ViewGene
Locus Name:
GeneID:
Location:

GeneID:1274
TrivialName: PA14_30330
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_30330
PAO1 Ortholog Locus:PA2612
Sequence Length:1281
Protein Length:426
Start: 2627215
Stop: 2628495
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG06000.1|AE004689_12 (AE004689) seryl-tRNA synthetase [Pseudomonas aeruginosa PAO1]
Identities = 426/426 (100%), Positives = 426/426 (100%)
Sequence:
ATGCTCGATCCCAAACTGGTACGCACCCAGCCGCAAGAAGTGGCTGCCCGCCTGGCCACCCGTGGCTTCCAA
CTGGATGTCGCACGCATCGAAGCGCTGGAAGAGCAGCGCAAGTCGGTACAGACCCGTACCGAGCAGCTGCAA
GCCGAGCGCAACGCGCGTTCCAAGGCGATCGGCCAGGCCAAGCAGCGTGGCGAGGACATCGCGCCGCTGCTG
GCCGATGTCGACCGCATGGGCTCCGAACTGGAGGAAGGCAAGCGCCAGCTGGACGCTATCCAGGGCGAACTG
GATGCCATGCTGCTGGGCATTCCCAACCTGCCGCACGAGTCGGTGCCGGTCGGTGCCGACGAGGACGCCAAC
GTCGAAGTGCGTCGCTGGGGCACGCCGAAGACCTTCGATTTCGAGGTCAAGGACCATGTCGCCCTCGGTGAA
CGGCACGGCTGGCTGGATTTCGAGACCGCCGCCAAGCTTTCCGGCGCGCGCTTCGCCCTCATGCGCGGCCCG
ATCGCCCGCCTGCATCGCGCCCTGGCGCAGTTCATGATCAACCTGCATACCGCCGAGCATGGCTACGAGGAG
GCCTACACCCCCTATCTCGTCCAGGCTCCGGCGCTGCAGGGCACCGGCCAGTTGCCGAAGTTCGAGGAGGAC
CTGTTCAAGATCGGCCGCGACGGCGAAGCCGACCTCTACCTGATCCCGACCGCCGAGGTTTCGCTGACCAAC
ATCGTCTCCGGGCAGATCCTCGATGCCAAGCAGTTGCCGCTGAAGTTCGTCGCCCATACCCCGTGCTTCCGC
AGCGAGGCTGGTGCCTCCGGGCGCGATACCCGCGGCATGATCCGCCAGCACCAGTTCGACAAGGTGGAGATG
GTGCAGATCGTCGATCCGGCCACCTCCTACGAGGCGCTGGAAGGCCTGACCGCTAACGCCGAGCGCGTCCTG
CAACTGCTCGAGCTGCCGTATCGGGTGCTGGCGCTGTGCACCGGCGACATGGGCTTCGGTGCGACCAAGACC
TACGACCTGGAAGTCTGGGTTCCCAGCCAGGACAAGTACCGCGAGATTTCCTCCTGCTCCAACTGCGGCGAC
TTCCAGGCCCGGCGCATGCAGGCCCGCTACCGCAATCCGGAAACCGGCAAGCCCGAGCTGGTGCACACCCTC
AACGGCTCCGGCCTGGCCGTCGGCCGCACCCTGGTGGCGGTGCTGGAGAACTACCAGCAGGCGGACGGCTCG
ATCCGTGTGCCGGAAGTCCTCAAGCCCTACATGGCTGGCATCGAGGTGATCGGCTGA
Translation:
MLDPKLVRTQPQEVAARLATRGFQLDVARIEALEEQRKSVQTRTEQLQAERNARSKAIGQAKQRGEDIAPLL
ADVDRMGSELEEGKRQLDAIQGELDAMLLGIPNLPHESVPVGADEDANVEVRRWGTPKTFDFEVKDHVALGE
RHGWLDFETAAKLSGARFALMRGPIARLHRALAQFMINLHTAEHGYEEAYTPYLVQAPALQGTGQLPKFEED
LFKIGRDGEADLYLIPTAEVSLTNIVSGQILDAKQLPLKFVAHTPCFRSEAGASGRDTRGMIRQHQFDKVEM
VQIVDPATSYEALEGLTANAERVLQLLELPYRVLALCTGDMGFGATKTYDLEVWVPSQDKYREISSCSNCGD
FQARRMQARYRNPETGKPELVHTLNGSGLAVGRTLVAVLENYQQADGSIRVPEVLKPYMAGIEVIG*
AnnotationID:1283GeneID:1274
AnnotatorUID: diggins
Modification Date/Time: 2005-04-29 11:11:47
Gene Name:serS
Confidence Code:2
GeneProduct:seryl-tRNA synthetase
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(26) Translation, post-translational modification, degradation
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:COG0172,cd00770
Secondary Functional Category(ies):amino acid biosynthesis and metabolims
EC Number:6.1.1.11
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAO56823.1| (AE016868) seryl-tRNA synthetase [Pseudomonas syringae pv. tomato
           str. DC3000]
          Length = 426

 Score =  752 bits (1941), Expect = 0.0
 Identities = 377/426 (88%), Positives = 396/426 (92%)

>gb|AAN69594.1|AE016593_2 (AE016788) seryl-tRNA synthetase [Pseudomonas putida KT2440]
          Length = 426

 Score =  733 bits (1893), Expect = 0.0
 Identities = 367/425 (86%), Positives = 393/425 (92%)

>gb|AAG55380.1|AE005278_6 (AE005278) serine tRNA synthetase; also charges selenocystein tRNA
           with serine [Escherichia coli O157:H7 EDL933]
          Length = 430

 Score =  565 bits (1455), Expect = e-160
 Identities = 283/430 (65%), Positives = 336/430 (78%), Gaps = 4/430 (0%)






 
Structural Features:
COG0172, SerS, Seryl-tRNA synthetase 
[Translation, ribosomal structure and biogenesis]
CD-Length = 429 residues,  99.3% aligned
Score =  600 bits (1548), Expect = 1e-172
Identities = 264/430 (61%), Positives = 323/430 (75%), Gaps = 8/430 (1%)

cd00770, SerRS_core, Seryl-tRNA synthetase (SerRS) class II core catalytic domain. 
CD-Length = 297 residues,  99.7% aligned
Score =  458 bits (1180), Expect = 6e-130







   
Genomic Context:






   
Comment:


















    ReferenceID:2551




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:19694




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:33176




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:1274_cdd.html


    		




 
URL:

















    ReferenceID:34545




  
Author/Investigator(s):
        Weygand-Durasevic I, Johnson-Burke D, Soll D.





  
Title:
        Cloning and characterization of the gene coding for cytoplasmic seryl-tRNA synthetase from Saccharomyces cerevisiae.




  
PubMed:
        3031581


MedLine:
        




    
Source:
Nucleic Acids Res. 1987 Mar 11;15(5):1887-904.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=3031581

















    ReferenceID:34546




  
Author/Investigator(s):
        Hartlein M, Madern D, Leberman R.





  
Title:
        Cloning and characterization of the gene for Escherichia coli seryl-tRNA synthetase.




  
PubMed:
        3029694


MedLine:
        




    
Source:
Nucleic Acids Res. 1987 Feb 11;15(3):1005-17.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=3029694









           
Homologs By Global Alignment 

           

       



     
Gene ID:1274
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
2320
                           PA2612_tr
                           translation of PA2612
                           426
                           99.99
                           99.99
                           0
                           2183.0
     

     
     
74824
                           gb|AAG06000.1|AE004689_12
                           (AE004689) seryl-tRNA synthetase [Pseudomonas aeruginosa PAO1]
                           426
                           99.99
                           99.99
                           0
                           2183.0
     

     
     
74825
                           gb|AAO56823.1|
                           (AE016868) seryl-tRNA synthetase [Pseudomonas syringae pv. tomato str. DC3000]
                           426
                           88.49
                           92.95
                           0
                           1941.0
     

     
     
74826
                           gb|AAN69594.1|AE016593_2
                           (AE016788) seryl-tRNA synthetase [Pseudomonas putida KT2440]
                           426
                           86.15
                           92.25
                           0
                           1891.0
     

     
     
74835
                           gb|AAC21787.1|
                           (U32696) seryl-tRNA synthetase (serS) [Haemophilus influenzae Rd] [Haemophilus influenzae Rd KW20]
                           429
                           65.96
                           77.38
                           3
                           1445.0
     

     






      

          
GC ORFID: 44276How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA2612Glimmer Score: 

          
Start: 2326711Stop: 2325431

          
Length: 1281


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA2612 translation of PA2612Homolog Bit Score 845.0

          
Other Homologs:  psyr_15may02_Scaffold17_revised_gene2816 (ORF: BLASTX 753.0), t_PA2612 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGCTCGATCCCAAACTGGTACGCACCCAGCCGCAAGAAGTGGCTGCCCGCCTGGCCACCCGTGGCTTCCAA 
CTGGATGTCGCACGCATCGAAGCGCTGGAAGAGCAGCGCAAGTCGGTACAGACCCGTACCGAGCAGCTGCAA 
GCCGAGCGCAACGCGCGTTCCAAGGCGATCGGCCAGGCCAAGCAGCGTGGCGAGGACATCGCGCCGCTGCTG 
GCCGATGTCGACCGCATGGGCTCCGAACTGGAGGAAGGCAAGCGCCAGCTGGACGCTATCCAGGGCGAACTG 
GATGCCATGCTGCTGGGCATTCCCAACCTGCCGCACGAGTCGGTGCCGGTCGGTGCCGACGAGGACGCCAAC 
GTCGAAGTGCGTCGCTGGGGCACGCCGAAGACCTTCGATTTCGAGGTCAAGGACCATGTCGCCCTCGGTGAA 
CGGCACGGCTGGCTGGATTTCGAGACCGCCGCCAAGCTTTCCGGCGCGCGCTTCGCCCTCATGCGCGGCCCG 
ATCGCCCGCCTGCATCGCGCCCTGGCGCAGTTCATGATCAACCTGCATACCGCCGAGCATGGCTACGAGGAG 
GCCTACACCCCCTATCTCGTCCAGGCTCCGGCGCTGCAGGGCACCGGCCAGTTGCCGAAGTTCGAGGAGGAC 
CTGTTCAAGATCGGCCGCGACGGCGAAGCCGACCTCTACCTGATCCCGACCGCCGAGGTTTCGCTGACCAAC 
ATCGTCTCCGGGCAGATCCTCGATGCCAAGCAGTTGCCGCTGAAGTTCGTCGCCCATACCCCGTGCTTCCGC 
AGCGAGGCTGGTGCCTCCGGGCGCGATACCCGCGGCATGATCCGCCAGCACCAGTTCGACAAGGTGGAGATG 
GTGCAGATCGTCGATCCGGCCACCTCCTACGAGGCGCTGGAAGGCCTGACCGCTAACGCCGAGCGCGTCCTG 
CAACTGCTCGAGCTGCCGTATCGGGTGCTGGCGCTGTGCACCGGCGACATGGGCTTCGGTGCGACCAAGACC 
TACGACCTGGAAGTCTGGGTTCCCAGCCAGGACAAGTACCGCGAGATTTCCTCCTGCTCCAACTGCGGCGAC 
TTCCAGGCCCGGCGCATGCAGGCCCGCTACCGCAATCCGGAAACCGGCAAGCCCGAGCTGGTGCACACCCTC 
AACGGCTCCGGCCTGGCCGTCGGCCGCACCCTGGTGGCGGTGCTGGAGAACTACCAGCAGGCGGACGGCTCG 
ATCCGTGTGCCGGAAGTCCTCAAGCCCTACATGGCTGGCATCGAGGTGATCGGCTGA