ViewGene
Locus Name:
GeneID:
Location:

GeneID:1253
TrivialName: PA14_20050
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_20050
PAO1 Ortholog Locus:PA3404
Sequence Length:1356
Protein Length:451
Start: 1728648
Stop: 1730003
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG06792.1|AE004761_8 (AE004761) probable outer membrane protein precursor [Pseudomonas aeruginosa PAO1]
Identities = 449/451 (99%), Positives = 449/451 (99%)
Sequence:
ATGAATCGCCTGCGAGCCTGCCTGCTGTCCTCCGCGCTGCTTTCGGCCTCTTCGGCCCAGGCGCTGGGCCTG
CTGGACGCCTACCAACTGGCGGTGCGCCACGACCCGACCTTCCAGGCGGCGCTCCACGAGCGCCGCGCCGGC
AGCGAAAACCGCGCCATCGGCCGTGCCGGCCTGCTGCCGAGCCTGCGCTACGACTACAACAAGGCGCGCAAC
GACTCCACCGTCAGCCAGGGCGACGCCCGCGTCGAGCGCGACTATCGCAGCTACGCCTCGACCCTCAGCCTG
GAGCAGCCGCTGTTCGACTACGAGGCCTACGCGCGCTACCGCCAGGGCGAGGCCCAGGCGCTGTTCGCCGAC
GAACAGTTCCGCGGGCGCAGCCAGGAACTGGCGGTGCGCCTGTTCGCCGCCTACAGCGAAACCCTGTTCGCC
CGCGAGCAGGTGGTCCTGGCCGAGGCCCAGCGGCGCGCCCTGGAAACCCAGCTGGCGTTCAACCAGCGTGCC
TTCGAGGAAGGCGAGGGCACCCGCACCGACCTGCTGGAGACCCGCGCCCGGCTGAGCCTGACCCGCGCCGAG
GAGATCGCCGCCGGCGATCGTGCGGCGGCGGCGCGGCGTACGCTGGAAGCGATGCTCGGGCAGGCACTGGAG
GATCGCGAACTGGCCGCGCCCATCGAGCGTTTCCCGGCGTTGCGCCTGCAACCGGCGACCTTCGAGGGCTGG
CGCCAGGTGGCCCTGCAACGCAGCGCCGAGCTGGGCGCCCAGCGCCATGCGCTGGAGGCGGCGGCCTACGAG
GTGGAGCGCAATCGTGCCGGCCACCTGCCCAGGCTCAGCCTCTACGCCAGCAGCAGCAAGACGCACTCGGCT
TCGGAAAGCACCTACGAGCAGAAATACGACACCGACAGTGTCGGCCTGCGTCTCAGCCTGCCGCTGTTCGAG
GGCGGGCGGGTGTCGGCGGCGACCCGCCAGGCCGGCGACAAGTACGCCCAGGCCCAGGCCGAGCTGGATGCC
CAGGTGGCCAGTGTGATCAATGACCTGCACAGCCAGTTCGACCTGACCGCCAGCAGCCTGGCCAAGGTGCGT
GCCTATGAGATGGCGGTGGCGGCGGCGCGCGAACAGGTGACGGCGACGCGGCGTAGCGTCGCCGGCGGGGAG
CGGGTCAATCGCGACGTGCTGGACGCCGAACAGCAGTTCTACGGCGCCCGCCGCGACCTGGCGGAAGCGCGC
TACGCCTACCTTAACGCCTGGCTGCGCCTGCGCCAGCTGGCGGGGGTGCTGGAAGACCGCGACCTAGCGGTA
CTGGCCGCCTATTTCGGCGCCGGCGAAGGACGCGCGCAGGTCGCCGCTGCCATCAGATAG
Translation:
MNRLRACLLSSALLSASSAQALGLLDAYQLAVRHDPTFQAALHERRAGSENRAIGRAGLLPSLRYDYNKARN
DSTVSQGDARVERDYRSYASTLSLEQPLFDYEAYARYRQGEAQALFADEQFRGRSQELAVRLFAAYSETLFA
REQVVLAEAQRRALETQLAFNQRAFEEGEGTRTDLLETRARLSLTRAEEIAAGDRAAAARRTLEAMLGQALE
DRELAAPIERFPALRLQPATFEGWRQVALQRSAELGAQRHALEAAAYEVERNRAGHLPRLSLYASSSKTHSA
SESTYEQKYDTDSVGLRLSLPLFEGGRVSAATRQAGDKYAQAQAELDAQVASVINDLHSQFDLTASSLAKVR
AYEMAVAAAREQVTATRRSVAGGERVNRDVLDAEQQFYGARRDLAEARYAYLNAWLRLRQLAGVLEDRDLAV
LAAYFGAGEGRAQVAAAIR*
AnnotationID:1262GeneID:1253
AnnotatorUID: diggins
Modification Date/Time: 2005-04-18 11:11:54
Gene Name:
Confidence Code:3
GeneProduct:putative outer membrane protein
Cell Localization:()
Synonyms:hasF
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(19) Protein secretion/export apparatus
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:pfam02321,COG1538
Secondary Functional Category(ies):
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>dbj|BAA88494.1| (AB023289) outer membrane protein [Pseudomonas fluorescens]
          Length = 442

 Score =  500 bits (1288), Expect = e-141
 Identities = 262/438 (59%), Positives = 334/438 (76%), Gaps = 4/438 (0%)

>gb|AAF01330.1| (AF188365) outer membrane protein [Pseudomonas chlororaphis]
          Length = 453

 Score =  412 bits (1058), Expect = e-114
 Identities = 217/444 (48%), Positives = 297/444 (66%), Gaps = 6/444 (1%)

>gb|AAO56806.1| (AE016868) ABC transporter, permease protein [Pseudomonas syringae
           pv. tomato str. DC3000]
          Length = 456

 Score =  403 bits (1036), Expect = e-111
 Identities = 217/447 (48%), Positives = 302/447 (67%), Gaps = 7/447 (1%)








 
Structural Features:
pfam02321, OEP, Outer membrane efflux protein.
CD-Length = 188 residues, 100.0% aligned
Score = 92.0 bits (228), Expect = 1e-19
Identities = 68/188 (36%), Positives = 99/188 (52%), Gaps = 1/188 (0%)

COG1538, TolC, Outer membrane protein 
[Cell envelope biogenesis, outer membrane / 
Intracellular trafficking and secretion]
CD-Length = 457 residues,  93.9% aligned
Score =  139 bits (352), Expect = 5e-34
Identities = 137/432 (31%), Positives = 193/432 (44%), Gaps = 5/432 (1%)






   
Genomic Context:






   
Comment:


















    ReferenceID:2509




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:19652




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:32160




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:1253_cdd.html


    		




 
URL:









           
Homologs By Global Alignment 

           

       



     
Gene ID:1253
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
2283
                           PA3404_tr
                           translation of PA3404
                           451
                           99.55
                           99.55
                           0
                           2205.0
     

     
     
74352
                           gb|AAG06792.1|AE004761_8
                           (AE004761) probable outer membrane protein precursor [Pseudomonas aeruginosa PAO1]
                           451
                           99.55
                           99.55
                           0
                           2205.0
     

     
     
74353
                           dbj|BAA88494.1|
                           (AB023289) outer membrane protein [Pseudomonas fluorescens]
                           455
                           57.58
                           73.40
                           17
                           1287.0
     

     
     
74355
                           emb|CAA45857.1|
                           (X64558) aprF [Pseudomonas aeruginosa]
                           486
                           48.35
                           63.78
                           40
                           1119.0
     

     
     
74354
                           gb|AAG04637.1|AE004554_5
                           (AE004554) Alkaline protease secretion outer membrane protein AprF precursor [Pseudomonas aeruginosa PAO1]
                           486
                           48.35
                           63.78
                           40
                           1119.0
     

     






      

          
GC ORFID: 44255How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA3404Glimmer Score: 

          
Start: 3225284Stop: 3223929

          
Length: 1356


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TAGTruncated Stop: 

          
Homolog: t_PA3404 translation of PA3404Homolog Bit Score 853.0

          
Other Homologs:  t_PA3404 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGAATCGCCTGCGAGCCTGCCTGCTGTCCTCCGCGCTGCTTTCGGCCTCTTCGGCCCAGGCGCTGGGCCTG 
CTGGACGCCTACCAACTGGCGGTGCGCCACGACCCGACCTTCCAGGCGGCGCTCCACGAGCGCCGCGCCGGC 
AGCGAAAACCGCGCCATCGGCCGTGCCGGCCTGCTGCCGAGCCTGCGCTACGACTACAACAAGGCGCGCAAC 
GACTCCACCGTCAGCCAGGGCGACGCCCGCGTCGAGCGCGACTATCGCAGCTACGCCTCGACCCTCAGCCTG 
GAGCAGCCGCTGTTCGACTACGAGGCCTACGCGCGCTACCGCCAGGGCGAGGCCCAGGCGCTGTTCGCCGAC 
GAACAGTTCCGCGGGCGCAGCCAGGAACTGGCGGTGCGCCTGTTCGCCGCCTACAGCGAAACCCTGTTCGCC 
CGCGAGCAGGTGGTCCTGGCCGAGGCCCAGCGGCGCGCCCTGGAAACCCAGCTGGCGTTCAACCAGCGTGCC 
TTCGAGGAAGGCGAGGGCACCCGCACCGACCTGCTGGAGACCCGCGCCCGGCTGAGCCTGACCCGCGCCGAG 
GAGATCGCCGCCGGCGATCGTGCGGCGGCGGCGCGGCGTACGCTGGAAGCGATGCTCGGGCAGGCACTGGAG 
GATCGCGAACTGGCCGCGCCCATCGAGCGTTTCCCGGCGTTGCGCCTGCAACCGGCGACCTTCGAGGGCTGG 
CGCCAGGTGGCCCTGCAACGCAGCGCCGAGCTGGGCGCCCAGCGCCATGCGCTGGAGGCGGCGGCCTACGAG 
GTGGAGCGCAATCGTGCCGGCCACCTGCCCAGGCTCAGCCTCTACGCCAGCAGCAGCAAGACGCACTCGGCT 
TCGGAAAGCACCTACGAGCAGAAATACGACACCGACAGTGTCGGCCTGCGTCTCAGCCTGCCGCTGTTCGAG 
GGCGGGCGGGTGTCGGCGGCGACCCGCCAGGCCGGCGACAAGTACGCCCAGGCCCAGGCCGAGCTGGATGCC 
CAGGTGGCCAGTGTGATCAATGACCTGCACAGCCAGTTCGACCTGACCGCCAGCAGCCTGGCCAAGGTGCGT 
GCCTATGAGATGGCGGTGGCGGCGGCGCGCGAACAGGTGACGGCGACGCGGCGTAGCGTCGCCGGCGGGGAG 
CGGGTCAATCGCGACGTGCTGGACGCCGAACAGCAGTTCTACGGCGCCCGCCGCGACCTGGCGGAAGCGCGC 
TACGCCTACCTTAACGCCTGGCTGCGCCTGCGCCAGCTGGCGGGGGTGCTGGAAGACCGCGACCTAGCGGTA 
CTGGCCGCCTATTTCGGCGCCGGCGAAGGACGCGCGCAGGTCGCCGCTGCCATCAGATAG