ViewGene
Locus Name:
GeneID:
Location:

GeneID:1281
TrivialName: PA14_72340
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_72340
PAO1 Ortholog Locus:PA5479
Sequence Length:1335
Protein Length:444
Start: 6444428
Stop: 6443094
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG08864.1|AE004960_7 (AE004960) proton-glutamate symporter [Pseudomonas aeruginosa PAO1]
Identities = 444/444 (100%), Positives = 444/444 (100%)
Sequence:
ATGGGTAAAGCCAAGCTAAGCCTCGCCTGGCAGATCGTGATCGGTCTGGCACTCGGTATCGCCGTCGGCGCA
GTGCTCAACCATTTCAGCGCCGAAAAGGCCTGGTGGATCGCCAACGTGCTGCAACCGGCCGGCGACATCTTC
ATCCGTCTGATCAAGATGATCGTGGTGCCGATCGTGATTTCCTCGCTGGTGGTCGGCATCGCCGGCGTCGGC
GACGCGAAGAAGCTTGGCCGCATCGGCCTGAAGACCATCATCTACTTCGAGATCGTCACCACCGTCGCCATC
GTCGTCGGCCTGCTGCTGGCCAACCTGTTCCAGCCCGGCACCGGCATCGACATGAGCACCCTGGGTACCGTG
GACATTTCCCAGTACCAGAAGACCACCCAGGAAGTGCAGCATGACCATGCGTTCATCGCGACCATCCTCAAC
CTGATTCCCTCCAACGTCTTCGCCGCCATCGCCCGTGGCGAGATGCTGCCGATCATCTTCTTCTCGGTGATG
TTCGGCCTCGGCCTGTCGAGCCTGCCCAACGACCTGCGCGAGCCGCTGGTGAAGGTGTTCCAGGGCGTTTCC
GAGACCATGTTCAAGGTCACCCACATGATCATGAAGTACGCCCCTATCGGTGTCTTCGCCCTGATCGCGGTG
ACCGTCGCCAACTTCGGCTTCGCCTCGCTGCTGCCGCTGGCCAAGCTGGTGATCCTGGTCTACGTGGCCATC
GTCTTCTTCGCCCTGGTGGTGCTGGGCCTGATCGCGCGGATGTTCGGCTTCTCGATCATGCGCCTGATCCGC
ATCTTCAAGGACGACCTGGTCCTCGCCTATTCCACCGCCAGCTCGGAAACCGTGCTGCCGCGCATCATCGAG
AAAATGGAAGCCTACGGCGCGCCGAAGGCGATCAGCAGCTTCGTGGTGCCCACCGGCTACTCCTTCAACCTC
GATGGCTCGACCCTGTACCAGAGCATCGCGGCGATCTTCATCGCGCAGCTCTACGGCATCGACCTGTCCATC
GGCCAGCAGATCATGCTGGTGCTGACCCTGATGGTGACCTCCAAGGGTATCGCCGGCGTGCCGGGCGTGTCC
TTCGTGGTGCTGCTCGCCACCCTGGGCAGCGTCGGCATCCCGCTCGAGGGCCTGGCCTTCATCGCCGGCGTG
GACCGCATCATGGACATGGCGCGTACCGCCCTCAACGTGATCGGCAATGCCCTCGCGGTGCTGGTCATCTCC
AAGTGGGAGGGCATGTACGACGCGGCCAAGGGCCAGCGCTACTGGGATTCGCTGCCGCACCTGCGGCAGGCG
GTCGGCGAAGCCAAGGGCAAGCAGGCCACCCTCGAATAA
Translation:
MGKAKLSLAWQIVIGLALGIAVGAVLNHFSAEKAWWIANVLQPAGDIFIRLIKMIVVPIVISSLVVGIAGVG
DAKKLGRIGLKTIIYFEIVTTVAIVVGLLLANLFQPGTGIDMSTLGTVDISQYQKTTQEVQHDHAFIATILN
LIPSNVFAAIARGEMLPIIFFSVMFGLGLSSLPNDLREPLVKVFQGVSETMFKVTHMIMKYAPIGVFALIAV
TVANFGFASLLPLAKLVILVYVAIVFFALVVLGLIARMFGFSIMRLIRIFKDDLVLAYSTASSETVLPRIIE
KMEAYGAPKAISSFVVPTGYSFNLDGSTLYQSIAAIFIAQLYGIDLSIGQQIMLVLTLMVTSKGIAGVPGVS
FVVLLATLGSVGIPLEGLAFIAGVDRIMDMARTALNVIGNALAVLVISKWEGMYDAAKGQRYWDSLPHLRQA
VGEAKGKQATLE*
AnnotationID:1290GeneID:1281
AnnotatorUID: diggins
Modification Date/Time: 2005-03-21 07:07:05
Gene Name:gltP
Confidence Code:2
GeneProduct:proton-glutamate symporter
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(27) Transport of small molecules
Alternate Gene Product Name:proton/sodium-glutamate/aspartate symporter
Functional Category Confidence Code:5
COGs:pfam00375,COG1301
Secondary Functional Category(ies):energy metabolism
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAO53779.1| (AE016856) proton/glutamate symporter [Pseudomonas syringae pv.
           tomato str. DC3000]
          Length = 443

 Score =  740 bits (1911), Expect = 0.0
 Identities = 373/439 (84%), Positives = 414/439 (94%)

>gb|AAM84100.1|AE013652_9 (AE013652) glutamate-aspartate symport protein [Yersinia pestis
           KIM]
          Length = 438

 Score =  644 bits (1661), Expect = 0.0
 Identities = 329/427 (77%), Positives = 383/427 (89%), Gaps = 1/427 (0%)

>gb|AAM84100.1|AE013652_9 (AE013652) glutamate-aspartate symport protein [Yersinia pestis
           KIM]
          Length = 438

 Score =  644 bits (1661), Expect = 0.0
 Identities = 329/427 (77%), Positives = 383/427 (89%), Gaps = 1/427 (0%)

>gb|AAN83500.1|AE016771_11 (AE016771) Proton glutamate symport protein [Escherichia coli
           CFT073]
          Length = 437

 Score =  621 bits (1602), Expect = e-177
 Identities = 318/423 (75%), Positives = 371/423 (87%), Gaps = 1/423 (0%)







 
Structural Features:
pfam00375, SDF, Sodium:dicarboxylate symporter family.
CD-Length = 400 residues, 100.0% aligned
Score =  394 bits (1014), Expect = 1e-110
Identities = 204/403 (50%), Positives = 283/403 (70%), Gaps = 3/403 (0%)

COG1301, GltP, Na+/H+-dicarboxylate symporters 
[Energy production and conversion]
CD-Length = 415 residues,  97.6% aligned
Score =  376 bits (966), Expect = 4e-105
Identities = 211/420 (50%), Positives = 291/420 (69%), Gaps = 17/420 (4%)






   
Genomic Context:






   
Comment:


















    ReferenceID:2565




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:19708




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:33232




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:1281_cdd.html


    		




 
URL:

















    ReferenceID:35614




  
Author/Investigator(s):
        Tolner B, Poolman B, Wallace B, Konings WN.





  
Title:
        Revised nucleotide sequence of the gltP gene, which encodes the proton-glutamate-aspartate transport protein of Escherichia coli K-12.




  
PubMed:
        1551855


MedLine:
        




    
Source:
J Bacteriol. 1992 Apr;174(7):2391-3.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=1551855

















    ReferenceID:35615




  
Author/Investigator(s):
        Wallace B, Yang YJ, Hong JS, Lum D.





  
Title:
        Cloning and sequencing of a gene encoding a glutamate and aspartate carrier of Escherichia coli K-12.




  
PubMed:
        1971622


MedLine:
        




    
Source:
J Bacteriol. 1990 Jun;172(6):3214-20.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=1971622









           
Homologs By Global Alignment 

           

       



     
Gene ID:1281
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
2337
                           PA5479_tr
                           translation of PA5479
                           444
                           99.99
                           99.99
                           0
                           2180.0
     

     
     
75147
                           gb|AAG08864.1|AE004960_7
                           (AE004960) proton-glutamate symporter [Pseudomonas aeruginosa PAO1]
                           444
                           99.99
                           99.99
                           0
                           2180.0
     

     
     
75148
                           gb|AAO53779.1|
                           (AE016856) proton/glutamate symporter [Pseudomonas syringae pv. tomato str. DC3000]
                           444
                           84.45
                           93.69
                           1
                           1910.0
     

     
     
75149
                           gb|AAN65771.1|AE016203_4
                           (AE016774) proton/sodium-glutamate/aspartate symporter [Pseudomonas putida KT2440]
                           447
                           81.65
                           90.82
                           8
                           1847.0
     

     
     
75150
                           gb|AAM84100.1|AE013652_9
                           (AE013652) glutamate-aspartate symport protein [Yersinia pestis KIM]
                           445
                           74.60
                           87.19
                           8
                           1661.0
     

     






      

          
GC ORFID: 44283How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA5479Glimmer Score: 

          
Start: 5708117Stop: 5706783

          
Length: 1335


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TAATruncated Stop: 

          
Homolog: t_PA5479 translation of PA5479Homolog Bit Score 844.0

          
Other Homologs:  psyr_15may02_Scaffold2_revised_gene3107 (ORF: BLASTX 751.0), psyr_15may02_Scaffold5_revised_gene6446 (ORF: BLASTX 337.0), t_PA1183 (ORF: BLASTX 334.0), t_PA5479 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGGGTAAAGCCAAGCTAAGCCTCGCCTGGCAGATCGTGATCGGTCTGGCACTCGGTATCGCCGTCGGCGCA 
GTGCTCAACCATTTCAGCGCCGAAAAGGCCTGGTGGATCGCCAACGTGCTGCAACCGGCCGGCGACATCTTC 
ATCCGTCTGATCAAGATGATCGTGGTGCCGATCGTGATTTCCTCGCTGGTGGTCGGCATCGCCGGCGTCGGC 
GACGCGAAGAAGCTTGGCCGCATCGGCCTGAAGACCATCATCTACTTCGAGATCGTCACCACCGTCGCCATC 
GTCGTCGGCCTGCTGCTGGCCAACCTGTTCCAGCCCGGCACCGGCATCGACATGAGCACCCTGGGTACCGTG 
GACATTTCCCAGTACCAGAAGACCACCCAGGAAGTGCAGCATGACCATGCGTTCATCGCGACCATCCTCAAC 
CTGATTCCCTCCAACGTCTTCGCCGCCATCGCCCGTGGCGAGATGCTGCCGATCATCTTCTTCTCGGTGATG 
TTCGGCCTCGGCCTGTCGAGCCTGCCCAACGACCTGCGCGAGCCGCTGGTGAAGGTGTTCCAGGGCGTTTCC 
GAGACCATGTTCAAGGTCACCCACATGATCATGAAGTACGCCCCTATCGGTGTCTTCGCCCTGATCGCGGTG 
ACCGTCGCCAACTTCGGCTTCGCCTCGCTGCTGCCGCTGGCCAAGCTGGTGATCCTGGTCTACGTGGCCATC 
GTCTTCTTCGCCCTGGTGGTGCTGGGCCTGATCGCGCGGATGTTCGGCTTCTCGATCATGCGCCTGATCCGC 
ATCTTCAAGGACGACCTGGTCCTCGCCTATTCCACCGCCAGCTCGGAAACCGTGCTGCCGCGCATCATCGAG 
AAAATGGAAGCCTACGGCGCGCCGAAGGCGATCAGCAGCTTCGTGGTGCCCACCGGCTACTCCTTCAACCTC 
GATGGCTCGACCCTGTACCAGAGCATCGCGGCGATCTTCATCGCGCAGCTCTACGGCATCGACCTGTCCATC 
GGCCAGCAGATCATGCTGGTGCTGACCCTGATGGTGACCTCCAAGGGTATCGCCGGCGTGCCGGGCGTGTCC 
TTCGTGGTGCTGCTCGCCACCCTGGGCAGCGTCGGCATCCCGCTCGAGGGCCTGGCCTTCATCGCCGGCGTG 
GACCGCATCATGGACATGGCGCGTACCGCCCTCAACGTGATCGGCAATGCCCTCGCGGTGCTGGTCATCTCC 
AAGTGGGAGGGCATGTACGACGCGGCCAAGGGCCAGCGCTACTGGGATTCGCTGCCGCACCTGCGGCAGGCG 
GTCGGCGAAGCCAAGGGCAAGCAGGCCACCCTCGAATAA