ViewGene
Locus Name:
GeneID:
Location:

GeneID:1264
TrivialName: PA14_65960
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_65960
PAO1 Ortholog Locus:PA4988
Sequence Length:1278
Protein Length:425
Start: 5876495
Stop: 5875218
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG08373.1|AE004911_8 (AE004911) 3-deoxy-D-manno-octulosonic-acid (KDO) transferase [Pseudomonas aeruginosa PAO1]
Identities = 425/425 (100%), Positives = 425/425 (100%)
Sequence:
ATGAACCGTACCCTCTATACCCTGCTGTTCCACCTCGGCTTGCCGCTGGTGGCGTTGCGCCTGTGGTGGCGC
GCGCGCCAGGCGCCGGCCTACGCCAAGCGCATCGGCGAGCGTTTTTCCCTGTCCTTGCCCGAGGTGCCGCCC
GGCGGCATCTGGGTGCACGCCGTCTCGGTGGGCGAGAGCATCGCCGCCGCGCCGATGGTGCGGGCGCTGCTG
GAGCGCCATCCGCAGCTGCCGGTCACCGTCACCTGCATGACCCCGACCGGCTCGGAGCGCATCCGCGCATTG
TTCGGCGAGCAGGTCCGGCACTGCTATCTGCCCTACGACCTGCCCTGGGCGGCGGCGCGCTTCCTCGACCGG
GTGCGGCCGCGCCTGGCGGTGATCATGGAAACCGAGCTGTGGCCCAACCATATCCATGCCTGCGCCGTGCGC
GGGATCCCGGTGGCGCTGGCCAATGCGCGCCTGTCCGAGCGCTCGGCGCGTGGCTACGCGCGCTTCGCCGGG
CTGACCCGGCCGATGCTCGCCGAGCTGTCCTGGATCGCCGTGCAGACCGAGGCCGAGGCCGAGCGCTTCCGT
CGTCTCGGCGCGCGTCCTGAGTGCGTCAGCGTGACCGGCTCGATCAAGTTCGACCTGCGCATCGACCCGCAG
CTGCCGCTGGCCGCTGCCGCCCTGCGCGAGGAATGGGACGCGACGGCGCGACCGCTGTGGATCGCCGCCAGC
ACCCATGCCGGCGAGGACGAGATCGTCCTGGCAGCCCACCGGCGCCTGCTGGAAACGCGTCCCGACGCGTTG
CTGATCCTGGTGCCGCGCCATCCGGAACGCTTCGCCGGCGTCCACGAGCTATGCCGCCGCGAAGGCTTCGCC
ACGGTCCGGCGCTCCGGCGGCGAGCCGGTGGCACGCGCGACCCAGGTGCTGCTCGGCGATACCATGGGCGAG
CTGCTGTTCCTCTATGCCCTGGCCGACATCGCTTTCGTCGGCGGCAGCCTGGTGCCCAACGGCGGGCACAAC
CTGCTGGAGCCGGCGGCCCTGGGCAAGCCGGTGTTCGCCGGTCCGCACCTGTTCAACTTCCTCGACATCGCC
GCGCAATTGCGCGACGCCGGGGCGCTGCTCGAGGTGACGGATGCCGGCGAGCTGTGCGACGGCCTCGCGCGC
CTGTGGGCGCAGCCGGAGGTCGCGACGGCCATGGCCACTGCCGGAGAGAAGGTGCTGCGCAACAACCAGGGC
GCCCTGGAGCGGCTGCTGGCGGGGTTGGCGCGGTTGCTCGGGCGCGGCGGGTAG
Translation:
MNRTLYTLLFHLGLPLVALRLWWRARQAPAYAKRIGERFSLSLPEVPPGGIWVHAVSVGESIAAAPMVRALL
ERHPQLPVTVTCMTPTGSERIRALFGEQVRHCYLPYDLPWAAARFLDRVRPRLAVIMETELWPNHIHACAVR
GIPVALANARLSERSARGYARFAGLTRPMLAELSWIAVQTEAEAERFRRLGARPECVSVTGSIKFDLRIDPQ
LPLAAAALREEWDATARPLWIAASTHAGEDEIVLAAHRRLLETRPDALLILVPRHPERFAGVHELCRREGFA
TVRRSGGEPVARATQVLLGDTMGELLFLYALADIAFVGGSLVPNGGHNLLEPAALGKPVFAGPHLFNFLDIA
AQLRDAGALLEVTDAGELCDGLARLWAQPEVATAMATAGEKVLRNNQGALERLLAGLARLLGRGG*
AnnotationID:1273GeneID:1264
AnnotatorUID: diggins
Modification Date/Time: 2005-04-26 08:08:55
Gene Name:waaA
Confidence Code:2
GeneProduct:3-deoxy-D-manno-octulosonic-acid (KDO) transferase
Cell Localization:()
Synonyms:kdtA
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(7) Cell wall / LPS / capsule
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:COG1519,pfam04413
Secondary Functional Category(ies):
EC Number:2.-.-.-
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAO58406.1| (AE016874) 3-deoxy-D-manno-octulosonic-acid transferase
           [Pseudomonas syringae pv. tomato str. DC3000]
          Length = 447

 Score =  621 bits (1602), Expect = e-177
 Identities = 311/425 (73%), Positives = 353/425 (83%), Gaps = 2/425 (0%)

>gb|AAN70495.1|AE016691_3 (AE016792) 3-deoxy-D-manno-octulosonic-acid transferase
           [Pseudomonas putida KT2440]
          Length = 423

 Score =  589 bits (1518), Expect = e-167
 Identities = 306/423 (72%), Positives = 340/423 (80%), Gaps = 3/423 (0%)

>gb|AAO71276.1| (AE016847) 3-deoxy-D-manno-octulosonic-acid transferase [Salmonella
           enterica subsp. enterica serovar Typhi Ty2]
          Length = 425

 Score =  424 bits (1091), Expect = e-118
 Identities = 221/414 (53%), Positives = 279/414 (67%), Gaps = 1/414 (0%)








 
Structural Features:
COG1519, KdtA, 3-deoxy-D-manno-octulosonic-acid transferase 
[Cell envelope biogenesis, outer membrane]
CD-Length = 419 residues,  99.8% aligned
Score =  519 bits (1338), Expect = 3e-148
Identities = 226/421 (53%), Positives = 274/421 (65%), Gaps = 5/421 (1%)

pfam04413, Glycos_transf_N, 3-Deoxy-D-manno-octulosonic-acid transferase (kdotransferase). 
CD-Length = 192 residues, 100.0% aligned
Score =  260 bits (666), Expect = 2e-70
Identities = 109/192 (56%), Positives = 131/192 (68%), Gaps = 1/192 (0%)






   
Genomic Context:






   
Comment:


















    ReferenceID:2531




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:19674




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:35092




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:1264_cdd.html


    		




 
URL:

















    ReferenceID:35879




  
Author/Investigator(s):
        Noah C, Brabetz W, Gronow S, Brade H.





  
Title:
        Cloning, sequencing, and functional analysis of three glycosyltransferases involved in the biosynthesis of the inner core region of Klebsiella pneumoniae lipopolysaccharide.




  
PubMed:
        11521078


MedLine:
        




    
Source:
J Endotoxin Res. 2001;7(1):25-33.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=11521078









           
Homologs By Global Alignment 

           

       



     
Gene ID:1264
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
2309
                           PA4988_tr
                           translation of PA4988
                           425
                           99.99
                           99.99
                           0
                           2194.0
     

     
     
74612
                           gb|AAG08373.1|AE004911_8
                           (AE004911) 3-deoxy-D-manno-octulosonic-acid (KDO) transferase [Pseudomonas aeruginosa PAO1]
                           425
                           99.99
                           99.99
                           0
                           2194.0
     

     
     
74614
                           gb|AAN70495.1|AE016691_3
                           (AE016792) 3-deoxy-D-manno-octulosonic-acid transferase [Pseudomonas putida KT2440]
                           427
                           71.66
                           79.62
                           6
                           1520.0
     

     
     
74613
                           gb|AAO58406.1|
                           (AE016874) 3-deoxy-D-manno-octulosonic-acid transferase [Pseudomonas syringae pv. tomato str. DC3000]
                           447
                           69.57
                           78.97
                           22
                           1600.0
     

     
     
74618
                           gb|AAC69669.1|
                           (AF019745) Kdo transferase WaaA [Escherichia coli]
                           425
                           52.70
                           66.82
                           3
                           1092.0
     

     






      

          
GC ORFID: 44266How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA4988Glimmer Score: 

          
Start: 5140178Stop: 5138901

          
Length: 1278


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TAGTruncated Stop: 

          
Homolog: t_PA4988 translation of PA4988Homolog Bit Score 849.0

          
Other Homologs:  psyr_15may02_Scaffold2_revised_gene3403 (ORF: BLASTX 649.0), t_PA4988 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGAACCGTACCCTCTATACCCTGCTGTTCCACCTCGGCTTGCCGCTGGTGGCGTTGCGCCTGTGGTGGCGC 
GCGCGCCAGGCGCCGGCCTACGCCAAGCGCATCGGCGAGCGTTTTTCCCTGTCCTTGCCCGAGGTGCCGCCC 
GGCGGCATCTGGGTGCACGCCGTCTCGGTGGGCGAGAGCATCGCCGCCGCGCCGATGGTGCGGGCGCTGCTG 
GAGCGCCATCCGCAGCTGCCGGTCACCGTCACCTGCATGACCCCGACCGGCTCGGAGCGCATCCGCGCATTG 
TTCGGCGAGCAGGTCCGGCACTGCTATCTGCCCTACGACCTGCCCTGGGCGGCGGCGCGCTTCCTCGACCGG 
GTGCGGCCGCGCCTGGCGGTGATCATGGAAACCGAGCTGTGGCCCAACCATATCCATGCCTGCGCCGTGCGC 
GGGATCCCGGTGGCGCTGGCCAATGCGCGCCTGTCCGAGCGCTCGGCGCGTGGCTACGCGCGCTTCGCCGGG 
CTGACCCGGCCGATGCTCGCCGAGCTGTCCTGGATCGCCGTGCAGACCGAGGCCGAGGCCGAGCGCTTCCGT 
CGTCTCGGCGCGCGTCCTGAGTGCGTCAGCGTGACCGGCTCGATCAAGTTCGACCTGCGCATCGACCCGCAG 
CTGCCGCTGGCCGCTGCCGCCCTGCGCGAGGAATGGGACGCGACGGCGCGACCGCTGTGGATCGCCGCCAGC 
ACCCATGCCGGCGAGGACGAGATCGTCCTGGCAGCCCACCGGCGCCTGCTGGAAACGCGTCCCGACGCGTTG 
CTGATCCTGGTGCCGCGCCATCCGGAACGCTTCGCCGGCGTCCACGAGCTATGCCGCCGCGAAGGCTTCGCC 
ACGGTCCGGCGCTCCGGCGGCGAGCCGGTGGCACGCGCGACCCAGGTGCTGCTCGGCGATACCATGGGCGAG 
CTGCTGTTCCTCTATGCCCTGGCCGACATCGCTTTCGTCGGCGGCAGCCTGGTGCCCAACGGCGGGCACAAC 
CTGCTGGAGCCGGCGGCCCTGGGCAAGCCGGTGTTCGCCGGTCCGCACCTGTTCAACTTCCTCGACATCGCC 
GCGCAATTGCGCGACGCCGGGGCGCTGCTCGAGGTGACGGATGCCGGCGAGCTGTGCGACGGCCTCGCGCGC 
CTGTGGGCGCAGCCGGAGGTCGCGACGGCCATGGCCACTGCCGGAGAGAAGGTGCTGCGCAACAACCAGGGC 
GCCCTGGAGCGGCTGCTGGCGGGGTTGGCGCGGTTGCTCGGGCGCGGCGGGTAG