCTGGCTGGTCCGCGAGCCGAGTTTCGCCGGCCTGCTCGGCGTCGAGTTGTGGCTGTCG 
TTCCTCTACAGCAGCTACAACGGCGCGATGGTGGTGGCGTTGACCGAGGTGATGCCGGCGGACGTGCGCACC 
ACCGGTTTCTCCCTCGCCTACAGCCTGGCTACGGCGACCTTCGGTGGTTTCACCCCGGCGGTCTGCACCTGG 
CTGATCCACACCCTGGACAACCGCGCGGCCCCGGGCATCTGGCTCAGTGGCGCGGCGCTGCTGGGGCTGCTC 
GCCACCCTGGTGCTGTTCCGCCGGCGCGGCAAGGAGATCGCCGAGGCCGAGCTGGCCAACGCCTGA